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Protein

Methylated-DNA--protein-cysteine methyltransferase, inducible

Gene

adaB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.2 Publications

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation1 Publication
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei141Nucleophile; methyl group acceptor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciBSUB:BSU01820-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase, inducible (EC:2.1.1.631 Publication)
Alternative name(s):
O-6-methylguanine-DNA alkyltransferase
O6-methylguanine-DNA methyltransferase
Short name:
MGMT
Gene namesi
Name:adaB
Ordered Locus Names:BSU01820
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi141C → Y: Loss of activity. 1 Publication1
Mutagenesisi167L → P: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393741 – 179Methylated-DNA--protein-cysteine methyltransferase, inducibleAdd BLAST179

Proteomic databases

PaxDbiP19220.

Expressioni

Inductioni

Up-regulated by methylated AdaA in response to the exposure to alkylating agents such as MNNG.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001028.

Structurei

3D structure databases

ProteinModelPortaliP19220.
SMRiP19220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiENOG4105K85. Bacteria.
COG0350. LUCA.
HOGENOMiHOG000244137.
InParanoidiP19220.
KOiK13531.
OMAiRFHIAAT.
PhylomeDBiP19220.

Family and domain databases

CDDicd06445. ATase. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR001497. MethylDNA_cys_MeTrfase_AS.
IPR014048. MethylDNA_cys_MeTrfase_DNA-bd.
IPR036217. MethylDNA_cys_MeTrfase_DNAb.
IPR008332. MethylG_MeTrfase_N.
IPR036631. MGMT_N_sf.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF01035. DNA_binding_1. 1 hit.
PF02870. Methyltransf_1N. 1 hit.
SUPFAMiSSF46767. SSF46767. 1 hit.
SSF53155. SSF53155. 1 hit.
TIGRFAMsiTIGR00589. ogt. 1 hit.
PROSITEiView protein in PROSITE
PS00374. MGMT. 1 hit.

Sequencei

Sequence statusi: Complete.

P19220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METNKPTLYW SLLMFKDWNF YIASTLKGLV FVGSQNKPIE ELFEWARKRF
60 70 80 90 100
PGSLLVEDDD KLEPYAVEIT QYLEGKRKNF TVPVEYAGTQ FQLAVWNALC
110 120 130 140 150
EIPYGQTKSY SDIANDINKP AAVRAVGAAI GANPVLITVP CHRVIGKNGS
160 170
LTGYRGGFEM KTLLLDLEKR ASSEMDVPH
Length:179
Mass (Da):20,124
Last modified:November 1, 1990 - v1
Checksum:iBC30C65C6B76B64A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53399 Genomic DNA. Translation: CAA37476.1.
AB006424 Genomic DNA. Translation: BAA33075.1.
AL009126 Genomic DNA. Translation: CAB11958.1.
PIRiS11564. XUBSMB.
RefSeqiNP_388063.1. NC_000964.3.
WP_003246311.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB11958; CAB11958; BSU01820.
GeneIDi938673.
KEGGibsu:BSU01820.
PATRICifig|224308.179.peg.188.

Similar proteinsi

Entry informationi

Entry nameiADAB_BACSU
AccessioniPrimary (citable) accession number: P19220
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: February 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome