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Protein

Cytochrome P450 2C70

Gene

Cyp2c70

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi434 – 4341Iron (heme axial ligand)

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. monooxygenase activity Source: UniProtKB
  6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  7. oxygen binding Source: GO_Central
  8. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. epoxygenase P450 pathway Source: GO_Central
  2. exogenous drug catabolic process Source: GO_Central
  3. oxidation-reduction process Source: GO_Central
  4. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2C70 (EC:1.14.14.1)
Alternative name(s):
CYPIIC70
Cytochrome P-450Md
Cytochrome P450 P49
Gene namesi
Name:Cyp2c70
Synonyms:Cyp2c-70, Cyp2c22, P450md
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620368. Cyp2c22.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Cytochrome P450 2C70PRO_0000051727Add
BLAST

Proteomic databases

PaxDbiP19225.
PRIDEiP19225.

Expressioni

Gene expression databases

ExpressionAtlasiP19225. baseline.
GenevestigatoriP19225.

Interactioni

Protein-protein interaction databases

MINTiMINT-4563718.

Structurei

3D structure databases

ProteinModelPortaliP19225.
SMRiP19225. Positions 30-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP19225.
KOiK07413.
OrthoDBiEOG7RBZ85.
PhylomeDBiP19225.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19225-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFIFLGIW LSCLVFLFLW NQHHVRRKLP PGPTPLPIFG NILQVGVKNI
60 70 80 90 100
SKSMCMLAKE YGPVFTMYLG MKPTVVLYGY EVLKEALIDR GEEFSDKMHS
110 120 130 140 150
SMLSKVSQGL GIVFSNGEIW KQTRRFSLMV LRSMGMGKRT IENRIQEEVV
160 170 180 190 200
YLLEALRKTN GSPCDPSFLL ACVPCNVISS VIFQHRFDYS DEKFQKFIEN
210 220 230 240 250
FHTKIEILAS PWAQLCSAYP VLYYLPGIHN KFLKDVTEQK KFILMEINRH
260 270 280 290 300
RASLNLSNPQ DFIDYFLIKM EKEKHNEKSE FTMDNLIVTI GDLFGAGTET
310 320 330 340 350
TSSTIKYGLL LLLKYPEVTA KIQEEITRVI GRHRRPCMQD RNHMPYTDAV
360 370 380 390 400
LHEIQRYIDF VPIPLPRKTT QDVEFRGYHI PKGTSVMACL TSALHDDKEF
410 420 430 440 450
PNPEKFDPGH FLDEKGNFKK SDYFMAFSAG RRACIGEGLA RMEMFLILTS
460 470 480
ILQHFTLKPL VNPEDIDTTP VQPGLLSVPP PFELCFIPV
Length:489
Mass (Da):56,157
Last modified:October 31, 1990 - v1
Checksum:iE4C5B082AB81CB4D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501I → M in AAA40950 (PubMed:2263625).Curated
Sequence conflicti177 – 1771V → L in AAA40950 (PubMed:2263625).Curated
Sequence conflicti456 – 4561T → I in AAA40950 (PubMed:2263625).Curated
Sequence conflicti478 – 4781V → L in AAA40950 (PubMed:2263625).Curated
Sequence conflicti483 – 4831E → Q in AAA40950 (PubMed:2263625).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53477 mRNA. Translation: CAA37570.1.
M58041 mRNA. Translation: AAA40950.1.
PIRiS11160. A39257.
RefSeqiNP_612521.1. NM_138512.1.
UniGeneiRn.88025.

Genome annotation databases

EnsembliENSRNOT00000054724; ENSRNOP00000051607; ENSRNOG00000021924.
GeneIDi171518.
KEGGirno:171518.
UCSCiRGD:620368. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53477 mRNA. Translation: CAA37570.1.
M58041 mRNA. Translation: AAA40950.1.
PIRiS11160. A39257.
RefSeqiNP_612521.1. NM_138512.1.
UniGeneiRn.88025.

3D structure databases

ProteinModelPortaliP19225.
SMRiP19225. Positions 30-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4563718.

Proteomic databases

PaxDbiP19225.
PRIDEiP19225.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054724; ENSRNOP00000051607; ENSRNOG00000021924.
GeneIDi171518.
KEGGirno:171518.
UCSCiRGD:620368. rat.

Organism-specific databases

CTDi171518.
RGDi620368. Cyp2c22.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP19225.
KOiK07413.
OrthoDBiEOG7RBZ85.
PhylomeDBiP19225.
TreeFamiTF352043.

Miscellaneous databases

NextBioi622489.

Gene expression databases

ExpressionAtlasiP19225. baseline.
GenevestigatoriP19225.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "cDNA and deduced amino acid sequences of a male dominant P-450Md mRNA in rats."
    Nagata K., Sasamura H., Miyata M., Shimada M., Yamazoe Y., Kato R.
    Nucleic Acids Res. 18:4934-4934(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "A different cytochrome P450 form is induced in primary cultures of rat hepatocytes."
    Emi Y., Chijiiwa C., Omura T.
    Proc. Natl. Acad. Sci. U.S.A. 87:9746-9750(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiCP270_RAT
AccessioniPrimary (citable) accession number: P19225
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1990
Last sequence update: October 31, 1990
Last modified: February 3, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.