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P19189 (REP_BPCHP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication-associated protein ORF4

EC=3.1.21.-
EC=6.5.1.1
Alternative name(s):
Rep
Gene names
ORF Names:ORF4
OrganismChlamydia phage 1 (Bacteriophage Chp1) [Reference proteome]
Taxonomic identifier10857 [NCBI]
Taxonomic lineageVirusesssDNA virusesMicroviridaeGokushovirinaeChlamydiamicrovirus
Virus hostChlamydophila psittaci (Chlamydia psittaci) [TaxID: 83554]

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an essential role in viral DNA replication. Binds the origin of replication and cleaves the dsDNA replicative form I (RFI) and becomes covalently bound to it via phosphotyrosine bond, generating the dsDNA replicative form II (RFII). In turn, viral DNA replication initiates at the 3'-OH of the cleavage site. After one round of rolling circle synthesis, protein ORF4 is linked to the newly synthesized ssDNA and joins the ends of the displaced strand to generate a circular single-stranded molecule ready to be packed into a virion By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Sequence similarities

Belongs to the microviridae Rep protein family.

Sequence caution

The sequence BAA00512.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 399399Replication-associated protein ORF4
PRO_0000066092

Sites

Active site2511O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Active site2551O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P19189 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: B27A7834D29D1990

FASTA39947,560
        10         20         30         40         50         60 
MRYSLDSYLI SVYIRLTQRK SDYMCTNPII PIVQYKVPVK SSLDVVDWSK FRSNFKANLF 

        70         80         90        100        110        120 
FFEKNVVRRA VSNVDEAFRF TEQLKQVSYL STFDLDGYHQ VKQFSFPLPC RKCSECLQKR 

       130        140        150        160        170        180 
SKDLAVQATM EARSHEENSV LILTYDNDHL GDNILDYDHI RVFQKRLRRY VDYHYGKKIK 

       190        200        210        220        230        240 
FLTVGEYGDK KGRMHWHMIV FGWKPKSEEQ LEPYLGGKYR TDVRYRSRKL KELWKFGYVD 

       250        260        270        280        290        300 
VDEATDGNIF YVARYVQKKF VVGCDLDSSK SSSRREKKTA SQALGLDYFF SYLRQFLKTK 

       310        320        330        340        350        360 
RIVLNGFRYG FPRYFKDLLR KLVSEDSEFD TEYYNALRKR LLSVCSYSMV NKYFTYLECL 

       370        380        390 
VEVLPVLNFH DLYQRALRYM DQSILKPHAS DHDGEYNTT 

« Hide

References

[1]"Analysis of the complete nucleotide sequence of Chp1, a phage which infects avian Chlamydia psittaci."
Storey C.C., Lusher M., Richmond S.J.
J. Gen. Virol. 70:3381-3390(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00624 Genomic DNA. Translation: BAA00511.1.
D00624 Genomic DNA. Translation: BAA00512.1. Different initiation.
RefSeqNP_044320.1. NC_001741.1.
NP_044321.1. NC_001741.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1261210.
1261211.

Phylogenomic databases

ProtClustDBPHA0330.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameREP_BPCHP
AccessionPrimary (citable) accession number: P19189
Secondary accession number(s): P19186
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families