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Protein

Asparagine synthetase, root [glutamine-hydrolyzing]

Gene

AS2

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase, nodule [glutamine-hydrolyzing] (AS1), Asparagine synthetase, root [glutamine-hydrolyzing] (AS2)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei98GlutamineBy similarity1
Binding sitei231ATP; via carbonyl oxygenBy similarity1
Binding sitei267ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei343Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi341 – 342ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase, root [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AS2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569272 – 583Asparagine synthetase, root [glutamine-hydrolyzing]Add BLAST582

Proteomic databases

PRIDEiP19252.

Expressioni

Tissue specificityi

Roots.

Inductioni

By darkness.

Structurei

3D structure databases

ProteinModelPortaliP19252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 185Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST184
Domaini237 – 516Asparagine synthetaseAdd BLAST280

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 54Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19252-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGILAVLGC SDPSRAKRVR VLELSRRLKH RGPEWSGLHQ HGDCYLAQQR
60 70 80 90 100
LAIVDPASGD QPLFNEDNPS IVTVNGEIYN HEDLRKQLSN HTFRTGSDCD
110 120 130 140 150
VIAHLYEEYG EDFVDMLDGI FSFVPLDTRD NSYIVARDAI GVTSLYIGWG
160 170 180 190 200
LDGSVWISSE MKGLNDDCEH FECFPPGHLY SSKDSGFRRW YNPSWYSEAI
210 220 230 240 250
PSAPYDPLAL RHAFEKAVVK RLMTDVPFGV LLSGGLDSSL VASITSRYLA
260 270 280 290 300
TTKAAEQWGS KLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFTFTVQDGI
310 320 330 340 350
DAIEDVIYHV ETYDVTSIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG
360 370 380 390 400
GYLYFHKAPN KEEFHEETCR KIKALHQYDC QRANKSTYAW GLEARVPFLD
410 420 430 440 450
KAFINVAMNI DPENKMIKRD EGRIEKYILR KAFDDEENPY LPKHILYRQK
460 470 480 490 500
EQFSDGVGYS WIDGLKAHAA KHVTDKMMLN AGNIFPHNTP NTKEAYYYRM
510 520 530 540 550
IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD PSGRAALGVH
560 570 580
DSAYENHNKV NKTVEFEKII PLEAAPVELA IQG
Length:583
Mass (Da):65,650
Last modified:January 23, 2007 - v3
Checksum:iE2C2DA6FEF1C72F1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34E → D in CAA73763 (PubMed:9418044).Curated1
Sequence conflicti45Y → F in CAA73763 (PubMed:9418044).Curated1
Sequence conflicti48Q → H in CAA73763 (PubMed:9418044).Curated1
Sequence conflicti78I → L in CAA73763 (PubMed:9418044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52180 mRNA. Translation: CAA36430.1.
Y13322 Genomic DNA. Translation: CAA73763.1.
PIRiS11443. AJPMN2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52180 mRNA. Translation: CAA36430.1.
Y13322 Genomic DNA. Translation: CAA73763.1.
PIRiS11443. AJPMN2.

3D structure databases

ProteinModelPortaliP19252.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.976.

Proteomic databases

PRIDEiP19252.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS2_PEA
AccessioniPrimary (citable) accession number: P19252
Secondary accession number(s): O49926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.