Reviewed,
UniProtKB/Swiss-Prot P19269 (AMY1_DEBOC)
Last modified
January 19, 2010.
Version 73.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alpha-amylase 1 EC=3.2.1.1 Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase 1 | ||
| Gene names |
| ||
| Organism | Debaryomyces occidentalis (Yeast) (Schwanniomyces occidentalis) | ||
| Taxonomic identifier | 27300 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Debaryomyces |
Protein attributes
| Sequence length | 512 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Catalytic activity | Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides. |
| Cofactor | Binds 2 calcium ions per subunit. Calcium is inhibitory at high concentrations By similarity. |
| Enzyme regulation | Alpha-amylase expression underlies catabolite repression by glucose. |
| Sequence similarities | Belongs to the glycosyl hydrolase 13 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism |
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | carbohydrate catabolic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | alpha-amylase activity Inferred from electronic annotation. Source: EC calcium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 25 | 25 | Potential | ||||||||
| Chain | 26 – 512 | 487 | Alpha-amylase 1 | PRO_0000001352 | |||||||
Sites | |||||||||||
| Active site | 242 | 1 | Nucleophile By similarity | ||||||||
| Active site | 266 | 1 | Proton donor By similarity | ||||||||
| Active site | 333 | 1 | By similarity | ||||||||
| Metal binding | 157 | 1 | Calcium 1 By similarity | ||||||||
| Metal binding | 198 | 1 | Calcium 1; via carbonyl oxygen By similarity | ||||||||
| Metal binding | 211 | 1 | Calcium 1 By similarity | ||||||||
| Metal binding | 242 | 1 | Calcium 2 By similarity | ||||||||
| Metal binding | 246 | 1 | Calcium 1; via carbonyl oxygen By similarity | ||||||||
| Metal binding | 266 | 1 | Calcium 2 By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 233 | 1 | N-linked (GlcNAc...) Probable | ||||||||
| Disulfide bond | 66 ↔ 74 | By similarity | |||||||||
| Disulfide bond | 186 ↔ 200 | By similarity | |||||||||
| Disulfide bond | 276 ↔ 319 | By similarity | |||||||||
| Disulfide bond | 475 ↔ 510 | By similarity | |||||||||
Natural variations | |||||||||||
| Natural variant | 32 | 1 | M → K in strain: CCRC 21164 and ATCC 26077 / CBS 2863. | ||||||||
| Natural variant | 36 | 1 | S → G in strain: CCRC 21164. | ||||||||
| Natural variant | 73 | 1 | Y → I in strain: ATCC 26077 / CBS 2863. | ||||||||
| Natural variant | 280 | 1 | N → S in strain: CCRC 21164. | ||||||||
| Natural variant | 350 | 1 | D → A in strain: CCRC 21164 and ATCC 26077 / CBS 2863. | ||||||||
| Natural variant | 479 | 1 | L → S in strain: CCRC 21164 and ATCC 26077 / CBS 2863. | ||||||||
| Natural variant | 483 | 1 | S → F in strain: CCRC 21164. | ||||||||
Sequences
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References
| [1] | "Analysis of the alpha-amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera." Strasser A.W.M., Selk R., Dohmen R.J., Niermann T., Bielefeld M., Seeboth P., Tu G., Hollenberg C.P. Eur. J. Biochem. 184:699-706(1989) [PubMed: 2806251] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26076 / CBS 2864 / CCRC 22059 / NRRL Y-2470. |
| [2] | "The nucleotide sequence of Schwanniomyces occidentalis alpha-amylase gene." Wu F.M., Wang T.T., Hsu W.H. FEMS Microbiol. Lett. 66:313-318(1991) [PubMed: 1769525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CCRC 21164. |
| [3] | "Nucleotide sequence of the extracellular alpha-amylase gene in the yeast Schwanniomyces occidentalis ATCC 26077." Park J.C., Bai S., Tai C.Y., Chun S.B. FEMS Microbiol. Lett. 72:17-23(1992) [PubMed: 1612414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26077 / CBS 2863 / IFO 1840 / JCM 8124 / NRRL Y-2477. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | S77586 Genomic DNA. Translation: AAB21151.2. X16040 Genomic DNA. Translation: CAA34162.1. X62079 Genomic DNA. Translation: CAA43995.1. S38381 Genomic DNA. Translation: AAB22383.2. |
| PIR | S06115. S23355. |
3D structure databases | |
| SMR | P19269. Positions 39-511. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH13. Glycoside Hydrolase Family 13. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.1. 290444. |
Family and domain databases | |
| InterPro | IPR013777. A-amylase_fun. IPR015340. A_amylase_DUF1966_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| Pfam | PF00128. Alpha-amylase. 1 hit. PF09260. DUF1966. 1 hit. [Graphical view] |
| PIRSF | PIRSF001024. Alph-amyl_fung. 1 hit. |
| SMART | SM00642. Aamy. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AMY1_DEBOC | ||||||||
| Accession | Primary (citable) accession number: P19269 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


