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Protein

Cystatin-S

Gene

Cst4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei32Reactive site1

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: RGD
  • protease binding Source: RGD

GO - Biological processi

  • cell development Source: RGD
  • cell killing Source: RGD
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of cysteine-type endopeptidase activity Source: GO_Central
  • negative regulation of peptidase activity Source: RGD
  • response to antibiotic Source: RGD
  • response to axon injury Source: RGD
  • response to chromate Source: RGD
  • response to drug Source: RGD
  • response to hormone Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to protozoan Source: RGD
  • response to wounding Source: RGD
  • salivary gland development Source: RGD

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-S
Alternative name(s):
Cystatin-1
Protein LM
Gene namesi
Name:Cst4
Synonyms:Cyss
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi735160. Cyss.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: RGD
  • secretory granule Source: RGD

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
ChainiPRO_000000665128 – 141Cystatin-SAdd BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi94 ↔ 1041 Publication
Disulfide bondi118 ↔ 1381 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19313.
PRIDEiP19313.

Expressioni

Tissue specificityi

Found in saliva, tears, urine and seminal fluid.

Gene expression databases

GenevisibleiP19313. RN.

Interactioni

GO - Molecular functioni

  • protease binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044719.

Structurei

3D structure databases

ProteinModelPortaliP19313.
SMRiP19313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 80Secondary area of contact5

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
GeneTreeiENSGT00880000137924.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP19313.
OrthoDBiEOG091G0TP1.
PhylomeDBiP19313.

Family and domain databases

CDDicd00042. CY. 1 hit.
InterProiView protein in InterPro
IPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiView protein in Pfam
PF00031. Cystatin. 1 hit.
SMARTiView protein in SMART
SM00043. CY. 1 hit.
PROSITEiView protein in PROSITE
PS00287. CYSTATIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19313-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYLLHAQLF LLTTFILVLN MRLCPVLGHF LGGIEKSSME EEGASEALNY
60 70 80 90 100
AVNEYNEKNS DLYLSRVVEV KDVQKQVVAG TKFFFDVILG KTICLKTQGD
110 120 130 140
LTNCPLNEEA DQQEHEFCSF VVHDIPWENY IVLLSSSCHS I
Length:141
Mass (Da):15,949
Last modified:February 1, 1996 - v2
Checksum:iD7632905541C8266
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114 – 115EH → QE AA sequence (PubMed:2757396).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75281 Genomic DNA. Translation: AAA41068.1.
J04206 mRNA. Translation: AAB59703.1.
PIRiJQ1470.
RefSeqiNP_941958.1. NM_198685.1.
UniGeneiRn.214033.

Genome annotation databases

EnsembliENSRNOT00000044345; ENSRNOP00000044719; ENSRNOG00000030857.
GeneIDi296234.
KEGGirno:296234.
UCSCiRGD:735160. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCYTS_RAT
AccessioniPrimary (citable) accession number: P19313
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1996
Last modified: July 5, 2017
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families