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Protein

60S ribosomal protein L13a

Gene

Rpl13a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex.By similarity1 Publication

GO - Molecular functioni

  • mRNA binding Source: MGI
  • poly(A) RNA binding Source: MGI
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

  • cellular response to interferon-gamma Source: UniProtKB
  • lung morphogenesis Source: MGI
  • macrophage chemotaxis Source: MGI
  • negative regulation of formation of translation preinitiation complex Source: MGI
  • negative regulation of translation Source: UniProtKB
  • regulation of homeostatic process Source: MGI
  • response to lipopolysaccharide Source: MGI
  • translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein, Tumor antigen

Keywords - Biological processi

Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-MMU-6791226. Major pathway of rRNA processing in the nucleolus.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-MMU-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L13a
Alternative name(s):
Transplantation antigen P198
Tum-P198 antigen
Gene namesi
Name:Rpl13a
Synonyms:P198, Tstap198-7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1351455. Rpl13a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosolic large ribosomal subunit Source: MGI
  • focal adhesion Source: MGI
  • GAIT complex Source: UniProtKB
  • intracellular ribonucleoprotein complex Source: MGI
  • membrane Source: MGI
  • nucleolus Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771S → A: Loss of interferon-gamma induced phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 20320260S ribosomal protein L13aPRO_0000133770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei59 – 591Citrulline1 Publication
Modified residuei77 – 771Phosphoserine; by ZIPK/DAPK31 Publication
Modified residuei140 – 1401Citrulline1 Publication
Modified residuei191 – 1911N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylation at Ser-77 upon interferon-gamma treatment in macrophages involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.1 Publication
Citrullinated by PADI4.1 Publication

Keywords - PTMi

Acetylation, Citrullination, Phosphoprotein

Proteomic databases

EPDiP19253.
PaxDbiP19253.
PeptideAtlasiP19253.
PRIDEiP19253.

PTM databases

iPTMnetiP19253.
PhosphoSiteiP19253.
SwissPalmiP19253.

Expressioni

Gene expression databases

BgeeiENSMUSG00000074129.
CleanExiMM_RPL13A.
ExpressionAtlasiP19253. baseline and differential.
GenevisibleiP19253. MM.

Interactioni

Subunit structurei

Component of the 60S ribosome. Component of the GAIT complex. Interacts with EIF4G1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi204356. 4 interactions.
IntActiP19253. 6 interactions.
MINTiMINT-1858287.
STRINGi10090.ENSMUSP00000115722.

Structurei

3D structure databases

ProteinModelPortaliP19253.
SMRiP19253. Positions 7-146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L13P family.Curated

Phylogenomic databases

eggNOGiKOG3204. Eukaryota.
COG0102. LUCA.
HOGENOMiHOG000225289.
HOVERGENiHBG062176.
InParanoidiP19253.
KOiK02872.
OMAiYYERKKV.
TreeFamiTF300159.

Family and domain databases

CDDicd00392. Ribosomal_L13. 1 hit.
Gene3Di3.90.1180.10. 1 hit.
HAMAPiMF_01366. Ribosomal_L13. 1 hit.
InterProiIPR005822. Ribosomal_L13.
IPR023563. Ribosomal_L13_CS.
IPR005755. Ribosomal_L13_euk/arc.
[Graphical view]
PANTHERiPTHR11545. PTHR11545. 1 hit.
PTHR11545:SF3. PTHR11545:SF3. 1 hit.
PfamiPF00572. Ribosomal_L13. 1 hit.
[Graphical view]
SUPFAMiSSF52161. SSF52161. 1 hit.
TIGRFAMsiTIGR01077. L13_A_E. 1 hit.
PROSITEiPS00783. RIBOSOMAL_L13. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN
60 70 80 90 100
KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLPH KTKRGQAALE
110 120 130 140 150
RLKVLDGIPP PYDKKKRMVV PAALKVVRLK PTRKFAYLGR LAHEVGWKYQ
160 170 180 190 200
AVTATLEEKR KEKAKMHYRK KKQILRLRKQ AEKNVEKKIC KFTEVLKTNG

LLV
Length:203
Mass (Da):23,464
Last modified:January 23, 2007 - v4
Checksum:i967AEAE3362E02CC
GO

Polymorphismi

The antigenic allele of P198 differs from the normal allele by a single mutation. The TUM- mutation P198 generates a new epitope recognized by syngeneic T-cells.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti154 – 1541A → T in allele TUM-.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51528 Genomic DNA. Translation: CAA35908.1.
BC082289 mRNA. Translation: AAH82289.1.
CCDSiCCDS21230.1.
PIRiJL0149.
RefSeqiNP_033464.2. NM_009438.5.
UniGeneiMm.180458.

Genome annotation databases

EnsembliENSMUST00000150350; ENSMUSP00000115722; ENSMUSG00000074129.
GeneIDi22121.
KEGGimmu:22121.
UCSCiuc009gtj.2. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51528 Genomic DNA. Translation: CAA35908.1.
BC082289 mRNA. Translation: AAH82289.1.
CCDSiCCDS21230.1.
PIRiJL0149.
RefSeqiNP_033464.2. NM_009438.5.
UniGeneiMm.180458.

3D structure databases

ProteinModelPortaliP19253.
SMRiP19253. Positions 7-146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204356. 4 interactions.
IntActiP19253. 6 interactions.
MINTiMINT-1858287.
STRINGi10090.ENSMUSP00000115722.

PTM databases

iPTMnetiP19253.
PhosphoSiteiP19253.
SwissPalmiP19253.

Proteomic databases

EPDiP19253.
PaxDbiP19253.
PeptideAtlasiP19253.
PRIDEiP19253.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000150350; ENSMUSP00000115722; ENSMUSG00000074129.
GeneIDi22121.
KEGGimmu:22121.
UCSCiuc009gtj.2. mouse.

Organism-specific databases

CTDi23521.
MGIiMGI:1351455. Rpl13a.

Phylogenomic databases

eggNOGiKOG3204. Eukaryota.
COG0102. LUCA.
HOGENOMiHOG000225289.
HOVERGENiHBG062176.
InParanoidiP19253.
KOiK02872.
OMAiYYERKKV.
TreeFamiTF300159.

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-MMU-6791226. Major pathway of rRNA processing in the nucleolus.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-MMU-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-MMU-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP19253.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000074129.
CleanExiMM_RPL13A.
ExpressionAtlasiP19253. baseline and differential.
GenevisibleiP19253. MM.

Family and domain databases

CDDicd00392. Ribosomal_L13. 1 hit.
Gene3Di3.90.1180.10. 1 hit.
HAMAPiMF_01366. Ribosomal_L13. 1 hit.
InterProiIPR005822. Ribosomal_L13.
IPR023563. Ribosomal_L13_CS.
IPR005755. Ribosomal_L13_euk/arc.
[Graphical view]
PANTHERiPTHR11545. PTHR11545. 1 hit.
PTHR11545:SF3. PTHR11545:SF3. 1 hit.
PfamiPF00572. Ribosomal_L13. 1 hit.
[Graphical view]
SUPFAMiSSF52161. SSF52161. 1 hit.
TIGRFAMsiTIGR01077. L13_A_E. 1 hit.
PROSITEiPS00783. RIBOSOMAL_L13. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRL13A_MOUSE
AccessioniPrimary (citable) accession number: P19253
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 153 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.