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P19319

- NARZ_ECOLI

UniProt

P19319 - NARZ_ECOLI

Protein

Respiratory nitrate reductase 2 alpha chain

Gene

narZ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 5 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth.
    The alpha chain is the actual site of nitrate reduction.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.By similarity
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501Iron-sulfur (4Fe-4S); via pros nitrogenPROSITE-ProRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi58 – 581Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi93 – 931Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi223 – 2231MolybdenumBy similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: EcoCyc
    3. molybdenum ion binding Source: EcoCyc
    4. nitrate reductase activity Source: UniProtKB-EC
    5. oxidoreductase activity, acting on NAD(P)H Source: RefGenome
    6. protein binding Source: IntAct

    GO - Biological processi

    1. anaerobic respiration Source: EcoCyc
    2. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciEcoCyc:NARZ-MONOMER.
    ECOL316407:JW1463-MONOMER.
    MetaCyc:NARZ-MONOMER.

    Protein family/group databases

    TCDBi5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Respiratory nitrate reductase 2 alpha chain (EC:1.7.99.4)
    Gene namesi
    Name:narZ
    Ordered Locus Names:b1468, JW1463
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10648. narZ.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: UniProtKB
    2. nitrate reductase complex Source: InterPro
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 12461245Respiratory nitrate reductase 2 alpha chainPRO_0000063234Add
    BLAST

    Proteomic databases

    PaxDbiP19319.
    PRIDEiP19319.

    Expressioni

    Gene expression databases

    GenevestigatoriP19319.

    Interactioni

    Subunit structurei

    Tetramer composed of an alpha, a beta and 2 gamma chains. Alpha and beta are catalytic chains; gamma chain is involved in binding the enzyme complex to the cytoplasmic membrane.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    narHP113494EBI-547262,EBI-555067
    narJP0AF263EBI-547262,EBI-555043
    narWP193173EBI-547262,EBI-555088

    Protein-protein interaction databases

    DIPiDIP-10324N.
    IntActiP19319. 29 interactions.
    MINTiMINT-1261362.
    STRINGi511145.b1468.

    Structurei

    3D structure databases

    ProteinModelPortaliP19319.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 107654Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5013.
    HOGENOMiHOG000237341.
    KOiK00370.
    OMAiVKQEPVL.
    OrthoDBiEOG6RC3JS.
    PhylomeDBiP19319.

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR006468. NarG.
    IPR028189. Nitr_red_alph_N.
    [Graphical view]
    PfamiPF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF14710. Nitr_red_alph_N. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01580. narG. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKLLDRFRY FKQKGETFAD GHGQVMHSNR DWEDSYRQRW QFDKIVRSTH     50
    GVNCTGSCSW KIYVKNGLVT WEIQQTDYPR TRPDLPNHEP RGCPRGASYS 100
    WYLYSANRLK YPLIRKRLIE LWREALKQHS DPVLAWASIM NDPQKCLSYK 150
    QVRGRGGFIR SNWQELNQLI AAANVWTIKT YGPDRVAGFS PIPAMSMVSY 200
    AAGTRYLSLL GGTCLSFYDW YCDLPPASPM TWGEQTDVPE SADWYNSSYI 250
    IAWGSNVPQT RTPDAHFFTE VRYKGTKTIA ITPDYSEVAK LCDQWLAPKQ 300
    GTDSALAMAM GHVILKEFHL DNPSDYFINY CRRYSDMPML VMLEPRDDGS 350
    YVPGRMIRAS DLVDGLGESN NPQWKTVAVN TAGELVVPNG SIGFRWGEKG 400
    KWNLESIAAG TETELSLTLL GQHDAVAGVA FPYFGGIENP HFRSVKHNPV 450
    LVRQLPVKNL TLVDGNTCPV VSVYDLVLAN YGLDRGLEDE NSAKDYAEIK 500
    PYTPAWGEQI TGVPRQYIET IAREFADTAH KTHGRSMIIL GAGVNHWYHM 550
    DMNYRGMINM LIFCGCVGQS GGGWAHYVGQ EKLRPQTGWL PLAFALDWNR 600
    PPRQMNSTSF FYNHSSQWRY EKVSAQELLS PLADASKYSG HLIDFNVRAE 650
    RMGWLPSAPQ LGRNPLGIKA EADKAGLSPT EFTAQALKSG DLRMACEQPD 700
    SSSNHPRNLF VWRSNLLGSS GKGHEYMQKY LLGTESGIQG EELGASDGIK 750
    PEEVEWQTAA IEGKLDLLVT LDFRMSSTCL FSDIVLPTAT WYEKDDMNTS 800
    DMHPFIHPLS AAVDPAWESR SDWEIYKGIA KAFSQVCVGH LGKETDVVLQ 850
    PLLHDSPAEL SQPCEVLDWR KGECDLIPGK TAPNIVAVER DYPATYERFT 900
    SLGPLMDKLG NGGKGISWNT QDEIDFLGKL NYTKRDGPAQ GRPLIDTAID 950
    ASEVILALAP ETNGHVAVKA WQALGEITGR EHTHLALHKE DEKIRFRDIQ 1000
    AQPRKIISSP TWSGLESDHV SYNAGYTNVH ELIPWRTLSG RQQLYQDHPW 1050
    MRAFGESLVA YRPPIDTRSV SEMRQIPPNG FPEKALNFLT PHQKWGIHST 1100
    YSENLLMLTL SRGGPIVWIS ETDARELTIV DNDWVEVFNA NGALTARAVV 1150
    SQRVPPGMTM MYHAQERIMN IPGSEVTGMR GGIHNSVTRV CPKPTHMIGG 1200
    YAQLAWGFNY YGTVGSNRDE FIMIRKMKNV NWLDDEGRDQ VQEAKK 1246
    Length:1,246
    Mass (Da):140,227
    Last modified:January 23, 2007 - v5
    Checksum:iDB37433A9AB0E16E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1103 – 11031E → D in CAA34964. (PubMed:2233673)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17110 Genomic DNA. Translation: CAA34964.1.
    U00096 Genomic DNA. Translation: AAC74550.1.
    AP009048 Genomic DNA. Translation: BAA15105.1.
    X94992 Genomic DNA. Translation: CAA64449.1.
    PIRiG64899.
    RefSeqiNP_415985.1. NC_000913.3.
    YP_489733.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74550; AAC74550; b1468.
    BAA15105; BAA15105; BAA15105.
    GeneIDi12933903.
    945999.
    KEGGiecj:Y75_p1444.
    eco:b1468.
    PATRICi32118228. VBIEscCol129921_1534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17110 Genomic DNA. Translation: CAA34964.1 .
    U00096 Genomic DNA. Translation: AAC74550.1 .
    AP009048 Genomic DNA. Translation: BAA15105.1 .
    X94992 Genomic DNA. Translation: CAA64449.1 .
    PIRi G64899.
    RefSeqi NP_415985.1. NC_000913.3.
    YP_489733.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P19319.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10324N.
    IntActi P19319. 29 interactions.
    MINTi MINT-1261362.
    STRINGi 511145.b1468.

    Protein family/group databases

    TCDBi 5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

    Proteomic databases

    PaxDbi P19319.
    PRIDEi P19319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74550 ; AAC74550 ; b1468 .
    BAA15105 ; BAA15105 ; BAA15105 .
    GeneIDi 12933903.
    945999.
    KEGGi ecj:Y75_p1444.
    eco:b1468.
    PATRICi 32118228. VBIEscCol129921_1534.

    Organism-specific databases

    EchoBASEi EB0642.
    EcoGenei EG10648. narZ.

    Phylogenomic databases

    eggNOGi COG5013.
    HOGENOMi HOG000237341.
    KOi K00370.
    OMAi VKQEPVL.
    OrthoDBi EOG6RC3JS.
    PhylomeDBi P19319.

    Enzyme and pathway databases

    BioCyci EcoCyc:NARZ-MONOMER.
    ECOL316407:JW1463-MONOMER.
    MetaCyc:NARZ-MONOMER.

    Miscellaneous databases

    PROi P19319.

    Gene expression databases

    Genevestigatori P19319.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR006468. NarG.
    IPR028189. Nitr_red_alph_N.
    [Graphical view ]
    Pfami PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF14710. Nitr_red_alph_N. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01580. narG. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon."
      Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.
      Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. Blasco F.
      Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. Bonnefoy V., Ratouchniak J., Blasco F., Chippaux M.
      Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
      Strain: K12.

    Entry informationi

    Entry nameiNARZ_ECOLI
    AccessioniPrimary (citable) accession number: P19319
    Secondary accession number(s): P78063
    , P78154, P78155, P78156
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 137 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3