Reviewed, UniProtKB/Swiss-Prot P19225 (CP270_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2C70
    EC=1.14.14.1
Alternative name(s):
    CYPIIC70
    P-450Md
    P450 P49

Gene names

Name:	Cyp2c70
Synonyms:	Cyp2c-70, Cyp2c22, P450md

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Ref.2 Non-traceable author statement. Source: UniProtKB
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Cytochrome P450 2C70		PRO_0000051727
Sites
	Metal binding	434	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	50	1	I → M in AAA40950. Ref.2
	Sequence conflict	177	1	V → L in AAA40950. Ref.2
	Sequence conflict	456	1	T → I in AAA40950. Ref.2
	Sequence conflict	478	1	V → L in AAA40950. Ref.2
	Sequence conflict	483	1	E → Q in AAA40950. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P19225-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: E4C5B082AB81CB4D	FASTA	489	56,157
	10 20 30 40 50 60 MALFIFLGIW LSCLVFLFLW NQHHVRRKLP PGPTPLPIFG NILQVGVKNI SKSMCMLAKE 70 80 90 100 110 120 YGPVFTMYLG MKPTVVLYGY EVLKEALIDR GEEFSDKMHS SMLSKVSQGL GIVFSNGEIW 130 140 150 160 170 180 KQTRRFSLMV LRSMGMGKRT IENRIQEEVV YLLEALRKTN GSPCDPSFLL ACVPCNVISS 190 200 210 220 230 240 VIFQHRFDYS DEKFQKFIEN FHTKIEILAS PWAQLCSAYP VLYYLPGIHN KFLKDVTEQK 250 260 270 280 290 300 KFILMEINRH RASLNLSNPQ DFIDYFLIKM EKEKHNEKSE FTMDNLIVTI GDLFGAGTET 310 320 330 340 350 360 TSSTIKYGLL LLLKYPEVTA KIQEEITRVI GRHRRPCMQD RNHMPYTDAV LHEIQRYIDF 370 380 390 400 410 420 VPIPLPRKTT QDVEFRGYHI PKGTSVMACL TSALHDDKEF PNPEKFDPGH FLDEKGNFKK 430 440 450 460 470 480 SDYFMAFSAG RRACIGEGLA RMEMFLILTS ILQHFTLKPL VNPEDIDTTP VQPGLLSVPP PFELCFIPV

References

[1]	"cDNA and deduced amino acid sequences of a male dominant P-450Md mRNA in rats." Nagata K., Sasamura H., Miyata M., Shimada M., Yamazoe Y., Kato R. Nucleic Acids Res. 18:4934-4934(1990) [PubMed: 2395662] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"A different cytochrome P450 form is induced in primary cultures of rat hepatocytes." Emi Y., Chijiiwa C., Omura T. Proc. Natl. Acad. Sci. U.S.A. 87:9746-9750(1990) [PubMed: 2263625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53477 mRNA. Translation: CAA37570.1. M58041 mRNA. Translation: AAA40950.1.
IPI	IPI00778443.
PIR	A39257. S11160.
RefSeq	NP_612521.1.
UniGene	Rn.88025
3D structure databases
HSSP	HSSP built from PDB template 1OG2 based on UniProtKB P11712.
ModBase	Search...
Protein-protein interaction databases
STRING	P19225.
Proteomic databases
PRIDE	P19225.
Genome annotation databases
Ensembl	ENSRNOT00000054724; ENSRNOP00000051607; ENSRNOG00000021924; Rattus norvegicus. [Genome view] ENSRNOT00000054725; ENSRNOP00000051608; ENSRNOG00000021924; Rattus norvegicus. [Genome view]
GeneID	171518.
KEGG	rno:171518.
UCSC	NM_138512. rat.
Organism-specific databases
CTD	171518.
RGD	620368. Cyp2c22.
Phylogenomic databases
HOVERGEN	P19225.
Enzyme and pathway databases
BRENDA	1.14.14.1. 248.
Gene expression databases
ArrayExpress	P19225.
Genevestigator	P19225.
GermOnline	ENSRNOG00000021924. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622489.

Entry information

Entry name

CP270_RAT

Accession

Primary (citable) accession number: P19225

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	October 13, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)