Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Respiratory nitrate reductase 2 alpha chain

Gene

narZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth.
The alpha chain is the actual site of nitrate reduction.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi50 – 501Iron-sulfur (4Fe-4S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi54 – 541Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi58 – 581Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi93 – 931Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi223 – 2231MolybdenumBy similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • electron carrier activity Source: EcoCyc
  • iron-sulfur cluster binding Source: GO_Central
  • molybdenum ion binding Source: EcoCyc
  • NADH dehydrogenase activity Source: GO_Central
  • nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciEcoCyc:NARZ-MONOMER.
ECOL316407:JW1463-MONOMER.
MetaCyc:NARZ-MONOMER.

Protein family/group databases

TCDBi5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory nitrate reductase 2 alpha chain (EC:1.7.99.4)
Gene namesi
Name:narZ
Ordered Locus Names:b1468, JW1463
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10648. narZ.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • nitrate reductase complex Source: InterPro
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 12461245Respiratory nitrate reductase 2 alpha chainPRO_0000063234Add
BLAST

Proteomic databases

PaxDbiP19319.
PRIDEiP19319.

Interactioni

Subunit structurei

Tetramer composed of an alpha, a beta and 2 gamma chains. Alpha and beta are catalytic chains; gamma chain is involved in binding the enzyme complex to the cytoplasmic membrane.

Binary interactionsi

WithEntry#Exp.IntActNotes
narHP113494EBI-547262,EBI-555067
narJP0AF263EBI-547262,EBI-555043
narWP193173EBI-547262,EBI-555088

Protein-protein interaction databases

DIPiDIP-10324N.
IntActiP19319. 29 interactions.
MINTiMINT-1261362.
STRINGi511145.b1468.

Structurei

3D structure databases

ProteinModelPortaliP19319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 107654Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5013.
HOGENOMiHOG000237341.
InParanoidiP19319.
KOiK00370.
OMAiVKQEPVL.
OrthoDBiEOG6RC3JS.
PhylomeDBiP19319.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01580. narG. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLLDRFRY FKQKGETFAD GHGQVMHSNR DWEDSYRQRW QFDKIVRSTH
60 70 80 90 100
GVNCTGSCSW KIYVKNGLVT WEIQQTDYPR TRPDLPNHEP RGCPRGASYS
110 120 130 140 150
WYLYSANRLK YPLIRKRLIE LWREALKQHS DPVLAWASIM NDPQKCLSYK
160 170 180 190 200
QVRGRGGFIR SNWQELNQLI AAANVWTIKT YGPDRVAGFS PIPAMSMVSY
210 220 230 240 250
AAGTRYLSLL GGTCLSFYDW YCDLPPASPM TWGEQTDVPE SADWYNSSYI
260 270 280 290 300
IAWGSNVPQT RTPDAHFFTE VRYKGTKTIA ITPDYSEVAK LCDQWLAPKQ
310 320 330 340 350
GTDSALAMAM GHVILKEFHL DNPSDYFINY CRRYSDMPML VMLEPRDDGS
360 370 380 390 400
YVPGRMIRAS DLVDGLGESN NPQWKTVAVN TAGELVVPNG SIGFRWGEKG
410 420 430 440 450
KWNLESIAAG TETELSLTLL GQHDAVAGVA FPYFGGIENP HFRSVKHNPV
460 470 480 490 500
LVRQLPVKNL TLVDGNTCPV VSVYDLVLAN YGLDRGLEDE NSAKDYAEIK
510 520 530 540 550
PYTPAWGEQI TGVPRQYIET IAREFADTAH KTHGRSMIIL GAGVNHWYHM
560 570 580 590 600
DMNYRGMINM LIFCGCVGQS GGGWAHYVGQ EKLRPQTGWL PLAFALDWNR
610 620 630 640 650
PPRQMNSTSF FYNHSSQWRY EKVSAQELLS PLADASKYSG HLIDFNVRAE
660 670 680 690 700
RMGWLPSAPQ LGRNPLGIKA EADKAGLSPT EFTAQALKSG DLRMACEQPD
710 720 730 740 750
SSSNHPRNLF VWRSNLLGSS GKGHEYMQKY LLGTESGIQG EELGASDGIK
760 770 780 790 800
PEEVEWQTAA IEGKLDLLVT LDFRMSSTCL FSDIVLPTAT WYEKDDMNTS
810 820 830 840 850
DMHPFIHPLS AAVDPAWESR SDWEIYKGIA KAFSQVCVGH LGKETDVVLQ
860 870 880 890 900
PLLHDSPAEL SQPCEVLDWR KGECDLIPGK TAPNIVAVER DYPATYERFT
910 920 930 940 950
SLGPLMDKLG NGGKGISWNT QDEIDFLGKL NYTKRDGPAQ GRPLIDTAID
960 970 980 990 1000
ASEVILALAP ETNGHVAVKA WQALGEITGR EHTHLALHKE DEKIRFRDIQ
1010 1020 1030 1040 1050
AQPRKIISSP TWSGLESDHV SYNAGYTNVH ELIPWRTLSG RQQLYQDHPW
1060 1070 1080 1090 1100
MRAFGESLVA YRPPIDTRSV SEMRQIPPNG FPEKALNFLT PHQKWGIHST
1110 1120 1130 1140 1150
YSENLLMLTL SRGGPIVWIS ETDARELTIV DNDWVEVFNA NGALTARAVV
1160 1170 1180 1190 1200
SQRVPPGMTM MYHAQERIMN IPGSEVTGMR GGIHNSVTRV CPKPTHMIGG
1210 1220 1230 1240
YAQLAWGFNY YGTVGSNRDE FIMIRKMKNV NWLDDEGRDQ VQEAKK
Length:1,246
Mass (Da):140,227
Last modified:January 23, 2007 - v5
Checksum:iDB37433A9AB0E16E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1103 – 11031E → D in CAA34964 (PubMed:2233673).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17110 Genomic DNA. Translation: CAA34964.1.
U00096 Genomic DNA. Translation: AAC74550.1.
AP009048 Genomic DNA. Translation: BAA15105.1.
X94992 Genomic DNA. Translation: CAA64449.1.
PIRiG64899.
RefSeqiNP_415985.1. NC_000913.3.
WP_000040458.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC74550; AAC74550; b1468.
BAA15105; BAA15105; BAA15105.
GeneIDi945999.
KEGGieco:b1468.
PATRICi32118228. VBIEscCol129921_1534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17110 Genomic DNA. Translation: CAA34964.1.
U00096 Genomic DNA. Translation: AAC74550.1.
AP009048 Genomic DNA. Translation: BAA15105.1.
X94992 Genomic DNA. Translation: CAA64449.1.
PIRiG64899.
RefSeqiNP_415985.1. NC_000913.3.
WP_000040458.1. NZ_CP010445.1.

3D structure databases

ProteinModelPortaliP19319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10324N.
IntActiP19319. 29 interactions.
MINTiMINT-1261362.
STRINGi511145.b1468.

Protein family/group databases

TCDBi5.A.3.1.2. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Proteomic databases

PaxDbiP19319.
PRIDEiP19319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74550; AAC74550; b1468.
BAA15105; BAA15105; BAA15105.
GeneIDi945999.
KEGGieco:b1468.
PATRICi32118228. VBIEscCol129921_1534.

Organism-specific databases

EchoBASEiEB0642.
EcoGeneiEG10648. narZ.

Phylogenomic databases

eggNOGiCOG5013.
HOGENOMiHOG000237341.
InParanoidiP19319.
KOiK00370.
OMAiVKQEPVL.
OrthoDBiEOG6RC3JS.
PhylomeDBiP19319.

Enzyme and pathway databases

BioCyciEcoCyc:NARZ-MONOMER.
ECOL316407:JW1463-MONOMER.
MetaCyc:NARZ-MONOMER.

Miscellaneous databases

PROiP19319.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01580. narG. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon."
    Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.
    Mol. Gen. Genet. 222:104-111(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. Blasco F.
    Submitted (JUL-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Bonnefoy V., Ratouchniak J., Blasco F., Chippaux M.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
    Strain: K12.

Entry informationi

Entry nameiNARZ_ECOLI
AccessioniPrimary (citable) accession number: P19319
Secondary accession number(s): P78063
, P78154, P78155, P78156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 145 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.