Reviewed, UniProtKB/Swiss-Prot P19252 (ASNS2_PEA)

Names and origin

Protein names

Recommended name:
    Asparagine synthetase, root [glutamine-hydrolyzing]
    EC=6.3.5.4
Alternative name(s):
    Glutamine-dependent asparagine synthetase

Gene names

Name:

AS2

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Tissue specificity	Roots.
Induction	By darkness.
Sequence similarities	Contains 1 asparagine synthetase domain. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 583	582	Asparagine synthetase, root [glutamine-hydrolyzing]		PRO_0000056927
Regions
	Domain	2 – 185	184	Glutamine amidotransferase type-2
	Domain	237 – 516	280	Asparagine synthetase
Sites
	Active site	2	1	For GATase activity By similarity
Experimental info
	Sequence conflict	34	1	E → D in CAA73763. Ref.2
	Sequence conflict	45	1	Y → F in CAA73763. Ref.2
	Sequence conflict	48	1	Q → H in CAA73763. Ref.2
	Sequence conflict	78	1	I → L in CAA73763. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P19252-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E2C2DA6FEF1C72F1	FASTA	583	65,650
	10 20 30 40 50 60 MCGILAVLGC SDPSRAKRVR VLELSRRLKH RGPEWSGLHQ HGDCYLAQQR LAIVDPASGD 70 80 90 100 110 120 QPLFNEDNPS IVTVNGEIYN HEDLRKQLSN HTFRTGSDCD VIAHLYEEYG EDFVDMLDGI 130 140 150 160 170 180 FSFVPLDTRD NSYIVARDAI GVTSLYIGWG LDGSVWISSE MKGLNDDCEH FECFPPGHLY 190 200 210 220 230 240 SSKDSGFRRW YNPSWYSEAI PSAPYDPLAL RHAFEKAVVK RLMTDVPFGV LLSGGLDSSL 250 260 270 280 290 300 VASITSRYLA TTKAAEQWGS KLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFTFTVQDGI 310 320 330 340 350 360 DAIEDVIYHV ETYDVTSIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG GYLYFHKAPN 370 380 390 400 410 420 KEEFHEETCR KIKALHQYDC QRANKSTYAW GLEARVPFLD KAFINVAMNI DPENKMIKRD 430 440 450 460 470 480 EGRIEKYILR KAFDDEENPY LPKHILYRQK EQFSDGVGYS WIDGLKAHAA KHVTDKMMLN 490 500 510 520 530 540 AGNIFPHNTP NTKEAYYYRM IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD 550 560 570 580 PSGRAALGVH DSAYENHNKV NKTVEFEKII PLEAAPVELA IQG

References

[1]	"Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum." Tsai F.Y., Coruzzi G.M. EMBO J. 9:323-332(1990) [PubMed: 1968003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Sparkle. Tissue: Root.
[2]	"Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors." Ngai N., Tsai F.Y., Coruzzi G.M. Plant J. 12:1021-1034(1997) [PubMed: 9418044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84. Strain: cv. Feltham First.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52180 mRNA. Translation: CAA36430.1. Y13322 Genomic DNA. Translation: CAA73763.1.
PIR	AJPMN2. S11443.
3D structure databases
SMR	P19252. Positions 1-517.
ModBase	Search...
Protein family/group databases
MEROPS	C44.974.
Enzyme and pathway databases
BRENDA	6.3.5.4. 287.
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01536. asn_synth_AEB. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNS2_PEA

Accession

Primary (citable) accession number: P19252
Secondary accession number(s): O49926

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)