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P19252

- ASNS2_PEA

UniProt

P19252 - ASNS2_PEA

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Protein

Asparagine synthetase, root [glutamine-hydrolyzing]

Gene
AS2
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei98 – 981Glutamine By similarity
Binding sitei231 – 2311ATP; via carbonyl oxygen By similarity
Binding sitei267 – 2671ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei343 – 3431Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi341 – 3422ATP By similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase, root [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AS2
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 583582Asparagine synthetase, root [glutamine-hydrolyzing]PRO_0000056927Add
BLAST

Proteomic databases

ProMEXiP19252.

Expressioni

Tissue specificityi

Roots.

Inductioni

By darkness.

Structurei

3D structure databases

ProteinModelPortaliP19252.
SMRiP19252. Positions 1-517.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2Add
BLAST
Domaini237 – 516280Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19252-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILAVLGC SDPSRAKRVR VLELSRRLKH RGPEWSGLHQ HGDCYLAQQR    50
LAIVDPASGD QPLFNEDNPS IVTVNGEIYN HEDLRKQLSN HTFRTGSDCD 100
VIAHLYEEYG EDFVDMLDGI FSFVPLDTRD NSYIVARDAI GVTSLYIGWG 150
LDGSVWISSE MKGLNDDCEH FECFPPGHLY SSKDSGFRRW YNPSWYSEAI 200
PSAPYDPLAL RHAFEKAVVK RLMTDVPFGV LLSGGLDSSL VASITSRYLA 250
TTKAAEQWGS KLHSFCVGLE GSPDLKAGKE VADYLGTVHH EFTFTVQDGI 300
DAIEDVIYHV ETYDVTSIRA STPMFLMSRK IKSLGVKWVI SGEGSDEIFG 350
GYLYFHKAPN KEEFHEETCR KIKALHQYDC QRANKSTYAW GLEARVPFLD 400
KAFINVAMNI DPENKMIKRD EGRIEKYILR KAFDDEENPY LPKHILYRQK 450
EQFSDGVGYS WIDGLKAHAA KHVTDKMMLN AGNIFPHNTP NTKEAYYYRM 500
IFERFFPQNS ARLTVPGGPT VACSTAKAVE WDAAWSNNLD PSGRAALGVH 550
DSAYENHNKV NKTVEFEKII PLEAAPVELA IQG 583
Length:583
Mass (Da):65,650
Last modified:January 23, 2007 - v3
Checksum:iE2C2DA6FEF1C72F1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341E → D in CAA73763. 1 Publication
Sequence conflicti45 – 451Y → F in CAA73763. 1 Publication
Sequence conflicti48 – 481Q → H in CAA73763. 1 Publication
Sequence conflicti78 – 781I → L in CAA73763. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52180 mRNA. Translation: CAA36430.1.
Y13322 Genomic DNA. Translation: CAA73763.1.
PIRiS11443. AJPMN2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52180 mRNA. Translation: CAA36430.1 .
Y13322 Genomic DNA. Translation: CAA73763.1 .
PIRi S11443. AJPMN2.

3D structure databases

ProteinModelPortali P19252.
SMRi P19252. Positions 1-517.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Proteomic databases

ProMEXi P19252.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum."
    Tsai F.Y., Coruzzi G.M.
    EMBO J. 9:323-332(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Sparkle.
    Tissue: Root.
  2. "Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors."
    Ngai N., Tsai F.Y., Coruzzi G.M.
    Plant J. 12:1021-1034(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-84.
    Strain: cv. Feltham First.

Entry informationi

Entry nameiASNS2_PEA
AccessioniPrimary (citable) accession number: P19252
Secondary accession number(s): O49926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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