SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P19189

- REP_BPCHP

UniProt

P19189 - REP_BPCHP

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Replication-associated protein ORF4

Gene
ORF4
Organism
Chlamydia phage 1 (Bacteriophage Chp1)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein ORF4 is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion By similarity.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei251 – 2511O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Active sitei255 – 2551O-(5'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA ligase (ATP) activity Source: UniProtKB-EC
  3. endonuclease activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Ligase, Nuclease

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein ORF4 (EC:3.1.21.-, EC:6.5.1.1)
Alternative name(s):
Rep
Gene namesi
ORF Names:ORF4
OrganismiChlamydia phage 1 (Bacteriophage Chp1)
Taxonomic identifieri10857 [NCBI]
Taxonomic lineageiVirusesssDNA virusesMicroviridaeGokushovirinaeChlamydiamicrovirus
Virus hostiChlamydophila psittaci (Chlamydia psittaci) [TaxID: 83554]
ProteomesiUP000002125: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Replication-associated protein ORF4PRO_0000066092Add
BLAST

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Complete.

P19189-1 [UniParc]FASTAAdd to Basket

« Hide

MRYSLDSYLI SVYIRLTQRK SDYMCTNPII PIVQYKVPVK SSLDVVDWSK    50
FRSNFKANLF FFEKNVVRRA VSNVDEAFRF TEQLKQVSYL STFDLDGYHQ 100
VKQFSFPLPC RKCSECLQKR SKDLAVQATM EARSHEENSV LILTYDNDHL 150
GDNILDYDHI RVFQKRLRRY VDYHYGKKIK FLTVGEYGDK KGRMHWHMIV 200
FGWKPKSEEQ LEPYLGGKYR TDVRYRSRKL KELWKFGYVD VDEATDGNIF 250
YVARYVQKKF VVGCDLDSSK SSSRREKKTA SQALGLDYFF SYLRQFLKTK 300
RIVLNGFRYG FPRYFKDLLR KLVSEDSEFD TEYYNALRKR LLSVCSYSMV 350
NKYFTYLECL VEVLPVLNFH DLYQRALRYM DQSILKPHAS DHDGEYNTT 399
Length:399
Mass (Da):47,560
Last modified:November 1, 1990 - v1
Checksum:iB27A7834D29D1990
GO

Sequence cautioni

The sequence BAA00512.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00624 Genomic DNA. Translation: BAA00511.1.
D00624 Genomic DNA. Translation: BAA00512.1. Different initiation.
RefSeqiNP_044320.1. NC_001741.1.
NP_044321.1. NC_001741.1.

Genome annotation databases

GeneIDi1261210.
1261211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00624 Genomic DNA. Translation: BAA00511.1 .
D00624 Genomic DNA. Translation: BAA00512.1 . Different initiation.
RefSeqi NP_044320.1. NC_001741.1.
NP_044321.1. NC_001741.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1261210.
1261211.

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Analysis of the complete nucleotide sequence of Chp1, a phage which infects avian Chlamydia psittaci."
    Storey C.C., Lusher M., Richmond S.J.
    J. Gen. Virol. 70:3381-3390(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiREP_BPCHP
AccessioniPrimary (citable) accession number: P19189
Secondary accession number(s): P19186
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi