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P19252

- ASNS2_PEA

UniProt

P19252 - ASNS2_PEA

Protein

Asparagine synthetase, root [glutamine-hydrolyzing]

Gene

AS2

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei98 – 981GlutamineBy similarity
    Binding sitei231 – 2311ATP; via carbonyl oxygenBy similarity
    Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi341 – 3422ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Protein family/group databases

    MEROPSiC44.976.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase, root [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:AS2
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 583582Asparagine synthetase, root [glutamine-hydrolyzing]PRO_0000056927Add
    BLAST

    Proteomic databases

    ProMEXiP19252.

    Expressioni

    Tissue specificityi

    Roots.

    Inductioni

    By darkness.

    Structurei

    3D structure databases

    ProteinModelPortaliP19252.
    SMRiP19252. Positions 1-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 185184Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini237 – 516280Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni50 – 545Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19252-1 [UniParc]FASTAAdd to Basket

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    MCGILAVLGC SDPSRAKRVR VLELSRRLKH RGPEWSGLHQ HGDCYLAQQR    50
    LAIVDPASGD QPLFNEDNPS IVTVNGEIYN HEDLRKQLSN HTFRTGSDCD 100
    VIAHLYEEYG EDFVDMLDGI FSFVPLDTRD NSYIVARDAI GVTSLYIGWG 150
    LDGSVWISSE MKGLNDDCEH FECFPPGHLY SSKDSGFRRW YNPSWYSEAI 200
    PSAPYDPLAL RHAFEKAVVK RLMTDVPFGV LLSGGLDSSL VASITSRYLA 250
    TTKAAEQWGS KLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFTFTVQDGI 300
    DAIEDVIYHV ETYDVTSIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG 350
    GYLYFHKAPN KEEFHEETCR KIKALHQYDC QRANKSTYAW GLEARVPFLD 400
    KAFINVAMNI DPENKMIKRD EGRIEKYILR KAFDDEENPY LPKHILYRQK 450
    EQFSDGVGYS WIDGLKAHAA KHVTDKMMLN AGNIFPHNTP NTKEAYYYRM 500
    IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD PSGRAALGVH 550
    DSAYENHNKV NKTVEFEKII PLEAAPVELA IQG 583
    Length:583
    Mass (Da):65,650
    Last modified:January 23, 2007 - v3
    Checksum:iE2C2DA6FEF1C72F1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341E → D in CAA73763. (PubMed:9418044)Curated
    Sequence conflicti45 – 451Y → F in CAA73763. (PubMed:9418044)Curated
    Sequence conflicti48 – 481Q → H in CAA73763. (PubMed:9418044)Curated
    Sequence conflicti78 – 781I → L in CAA73763. (PubMed:9418044)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52180 mRNA. Translation: CAA36430.1.
    Y13322 Genomic DNA. Translation: CAA73763.1.
    PIRiS11443. AJPMN2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X52180 mRNA. Translation: CAA36430.1 .
    Y13322 Genomic DNA. Translation: CAA73763.1 .
    PIRi S11443. AJPMN2.

    3D structure databases

    ProteinModelPortali P19252.
    SMRi P19252. Positions 1-517.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.976.

    Proteomic databases

    ProMEXi P19252.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum."
      Tsai F.Y., Coruzzi G.M.
      EMBO J. 9:323-332(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Sparkle.
      Tissue: Root.
    2. "Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors."
      Ngai N., Tsai F.Y., Coruzzi G.M.
      Plant J. 12:1021-1034(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
      Strain: cv. Feltham First.

    Entry informationi

    Entry nameiASNS2_PEA
    AccessioniPrimary (citable) accession number: P19252
    Secondary accession number(s): O49926
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 89 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3