Respiratory nitrate reductase 2 alpha chain - Escherichia coli (strain K12)

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P19319 (NARZ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Respiratory nitrate reductase 2 alpha chain
EC=1.7.99.4

Gene names

Name:	narZ
Ordered Locus Names:	b1468, JW1463

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	1246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction.
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Tetramer composed of an alpha, a beta and 2 gamma chains. Alpha and beta are catalytic chains; gamma chain is involved in binding the enzyme complex to the cytoplasmic membrane.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Cell membrane Membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	anaerobic respiration Inferred from direct assay PubMed 4869216. Source: EcoCyc electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	membrane Inferred from direct assay PubMed 16858726. Source: UniProtKB nitrate reductase complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from physical interaction PubMed 1321049. Source: EcoCyc electron carrier activity Inferred from direct assay PubMed 2139607. Source: EcoCyc iron ion binding Inferred from physical interaction PubMed 1321049. Source: EcoCyc molybdenum ion binding Inferred from direct assay PubMed 2139607. Source: EcoCyc nitrate reductase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on NAD(P)H Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
narH	P11349	3	EBI-547262,EBI-555067
narJ	P0AF26	3	EBI-547262,EBI-555043

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 1246

1245

Respiratory nitrate reductase 2 alpha chain

PRO_0000063234

Sites

Metal binding

Iron-sulfur (4Fe-4S); via pros nitrogen By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

Iron-sulfur (4Fe-4S) By similarity

Metal binding

223

Molybdenum By similarity

Experimental info

Sequence conflict

1103

E → D in CAA34964. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P19319 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: DB37433A9AB0E16E
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FASTA

1,246

140,227

        10         20         30         40         50         60 
MSKLLDRFRY FKQKGETFAD GHGQVMHSNR DWEDSYRQRW QFDKIVRSTH GVNCTGSCSW 

        70         80         90        100        110        120 
KIYVKNGLVT WEIQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPLIRKRLIE 

       130        140        150        160        170        180 
LWREALKQHS DPVLAWASIM NDPQKCLSYK QVRGRGGFIR SNWQELNQLI AAANVWTIKT 

       190        200        210        220        230        240 
YGPDRVAGFS PIPAMSMVSY AAGTRYLSLL GGTCLSFYDW YCDLPPASPM TWGEQTDVPE 

       250        260        270        280        290        300 
SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTIA ITPDYSEVAK LCDQWLAPKQ 

       310        320        330        340        350        360 
GTDSALAMAM GHVILKEFHL DNPSDYFINY CRRYSDMPML VMLEPRDDGS YVPGRMIRAS 

       370        380        390        400        410        420 
DLVDGLGESN NPQWKTVAVN TAGELVVPNG SIGFRWGEKG KWNLESIAAG TETELSLTLL 

       430        440        450        460        470        480 
GQHDAVAGVA FPYFGGIENP HFRSVKHNPV LVRQLPVKNL TLVDGNTCPV VSVYDLVLAN 

       490        500        510        520        530        540 
YGLDRGLEDE NSAKDYAEIK PYTPAWGEQI TGVPRQYIET IAREFADTAH KTHGRSMIIL 

       550        560        570        580        590        600 
GAGVNHWYHM DMNYRGMINM LIFCGCVGQS GGGWAHYVGQ EKLRPQTGWL PLAFALDWNR 

       610        620        630        640        650        660 
PPRQMNSTSF FYNHSSQWRY EKVSAQELLS PLADASKYSG HLIDFNVRAE RMGWLPSAPQ 

       670        680        690        700        710        720 
LGRNPLGIKA EADKAGLSPT EFTAQALKSG DLRMACEQPD SSSNHPRNLF VWRSNLLGSS 

       730        740        750        760        770        780 
GKGHEYMQKY LLGTESGIQG EELGASDGIK PEEVEWQTAA IEGKLDLLVT LDFRMSSTCL 

       790        800        810        820        830        840 
FSDIVLPTAT WYEKDDMNTS DMHPFIHPLS AAVDPAWESR SDWEIYKGIA KAFSQVCVGH 

       850        860        870        880        890        900 
LGKETDVVLQ PLLHDSPAEL SQPCEVLDWR KGECDLIPGK TAPNIVAVER DYPATYERFT 

       910        920        930        940        950        960 
SLGPLMDKLG NGGKGISWNT QDEIDFLGKL NYTKRDGPAQ GRPLIDTAID ASEVILALAP 

       970        980        990       1000       1010       1020 
ETNGHVAVKA WQALGEITGR EHTHLALHKE DEKIRFRDIQ AQPRKIISSP TWSGLESDHV 

      1030       1040       1050       1060       1070       1080 
SYNAGYTNVH ELIPWRTLSG RQQLYQDHPW MRAFGESLVA YRPPIDTRSV SEMRQIPPNG 

      1090       1100       1110       1120       1130       1140 
FPEKALNFLT PHQKWGIHST YSENLLMLTL SRGGPIVWIS ETDARELTIV DNDWVEVFNA 

      1150       1160       1170       1180       1190       1200 
NGALTARAVV SQRVPPGMTM MYHAQERIMN IPGSEVTGMR GGIHNSVTRV CPKPTHMIGG 

      1210       1220       1230       1240 
YAQLAWGFNY YGTVGSNRDE FIMIRKMKNV NWLDDEGRDQ VQEAKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon." Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M. Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	Blasco F. Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	Bonnefoy V., Ratouchniak J., Blasco F., Chippaux M. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17110 Genomic DNA. Translation: CAA34964.1. U00096 Genomic DNA. Translation: AAC74550.1. AP009048 Genomic DNA. Translation: BAA15105.1. X94992 Genomic DNA. Translation: CAA64449.1.
PIR	G64899.
RefSeq	NP_415985.1. NC_000913.2. YP_489733.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P19319.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10324N.
IntAct	P19319. 28 interactions.
MINT	MINT-1261362.
STRING	511145.b1468.
Protein family/group databases
TCDB	5.A.3.1.2. prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
Proteomic databases
PaxDb	P19319.
PRIDE	P19319.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74550; AAC74550; b1468. BAA15105; BAA15105; BAA15105.
GeneID	12933903. 945999.
KEGG	ecj:Y75_p1444. eco:b1468.
PATRIC	32118228. VBIEscCol129921_1534.
Organism-specific databases
EchoBASE	EB0642.
EcoGene	EG10648. narZ.
Phylogenomic databases
eggNOG	COG5013.
HOGENOM	HOG000237341.
KO	K08345.
OMA	RSQEVVY.
ProtClustDB	CLSK868288.
Enzyme and pathway databases
BioCyc	EcoCyc:NARZ-MONOMER. ECOL316407:JW1463-MONOMER. MetaCyc:NARZ-MONOMER.
Gene expression databases
Genevestigator	P19319.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006468. NarG. [Graphical view]
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01580. narG. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NARZ_ECOLI

Accession

Primary (citable) accession number: P19319
Secondary accession number(s): P78063

P78156

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 125 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents