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P19158

- IRA2_YEAST

UniProt

P19158 - IRA2_YEAST

Protein

Inhibitory regulator protein IRA2

Gene

IRA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase activity of Ras proteins.

    GO - Molecular functioni

    1. Ras GTPase activator activity Source: SGD

    GO - Biological processi

    1. cellular response to cold Source: SGD
    2. cellular response to hydrogen peroxide Source: SGD
    3. cellular response to metal ion Source: SGD
    4. cellular response to oxidative stress Source: SGD
    5. negative regulation of cAMP biosynthetic process Source: SGD
    6. negative regulation of Ras protein signal transduction Source: SGD
    7. positive regulation of Ras GTPase activity Source: SGD
    8. signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33484-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inhibitory regulator protein IRA2
    Gene namesi
    Name:IRA2
    Synonyms:CCS1, GLC4
    Ordered Locus Names:YOL081W
    ORF Names:O0985
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL081w.
    SGDiS000005441. IRA2.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 30793079Inhibitory regulator protein IRA2PRO_0000056658Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei635 – 6351Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP19158.
    PaxDbiP19158.
    PeptideAtlasiP19158.

    Expressioni

    Gene expression databases

    GenevestigatoriP19158.

    Interactioni

    Protein-protein interaction databases

    BioGridi34321. 573 interactions.
    DIPiDIP-6376N.
    IntActiP19158. 2 interactions.
    MINTiMINT-597224.

    Structurei

    3D structure databases

    ProteinModelPortaliP19158.
    SMRiP19158. Positions 1706-1959.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1701 – 1890190Ras-GAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi399 – 40911Poly-SerAdd
    BLAST
    Compositional biasi412 – 4165Poly-Ser
    Compositional biasi520 – 5289Poly-Ala
    Compositional biasi2469 – 24724Poly-Leu

    Sequence similaritiesi

    Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG255236.
    GeneTreeiENSGT00550000074797.
    HOGENOMiHOG000113101.
    OMAiFQVLHET.
    OrthoDBiEOG7H1JTR.

    Family and domain databases

    Gene3Di1.10.506.10. 1 hit.
    InterProiIPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view]
    PfamiPF00616. RasGAP. 1 hit.
    [Graphical view]
    SMARTiSM00323. RasGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P19158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQPTKNKKK EHGTDSKSSR MTRTLVNHIL FERILPILPV ESNLSTYSEV     50
    EEYSSFISCR SVLINVTVSR DANAMVEGTL ELIESLLQGH EIISDKGSSD 100
    VIESILIILR LLSDALEYNW QNQESLHYND ISTHVEHDQE QKYRPKLNSI 150
    LPDYSSTHSN GNKHFFHQSK PQALIPELAS KLLESCAKLK FNTRTLQILQ 200
    NMISHVHGNI LTTLSSSILP RHKSYLTRHN HPSHCKMIDS TLGHILRFVA 250
    ASNPSEYFEF IRKSVQVPVT QTHTHSHSHS HSLPSSVYNS IVPHFDLFSF 300
    IYLSKHNFKK YLELIKNLSV TLRKTIYHCL LLHYSAKAIM FWIMARPAEY 350
    YELFNLLKDN NNEHSKSLNT LNHTLFEEIH STFNVNSMIT TNQNAHQGSS 400
    SPSSSSPSSP PSSSSSDNNN QNIIAKSLSR QLSHHQSYIQ QQSERKLHSS 450
    WTTNSQSSTS LSSSTSNSTT TDFSTHTQPG EYDPSLPDTP TMSNITISAS 500
    SLLSQTPTPT TQLQQRLNSA AAAAAAAASP SNSTPTGYTA EQQSRASYDA 550
    HKTGHTGKDY DEHFLSVTRL DNVLELYTHF DDTEVLPHTS VLKFLTTLTM 600
    FDIDLFNELN ATSFKYIPDC TMHRPKERTS SFNNTAHETG SEKTSGIKHI 650
    TQGLKKLTSL PSSTKKTVKF VKMLLRNLNG NQAVSDVALL DTMRALLSFF 700
    TMTSAVFLVD RNLPSVLFAK RLIPIMGTNL SVGQDWNSKI NNSLMVCLKK 750
    NSTTFVQLQL IFFSSAIQFD HELLLARLSI DTMANNLNMQ KLCLYTEGFR 800
    IFFDIPSKKE LRKAIAVKIS KFFKTLFSII ADILLQEFPY FDEQITDIVA 850
    SILDGTIINE YGTKKHFKGS SPSLCSTTRS RSGSTSQSSM TPVSPLGLDT 900
    DICPMNTLSL VGSSTSRNSD NVNSLNSSPK NLSSDPYLSH LVAPRARHAL 950
    GGPSSIIRNK IPTTLTSPPG TEKSSPVQRP QTESISATPM AITNSTPLSS 1000
    AAFGIRSPLQ KIRTRRYSDE SLGKFMKSTN NYIQEHLIPK DLNEATLQDA 1050
    RRIMINIFSI FKRPNSYFII PHNINSNLQW VSQDFRNIMK PIFVAIVSPD 1100
    VDLQNTAQSF MDTLLSNVIT YGESDENISI EGYHLLCSYT VTLFAMGLFD 1150
    LKINNEKRQI LLDITVKFMK VRSHLAGIAE ASHHMEYISD SEKLTFPLIM 1200
    GTVGRALFVS LYSSQQKIEK TLKIAYTEYL SAINFHERNI DDADKTWVHN 1250
    IEFVEAMCHD NYTTSGSIAF QRRTRNNILR FATIPNAILL DSMRMIYKKW 1300
    HTYTHSKSLE KQERNDFRNF AGILASLSGI LFINKKILQE MYPYLLDTVS 1350
    ELKKNIDSFI SKQCQWLNYP DLLTRENSRD ILSVELHPLS FNLLFNNLRL 1400
    KLKELACSDL SIPENESSYV LLEQIIKMLR TILGRDDDNY VMMLFSTEIV 1450
    DLIDLLTDEI KKIPAYCPKY LKAIIQMTKM FSALQHSEVN LGVKNHFHVK 1500
    NKWLRQITDW FQVSIAREYD FENLSKPLKE MDLVKRDMDI LYIDTAIEAS 1550
    TAIAYLTRHT FLEIPPAASD PELSRSRSVI FGFYFNILMK GLEKSSDRDN 1600
    YPVFLRHKMS VLNDNVILSL TNLSNTNVDA SLQFTLPMGY SGNRNIRNAF 1650
    LEVFINIVTN YRTYTAKTDL GKLEAADKFL RYTIEHPQLS SFGAAVCPAS 1700
    DIDAYAAGLI NAFETRNATH IVVAQLIKNE IEKSSRPTDI LRRNSCATRS 1750
    LSMLARSKGN EYLIRTLQPL LKKIIQNRDF FEIEKLKPED SDAERQIELF 1800
    VKYMNELLES ISNSVSYFPP PLFYICQNIY KVACEKFPDH AIIAAGSFVF 1850
    LRFFCPALVS PDSENIIDIS HLSEKRTFIS LAKVIQNIAN GSENFSRWPA 1900
    LCSQKDFLKE CSDRIFRFLA ELCRTDRTID IQVRTDPTPI AFDYQFLHSF 1950
    VYLYGLEVRR NVLNEAKHDD GDIDGDDFYK TTFLLIDDVL GQLGQPKMEF 2000
    SNEIPIYIRE HMDDYPELYE FMNRHAFRNI ETSTAYSPSV HESTSSEGIP 2050
    IITLTMSNFS DRHVDIDTVA YKFLQIYARI WTTKHCLIID CTEFDEGGLD 2100
    MRKFISLVMG LLPEVAPKNC IGCYYFNVNE TFMDNYGKCL DKDNVYVSSK 2150
    IPHYFINSNS DEGLMKSVGI TGQGLKVLQD IRVSLHDITL YDEKRNRFTP 2200
    VSLKIGDIYF QVLHETPRQY KIRDMGTLFD VKFNDVYEIS RIFEVHVSSI 2250
    TGVAAEFTVT FQDERRLIFS SPKYLEIVKM FYYAQIRLES EYEMDNNSST 2300
    SSPNSNNKDK QQKERTKLLC HLLLVSLIGL FDESKKMKNS SYNLIAATEA 2350
    SFGLNFGSHF HRSPEVYVPE DTTTFLGVIG KSLAESNPEL TAYMFIYVLE 2400
    ALKNNVIPHV YIPHTICGLS YWIPNLYQHV YLADDEEGPE NISHIFRILI 2450
    RLSVRETDFK AVYMQYVWLL LLDDGRLTDI IVDEVINHAL ERDSENRDWK 2500
    KTISLLTVLP TTEVANNIIQ KILAKIRSFL PSLKLEAMTQ SWSELTILVK 2550
    ISIHVFFETS LLVQMYLPEI LFIVSLLIDV GPRELRSSLH QLLMNVCHSL 2600
    AINSALPQDH RNNLDEISDI FAHQKVKFMF GFSEDKGRIL QIFSASSFAS 2650
    KFNILDFFIN NILLLMEYSS TYEANVWKTR YKKYVLESVF TSNSFLSARS 2700
    IMIVGIMGKS YITEGLCKAM LIETMKVIAE PKITDEHLFL AISHIFTYSK 2750
    IVEGLDPNLD LMKHLFWFST LFLESRHPII FEGALLFVSN CIRRLYMAQF 2800
    ENESETSLIS TLLKGRKFAH TFLSKIENLS GIVWNEDNFT HILIFIINKG 2850
    LSNPFIKSTA FDFLKMMFRN SYFEHQINQK SDHYLCYMFL LYFVLNCNQF 2900
    EELLGDVDFE GEMVNIENKN TIPKILLEWL SSDNENANIT LYQGAILFKC 2950
    SVTDEPSRFR FALIIRHLLT KKPICALRFY SVIRNEIRKI SAFEQNSDCV 3000
    PLAFDILNLL VTHSESNSLE KLHEESIERL TKRGLSIVTS SGIFAKNSDM 3050
    MIPLDVKPED IYERKRIMTM ILSRMSCSA 3079
    Length:3,079
    Mass (Da):351,669
    Last modified:September 5, 2006 - v2
    Checksum:iC14B5396D6FB9B5C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2309 – 23091D → V in AAA34710. (PubMed:2164637)Curated
    Sequence conflicti2317 – 23171K → I in AAA34710. (PubMed:2164637)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33779 Genomic DNA. Translation: AAA34710.1.
    Z74823 Genomic DNA. Translation: CAA99093.1.
    X75449 Genomic DNA. Translation: CAA53202.1.
    X83121 Genomic DNA. Translation: CAA58201.1.
    Z74822 Genomic DNA. Translation: CAA99092.1.
    BK006948 Genomic DNA. Translation: DAA10703.1.
    PIRiS66775. RGBYI2.
    RefSeqiNP_014560.1. NM_001183335.1.

    Genome annotation databases

    EnsemblFungiiYOL081W; YOL081W; YOL081W.
    GeneIDi854073.
    KEGGisce:YOL081W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33779 Genomic DNA. Translation: AAA34710.1 .
    Z74823 Genomic DNA. Translation: CAA99093.1 .
    X75449 Genomic DNA. Translation: CAA53202.1 .
    X83121 Genomic DNA. Translation: CAA58201.1 .
    Z74822 Genomic DNA. Translation: CAA99092.1 .
    BK006948 Genomic DNA. Translation: DAA10703.1 .
    PIRi S66775. RGBYI2.
    RefSeqi NP_014560.1. NM_001183335.1.

    3D structure databases

    ProteinModelPortali P19158.
    SMRi P19158. Positions 1706-1959.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34321. 573 interactions.
    DIPi DIP-6376N.
    IntActi P19158. 2 interactions.
    MINTi MINT-597224.

    Proteomic databases

    MaxQBi P19158.
    PaxDbi P19158.
    PeptideAtlasi P19158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL081W ; YOL081W ; YOL081W .
    GeneIDi 854073.
    KEGGi sce:YOL081W.

    Organism-specific databases

    CYGDi YOL081w.
    SGDi S000005441. IRA2.

    Phylogenomic databases

    eggNOGi NOG255236.
    GeneTreei ENSGT00550000074797.
    HOGENOMi HOG000113101.
    OMAi FQVLHET.
    OrthoDBi EOG7H1JTR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33484-MONOMER.

    Miscellaneous databases

    NextBioi 975695.

    Gene expression databases

    Genevestigatori P19158.

    Family and domain databases

    Gene3Di 1.10.506.10. 1 hit.
    InterProi IPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view ]
    Pfami PF00616. RasGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00323. RasGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein."
      Tanaka K., Nakafuku M., Tamanoi F., Kaziro Y., Matsumoto K., Toh-e A.
      Mol. Cell. Biol. 10:4303-4313(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Sequence of a 10.27 kb segment on the left arm of chromosome XV from Saccharomyces cerevisiae includes part of the IRA2 gene and a putative new gene."
      Zumstein E., Griffin H., Schweizer M.
      Yeast 10:1383-1387(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
      Strain: ATCC 96604 / S288c / FY1679.
    5. "A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames."
      Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.
      Yeast 11:975-986(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
      Strain: ATCC 96604 / S288c / FY1679.
    6. "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
      Tzermia M., Katsoulou C., Alexandraki D.
      Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1982-3079.
    7. "The CCS1 gene from Saccharomyces cerevisiae which is involved in mitochondrial functions is identified as IRA2 an attenuator of RAS1 and RAS2 gene products."
      Bussereau F., Dupont C.H., Boy-Marcotte E., Mallet L., Jacquet M.
      Curr. Genet. 21:325-329(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF CCS1 AS IRA2.
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIRA2_YEAST
    AccessioniPrimary (citable) accession number: P19158
    Secondary accession number(s): D6W1Y7, O13592, Q08239
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3