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P19319 (NARZ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Respiratory nitrate reductase 2 alpha chain

EC=1.7.99.4
Gene names
Name:narZ
Ordered Locus Names:b1468, JW1463
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length1246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth.

The alpha chain is the actual site of nitrate reduction.

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Tetramer composed of an alpha, a beta and 2 gamma chains. Alpha and beta are catalytic chains; gamma chain is involved in binding the enzyme complex to the cytoplasmic membrane.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 12461245Respiratory nitrate reductase 2 alpha chain
PRO_0000063234

Regions

Domain43 – 107654Fe-4S Mo/W bis-MGD-type

Sites

Metal binding501Iron-sulfur (4Fe-4S); via pros nitrogen By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding931Iron-sulfur (4Fe-4S) By similarity
Metal binding2231Molybdenum By similarity

Experimental info

Sequence conflict11031E → D in CAA34964. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19319 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: DB37433A9AB0E16E

FASTA1,246140,227
        10         20         30         40         50         60 
MSKLLDRFRY FKQKGETFAD GHGQVMHSNR DWEDSYRQRW QFDKIVRSTH GVNCTGSCSW 

        70         80         90        100        110        120 
KIYVKNGLVT WEIQQTDYPR TRPDLPNHEP RGCPRGASYS WYLYSANRLK YPLIRKRLIE 

       130        140        150        160        170        180 
LWREALKQHS DPVLAWASIM NDPQKCLSYK QVRGRGGFIR SNWQELNQLI AAANVWTIKT 

       190        200        210        220        230        240 
YGPDRVAGFS PIPAMSMVSY AAGTRYLSLL GGTCLSFYDW YCDLPPASPM TWGEQTDVPE 

       250        260        270        280        290        300 
SADWYNSSYI IAWGSNVPQT RTPDAHFFTE VRYKGTKTIA ITPDYSEVAK LCDQWLAPKQ 

       310        320        330        340        350        360 
GTDSALAMAM GHVILKEFHL DNPSDYFINY CRRYSDMPML VMLEPRDDGS YVPGRMIRAS 

       370        380        390        400        410        420 
DLVDGLGESN NPQWKTVAVN TAGELVVPNG SIGFRWGEKG KWNLESIAAG TETELSLTLL 

       430        440        450        460        470        480 
GQHDAVAGVA FPYFGGIENP HFRSVKHNPV LVRQLPVKNL TLVDGNTCPV VSVYDLVLAN 

       490        500        510        520        530        540 
YGLDRGLEDE NSAKDYAEIK PYTPAWGEQI TGVPRQYIET IAREFADTAH KTHGRSMIIL 

       550        560        570        580        590        600 
GAGVNHWYHM DMNYRGMINM LIFCGCVGQS GGGWAHYVGQ EKLRPQTGWL PLAFALDWNR 

       610        620        630        640        650        660 
PPRQMNSTSF FYNHSSQWRY EKVSAQELLS PLADASKYSG HLIDFNVRAE RMGWLPSAPQ 

       670        680        690        700        710        720 
LGRNPLGIKA EADKAGLSPT EFTAQALKSG DLRMACEQPD SSSNHPRNLF VWRSNLLGSS 

       730        740        750        760        770        780 
GKGHEYMQKY LLGTESGIQG EELGASDGIK PEEVEWQTAA IEGKLDLLVT LDFRMSSTCL 

       790        800        810        820        830        840 
FSDIVLPTAT WYEKDDMNTS DMHPFIHPLS AAVDPAWESR SDWEIYKGIA KAFSQVCVGH 

       850        860        870        880        890        900 
LGKETDVVLQ PLLHDSPAEL SQPCEVLDWR KGECDLIPGK TAPNIVAVER DYPATYERFT 

       910        920        930        940        950        960 
SLGPLMDKLG NGGKGISWNT QDEIDFLGKL NYTKRDGPAQ GRPLIDTAID ASEVILALAP 

       970        980        990       1000       1010       1020 
ETNGHVAVKA WQALGEITGR EHTHLALHKE DEKIRFRDIQ AQPRKIISSP TWSGLESDHV 

      1030       1040       1050       1060       1070       1080 
SYNAGYTNVH ELIPWRTLSG RQQLYQDHPW MRAFGESLVA YRPPIDTRSV SEMRQIPPNG 

      1090       1100       1110       1120       1130       1140 
FPEKALNFLT PHQKWGIHST YSENLLMLTL SRGGPIVWIS ETDARELTIV DNDWVEVFNA 

      1150       1160       1170       1180       1190       1200 
NGALTARAVV SQRVPPGMTM MYHAQERIMN IPGSEVTGMR GGIHNSVTRV CPKPTHMIGG 

      1210       1220       1230       1240 
YAQLAWGFNY YGTVGSNRDE FIMIRKMKNV NWLDDEGRDQ VQEAKK 

« Hide

References

« Hide 'large scale' references
[1]"Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon."
Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.
Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]Blasco F.
Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]Bonnefoy V., Ratouchniak J., Blasco F., Chippaux M.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17110 Genomic DNA. Translation: CAA34964.1.
U00096 Genomic DNA. Translation: AAC74550.1.
AP009048 Genomic DNA. Translation: BAA15105.1.
X94992 Genomic DNA. Translation: CAA64449.1.
PIRG64899.
RefSeqNP_415985.1. NC_000913.3.
YP_489733.1. NC_007779.1.

3D structure databases

ProteinModelPortalP19319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10324N.
IntActP19319. 29 interactions.
MINTMINT-1261362.
STRING511145.b1468.

Protein family/group databases

TCDB5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PaxDbP19319.
PRIDEP19319.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74550; AAC74550; b1468.
BAA15105; BAA15105; BAA15105.
GeneID12933903.
945999.
KEGGecj:Y75_p1444.
eco:b1468.
PATRIC32118228. VBIEscCol129921_1534.

Organism-specific databases

EchoBASEEB0642.
EcoGeneEG10648. narZ.

Phylogenomic databases

eggNOGCOG5013.
HOGENOMHOG000237341.
KOK00370.
OMAVKQEPVL.
OrthoDBEOG6RC3JS.
PhylomeDBP19319.

Enzyme and pathway databases

BioCycEcoCyc:NARZ-MONOMER.
ECOL316407:JW1463-MONOMER.
MetaCyc:NARZ-MONOMER.

Gene expression databases

GenevestigatorP19319.

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01580. narG. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP19319.

Entry information

Entry nameNARZ_ECOLI
AccessionPrimary (citable) accession number: P19319
Secondary accession number(s): P78063 expand/collapse secondary AC list , P78154, P78155, P78156
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 136 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene