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P19252

- ASNS2_PEA

UniProt

P19252 - ASNS2_PEA

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Protein

Asparagine synthetase, root [glutamine-hydrolyzing]

Gene

AS2

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei98 – 981GlutamineBy similarity
Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase, root [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AS2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 583582Asparagine synthetase, root [glutamine-hydrolyzing]PRO_0000056927Add
BLAST

Proteomic databases

ProMEXiP19252.

Expressioni

Tissue specificityi

Roots.

Inductioni

By darkness.

Structurei

3D structure databases

ProteinModelPortaliP19252.
SMRiP19252. Positions 1-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini237 – 516280Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19252-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDPSRAKRVR VLELSRRLKH RGPEWSGLHQ HGDCYLAQQR
60 70 80 90 100
LAIVDPASGD QPLFNEDNPS IVTVNGEIYN HEDLRKQLSN HTFRTGSDCD
110 120 130 140 150
VIAHLYEEYG EDFVDMLDGI FSFVPLDTRD NSYIVARDAI GVTSLYIGWG
160 170 180 190 200
LDGSVWISSE MKGLNDDCEH FECFPPGHLY SSKDSGFRRW YNPSWYSEAI
210 220 230 240 250
PSAPYDPLAL RHAFEKAVVK RLMTDVPFGV LLSGGLDSSL VASITSRYLA
260 270 280 290 300
TTKAAEQWGS KLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFTFTVQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTSIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KEEFHEETCR KIKALHQYDC QRANKSTYAW GLEARVPFLD
410 420 430 440 450
KAFINVAMNI DPENKMIKRD EGRIEKYILR KAFDDEENPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKAHAA KHVTDKMMLN AGNIFPHNTP NTKEAYYYRM
510 520 530 540 550
IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD PSGRAALGVH
560 570 580
DSAYENHNKV NKTVEFEKII PLEAAPVELA IQG
Length:583
Mass (Da):65,650
Last modified:January 23, 2007 - v3
Checksum:iE2C2DA6FEF1C72F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341E → D in CAA73763. (PubMed:9418044)Curated
Sequence conflicti45 – 451Y → F in CAA73763. (PubMed:9418044)Curated
Sequence conflicti48 – 481Q → H in CAA73763. (PubMed:9418044)Curated
Sequence conflicti78 – 781I → L in CAA73763. (PubMed:9418044)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52180 mRNA. Translation: CAA36430.1.
Y13322 Genomic DNA. Translation: CAA73763.1.
PIRiS11443. AJPMN2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52180 mRNA. Translation: CAA36430.1 .
Y13322 Genomic DNA. Translation: CAA73763.1 .
PIRi S11443. AJPMN2.

3D structure databases

ProteinModelPortali P19252.
SMRi P19252. Positions 1-517.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

ProMEXi P19252.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum."
    Tsai F.Y., Coruzzi G.M.
    EMBO J. 9:323-332(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Sparkle.
    Tissue: Root.
  2. "Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors."
    Ngai N., Tsai F.Y., Coruzzi G.M.
    Plant J. 12:1021-1034(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
    Strain: cv. Feltham First.

Entry informationi

Entry nameiASNS2_PEA
AccessioniPrimary (citable) accession number: P19252
Secondary accession number(s): O49926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3