Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Basic endochitinase B

Gene

CHI-B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Defense against chitin-containing fungal pathogens. Seems particularly implicated in resistance to jasmonate-inducing pathogens such as A.brassicicola. In vitro antifungal activity against T.reesei, but not against A.solani, F.oxysporum, S.sclerotiorum, G.graminis and P.megasperma.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Fungicide, Glycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Hypersensitive response, Plant defense, Polysaccharide degradation
LigandChitin-binding

Enzyme and pathway databases

BioCyciARA:AT3G12500-MONOMER

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Basic endochitinase B (EC:3.2.1.14)
Alternative name(s):
Pathogenesis-related protein 3
Short name:
AtChiB
Short name:
PR-3
Gene namesi
Name:CHI-B
Synonyms:PR3
Ordered Locus Names:At3g12500
ORF Names:MQC3.32, T2E22.18, T2E22_119
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G12500
TAIRilocus:2092502 AT3G12500

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Add BLAST33
ChainiPRO_000000528734 – 328Basic endochitinase BAdd BLAST295
PropeptideiPRO_0000005288329 – 335Removed in mature formCurated7

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 51PROSITE-ProRule annotation
Disulfide bondi45 ↔ 57PROSITE-ProRule annotation
Disulfide bondi50 ↔ 64PROSITE-ProRule annotation
Disulfide bondi69 ↔ 73PROSITE-ProRule annotation
Disulfide bondi107 ↔ 169PROSITE-ProRule annotation
Disulfide bondi181 ↔ 189PROSITE-ProRule annotation
Disulfide bondi288 ↔ 320PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19171
PRIDEiP19171

Expressioni

Tissue specificityi

High constitutive level in roots with lower levels in leaves and flowering shoots.1 Publication

Inductioni

Ethylene induces high levels of systemic expression of basic chitinase with expression increasing with plant age. Locally and systemically induced by jasmonic acid (JA) and pathogens such as A.brassicicola and P.syringae, particularly in case of hypersensitive responses (HR). Not induced by wounding.4 Publications

Gene expression databases

ExpressionAtlasiP19171 baseline and differential
GenevisibleiP19171 AT

Interactioni

Protein-protein interaction databases

BioGridi5763, 1 interactor
STRINGi3702.AT3G12500.1

Structurei

3D structure databases

ProteinModelPortaliP19171
SMRiP19171
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 75Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST42

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi329 – 335Vacuolar targeting signalSequence analysis7

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742 Eukaryota
COG3979 LUCA
HOGENOMiHOG000231411
InParanoidiP19171
KOiK20547
OMAiATWPCAS
OrthoDBiEOG09360IMR
PhylomeDBiP19171

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 1 hit
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit
PS00774 CHITINASE_19_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPQKENHRT LNKMKTNLFL FLIFSLLLSL SSAEQCGRQA GGALCPNGLC
60 70 80 90 100
CSEFGWCGNT EPYCKQPGCQ SQCTPGGTPP GPTGDLSGII SSSQFDDMLK
110 120 130 140 150
HRNDAACPAR GFYTYNAFIT AAKSFPGFGT TGDTATRKKE VAAFFGQTSH
160 170 180 190 200
ETTGGWATAP DGPYSWGYCF KQEQNPASDY CEPSATWPCA SGKRYYGRGP
210 220 230 240 250
MQLSWNYNYG LCGRAIGVDL LNNPDLVAND AVIAFKAAIW FWMTAQPPKP
260 270 280 290 300
SCHAVIAGQW QPSDADRAAG RLPGYGVITN IINGGLECGR GQDGRVADRI
310 320 330
GFYQRYCNIF GVNPGGNLDC YNQRSFVNGL LEAAI
Length:335
Mass (Da):36,184
Last modified:August 30, 2005 - v3
Checksum:i0973CE89B30ABF61
GO

Sequence cautioni

The sequence AAK96819 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB03157 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17N → K in strain: cv. Bl-1, cv. Shokei and cv. Yo-0. 1
Natural varianti106A → T in strain: cv. Aa-0. 1
Natural varianti127G → S in strain: cv. Yo-0. 1
Natural varianti206N → D in strain: cv. Ci-0. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38240 Genomic DNA Translation: AAA32769.1
AB023448 Genomic DNA Translation: BAA82810.1
AB023449 Genomic DNA Translation: BAA82811.1
AB023450 Genomic DNA Translation: BAA82812.1
AB023451 Genomic DNA Translation: BAA82813.1
AB023452 Genomic DNA Translation: BAA82814.1
AB023453 Genomic DNA Translation: BAA82815.1
AB023454 Genomic DNA Translation: BAA82816.1
AB023455 Genomic DNA Translation: BAA82817.1
AB023456 Genomic DNA Translation: BAA82818.1
AB023457 Genomic DNA Translation: BAA82819.1
AB023458 Genomic DNA Translation: BAA82820.1
AB023459 Genomic DNA Translation: BAA82821.1
AB023460 Genomic DNA Translation: BAA82822.1
AB023461 Genomic DNA Translation: BAA82823.1
AB023462 Genomic DNA Translation: BAA82824.1
AB023463 Genomic DNA Translation: BAA82825.1
AP002047 Genomic DNA Translation: BAB03157.1 Different initiation.
AC069474 Genomic DNA Translation: AAG51023.1
CP002686 Genomic DNA Translation: AEE75203.1
AY054628 mRNA Translation: AAK96819.1 Different initiation.
AY081519 mRNA Translation: AAM10081.1
PIRiB45511
RefSeqiNP_566426.2, NM_112085.4
UniGeneiAt.296
At.75583

Genome annotation databases

EnsemblPlantsiAT3G12500.1; AT3G12500.1; AT3G12500
GeneIDi820429
GrameneiAT3G12500.1; AT3G12500.1; AT3G12500
KEGGiath:AT3G12500

Similar proteinsi

Entry informationi

Entry nameiCHIB_ARATH
AccessioniPrimary (citable) accession number: P19171
Secondary accession number(s): Q9S7J5
, Q9S838, Q9SXJ2, Q9SXJ3, Q9SXJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 30, 2005
Last modified: April 25, 2018
This is version 171 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health