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Protein

Replication-associated protein ORF4

Gene

ORF4

Organism
Chlamydia phage 1 (Bacteriophage Chp1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein ORF4 is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion (By similarity).By similarity

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei251 – 2511O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
Active sitei255 – 2551O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA ligase (ATP) activity Source: UniProtKB-EC
  3. endonuclease activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Ligase, Nuclease

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein ORF4 (EC:3.1.21.-, EC:6.5.1.1)
Alternative name(s):
Rep
Gene namesi
ORF Names:ORF4
OrganismiChlamydia phage 1 (Bacteriophage Chp1)
Taxonomic identifieri10857 [NCBI]
Taxonomic lineageiVirusesssDNA virusesMicroviridaeGokushovirinaeChlamydiamicrovirus
Virus hostiChlamydophila psittaci (Chlamydia psittaci) [TaxID: 83554]
ProteomesiUP000002125 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Replication-associated protein ORF4PRO_0000066092Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the microviridae Rep protein family.Curated

Sequencei

Sequence statusi: Complete.

P19189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYSLDSYLI SVYIRLTQRK SDYMCTNPII PIVQYKVPVK SSLDVVDWSK
60 70 80 90 100
FRSNFKANLF FFEKNVVRRA VSNVDEAFRF TEQLKQVSYL STFDLDGYHQ
110 120 130 140 150
VKQFSFPLPC RKCSECLQKR SKDLAVQATM EARSHEENSV LILTYDNDHL
160 170 180 190 200
GDNILDYDHI RVFQKRLRRY VDYHYGKKIK FLTVGEYGDK KGRMHWHMIV
210 220 230 240 250
FGWKPKSEEQ LEPYLGGKYR TDVRYRSRKL KELWKFGYVD VDEATDGNIF
260 270 280 290 300
YVARYVQKKF VVGCDLDSSK SSSRREKKTA SQALGLDYFF SYLRQFLKTK
310 320 330 340 350
RIVLNGFRYG FPRYFKDLLR KLVSEDSEFD TEYYNALRKR LLSVCSYSMV
360 370 380 390
NKYFTYLECL VEVLPVLNFH DLYQRALRYM DQSILKPHAS DHDGEYNTT
Length:399
Mass (Da):47,560
Last modified:November 1, 1990 - v1
Checksum:iB27A7834D29D1990
GO

Sequence cautioni

The sequence BAA00512.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00624 Genomic DNA. Translation: BAA00511.1.
D00624 Genomic DNA. Translation: BAA00512.1. Different initiation.
RefSeqiNP_044320.1. NC_001741.1.
NP_044321.1. NC_001741.1.

Genome annotation databases

GeneIDi1261210.
1261211.
KEGGivg:1261210.
vg:1261211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00624 Genomic DNA. Translation: BAA00511.1.
D00624 Genomic DNA. Translation: BAA00512.1. Different initiation.
RefSeqiNP_044320.1. NC_001741.1.
NP_044321.1. NC_001741.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1261210.
1261211.
KEGGivg:1261210.
vg:1261211.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Analysis of the complete nucleotide sequence of Chp1, a phage which infects avian Chlamydia psittaci."
    Storey C.C., Lusher M., Richmond S.J.
    J. Gen. Virol. 70:3381-3390(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREP_BPCHP
AccessioniPrimary (citable) accession number: P19189
Secondary accession number(s): P19186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.