Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P19252 (ASNS2_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase, root [glutamine-hydrolyzing]

EC=6.3.5.4
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene names
Name:AS2
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Tissue specificity

Roots.

Induction

By darkness.

Sequence similarities

Contains 1 asparagine synthetase domain.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 583582Asparagine synthetase, root [glutamine-hydrolyzing]
PRO_0000056927

Regions

Domain2 – 185184Glutamine amidotransferase type-2
Domain237 – 516280Asparagine synthetase
Nucleotide binding341 – 3422ATP By similarity
Region50 – 545Glutamine binding By similarity
Region75 – 773Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site981Glutamine By similarity
Binding site2311ATP; via carbonyl oxygen By similarity
Binding site2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Site3431Important for beta-aspartyl-AMP intermediate formation By similarity

Experimental info

Sequence conflict341E → D in CAA73763. Ref.2
Sequence conflict451Y → F in CAA73763. Ref.2
Sequence conflict481Q → H in CAA73763. Ref.2
Sequence conflict781I → L in CAA73763. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P19252 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: E2C2DA6FEF1C72F1

FASTA58365,650
        10         20         30         40         50         60 
MCGILAVLGC SDPSRAKRVR VLELSRRLKH RGPEWSGLHQ HGDCYLAQQR LAIVDPASGD 

        70         80         90        100        110        120 
QPLFNEDNPS IVTVNGEIYN HEDLRKQLSN HTFRTGSDCD VIAHLYEEYG EDFVDMLDGI 

       130        140        150        160        170        180 
FSFVPLDTRD NSYIVARDAI GVTSLYIGWG LDGSVWISSE MKGLNDDCEH FECFPPGHLY 

       190        200        210        220        230        240 
SSKDSGFRRW YNPSWYSEAI PSAPYDPLAL RHAFEKAVVK RLMTDVPFGV LLSGGLDSSL 

       250        260        270        280        290        300 
VASITSRYLA TTKAAEQWGS KLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFTFTVQDGI 

       310        320        330        340        350        360 
DAIEDVIYHV ETYDVTSIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG GYLYFHKAPN 

       370        380        390        400        410        420 
KEEFHEETCR KIKALHQYDC QRANKSTYAW GLEARVPFLD KAFINVAMNI DPENKMIKRD 

       430        440        450        460        470        480 
EGRIEKYILR KAFDDEENPY LPKHILYRQK EQFSDGVGYS WIDGLKAHAA KHVTDKMMLN 

       490        500        510        520        530        540 
AGNIFPHNTP NTKEAYYYRM IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD 

       550        560        570        580 
PSGRAALGVH DSAYENHNKV NKTVEFEKII PLEAAPVELA IQG 

« Hide

References

[1]"Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum."
Tsai F.Y., Coruzzi G.M.
EMBO J. 9:323-332(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Sparkle.
Tissue: Root.
[2]"Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors."
Ngai N., Tsai F.Y., Coruzzi G.M.
Plant J. 12:1021-1034(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
Strain: cv. Feltham First.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52180 mRNA. Translation: CAA36430.1.
Y13322 Genomic DNA. Translation: CAA73763.1.
PIRAJPMN2. S11443.

3D structure databases

ProteinModelPortalP19252.
SMRP19252. Positions 1-517.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.976.

Proteomic databases

ProMEXP19252.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNS2_PEA
AccessionPrimary (citable) accession number: P19252
Secondary accession number(s): O49926
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways