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P19171

- CHIB_ARATH

UniProt

P19171 - CHIB_ARATH

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Protein

Basic endochitinase B

Gene

CHI-B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Defense against chitin containing fungal pathogens. Seems particularly implicated in resistance to jasmonate-inducing pathogens such as A.brassicicola. In vitro antifungal activity against T.reesei, but not against A.solani, F.oxysporum, S.sclerotiorum, G.graminis and P.megasperma.1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC
  2. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response to fungus Source: UniProtKB-KW
  4. killing of cells of other organism Source: UniProtKB-KW
  5. plant-type hypersensitive response Source: UniProtKB-KW
  6. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide, Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Hypersensitive response, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Enzyme and pathway databases

BioCyciARA:AT3G12500-MONOMER.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Basic endochitinase B (EC:3.2.1.14)
Alternative name(s):
Pathogenesis-related protein 3
Short name:
AtChiB
Short name:
PR-3
Gene namesi
Name:CHI-B
Synonyms:PR3
Ordered Locus Names:At3g12500
ORF Names:MQC3.32, T2E22.18, T2E22_119
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G12500.

Subcellular locationi

Vacuole 1 Publication

GO - Cellular componenti

  1. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Add
BLAST
Chaini34 – 328295Basic endochitinase BPRO_0000005287Add
BLAST
Propeptidei329 – 3357Removed in mature formCuratedPRO_0000005288

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 51PROSITE-ProRule annotation
Disulfide bondi45 ↔ 57PROSITE-ProRule annotation
Disulfide bondi50 ↔ 64PROSITE-ProRule annotation
Disulfide bondi69 ↔ 73PROSITE-ProRule annotation
Disulfide bondi107 ↔ 169PROSITE-ProRule annotation
Disulfide bondi181 ↔ 189PROSITE-ProRule annotation
Disulfide bondi288 ↔ 320PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP19171.
PRIDEiP19171.

Expressioni

Tissue specificityi

High constitutive level in roots with lower levels in leaves and flowering shoots.1 Publication

Inductioni

Ethylene induces high levels of systemic expression of basic chitinase with expression increasing with plant age. Locally and systemically induced by jasmonic acid (JA) and pathogens such as A.brassicicola and P.syringae, particularly in case of hypersensitive responses (HR). Not induced by wounding.4 Publications

Gene expression databases

GenevestigatoriP19171.

Interactioni

Protein-protein interaction databases

BioGridi5763. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP19171.
SMRiP19171. Positions 34-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 7542Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi329 – 3357Vacuolar targeting signalSequence Analysis

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3979.
HOGENOMiHOG000231411.
InParanoidiP19171.
KOiK01183.
OMAiCHAVIAG.
PhylomeDBiP19171.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19171-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPQKENHRT LNKMKTNLFL FLIFSLLLSL SSAEQCGRQA GGALCPNGLC
60 70 80 90 100
CSEFGWCGNT EPYCKQPGCQ SQCTPGGTPP GPTGDLSGII SSSQFDDMLK
110 120 130 140 150
HRNDAACPAR GFYTYNAFIT AAKSFPGFGT TGDTATRKKE VAAFFGQTSH
160 170 180 190 200
ETTGGWATAP DGPYSWGYCF KQEQNPASDY CEPSATWPCA SGKRYYGRGP
210 220 230 240 250
MQLSWNYNYG LCGRAIGVDL LNNPDLVAND AVIAFKAAIW FWMTAQPPKP
260 270 280 290 300
SCHAVIAGQW QPSDADRAAG RLPGYGVITN IINGGLECGR GQDGRVADRI
310 320 330
GFYQRYCNIF GVNPGGNLDC YNQRSFVNGL LEAAI
Length:335
Mass (Da):36,184
Last modified:August 30, 2005 - v3
Checksum:i0973CE89B30ABF61
GO

Sequence cautioni

The sequence AAK96819.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence BAB03157.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171N → K in strain: cv. Bl-1, cv. Shokei and cv. Yo-0.
Natural varianti106 – 1061A → T in strain: cv. Aa-0.
Natural varianti127 – 1271G → S in strain: cv. Yo-0.
Natural varianti206 – 2061N → D in strain: cv. Ci-0.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38240 Genomic DNA. Translation: AAA32769.1.
AB023448 Genomic DNA. Translation: BAA82810.1.
AB023449 Genomic DNA. Translation: BAA82811.1.
AB023450 Genomic DNA. Translation: BAA82812.1.
AB023451 Genomic DNA. Translation: BAA82813.1.
AB023452 Genomic DNA. Translation: BAA82814.1.
AB023453 Genomic DNA. Translation: BAA82815.1.
AB023454 Genomic DNA. Translation: BAA82816.1.
AB023455 Genomic DNA. Translation: BAA82817.1.
AB023456 Genomic DNA. Translation: BAA82818.1.
AB023457 Genomic DNA. Translation: BAA82819.1.
AB023458 Genomic DNA. Translation: BAA82820.1.
AB023459 Genomic DNA. Translation: BAA82821.1.
AB023460 Genomic DNA. Translation: BAA82822.1.
AB023461 Genomic DNA. Translation: BAA82823.1.
AB023462 Genomic DNA. Translation: BAA82824.1.
AB023463 Genomic DNA. Translation: BAA82825.1.
AP002047 Genomic DNA. Translation: BAB03157.1. Different initiation.
AC069474 Genomic DNA. Translation: AAG51023.1.
CP002686 Genomic DNA. Translation: AEE75203.1.
AY054628 mRNA. Translation: AAK96819.1. Different initiation.
AY081519 mRNA. Translation: AAM10081.1.
PIRiB45511.
RefSeqiNP_566426.2. NM_112085.3.
UniGeneiAt.296.
At.75583.

Genome annotation databases

EnsemblPlantsiAT3G12500.1; AT3G12500.1; AT3G12500.
GeneIDi820429.
KEGGiath:AT3G12500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38240 Genomic DNA. Translation: AAA32769.1 .
AB023448 Genomic DNA. Translation: BAA82810.1 .
AB023449 Genomic DNA. Translation: BAA82811.1 .
AB023450 Genomic DNA. Translation: BAA82812.1 .
AB023451 Genomic DNA. Translation: BAA82813.1 .
AB023452 Genomic DNA. Translation: BAA82814.1 .
AB023453 Genomic DNA. Translation: BAA82815.1 .
AB023454 Genomic DNA. Translation: BAA82816.1 .
AB023455 Genomic DNA. Translation: BAA82817.1 .
AB023456 Genomic DNA. Translation: BAA82818.1 .
AB023457 Genomic DNA. Translation: BAA82819.1 .
AB023458 Genomic DNA. Translation: BAA82820.1 .
AB023459 Genomic DNA. Translation: BAA82821.1 .
AB023460 Genomic DNA. Translation: BAA82822.1 .
AB023461 Genomic DNA. Translation: BAA82823.1 .
AB023462 Genomic DNA. Translation: BAA82824.1 .
AB023463 Genomic DNA. Translation: BAA82825.1 .
AP002047 Genomic DNA. Translation: BAB03157.1 . Different initiation.
AC069474 Genomic DNA. Translation: AAG51023.1 .
CP002686 Genomic DNA. Translation: AEE75203.1 .
AY054628 mRNA. Translation: AAK96819.1 . Different initiation.
AY081519 mRNA. Translation: AAM10081.1 .
PIRi B45511.
RefSeqi NP_566426.2. NM_112085.3.
UniGenei At.296.
At.75583.

3D structure databases

ProteinModelPortali P19171.
SMRi P19171. Positions 34-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 5763. 1 interaction.

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbi P19171.
PRIDEi P19171.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G12500.1 ; AT3G12500.1 ; AT3G12500 .
GeneIDi 820429.
KEGGi ath:AT3G12500.

Organism-specific databases

TAIRi AT3G12500.

Phylogenomic databases

eggNOGi COG3979.
HOGENOMi HOG000231411.
InParanoidi P19171.
KOi K01183.
OMAi CHAVIAG.
PhylomeDBi P19171.

Enzyme and pathway databases

BioCyci ARA:AT3G12500-MONOMER.

Gene expression databases

Genevestigatori P19171.

Family and domain databases

Gene3Di 3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
PRINTSi PR00451. CHITINBINDNG.
ProDomi PD000609. Chitin_bd_1. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of the genes encoding basic and acidic chitinase in Arabidopsis thaliana."
    Samac D.A., Hironaka C.M., Yallaly P.E., Shah D.M.
    Plant Physiol. 93:907-914(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Columbia.
  2. "DNA variation in the basic chitinase locus (ChiB) region of the wild plant Arabidopsis thaliana."
    Kawabe A., Miyashita N.T.
    Genetics 153:1445-1453(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS.
    Strain: cv. Aa-0, cv. Al-0, cv. Bl-1, cv. Bla-10, cv. Bs-1, cv. Chi-0, cv. Ci-0, cv. Es-0, cv. Gr-1, cv. Hiroshima, cv. Ita-0, cv. Kn-0, cv. Mt-0, cv. Pog-0, cv. Shokei and cv. Yo-0.
  3. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-335.
    Strain: cv. Columbia.
  7. "Purification and characterization of an antifungal chitinase from Arabidopsis thaliana."
    Verburg J.G., Huynh Q.K.
    Plant Physiol. 95:450-455(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 237-256, ANTIFUNGAL ACTIVITY.
  8. "Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant Arabidopsis."
    Chen Q.G., Bleecker A.B.
    Plant Physiol. 108:597-607(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  9. "Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens."
    Thomma B.P.H.J., Eggermont K., Penninckx I.A.M.A., Mauch-Mani B., Vogelsang R., Cammue B.P.A., Broekaert W.F.
    Proc. Natl. Acad. Sci. U.S.A. 95:15107-15111(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  10. "Rhizobacteria-mediated induced systemic resistance (ISR) in Arabidopsis is not associated with a direct effect on expression of known defense-related genes but stimulates the expression of the jasmonate-inducible gene Atvsp upon challenge."
    van Wees S.C., Luijendijk M., Smoorenburg I., van Loon L.C., Pieterse C.M.
    Plant Mol. Biol. 41:537-549(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "An in vivo expression system for the identification of cargo proteins of vacuolar sorting receptors in Arabidopsis culture cells."
    Shen J., Suen P.K., Wang X., Lin Y., Lo S.W., Rojo E., Jiang L.
    Plant J. 75:1003-1017(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCHIB_ARATH
AccessioniPrimary (citable) accession number: P19171
Secondary accession number(s): Q9S7J5
, Q9S838, Q9SXJ2, Q9SXJ3, Q9SXJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 30, 2005
Last modified: October 29, 2014
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3