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Protein

Acidic endochitinase

Gene

CHIB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This protein functions as a defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei156Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellular response to water deprivation Source: TAIR
  • chitin catabolic process Source: GO_Central
  • polysaccharide catabolic process Source: UniProtKB-KW
  • response to cold Source: TAIR
  • response to light intensity Source: TAIR
  • response to salt stress Source: TAIR
  • response to wounding Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT5G24090-MONOMER.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic endochitinase (EC:3.2.1.14)
Gene namesi
Name:CHIB1
Ordered Locus Names:At5g24090
ORF Names:MZF18.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G24090.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Add BLAST30
ChainiPRO_000001191331 – 302Acidic endochitinaseAdd BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 96By similarity
Disulfide bondi79 ↔ 86By similarity
Disulfide bondi188 ↔ 217By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19172.
PRIDEiP19172.

Expressioni

Inductioni

Expression of the acidic chitinase gene was not detected in normal, untreated plants nor in plants treated with ethylene or salicylic acid.

Gene expression databases

GenevisibleiP19172. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G24090.1.

Structurei

3D structure databases

ProteinModelPortaliP19172.
SMRiP19172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4701. Eukaryota.
ENOG4111GPA. LUCA.
InParanoidiP19172.
KOiK01183.
OMAiCTHFGSQ.
OrthoDBiEOG09360IVR.
PhylomeDBiP19172.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNMTLRKHV IYFLFFISCS LSKPSDASRG GIAIYWGQNG NEGNLSATCA
60 70 80 90 100
TGRYAYVNVA FLVKFGNGQT PELNLAGHCN PAANTCTHFG SQVKDCQSRG
110 120 130 140 150
IKVMLSLGGG IGNYSIGSRE DAKVIADYLW NNFLGGKSSS RPLGDAVLDG
160 170 180 190 200
IDFNIELGSP QHWDDLARTL SKFSHRGRKI YLTGAPQCPF PDRLMGSALN
210 220 230 240 250
TKRFDYVWIQ FYNNPPCSYS SGNTQNLFDS WNKWTTSIAA QKFFLGLPAA
260 270 280 290 300
PEAAGSGYIP PDVLTSQILP TLKKSRKYGG VMLWSKFWDD KNGYSSSILA

SV
Length:302
Mass (Da):33,097
Last modified:January 10, 2003 - v2
Checksum:i522AEB092E6EDCA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255G → D in AAA32768 (PubMed:16667600).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti22S → T in strain: cv. Ci-0. 1
Natural varianti42E → A in strain: cv. Es-0. 1
Natural varianti69Q → R in strain: cv. Ci-0. 1
Natural varianti120E → K in strain: cv. Ci-0. 1
Natural varianti125I → V in strain: cv. Ci-0. 1
Natural varianti169T → S in strain: cv. Ci-0, cv. Ita-0 and cv. Pog-0. 1
Natural varianti173F → Y in strain: cv. Ci-0. 1
Natural varianti174S → T in strain: cv. Yo-0. 1
Natural varianti180I → V in strain: cv. Ita-0 and cv. Pog-0. 1
Natural varianti217C → W in strain: cv. Mt-0. 1
Natural varianti220S → T in strain: cv. Ita-0 and cv. Pog-0. 1
Natural varianti239A → T in strain: cv. Chi-0. 1
Natural varianti243F → I in strain: cv. Ita-0. 1
Natural varianti243F → L in strain: cv. Pog-0. 1
Natural varianti257G → V in strain: cv. Yo-0. 1
Natural varianti274K → N in strain: cv. Ci-0. 1
Natural varianti299L → S in strain: cv. Bla-10. 1
Natural varianti299L → V in strain: cv. Bs-1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34107 Genomic DNA. Translation: AAA32768.1.
AB006055 Genomic DNA. Translation: BAA21860.1.
AB006056 Genomic DNA. Translation: BAA21861.1.
AB006057 Genomic DNA. Translation: BAA21862.1.
AB006058 Genomic DNA. Translation: BAA21863.1.
AB006059 Genomic DNA. Translation: BAA21864.1.
AB006060 Genomic DNA. Translation: BAA21865.1.
AB006061 Genomic DNA. Translation: BAA21866.1.
AB006062 Genomic DNA. Translation: BAA21867.1.
AB006063 Genomic DNA. Translation: BAA21868.1.
AB006064 Genomic DNA. Translation: BAA21869.1.
AB006065 Genomic DNA. Translation: BAA21870.1.
AB006066 Genomic DNA. Translation: BAA21871.1.
AB006067 Genomic DNA. Translation: BAA21872.1.
AB006068 Genomic DNA. Translation: BAA21873.1.
AB006069 Genomic DNA. Translation: BAA21874.1.
AB009056 Genomic DNA. Translation: BAB08732.1.
CP002688 Genomic DNA. Translation: AED93255.1.
BT020526 mRNA. Translation: AAW49295.1.
BT021931 mRNA. Translation: AAX49380.1.
PIRiA45511.
RefSeqiNP_197797.1. NM_122314.3.
UniGeneiAt.43218.

Genome annotation databases

EnsemblPlantsiAT5G24090.1; AT5G24090.1; AT5G24090.
GeneIDi832474.
GrameneiAT5G24090.1; AT5G24090.1; AT5G24090.
KEGGiath:AT5G24090.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34107 Genomic DNA. Translation: AAA32768.1.
AB006055 Genomic DNA. Translation: BAA21860.1.
AB006056 Genomic DNA. Translation: BAA21861.1.
AB006057 Genomic DNA. Translation: BAA21862.1.
AB006058 Genomic DNA. Translation: BAA21863.1.
AB006059 Genomic DNA. Translation: BAA21864.1.
AB006060 Genomic DNA. Translation: BAA21865.1.
AB006061 Genomic DNA. Translation: BAA21866.1.
AB006062 Genomic DNA. Translation: BAA21867.1.
AB006063 Genomic DNA. Translation: BAA21868.1.
AB006064 Genomic DNA. Translation: BAA21869.1.
AB006065 Genomic DNA. Translation: BAA21870.1.
AB006066 Genomic DNA. Translation: BAA21871.1.
AB006067 Genomic DNA. Translation: BAA21872.1.
AB006068 Genomic DNA. Translation: BAA21873.1.
AB006069 Genomic DNA. Translation: BAA21874.1.
AB009056 Genomic DNA. Translation: BAB08732.1.
CP002688 Genomic DNA. Translation: AED93255.1.
BT020526 mRNA. Translation: AAW49295.1.
BT021931 mRNA. Translation: AAX49380.1.
PIRiA45511.
RefSeqiNP_197797.1. NM_122314.3.
UniGeneiAt.43218.

3D structure databases

ProteinModelPortaliP19172.
SMRiP19172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G24090.1.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Proteomic databases

PaxDbiP19172.
PRIDEiP19172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G24090.1; AT5G24090.1; AT5G24090.
GeneIDi832474.
GrameneiAT5G24090.1; AT5G24090.1; AT5G24090.
KEGGiath:AT5G24090.

Organism-specific databases

TAIRiAT5G24090.

Phylogenomic databases

eggNOGiKOG4701. Eukaryota.
ENOG4111GPA. LUCA.
InParanoidiP19172.
KOiK01183.
OMAiCTHFGSQ.
OrthoDBiEOG09360IVR.
PhylomeDBiP19172.

Enzyme and pathway databases

BioCyciARA:AT5G24090-MONOMER.

Miscellaneous databases

PROiP19172.

Gene expression databases

GenevisibleiP19172. AT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001223. Glyco_hydro18_cat.
IPR001579. Glyco_hydro_18_chit_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS01095. CHITINASE_18. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHIA_ARATH
AccessioniPrimary (citable) accession number: P19172
Secondary accession number(s): O22065
, O22066, O22067, O22068, O22069, O22070, O22071, O22072, O22073, O24614, Q5HYZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 10, 2003
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.