Reviewed, UniProtKB/Swiss-Prot P19172 (CHIA_ARATH)

Names and origin

Protein names

Recommended name:
    Acidic endochitinase
    EC=3.2.1.14

Gene names

Name:	CHIB1
Ordered Locus Names:	At5g24090
ORF Names:	MZF18.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein functions as a defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space. Note: Intercellular space of infected plants.
Induction	Expression of the acidic chitinase gene was not detected in normal, untreated plants nor in plants treated with ethylene or salicylic acid.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class III subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30
	Chain	31 – 302	272	Acidic endochitinase		PRO_0000011913
Sites
	Active site	156	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	49 ↔ 96		By similarity
	Disulfide bond	79 ↔ 86		By similarity
	Disulfide bond	188 ↔ 217		By similarity
Natural variations
	Natural variant	22	1	S → T in strain: cv. Ci-0.
	Natural variant	42	1	E → A in strain: cv. Es-0.
	Natural variant	69	1	Q → R in strain: cv. Ci-0.
	Natural variant	120	1	E → K in strain: cv. Ci-0.
	Natural variant	125	1	I → V in strain: cv. Ci-0.
	Natural variant	169	1	T → S in strain: cv. Ci-0, cv. Ita-0 and cv. Pog-0.
	Natural variant	173	1	F → Y in strain: cv. Ci-0.
	Natural variant	174	1	S → T in strain: cv. Yo-0.
	Natural variant	180	1	I → V in strain: cv. Ita-0 and cv. Pog-0.
	Natural variant	217	1	C → W in strain: cv. Mt-0.
	Natural variant	220	1	S → T in strain: cv. Ita-0 and cv. Pog-0.
	Natural variant	239	1	A → T in strain: cv. Chi-0.
	Natural variant	243	1	F → I in strain: cv. Ita-0.
	Natural variant	243	1	F → L in strain: cv. Pog-0.
	Natural variant	257	1	G → V in strain: cv. Yo-0.
	Natural variant	274	1	K → N in strain: cv. Ci-0.
	Natural variant	299	1	L → S in strain: cv. Bla-10.
	Natural variant	299	1	L → V in strain: cv. Bs-1.
Experimental info
	Sequence conflict	255	1	G → D in AAA32768. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P19172-1 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 522AEB092E6EDCA8	FASTA	302	33,097
	10 20 30 40 50 60 MTNMTLRKHV IYFLFFISCS LSKPSDASRG GIAIYWGQNG NEGNLSATCA TGRYAYVNVA 70 80 90 100 110 120 FLVKFGNGQT PELNLAGHCN PAANTCTHFG SQVKDCQSRG IKVMLSLGGG IGNYSIGSRE 130 140 150 160 170 180 DAKVIADYLW NNFLGGKSSS RPLGDAVLDG IDFNIELGSP QHWDDLARTL SKFSHRGRKI 190 200 210 220 230 240 YLTGAPQCPF PDRLMGSALN TKRFDYVWIQ FYNNPPCSYS SGNTQNLFDS WNKWTTSIAA 250 260 270 280 290 300 QKFFLGLPAA PEAAGSGYIP PDVLTSQILP TLKKSRKYGG VMLWSKFWDD KNGYSSSILA SV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the genes encoding basic and acidic chitinase in Arabidopsis thaliana." Samac D.A., Hironaka C.M., Yallaly P.E., Shah D.M. Plant Physiol. 93:907-914(1990) [PubMed: 16667600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Nucleotide polymorphism in the acidic chitinase locus (ChiA) region of the wild plant Arabidopsis thaliana." Kawabe A., Innan H., Terauchi R., Miyashita N.T. Mol. Biol. Evol. 14:1303-1315(1997) [PubMed: 9402740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], POLYMORPHISM. Strain: cv. Al-0, cv. Bl-1, cv. Bla-10, cv. Bs-1, cv. Chi-0, cv. Ci-0, cv. Es-0, cv. Gr-1, cv. Hiroshima, cv. Ita-0, cv. Kn-0, cv. Mt-0, cv. Pog-0, cv. Shokei and cv. Yo-0.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones." Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:41-54(1998) [PubMed: 9628582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M34107 Genomic DNA. Translation: AAA32768.1. AB006055 Genomic DNA. Translation: BAA21860.1. AB006056 Genomic DNA. Translation: BAA21861.1. AB006057 Genomic DNA. Translation: BAA21862.1. AB006058 Genomic DNA. Translation: BAA21863.1. AB006059 Genomic DNA. Translation: BAA21864.1. AB006060 Genomic DNA. Translation: BAA21865.1. AB006061 Genomic DNA. Translation: BAA21866.1. AB006062 Genomic DNA. Translation: BAA21867.1. AB006063 Genomic DNA. Translation: BAA21868.1. AB006064 Genomic DNA. Translation: BAA21869.1. AB006065 Genomic DNA. Translation: BAA21870.1. AB006066 Genomic DNA. Translation: BAA21871.1. AB006067 Genomic DNA. Translation: BAA21872.1. AB006068 Genomic DNA. Translation: BAA21873.1. AB006069 Genomic DNA. Translation: BAA21874.1. AB009056 Genomic DNA. Translation: BAB08732.1. BT020526 mRNA. Translation: AAW49295.1. BT021931 mRNA. Translation: AAX49380.1.
IPI	IPI00546917.
PIR	A45511.
RefSeq	NP_197797.1.
UniGene	At.43218
3D structure databases
HSSP	HSSP built from PDB template 2HVM based on UniProtKB P23472.
SMR	P19172. Positions 30-302.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Proteomic databases
PRIDE	P19172.
Genome annotation databases
GeneID	832474.
GenomeReviews	Gene locus AT5G24090 in contig BA000015_GR.
KEGG	ath:AT5G24090.
NMPDR	fig|3702.1.peg.24591.
Organism-specific databases
TAIR	At5g24090.
Enzyme and pathway databases
BRENDA	3.2.1.14. 302.
Gene expression databases
ArrayExpress	P19172.
Genevestigator	P19172.
GermOnline	AT5G24090. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
PROSITE	PS01095. CHITINASE_18. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_ARATH

Accession

Primary (citable) accession number: P19172
Secondary accession number(s): O22065 , O22066, O22067, O22068, O22069, O22070, O22071, O22072, O22073, O24614, Q5HYZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 10, 2003
Last modified:	October 13, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)