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P19234

- NDUV2_RAT

UniProt

P19234 - NDUV2_RAT

Protein

NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Gene

Ndufv2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi139 – 1391Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi175 – 1751Iron-sulfur (2Fe-2S)Sequence Analysis
    Metal bindingi179 – 1791Iron-sulfur (2Fe-2S)Sequence Analysis

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: RefGenome
    3. metal ion binding Source: UniProtKB-KW
    4. NADH dehydrogenase (ubiquinone) activity Source: RefGenome

    GO - Biological processi

    1. cardiac muscle tissue development Source: Ensembl
    2. mitochondrial electron transport, NADH to ubiquinone Source: RefGenome
    3. nervous system development Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    NADH-ubiquinone oxidoreductase 24 kDa subunit
    Gene namesi
    Name:Ndufv2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi621733. Ndufv2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial respiratory chain complex I Source: RefGenome

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3131MitochondrionAdd
    BLAST
    Chaini32 – 248217NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrialPRO_0000020005Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921Phosphotyrosine; by SRCBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP19234.
    PRIDEiP19234.

    2D gel databases

    UCD-2DPAGEP19234.

    Miscellaneous databases

    PMAP-CutDBP19234.

    Expressioni

    Gene expression databases

    GenevestigatoriP19234.

    Interactioni

    Subunit structurei

    Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme.

    Protein-protein interaction databases

    IntActiP19234. 1 interaction.
    STRINGi10116.ENSRNOP00000016965.

    Structurei

    3D structure databases

    ProteinModelPortaliP19234.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 24 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1905.
    GeneTreeiENSGT00390000017580.
    HOGENOMiHOG000257748.
    HOVERGENiHBG029601.
    InParanoidiP19234.
    KOiK03943.
    OMAiVHRDTPY.
    OrthoDBiEOG73JKW9.
    PhylomeDBiP19234.
    TreeFamiTF300004.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PANTHERiPTHR10371. PTHR10371. 1 hit.
    PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
    PROSITEiPS01099. COMPLEX1_24K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19234-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSLALRARA SGLTAQWGRH ARNLHKTAVQ NGAGGALFVH RDTPENNPDT    50
    PFDFTPENYE RIEAIVRNYP EGHRAAAVLP VLDLAQRQNG WLPISAMNKV 100
    AEVLQVPPMR VYEVATFYTM YNRKPVGKYH IQVCTTTPCM LRDSDSILET 150
    LQRKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDD YYEDLTPKDI 200
    EEIIDELRAG KVPKPGPRSG RFCCEPAGGL TSLTEPPKGP GFGVQAGL 248
    Length:248
    Mass (Da):27,378
    Last modified:December 6, 2005 - v2
    Checksum:i3A6AA85BB7A870B8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti245 – 2451Q → P in AAA41669. (PubMed:2974699)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC058495 mRNA. Translation: AAH58495.1.
    M22756 mRNA. Translation: AAA41669.1.
    PIRiA31868.
    RefSeqiNP_112326.1. NM_031064.2.
    UniGeneiRn.28882.

    Genome annotation databases

    EnsembliENSRNOT00000016965; ENSRNOP00000016965; ENSRNOG00000042503.
    GeneIDi81728.
    KEGGirno:81728.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC058495 mRNA. Translation: AAH58495.1 .
    M22756 mRNA. Translation: AAA41669.1 .
    PIRi A31868.
    RefSeqi NP_112326.1. NM_031064.2.
    UniGenei Rn.28882.

    3D structure databases

    ProteinModelPortali P19234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P19234. 1 interaction.
    STRINGi 10116.ENSRNOP00000016965.

    2D gel databases

    UCD-2DPAGE P19234.

    Proteomic databases

    PaxDbi P19234.
    PRIDEi P19234.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016965 ; ENSRNOP00000016965 ; ENSRNOG00000042503 .
    GeneIDi 81728.
    KEGGi rno:81728.

    Organism-specific databases

    CTDi 4729.
    RGDi 621733. Ndufv2.

    Phylogenomic databases

    eggNOGi COG1905.
    GeneTreei ENSGT00390000017580.
    HOGENOMi HOG000257748.
    HOVERGENi HBG029601.
    InParanoidi P19234.
    KOi K03943.
    OMAi VHRDTPY.
    OrthoDBi EOG73JKW9.
    PhylomeDBi P19234.
    TreeFami TF300004.

    Miscellaneous databases

    NextBioi 615388.
    PMAP-CutDB P19234.
    PROi P19234.

    Gene expression databases

    Genevestigatori P19234.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR002023. NuoE-like.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    PANTHERi PTHR10371. PTHR10371. 1 hit.
    PIRSFi PIRSF000216. NADH_DH_24kDa. 1 hit.
    SUPFAMi SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01958. nuoE_fam. 1 hit.
    PROSITEi PS01099. COMPLEX1_24K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    2. "The amino acid sequence of the 24-kDa subunit, an iron-sulfur protein, of rat liver mitochondrial NADH dehydrogenase deduced from cDNA sequence."
      Nishikimi M., Hosokawa Y., Toda H., Suzuki H., Ozawa T.
      Biochem. Biophys. Res. Commun. 157:914-920(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-248.
    3. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 42-61; 75-87; 199-208 AND 222-248, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiNDUV2_RAT
    AccessioniPrimary (citable) accession number: P19234
    Secondary accession number(s): Q6PDU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3