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P19251

- ASNS1_PEA

UniProt

P19251 - ASNS1_PEA

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Protein

Asparagine synthetase, nodule [glutamine-hydrolyzing]

Gene
AS1
Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei98 – 981Glutamine By similarity
Binding sitei232 – 2321ATP; via carbonyl oxygen By similarity
Binding sitei268 – 2681ATP; via amide nitrogen and carbonyl oxygen By similarity
Sitei344 – 3441Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi342 – 3432ATP By similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase, nodule [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:AS1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 586585Asparagine synthetase, nodule [glutamine-hydrolyzing]PRO_0000056926Add
BLAST

Expressioni

Tissue specificityi

Root nodules.

Inductioni

By darkness.

Structurei

3D structure databases

ProteinModelPortaliP19251.
SMRiP19251. Positions 1-518.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 185184Glutamine amidotransferase type-2Add
BLAST
Domaini193 – 517325Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 545Glutamine binding By similarity
Regioni75 – 773Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19251-1 [UniParc]FASTAAdd to Basket

« Hide

MCGILAVLGC SDDSQAKRVR ILELSRRLKH RGPDWSGLHQ HGDNYLAHQR    50
LAIVDPASGD QPLFNEDKSI IVTVNGEIYN HEELRKQLPN HKFFTQCDCD 100
VIAHLYEEHG ENFVDMLDGI FSFVLLDTRD NSFIVARDAI GVTSLYIGWG 150
LDGSVWIASE LKGLNDECEH FEVFPPGHLY SSKEREFRRW YNPPWFNEAI 200
IPSTPYDPLV LRNAFEKAVI KRLMTDVPFG VLLSGGLDSS LVASVTARYL 250
AGTKAAKQWG AKLPSFCVGL KGAPDLKAGK EVADFLGTVH HEFEFTIQDG 300
IDAIEDVIYH TETYDVTTIR AATPMFLMSR KIKSSGVKWV ISGEGSDEIF 350
GGYLYFHKAP NREEFHQETC RKIKALHRYD CLRANKSTYA WGLEARVPFL 400
DKDFIKVAMD IDPEFKMIKH DEGRIEKWIL RKAFDDEENP YLPKHILYRQ 450
KEQFSDGVGY GWIDGIKDHA AKHVTDRMMF NASHIFPFNT PNTKEAYYYR 500
MIFERFFPQN SARLTVPGGP SVACSTEKAI EWDASWSNNL DPSGRAALGV 550
HVSAYEHQIN PVTKGVEPEK IIPKIGVSPL GVAIQT 586
Length:586
Mass (Da):66,353
Last modified:January 23, 2007 - v3
Checksum:i17C5019A3C8ED491
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52179 mRNA. Translation: CAA36429.1.
Y13321 Genomic DNA. Translation: CAA73762.1.
PIRiS11444. AJPMN1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X52179 mRNA. Translation: CAA36429.1 .
Y13321 Genomic DNA. Translation: CAA73762.1 .
PIRi S11444. AJPMN1.

3D structure databases

ProteinModelPortali P19251.
SMRi P19251. Positions 1-518.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.976.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum."
    Tsai F.Y., Coruzzi G.M.
    EMBO J. 9:323-332(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Sparkle.
    Tissue: Root nodule.
  2. "Light-induced transcriptional repression of the pea AS1 gene: identification of cis-elements and transfactors."
    Ngai N., Tsai F.Y., Coruzzi G.M.
    Plant J. 12:1021-1034(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 1-84.
    Strain: cv. Feltham First.

Entry informationi

Entry nameiASNS1_PEA
AccessioniPrimary (citable) accession number: P19251
Secondary accession number(s): O49925
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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