Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P19189

- REP_BPCHP

UniProt

P19189 - REP_BPCHP

Protein

Replication-associated protein ORF4

Gene

ORF4

Organism
Chlamydia phage 1 (Bacteriophage Chp1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein ORF4 is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion By similarity.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei251 – 2511O-(5'-phospho-DNA)-tyrosine intermediateBy similarity
    Active sitei255 – 2551O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA ligase (ATP) activity Source: UniProtKB-EC
    3. endonuclease activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Ligase, Nuclease

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replication-associated protein ORF4 (EC:3.1.21.-, EC:6.5.1.1)
    Alternative name(s):
    Rep
    Gene namesi
    ORF Names:ORF4
    OrganismiChlamydia phage 1 (Bacteriophage Chp1)
    Taxonomic identifieri10857 [NCBI]
    Taxonomic lineageiVirusesssDNA virusesMicroviridaeGokushovirinaeChlamydiamicrovirus
    Virus hostiChlamydophila psittaci (Chlamydia psittaci) [TaxID: 83554]
    ProteomesiUP000002125: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 399399Replication-associated protein ORF4PRO_0000066092Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the microviridae Rep protein family.Curated

    Sequencei

    Sequence statusi: Complete.

    P19189-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRYSLDSYLI SVYIRLTQRK SDYMCTNPII PIVQYKVPVK SSLDVVDWSK    50
    FRSNFKANLF FFEKNVVRRA VSNVDEAFRF TEQLKQVSYL STFDLDGYHQ 100
    VKQFSFPLPC RKCSECLQKR SKDLAVQATM EARSHEENSV LILTYDNDHL 150
    GDNILDYDHI RVFQKRLRRY VDYHYGKKIK FLTVGEYGDK KGRMHWHMIV 200
    FGWKPKSEEQ LEPYLGGKYR TDVRYRSRKL KELWKFGYVD VDEATDGNIF 250
    YVARYVQKKF VVGCDLDSSK SSSRREKKTA SQALGLDYFF SYLRQFLKTK 300
    RIVLNGFRYG FPRYFKDLLR KLVSEDSEFD TEYYNALRKR LLSVCSYSMV 350
    NKYFTYLECL VEVLPVLNFH DLYQRALRYM DQSILKPHAS DHDGEYNTT 399
    Length:399
    Mass (Da):47,560
    Last modified:November 1, 1990 - v1
    Checksum:iB27A7834D29D1990
    GO

    Sequence cautioni

    The sequence BAA00512.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00624 Genomic DNA. Translation: BAA00511.1.
    D00624 Genomic DNA. Translation: BAA00512.1. Different initiation.
    RefSeqiNP_044320.1. NC_001741.1.
    NP_044321.1. NC_001741.1.

    Genome annotation databases

    GeneIDi1261210.
    1261211.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00624 Genomic DNA. Translation: BAA00511.1 .
    D00624 Genomic DNA. Translation: BAA00512.1 . Different initiation.
    RefSeqi NP_044320.1. NC_001741.1.
    NP_044321.1. NC_001741.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1261210.
    1261211.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the complete nucleotide sequence of Chp1, a phage which infects avian Chlamydia psittaci."
      Storey C.C., Lusher M., Richmond S.J.
      J. Gen. Virol. 70:3381-3390(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiREP_BPCHP
    AccessioniPrimary (citable) accession number: P19189
    Secondary accession number(s): P19186
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3