NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial precursor

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P19234 (NDUV2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
NADH-ubiquinone oxidoreductase 24 kDa subunit

Gene names

Name:

Ndufv2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle tissue development Inferred from electronic annotation. Source: Compara mitochondrial electron transport, NADH to ubiquinone Inferred from Biological aspect of Ancestor. Source: RefGenome nervous system development Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial respiratory chain complex I Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 31

Mitochondrion

Chain

32 – 248

217

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

PRO_0000020005

Sites

Metal binding

134

Iron-sulfur (2Fe-2S) Potential

Metal binding

139

Iron-sulfur (2Fe-2S) Potential

Metal binding

175

Iron-sulfur (2Fe-2S) Potential

Metal binding

179

Iron-sulfur (2Fe-2S) Potential

Experimental info

Sequence conflict

245

Q → P in AAA41669. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P19234 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: 3A6AA85BB7A870B8
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FASTA

248

27,378

        10         20         30         40         50         60 
MFSLALRARA SGLTAQWGRH ARNLHKTAVQ NGAGGALFVH RDTPENNPDT PFDFTPENYE 

        70         80         90        100        110        120 
RIEAIVRNYP EGHRAAAVLP VLDLAQRQNG WLPISAMNKV AEVLQVPPMR VYEVATFYTM 

       130        140        150        160        170        180 
YNRKPVGKYH IQVCTTTPCM LRDSDSILET LQRKLGIKVG ETTPDKLFTL IEVECLGACV 

       190        200        210        220        230        240 
NAPMVQINDD YYEDLTPKDI EEIIDELRAG KVPKPGPRSG RFCCEPAGGL TSLTEPPKGP 


GFGVQAGL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[2]	"The amino acid sequence of the 24-kDa subunit, an iron-sulfur protein, of rat liver mitochondrial NADH dehydrogenase deduced from cDNA sequence." Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T. Biochem. Biophys. Res. Commun. 157:914-920(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-248.
[3]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 42-61; 75-87; 199-208 AND 222-248, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC058495 mRNA. Translation: AAH58495.1. M22756 mRNA. Translation: AAA41669.1.
IPI	IPI00367152.
PIR	A31868.
RefSeq	NP_112326.1. NM_031064.2.
UniGene	Rn.28882.
3D structure databases
ProteinModelPortal	P19234.
ModBase	Search...
Protein-protein interaction databases
IntAct	P19234. 1 interaction.
STRING	10116.ENSRNOP00000016965.
2D gel databases
UCD-2DPAGE	P19234.
Proteomic databases
PaxDb	P19234.
PRIDE	P19234.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016965; ENSRNOP00000016965; ENSRNOG00000042503.
GeneID	81728.
KEGG	rno:81728.
Organism-specific databases
CTD	4729.
RGD	621733. Ndufv2.
Phylogenomic databases
eggNOG	COG1905.
GeneTree	ENSGT00390000017580.
HOGENOM	HOG000257748.
HOVERGEN	HBG029601.
InParanoid	P19234.
KO	K03943.
OMA	SMAYIRV.
OrthoDB	EOG4QFWDW.
Gene expression databases
Genevestigator	P19234.
GermOnline	ENSRNOG00000032758. Rattus norvegicus.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR002023. 2Fe-2S_Ferredox. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10371. PTHR10371. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
PIRSF	PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. nuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615388.
PMAP-CutDB	P19234.

Entry information

Entry name

NDUV2_RAT

Accession

Primary (citable) accession number: P19234
Secondary accession number(s): Q6PDU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents