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P19171

- CHIB_ARATH

UniProt

P19171 - CHIB_ARATH

Protein

Basic endochitinase B

Gene

CHI-B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 3 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Defense against chitin containing fungal pathogens. Seems particularly implicated in resistance to jasmonate-inducing pathogens such as A.brassicicola. In vitro antifungal activity against T.reesei, but not against A.solani, F.oxysporum, S.sclerotiorum, G.graminis and P.megasperma.1 Publication

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB-EC
    2. chitin binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. chitin catabolic process Source: UniProtKB-KW
    3. defense response to fungus Source: UniProtKB-KW
    4. killing of cells of other organism Source: UniProtKB-KW
    5. plant-type hypersensitive response Source: UniProtKB-KW
    6. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antimicrobial, Fungicide, Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Hypersensitive response, Plant defense, Polysaccharide degradation

    Keywords - Ligandi

    Chitin-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G12500-MONOMER.

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.
    GH19. Glycoside Hydrolase Family 19.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Basic endochitinase B (EC:3.2.1.14)
    Alternative name(s):
    Pathogenesis-related protein 3
    Short name:
    AtChiB
    Short name:
    PR-3
    Gene namesi
    Name:CHI-B
    Synonyms:PR3
    Ordered Locus Names:At3g12500
    ORF Names:MQC3.32, T2E22.18, T2E22_119
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G12500.

    Subcellular locationi

    Vacuole 1 Publication

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3333Add
    BLAST
    Chaini34 – 328295Basic endochitinase BPRO_0000005287Add
    BLAST
    Propeptidei329 – 3357Removed in mature formCuratedPRO_0000005288

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi36 ↔ 51PROSITE-ProRule annotation
    Disulfide bondi45 ↔ 57PROSITE-ProRule annotation
    Disulfide bondi50 ↔ 64PROSITE-ProRule annotation
    Disulfide bondi69 ↔ 73PROSITE-ProRule annotation
    Disulfide bondi107 ↔ 169PROSITE-ProRule annotation
    Disulfide bondi181 ↔ 189PROSITE-ProRule annotation
    Disulfide bondi288 ↔ 320PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP19171.
    PRIDEiP19171.

    Expressioni

    Tissue specificityi

    High constitutive level in roots with lower levels in leaves and flowering shoots.1 Publication

    Inductioni

    Ethylene induces high levels of systemic expression of basic chitinase with expression increasing with plant age. Locally and systemically induced by jasmonic acid (JA) and pathogens such as A.brassicicola and P.syringae, particularly in case of hypersensitive responses (HR). Not induced by wounding.4 Publications

    Gene expression databases

    GenevestigatoriP19171.

    Interactioni

    Protein-protein interaction databases

    BioGridi5763. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP19171.
    SMRiP19171. Positions 34-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 7542Chitin-binding type-1PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi329 – 3357Vacuolar targeting signalSequence Analysis

    Sequence similaritiesi

    Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3979.
    HOGENOMiHOG000231411.
    InParanoidiP19171.
    KOiK01183.
    OMAiCHAVIAG.
    PhylomeDBiP19171.

    Family and domain databases

    Gene3Di3.30.60.10. 1 hit.
    InterProiIPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PfamiPF00187. Chitin_bind_1. 1 hit.
    PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001060. Endochitinase. 1 hit.
    PRINTSiPR00451. CHITINBINDNG.
    ProDomiPD000609. Chitin_bd_1. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00270. ChtBD1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19171-1 [UniParc]FASTAAdd to Basket

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    MPPQKENHRT LNKMKTNLFL FLIFSLLLSL SSAEQCGRQA GGALCPNGLC    50
    CSEFGWCGNT EPYCKQPGCQ SQCTPGGTPP GPTGDLSGII SSSQFDDMLK 100
    HRNDAACPAR GFYTYNAFIT AAKSFPGFGT TGDTATRKKE VAAFFGQTSH 150
    ETTGGWATAP DGPYSWGYCF KQEQNPASDY CEPSATWPCA SGKRYYGRGP 200
    MQLSWNYNYG LCGRAIGVDL LNNPDLVAND AVIAFKAAIW FWMTAQPPKP 250
    SCHAVIAGQW QPSDADRAAG RLPGYGVITN IINGGLECGR GQDGRVADRI 300
    GFYQRYCNIF GVNPGGNLDC YNQRSFVNGL LEAAI 335
    Length:335
    Mass (Da):36,184
    Last modified:August 30, 2005 - v3
    Checksum:i0973CE89B30ABF61
    GO

    Sequence cautioni

    The sequence AAK96819.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
    The sequence BAB03157.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti17 – 171N → K in strain: cv. Bl-1, cv. Shokei and cv. Yo-0.
    Natural varianti106 – 1061A → T in strain: cv. Aa-0.
    Natural varianti127 – 1271G → S in strain: cv. Yo-0.
    Natural varianti206 – 2061N → D in strain: cv. Ci-0.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38240 Genomic DNA. Translation: AAA32769.1.
    AB023448 Genomic DNA. Translation: BAA82810.1.
    AB023449 Genomic DNA. Translation: BAA82811.1.
    AB023450 Genomic DNA. Translation: BAA82812.1.
    AB023451 Genomic DNA. Translation: BAA82813.1.
    AB023452 Genomic DNA. Translation: BAA82814.1.
    AB023453 Genomic DNA. Translation: BAA82815.1.
    AB023454 Genomic DNA. Translation: BAA82816.1.
    AB023455 Genomic DNA. Translation: BAA82817.1.
    AB023456 Genomic DNA. Translation: BAA82818.1.
    AB023457 Genomic DNA. Translation: BAA82819.1.
    AB023458 Genomic DNA. Translation: BAA82820.1.
    AB023459 Genomic DNA. Translation: BAA82821.1.
    AB023460 Genomic DNA. Translation: BAA82822.1.
    AB023461 Genomic DNA. Translation: BAA82823.1.
    AB023462 Genomic DNA. Translation: BAA82824.1.
    AB023463 Genomic DNA. Translation: BAA82825.1.
    AP002047 Genomic DNA. Translation: BAB03157.1. Different initiation.
    AC069474 Genomic DNA. Translation: AAG51023.1.
    CP002686 Genomic DNA. Translation: AEE75203.1.
    AY054628 mRNA. Translation: AAK96819.1. Different initiation.
    AY081519 mRNA. Translation: AAM10081.1.
    PIRiB45511.
    RefSeqiNP_566426.2. NM_112085.3.
    UniGeneiAt.296.
    At.75583.

    Genome annotation databases

    EnsemblPlantsiAT3G12500.1; AT3G12500.1; AT3G12500.
    GeneIDi820429.
    KEGGiath:AT3G12500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M38240 Genomic DNA. Translation: AAA32769.1 .
    AB023448 Genomic DNA. Translation: BAA82810.1 .
    AB023449 Genomic DNA. Translation: BAA82811.1 .
    AB023450 Genomic DNA. Translation: BAA82812.1 .
    AB023451 Genomic DNA. Translation: BAA82813.1 .
    AB023452 Genomic DNA. Translation: BAA82814.1 .
    AB023453 Genomic DNA. Translation: BAA82815.1 .
    AB023454 Genomic DNA. Translation: BAA82816.1 .
    AB023455 Genomic DNA. Translation: BAA82817.1 .
    AB023456 Genomic DNA. Translation: BAA82818.1 .
    AB023457 Genomic DNA. Translation: BAA82819.1 .
    AB023458 Genomic DNA. Translation: BAA82820.1 .
    AB023459 Genomic DNA. Translation: BAA82821.1 .
    AB023460 Genomic DNA. Translation: BAA82822.1 .
    AB023461 Genomic DNA. Translation: BAA82823.1 .
    AB023462 Genomic DNA. Translation: BAA82824.1 .
    AB023463 Genomic DNA. Translation: BAA82825.1 .
    AP002047 Genomic DNA. Translation: BAB03157.1 . Different initiation.
    AC069474 Genomic DNA. Translation: AAG51023.1 .
    CP002686 Genomic DNA. Translation: AEE75203.1 .
    AY054628 mRNA. Translation: AAK96819.1 . Different initiation.
    AY081519 mRNA. Translation: AAM10081.1 .
    PIRi B45511.
    RefSeqi NP_566426.2. NM_112085.3.
    UniGenei At.296.
    At.75583.

    3D structure databases

    ProteinModelPortali P19171.
    SMRi P19171. Positions 34-326.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 5763. 1 interaction.

    Protein family/group databases

    CAZyi CBM18. Carbohydrate-Binding Module Family 18.
    GH19. Glycoside Hydrolase Family 19.

    Proteomic databases

    PaxDbi P19171.
    PRIDEi P19171.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G12500.1 ; AT3G12500.1 ; AT3G12500 .
    GeneIDi 820429.
    KEGGi ath:AT3G12500.

    Organism-specific databases

    TAIRi AT3G12500.

    Phylogenomic databases

    eggNOGi COG3979.
    HOGENOMi HOG000231411.
    InParanoidi P19171.
    KOi K01183.
    OMAi CHAVIAG.
    PhylomeDBi P19171.

    Enzyme and pathway databases

    BioCyci ARA:AT3G12500-MONOMER.

    Gene expression databases

    Genevestigatori P19171.

    Family and domain databases

    Gene3Di 3.30.60.10. 1 hit.
    InterProi IPR001002. Chitin-bd_1.
    IPR018371. Chitin-binding_1_CS.
    IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    Pfami PF00187. Chitin_bind_1. 1 hit.
    PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001060. Endochitinase. 1 hit.
    PRINTSi PR00451. CHITINBINDNG.
    ProDomi PD000609. Chitin_bd_1. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00270. ChtBD1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    SSF57016. SSF57016. 1 hit.
    PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
    PS50941. CHIT_BIND_I_2. 1 hit.
    PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the genes encoding basic and acidic chitinase in Arabidopsis thaliana."
      Samac D.A., Hironaka C.M., Yallaly P.E., Shah D.M.
      Plant Physiol. 93:907-914(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Columbia.
    2. "DNA variation in the basic chitinase locus (ChiB) region of the wild plant Arabidopsis thaliana."
      Kawabe A., Miyashita N.T.
      Genetics 153:1445-1453(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS.
      Strain: cv. Aa-0, cv. Al-0, cv. Bl-1, cv. Bla-10, cv. Bs-1, cv. Chi-0, cv. Ci-0, cv. Es-0, cv. Gr-1, cv. Hiroshima, cv. Ita-0, cv. Kn-0, cv. Mt-0, cv. Pog-0, cv. Shokei and cv. Yo-0.
    3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-335.
      Strain: cv. Columbia.
    7. "Purification and characterization of an antifungal chitinase from Arabidopsis thaliana."
      Verburg J.G., Huynh Q.K.
      Plant Physiol. 95:450-455(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 237-256, ANTIFUNGAL ACTIVITY.
    8. "Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant Arabidopsis."
      Chen Q.G., Bleecker A.B.
      Plant Physiol. 108:597-607(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens."
      Thomma B.P.H.J., Eggermont K., Penninckx I.A.M.A., Mauch-Mani B., Vogelsang R., Cammue B.P.A., Broekaert W.F.
      Proc. Natl. Acad. Sci. U.S.A. 95:15107-15111(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    10. "Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis is not associated with a direct effect on expression of known defense-related genes but stimulates the expression of the jasmonate-inducible gene Atvsp upon challenge."
      van Wees S.C., Luijendijk M., Smoorenburg I., van Loon L.C., Pieterse C.M.
      Plant Mol. Biol. 41:537-549(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    11. "An in vivo expression system for the identification of cargo proteins of vacuolar sorting receptors in Arabidopsis culture cells."
      Shen J., Suen P.K., Wang X., Lin Y., Lo S.W., Rojo E., Jiang L.
      Plant J. 75:1003-1017(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCHIB_ARATH
    AccessioniPrimary (citable) accession number: P19171
    Secondary accession number(s): Q9S7J5
    , Q9S838, Q9SXJ2, Q9SXJ3, Q9SXJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 147 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3