P19237 (TNNI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Troponin I, slow skeletal muscle

Alternative name(s):
Troponin I, slow-twitch isoform

Gene names

Name:

TNNI1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Subunit structure	Binds to actin and tropomyosin.
Tissue specificity	Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes. Ref.1 Ref.2
Sequence similarities	Belongs to the troponin I family.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	Actin-binding Calcium Metal-binding
Molecular function	Muscle protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	muscle filament sliding Traceable author statement. Source: Reactome regulation of striated muscle contraction Non-traceable author statement Ref.1. Source: UniProtKB ventricular cardiac muscle tissue morphogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Traceable author statement. Source: Reactome troponin complex Non-traceable author statement. Source: UniProtKB
Molecular_function	actin binding Non-traceable author statement. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tropomyosin binding Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Troponin I, slow skeletal muscle		PRO_0000186139
Regions
	Calcium binding	106 – 117	12	By similarity
	Region	1 – 48	48	Involved in binding TNC
	Region	97 – 118	22	Involved in binding TNC and actin
Natural variations
	Natural variant	67	1	R → W. Corresponds to variant rs2296695 [ dbSNP | Ensembl ].		VAR_052403
Experimental info
	Sequence conflict	4 – 5	2	VE → FQ in AC096677. Ref.8
	Sequence conflict	30	1	W → R in CAG29297. Ref.4
	Sequence conflict	103	1	D → A in CAD91135. Ref.5
	Sequence conflict	131	1	K → R in CAC44240. Ref.3
	Sequence conflict	143	1	K → T in CAG46793. Ref.4
	Sequence conflict	182 – 183	2	KS → NA in AAA61228. Ref.1
	Sequence conflict	182 – 183	2	KS → NA in CAG46827. Ref.4
	Sequence conflict	182 – 183	2	KS → NA in CAG46793. Ref.4
	Sequence conflict	182 – 183	2	KS → NA in CAG29297. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P19237 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7A8363CC7559B962	FASTA	187	21,692
	10 20 30 40 50 60 MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS 70 80 90 100 110 120 ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA 130 140 150 160 170 180 DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence, tissue-specific expression, and chromosomal mapping of the human slow-twitch skeletal muscle isoform of troponin I." Wade R., Eddy R., Shows T.B., Kedes L. Genomics 7:346-357(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Skeletal muscle.
[2]	"Structure and expression of the human slow twitch skeletal muscle troponin I gene." Corin S.J., Juhasz O., Zhu L., Conley P., Kedes L., Wade R. J. Biol. Chem. 269:10651-10659(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Tissue: Blood.
[3]	"Full length sequencing of some human and murine muscular transcripts (Telethon Italy project B41)." Frigimelica E., Ievolella C., Lanfranchi G. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[6]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[7]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04760 mRNA. Translation: AAA61228.1. L21910 , L21906, L21908, L21909 Genomic DNA. Translation: AAC14461.1. AJ315823 mRNA. Translation: CAC44240.1. CR450301 mRNA. Translation: CAG29297.1. CR541996 mRNA. Translation: CAG46793.1. CR542030 mRNA. Translation: CAG46827.1. AL831820 mRNA. Translation: CAD38534.1. AL831975 mRNA. Translation: CAH56221.1. BX510903 mRNA. Translation: CAD91135.1. AK223588 mRNA. Translation: BAD97308.1. AK311896 mRNA. Translation: BAG34837.1. AC096677 Genomic DNA. No translation available. BC012600 mRNA. Translation: AAH12600.1. BC012601 mRNA. Translation: AAH12601.1.
IPI	IPI00453474.
PIR	TPHUIW. A53740.
RefSeq	NP_003272.3. NM_003281.3.
UniGene	Hs.320890.
3D structure databases
ProteinModelPortal	P19237.
ModBase	Search...
Protein-protein interaction databases
IntAct	P19237. 2 interactions.
MINT	MINT-1467470.
PTM databases
PhosphoSite	P19237.
Polymorphism databases
DMDM	1351298.
Proteomic databases
PaxDb	P19237.
PRIDE	P19237.
Protocols and materials databases
DNASU	7135.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336092; ENSP00000337022; ENSG00000159173. ENST00000361379; ENSP00000354488; ENSG00000159173. ENST00000367312; ENSP00000356281; ENSG00000159173.
GeneID	7135.
KEGG	hsa:7135.
UCSC	uc001gwp.3. human.
Organism-specific databases
CTD	7135.
GeneCards	GC01M201372.
HGNC	HGNC:11945. TNNI1.
HPA	CAB009349. HPA028190.
MIM	191042. gene.
neXtProt	NX_P19237.
PharmGKB	PA36634.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG328514.
HOVERGEN	HBG052737.
InParanoid	P19237.
KO	K10371.
OMA	YLEEKSP.
OrthoDB	EOG4001KH.
Enzyme and pathway databases
Reactome	REACT_17044. Muscle contraction.
Gene expression databases
ArrayExpress	P19237.
Bgee	P19237.
Genevestigator	P19237.
GermOnline	ENSG00000159173. Homo sapiens.
Family and domain databases
InterPro	IPR001978. Troponin. [Graphical view]
Pfam	PF00992. Troponin. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TNNI1. human.
GenomeRNAi	7135.
NextBio	27917.
SOURCE	Search...

Entry information

Entry name

TNNI1_HUMAN

Accession

Primary (citable) accession number: P19237
Secondary accession number(s): A6NEH3 , A8MSJ0, Q659A5, Q6FGS7, Q6FGW1, Q6ICU2, Q86T57, Q96DT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.