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Protein

Cystatin-S

Gene

Cst4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Reactive site1

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: RGD
  • protease binding Source: RGD

GO - Biological processi

  • cell development Source: RGD
  • cell killing Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of cysteine-type endopeptidase activity Source: GO_Central
  • negative regulation of peptidase activity Source: RGD
  • response to antibiotic Source: RGD
  • response to axon injury Source: RGD
  • response to chromate Source: RGD
  • response to drug Source: RGD
  • response to hormone Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to protozoan Source: RGD
  • response to wounding Source: RGD
  • salivary gland development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-S
Alternative name(s):
Cystatin-1
Protein LM
Gene namesi
Name:Cst4
Synonyms:Cyss
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi735160. Cyss.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: RGD
  • secretory granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000000665128 – 141Cystatin-SAdd BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi94 ↔ 1041 Publication
Disulfide bondi118 ↔ 1381 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19313.
PRIDEiP19313.

Expressioni

Tissue specificityi

Found in saliva, tears, urine and seminal fluid.

Gene expression databases

GenevisibleiP19313. RN.

Interactioni

GO - Molecular functioni

  • protease binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044719.

Structurei

3D structure databases

ProteinModelPortaliP19313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 80Secondary area of contact5

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
GeneTreeiENSGT00860000133739.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP19313.
OMAiADSEPCA.
OrthoDBiEOG091G0TP1.
PhylomeDBiP19313.

Family and domain databases

CDDicd00042. CY. 1 hit.
InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYLLHAQLF LLTTFILVLN MRLCPVLGHF LGGIEKSSME EEGASEALNY
60 70 80 90 100
AVNEYNEKNS DLYLSRVVEV KDVQKQVVAG TKFFFDVILG KTICLKTQGD
110 120 130 140
LTNCPLNEEA DQQEHEFCSF VVHDIPWENY IVLLSSSCHS I
Length:141
Mass (Da):15,949
Last modified:February 1, 1996 - v2
Checksum:iD7632905541C8266
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114 – 115EH → QE AA sequence (PubMed:2757396).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75281 Genomic DNA. Translation: AAA41068.1.
J04206 mRNA. Translation: AAB59703.1.
PIRiJQ1470.
RefSeqiNP_941958.1. NM_198685.1.
UniGeneiRn.214033.

Genome annotation databases

EnsembliENSRNOT00000044345; ENSRNOP00000044719; ENSRNOG00000030857.
GeneIDi296234.
KEGGirno:296234.
UCSCiRGD:735160. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75281 Genomic DNA. Translation: AAA41068.1.
J04206 mRNA. Translation: AAB59703.1.
PIRiJQ1470.
RefSeqiNP_941958.1. NM_198685.1.
UniGeneiRn.214033.

3D structure databases

ProteinModelPortaliP19313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044719.

Proteomic databases

PaxDbiP19313.
PRIDEiP19313.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000044345; ENSRNOP00000044719; ENSRNOG00000030857.
GeneIDi296234.
KEGGirno:296234.
UCSCiRGD:735160. rat.

Organism-specific databases

CTDi296234.
RGDi735160. Cyss.

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
GeneTreeiENSGT00860000133739.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP19313.
OMAiADSEPCA.
OrthoDBiEOG091G0TP1.
PhylomeDBiP19313.

Miscellaneous databases

PROiP19313.

Gene expression databases

GenevisibleiP19313. RN.

Family and domain databases

CDDicd00042. CY. 1 hit.
InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYTS_RAT
AccessioniPrimary (citable) accession number: P19313
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.