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P19158

- IRA2_YEAST

UniProt

P19158 - IRA2_YEAST

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Protein
Inhibitory regulator protein IRA2
Gene
IRA2, CCS1, GLC4, YOL081W, O0985
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase activity of Ras proteins.

GO - Molecular functioni

  1. Ras GTPase activator activity Source: SGD

GO - Biological processi

  1. cellular response to cold Source: SGD
  2. cellular response to hydrogen peroxide Source: SGD
  3. cellular response to metal ion Source: SGD
  4. cellular response to oxidative stress Source: SGD
  5. negative regulation of Ras protein signal transduction Source: SGD
  6. negative regulation of cAMP biosynthetic process Source: SGD
  7. positive regulation of Ras GTPase activity Source: SGD
  8. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-33484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory regulator protein IRA2
Gene namesi
Name:IRA2
Synonyms:CCS1, GLC4
Ordered Locus Names:YOL081W
ORF Names:O0985
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL081w.
SGDiS000005441. IRA2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30793079Inhibitory regulator protein IRA2
PRO_0000056658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei635 – 6351Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP19158.
PaxDbiP19158.
PeptideAtlasiP19158.

Expressioni

Gene expression databases

GenevestigatoriP19158.

Interactioni

Protein-protein interaction databases

BioGridi34321. 573 interactions.
DIPiDIP-6376N.
IntActiP19158. 2 interactions.
MINTiMINT-597224.

Structurei

3D structure databases

ProteinModelPortaliP19158.
SMRiP19158. Positions 1706-1959.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1701 – 1890190Ras-GAP
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi399 – 40911Poly-Ser
Add
BLAST
Compositional biasi412 – 4165Poly-Ser
Compositional biasi520 – 5289Poly-Ala
Compositional biasi2469 – 24724Poly-Leu

Sequence similaritiesi

Contains 1 Ras-GAP domain.

Phylogenomic databases

eggNOGiNOG255236.
GeneTreeiENSGT00550000074797.
HOGENOMiHOG000113101.
OMAiFQVLHET.
OrthoDBiEOG7H1JTR.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
InterProiIPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19158-1 [UniParc]FASTAAdd to Basket

« Hide

MSQPTKNKKK EHGTDSKSSR MTRTLVNHIL FERILPILPV ESNLSTYSEV     50
EEYSSFISCR SVLINVTVSR DANAMVEGTL ELIESLLQGH EIISDKGSSD 100
VIESILIILR LLSDALEYNW QNQESLHYND ISTHVEHDQE QKYRPKLNSI 150
LPDYSSTHSN GNKHFFHQSK PQALIPELAS KLLESCAKLK FNTRTLQILQ 200
NMISHVHGNI LTTLSSSILP RHKSYLTRHN HPSHCKMIDS TLGHILRFVA 250
ASNPSEYFEF IRKSVQVPVT QTHTHSHSHS HSLPSSVYNS IVPHFDLFSF 300
IYLSKHNFKK YLELIKNLSV TLRKTIYHCL LLHYSAKAIM FWIMARPAEY 350
YELFNLLKDN NNEHSKSLNT LNHTLFEEIH STFNVNSMIT TNQNAHQGSS 400
SPSSSSPSSP PSSSSSDNNN QNIIAKSLSR QLSHHQSYIQ QQSERKLHSS 450
WTTNSQSSTS LSSSTSNSTT TDFSTHTQPG EYDPSLPDTP TMSNITISAS 500
SLLSQTPTPT TQLQQRLNSA AAAAAAAASP SNSTPTGYTA EQQSRASYDA 550
HKTGHTGKDY DEHFLSVTRL DNVLELYTHF DDTEVLPHTS VLKFLTTLTM 600
FDIDLFNELN ATSFKYIPDC TMHRPKERTS SFNNTAHETG SEKTSGIKHI 650
TQGLKKLTSL PSSTKKTVKF VKMLLRNLNG NQAVSDVALL DTMRALLSFF 700
TMTSAVFLVD RNLPSVLFAK RLIPIMGTNL SVGQDWNSKI NNSLMVCLKK 750
NSTTFVQLQL IFFSSAIQFD HELLLARLSI DTMANNLNMQ KLCLYTEGFR 800
IFFDIPSKKE LRKAIAVKIS KFFKTLFSII ADILLQEFPY FDEQITDIVA 850
SILDGTIINE YGTKKHFKGS SPSLCSTTRS RSGSTSQSSM TPVSPLGLDT 900
DICPMNTLSL VGSSTSRNSD NVNSLNSSPK NLSSDPYLSH LVAPRARHAL 950
GGPSSIIRNK IPTTLTSPPG TEKSSPVQRP QTESISATPM AITNSTPLSS 1000
AAFGIRSPLQ KIRTRRYSDE SLGKFMKSTN NYIQEHLIPK DLNEATLQDA 1050
RRIMINIFSI FKRPNSYFII PHNINSNLQW VSQDFRNIMK PIFVAIVSPD 1100
VDLQNTAQSF MDTLLSNVIT YGESDENISI EGYHLLCSYT VTLFAMGLFD 1150
LKINNEKRQI LLDITVKFMK VRSHLAGIAE ASHHMEYISD SEKLTFPLIM 1200
GTVGRALFVS LYSSQQKIEK TLKIAYTEYL SAINFHERNI DDADKTWVHN 1250
IEFVEAMCHD NYTTSGSIAF QRRTRNNILR FATIPNAILL DSMRMIYKKW 1300
HTYTHSKSLE KQERNDFRNF AGILASLSGI LFINKKILQE MYPYLLDTVS 1350
ELKKNIDSFI SKQCQWLNYP DLLTRENSRD ILSVELHPLS FNLLFNNLRL 1400
KLKELACSDL SIPENESSYV LLEQIIKMLR TILGRDDDNY VMMLFSTEIV 1450
DLIDLLTDEI KKIPAYCPKY LKAIIQMTKM FSALQHSEVN LGVKNHFHVK 1500
NKWLRQITDW FQVSIAREYD FENLSKPLKE MDLVKRDMDI LYIDTAIEAS 1550
TAIAYLTRHT FLEIPPAASD PELSRSRSVI FGFYFNILMK GLEKSSDRDN 1600
YPVFLRHKMS VLNDNVILSL TNLSNTNVDA SLQFTLPMGY SGNRNIRNAF 1650
LEVFINIVTN YRTYTAKTDL GKLEAADKFL RYTIEHPQLS SFGAAVCPAS 1700
DIDAYAAGLI NAFETRNATH IVVAQLIKNE IEKSSRPTDI LRRNSCATRS 1750
LSMLARSKGN EYLIRTLQPL LKKIIQNRDF FEIEKLKPED SDAERQIELF 1800
VKYMNELLES ISNSVSYFPP PLFYICQNIY KVACEKFPDH AIIAAGSFVF 1850
LRFFCPALVS PDSENIIDIS HLSEKRTFIS LAKVIQNIAN GSENFSRWPA 1900
LCSQKDFLKE CSDRIFRFLA ELCRTDRTID IQVRTDPTPI AFDYQFLHSF 1950
VYLYGLEVRR NVLNEAKHDD GDIDGDDFYK TTFLLIDDVL GQLGQPKMEF 2000
SNEIPIYIRE HMDDYPELYE FMNRHAFRNI ETSTAYSPSV HESTSSEGIP 2050
IITLTMSNFS DRHVDIDTVA YKFLQIYARI WTTKHCLIID CTEFDEGGLD 2100
MRKFISLVMG LLPEVAPKNC IGCYYFNVNE TFMDNYGKCL DKDNVYVSSK 2150
IPHYFINSNS DEGLMKSVGI TGQGLKVLQD IRVSLHDITL YDEKRNRFTP 2200
VSLKIGDIYF QVLHETPRQY KIRDMGTLFD VKFNDVYEIS RIFEVHVSSI 2250
TGVAAEFTVT FQDERRLIFS SPKYLEIVKM FYYAQIRLES EYEMDNNSST 2300
SSPNSNNKDK QQKERTKLLC HLLLVSLIGL FDESKKMKNS SYNLIAATEA 2350
SFGLNFGSHF HRSPEVYVPE DTTTFLGVIG KSLAESNPEL TAYMFIYVLE 2400
ALKNNVIPHV YIPHTICGLS YWIPNLYQHV YLADDEEGPE NISHIFRILI 2450
RLSVRETDFK AVYMQYVWLL LLDDGRLTDI IVDEVINHAL ERDSENRDWK 2500
KTISLLTVLP TTEVANNIIQ KILAKIRSFL PSLKLEAMTQ SWSELTILVK 2550
ISIHVFFETS LLVQMYLPEI LFIVSLLIDV GPRELRSSLH QLLMNVCHSL 2600
AINSALPQDH RNNLDEISDI FAHQKVKFMF GFSEDKGRIL QIFSASSFAS 2650
KFNILDFFIN NILLLMEYSS TYEANVWKTR YKKYVLESVF TSNSFLSARS 2700
IMIVGIMGKS YITEGLCKAM LIETMKVIAE PKITDEHLFL AISHIFTYSK 2750
IVEGLDPNLD LMKHLFWFST LFLESRHPII FEGALLFVSN CIRRLYMAQF 2800
ENESETSLIS TLLKGRKFAH TFLSKIENLS GIVWNEDNFT HILIFIINKG 2850
LSNPFIKSTA FDFLKMMFRN SYFEHQINQK SDHYLCYMFL LYFVLNCNQF 2900
EELLGDVDFE GEMVNIENKN TIPKILLEWL SSDNENANIT LYQGAILFKC 2950
SVTDEPSRFR FALIIRHLLT KKPICALRFY SVIRNEIRKI SAFEQNSDCV 3000
PLAFDILNLL VTHSESNSLE KLHEESIERL TKRGLSIVTS SGIFAKNSDM 3050
MIPLDVKPED IYERKRIMTM ILSRMSCSA 3079
Length:3,079
Mass (Da):351,669
Last modified:September 5, 2006 - v2
Checksum:iC14B5396D6FB9B5C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2309 – 23091D → V in AAA34710. 1 Publication
Sequence conflicti2317 – 23171K → I in AAA34710. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33779 Genomic DNA. Translation: AAA34710.1.
Z74823 Genomic DNA. Translation: CAA99093.1.
X75449 Genomic DNA. Translation: CAA53202.1.
X83121 Genomic DNA. Translation: CAA58201.1.
Z74822 Genomic DNA. Translation: CAA99092.1.
BK006948 Genomic DNA. Translation: DAA10703.1.
PIRiS66775. RGBYI2.
RefSeqiNP_014560.1. NM_001183335.1.

Genome annotation databases

EnsemblFungiiYOL081W; YOL081W; YOL081W.
GeneIDi854073.
KEGGisce:YOL081W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33779 Genomic DNA. Translation: AAA34710.1 .
Z74823 Genomic DNA. Translation: CAA99093.1 .
X75449 Genomic DNA. Translation: CAA53202.1 .
X83121 Genomic DNA. Translation: CAA58201.1 .
Z74822 Genomic DNA. Translation: CAA99092.1 .
BK006948 Genomic DNA. Translation: DAA10703.1 .
PIRi S66775. RGBYI2.
RefSeqi NP_014560.1. NM_001183335.1.

3D structure databases

ProteinModelPortali P19158.
SMRi P19158. Positions 1706-1959.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34321. 573 interactions.
DIPi DIP-6376N.
IntActi P19158. 2 interactions.
MINTi MINT-597224.

Proteomic databases

MaxQBi P19158.
PaxDbi P19158.
PeptideAtlasi P19158.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL081W ; YOL081W ; YOL081W .
GeneIDi 854073.
KEGGi sce:YOL081W.

Organism-specific databases

CYGDi YOL081w.
SGDi S000005441. IRA2.

Phylogenomic databases

eggNOGi NOG255236.
GeneTreei ENSGT00550000074797.
HOGENOMi HOG000113101.
OMAi FQVLHET.
OrthoDBi EOG7H1JTR.

Enzyme and pathway databases

BioCyci YEAST:G3O-33484-MONOMER.

Miscellaneous databases

NextBioi 975695.

Gene expression databases

Genevestigatori P19158.

Family and domain databases

Gene3Di 1.10.506.10. 1 hit.
InterProi IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view ]
Pfami PF00616. RasGAP. 1 hit.
[Graphical view ]
SMARTi SM00323. RasGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein."
    Tanaka K., Nakafuku M., Tamanoi F., Kaziro Y., Matsumoto K., Toh-e A.
    Mol. Cell. Biol. 10:4303-4313(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequence of a 10.27 kb segment on the left arm of chromosome XV from Saccharomyces cerevisiae includes part of the IRA2 gene and a putative new gene."
    Zumstein E., Griffin H., Schweizer M.
    Yeast 10:1383-1387(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
    Strain: ATCC 96604 / S288c / FY1679.
  5. "A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames."
    Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.
    Yeast 11:975-986(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
    Strain: ATCC 96604 / S288c / FY1679.
  6. "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
    Tzermia M., Katsoulou C., Alexandraki D.
    Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1982-3079.
  7. "The CCS1 gene from Saccharomyces cerevisiae which is involved in mitochondrial functions is identified as IRA2 an attenuator of RAS1 and RAS2 gene products."
    Bussereau F., Dupont C.H., Boy-Marcotte E., Mallet L., Jacquet M.
    Curr. Genet. 21:325-329(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CCS1 AS IRA2.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIRA2_YEAST
AccessioniPrimary (citable) accession number: P19158
Secondary accession number(s): D6W1Y7, O13592, Q08239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: September 5, 2006
Last modified: September 3, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi