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P19158

- IRA2_YEAST

UniProt

P19158 - IRA2_YEAST

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Protein

Inhibitory regulator protein IRA2

Gene

IRA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase activity of Ras proteins.

GO - Molecular functioni

  1. Ras GTPase activator activity Source: SGD

GO - Biological processi

  1. cellular response to cold Source: SGD
  2. cellular response to hydrogen peroxide Source: SGD
  3. cellular response to metal ion Source: SGD
  4. cellular response to oxidative stress Source: SGD
  5. negative regulation of cAMP biosynthetic process Source: SGD
  6. negative regulation of Ras protein signal transduction Source: SGD
  7. positive regulation of Ras GTPase activity Source: SGD
  8. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-33484-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory regulator protein IRA2
Gene namesi
Name:IRA2
Synonyms:CCS1, GLC4
Ordered Locus Names:YOL081W
ORF Names:O0985
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL081w.
SGDiS000005441. IRA2.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30793079Inhibitory regulator protein IRA2PRO_0000056658Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei635 – 6351Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP19158.
PaxDbiP19158.
PeptideAtlasiP19158.

Expressioni

Gene expression databases

GenevestigatoriP19158.

Interactioni

Protein-protein interaction databases

BioGridi34321. 575 interactions.
DIPiDIP-6376N.
IntActiP19158. 2 interactions.
MINTiMINT-597224.

Structurei

3D structure databases

ProteinModelPortaliP19158.
SMRiP19158. Positions 1706-1959.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1701 – 1890190Ras-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi399 – 40911Poly-SerAdd
BLAST
Compositional biasi412 – 4165Poly-Ser
Compositional biasi520 – 5289Poly-Ala
Compositional biasi2469 – 24724Poly-Leu

Sequence similaritiesi

Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG255236.
GeneTreeiENSGT00550000074797.
HOGENOMiHOG000113101.
InParanoidiP19158.
OMAiFQVLHET.
OrthoDBiEOG7H1JTR.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
InterProiIPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19158-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQPTKNKKK EHGTDSKSSR MTRTLVNHIL FERILPILPV ESNLSTYSEV
60 70 80 90 100
EEYSSFISCR SVLINVTVSR DANAMVEGTL ELIESLLQGH EIISDKGSSD
110 120 130 140 150
VIESILIILR LLSDALEYNW QNQESLHYND ISTHVEHDQE QKYRPKLNSI
160 170 180 190 200
LPDYSSTHSN GNKHFFHQSK PQALIPELAS KLLESCAKLK FNTRTLQILQ
210 220 230 240 250
NMISHVHGNI LTTLSSSILP RHKSYLTRHN HPSHCKMIDS TLGHILRFVA
260 270 280 290 300
ASNPSEYFEF IRKSVQVPVT QTHTHSHSHS HSLPSSVYNS IVPHFDLFSF
310 320 330 340 350
IYLSKHNFKK YLELIKNLSV TLRKTIYHCL LLHYSAKAIM FWIMARPAEY
360 370 380 390 400
YELFNLLKDN NNEHSKSLNT LNHTLFEEIH STFNVNSMIT TNQNAHQGSS
410 420 430 440 450
SPSSSSPSSP PSSSSSDNNN QNIIAKSLSR QLSHHQSYIQ QQSERKLHSS
460 470 480 490 500
WTTNSQSSTS LSSSTSNSTT TDFSTHTQPG EYDPSLPDTP TMSNITISAS
510 520 530 540 550
SLLSQTPTPT TQLQQRLNSA AAAAAAAASP SNSTPTGYTA EQQSRASYDA
560 570 580 590 600
HKTGHTGKDY DEHFLSVTRL DNVLELYTHF DDTEVLPHTS VLKFLTTLTM
610 620 630 640 650
FDIDLFNELN ATSFKYIPDC TMHRPKERTS SFNNTAHETG SEKTSGIKHI
660 670 680 690 700
TQGLKKLTSL PSSTKKTVKF VKMLLRNLNG NQAVSDVALL DTMRALLSFF
710 720 730 740 750
TMTSAVFLVD RNLPSVLFAK RLIPIMGTNL SVGQDWNSKI NNSLMVCLKK
760 770 780 790 800
NSTTFVQLQL IFFSSAIQFD HELLLARLSI DTMANNLNMQ KLCLYTEGFR
810 820 830 840 850
IFFDIPSKKE LRKAIAVKIS KFFKTLFSII ADILLQEFPY FDEQITDIVA
860 870 880 890 900
SILDGTIINE YGTKKHFKGS SPSLCSTTRS RSGSTSQSSM TPVSPLGLDT
910 920 930 940 950
DICPMNTLSL VGSSTSRNSD NVNSLNSSPK NLSSDPYLSH LVAPRARHAL
960 970 980 990 1000
GGPSSIIRNK IPTTLTSPPG TEKSSPVQRP QTESISATPM AITNSTPLSS
1010 1020 1030 1040 1050
AAFGIRSPLQ KIRTRRYSDE SLGKFMKSTN NYIQEHLIPK DLNEATLQDA
1060 1070 1080 1090 1100
RRIMINIFSI FKRPNSYFII PHNINSNLQW VSQDFRNIMK PIFVAIVSPD
1110 1120 1130 1140 1150
VDLQNTAQSF MDTLLSNVIT YGESDENISI EGYHLLCSYT VTLFAMGLFD
1160 1170 1180 1190 1200
LKINNEKRQI LLDITVKFMK VRSHLAGIAE ASHHMEYISD SEKLTFPLIM
1210 1220 1230 1240 1250
GTVGRALFVS LYSSQQKIEK TLKIAYTEYL SAINFHERNI DDADKTWVHN
1260 1270 1280 1290 1300
IEFVEAMCHD NYTTSGSIAF QRRTRNNILR FATIPNAILL DSMRMIYKKW
1310 1320 1330 1340 1350
HTYTHSKSLE KQERNDFRNF AGILASLSGI LFINKKILQE MYPYLLDTVS
1360 1370 1380 1390 1400
ELKKNIDSFI SKQCQWLNYP DLLTRENSRD ILSVELHPLS FNLLFNNLRL
1410 1420 1430 1440 1450
KLKELACSDL SIPENESSYV LLEQIIKMLR TILGRDDDNY VMMLFSTEIV
1460 1470 1480 1490 1500
DLIDLLTDEI KKIPAYCPKY LKAIIQMTKM FSALQHSEVN LGVKNHFHVK
1510 1520 1530 1540 1550
NKWLRQITDW FQVSIAREYD FENLSKPLKE MDLVKRDMDI LYIDTAIEAS
1560 1570 1580 1590 1600
TAIAYLTRHT FLEIPPAASD PELSRSRSVI FGFYFNILMK GLEKSSDRDN
1610 1620 1630 1640 1650
YPVFLRHKMS VLNDNVILSL TNLSNTNVDA SLQFTLPMGY SGNRNIRNAF
1660 1670 1680 1690 1700
LEVFINIVTN YRTYTAKTDL GKLEAADKFL RYTIEHPQLS SFGAAVCPAS
1710 1720 1730 1740 1750
DIDAYAAGLI NAFETRNATH IVVAQLIKNE IEKSSRPTDI LRRNSCATRS
1760 1770 1780 1790 1800
LSMLARSKGN EYLIRTLQPL LKKIIQNRDF FEIEKLKPED SDAERQIELF
1810 1820 1830 1840 1850
VKYMNELLES ISNSVSYFPP PLFYICQNIY KVACEKFPDH AIIAAGSFVF
1860 1870 1880 1890 1900
LRFFCPALVS PDSENIIDIS HLSEKRTFIS LAKVIQNIAN GSENFSRWPA
1910 1920 1930 1940 1950
LCSQKDFLKE CSDRIFRFLA ELCRTDRTID IQVRTDPTPI AFDYQFLHSF
1960 1970 1980 1990 2000
VYLYGLEVRR NVLNEAKHDD GDIDGDDFYK TTFLLIDDVL GQLGQPKMEF
2010 2020 2030 2040 2050
SNEIPIYIRE HMDDYPELYE FMNRHAFRNI ETSTAYSPSV HESTSSEGIP
2060 2070 2080 2090 2100
IITLTMSNFS DRHVDIDTVA YKFLQIYARI WTTKHCLIID CTEFDEGGLD
2110 2120 2130 2140 2150
MRKFISLVMG LLPEVAPKNC IGCYYFNVNE TFMDNYGKCL DKDNVYVSSK
2160 2170 2180 2190 2200
IPHYFINSNS DEGLMKSVGI TGQGLKVLQD IRVSLHDITL YDEKRNRFTP
2210 2220 2230 2240 2250
VSLKIGDIYF QVLHETPRQY KIRDMGTLFD VKFNDVYEIS RIFEVHVSSI
2260 2270 2280 2290 2300
TGVAAEFTVT FQDERRLIFS SPKYLEIVKM FYYAQIRLES EYEMDNNSST
2310 2320 2330 2340 2350
SSPNSNNKDK QQKERTKLLC HLLLVSLIGL FDESKKMKNS SYNLIAATEA
2360 2370 2380 2390 2400
SFGLNFGSHF HRSPEVYVPE DTTTFLGVIG KSLAESNPEL TAYMFIYVLE
2410 2420 2430 2440 2450
ALKNNVIPHV YIPHTICGLS YWIPNLYQHV YLADDEEGPE NISHIFRILI
2460 2470 2480 2490 2500
RLSVRETDFK AVYMQYVWLL LLDDGRLTDI IVDEVINHAL ERDSENRDWK
2510 2520 2530 2540 2550
KTISLLTVLP TTEVANNIIQ KILAKIRSFL PSLKLEAMTQ SWSELTILVK
2560 2570 2580 2590 2600
ISIHVFFETS LLVQMYLPEI LFIVSLLIDV GPRELRSSLH QLLMNVCHSL
2610 2620 2630 2640 2650
AINSALPQDH RNNLDEISDI FAHQKVKFMF GFSEDKGRIL QIFSASSFAS
2660 2670 2680 2690 2700
KFNILDFFIN NILLLMEYSS TYEANVWKTR YKKYVLESVF TSNSFLSARS
2710 2720 2730 2740 2750
IMIVGIMGKS YITEGLCKAM LIETMKVIAE PKITDEHLFL AISHIFTYSK
2760 2770 2780 2790 2800
IVEGLDPNLD LMKHLFWFST LFLESRHPII FEGALLFVSN CIRRLYMAQF
2810 2820 2830 2840 2850
ENESETSLIS TLLKGRKFAH TFLSKIENLS GIVWNEDNFT HILIFIINKG
2860 2870 2880 2890 2900
LSNPFIKSTA FDFLKMMFRN SYFEHQINQK SDHYLCYMFL LYFVLNCNQF
2910 2920 2930 2940 2950
EELLGDVDFE GEMVNIENKN TIPKILLEWL SSDNENANIT LYQGAILFKC
2960 2970 2980 2990 3000
SVTDEPSRFR FALIIRHLLT KKPICALRFY SVIRNEIRKI SAFEQNSDCV
3010 3020 3030 3040 3050
PLAFDILNLL VTHSESNSLE KLHEESIERL TKRGLSIVTS SGIFAKNSDM
3060 3070
MIPLDVKPED IYERKRIMTM ILSRMSCSA
Length:3,079
Mass (Da):351,669
Last modified:September 5, 2006 - v2
Checksum:iC14B5396D6FB9B5C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2309 – 23091D → V in AAA34710. (PubMed:2164637)Curated
Sequence conflicti2317 – 23171K → I in AAA34710. (PubMed:2164637)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33779 Genomic DNA. Translation: AAA34710.1.
Z74823 Genomic DNA. Translation: CAA99093.1.
X75449 Genomic DNA. Translation: CAA53202.1.
X83121 Genomic DNA. Translation: CAA58201.1.
Z74822 Genomic DNA. Translation: CAA99092.1.
BK006948 Genomic DNA. Translation: DAA10703.1.
PIRiS66775. RGBYI2.
RefSeqiNP_014560.1. NM_001183335.1.

Genome annotation databases

EnsemblFungiiYOL081W; YOL081W; YOL081W.
GeneIDi854073.
KEGGisce:YOL081W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33779 Genomic DNA. Translation: AAA34710.1 .
Z74823 Genomic DNA. Translation: CAA99093.1 .
X75449 Genomic DNA. Translation: CAA53202.1 .
X83121 Genomic DNA. Translation: CAA58201.1 .
Z74822 Genomic DNA. Translation: CAA99092.1 .
BK006948 Genomic DNA. Translation: DAA10703.1 .
PIRi S66775. RGBYI2.
RefSeqi NP_014560.1. NM_001183335.1.

3D structure databases

ProteinModelPortali P19158.
SMRi P19158. Positions 1706-1959.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34321. 575 interactions.
DIPi DIP-6376N.
IntActi P19158. 2 interactions.
MINTi MINT-597224.

Proteomic databases

MaxQBi P19158.
PaxDbi P19158.
PeptideAtlasi P19158.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL081W ; YOL081W ; YOL081W .
GeneIDi 854073.
KEGGi sce:YOL081W.

Organism-specific databases

CYGDi YOL081w.
SGDi S000005441. IRA2.

Phylogenomic databases

eggNOGi NOG255236.
GeneTreei ENSGT00550000074797.
HOGENOMi HOG000113101.
InParanoidi P19158.
OMAi FQVLHET.
OrthoDBi EOG7H1JTR.

Enzyme and pathway databases

BioCyci YEAST:G3O-33484-MONOMER.

Miscellaneous databases

NextBioi 975695.

Gene expression databases

Genevestigatori P19158.

Family and domain databases

Gene3Di 1.10.506.10. 1 hit.
InterProi IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view ]
Pfami PF00616. RasGAP. 1 hit.
[Graphical view ]
SMARTi SM00323. RasGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein."
    Tanaka K., Nakafuku M., Tamanoi F., Kaziro Y., Matsumoto K., Toh-e A.
    Mol. Cell. Biol. 10:4303-4313(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequence of a 10.27 kb segment on the left arm of chromosome XV from Saccharomyces cerevisiae includes part of the IRA2 gene and a putative new gene."
    Zumstein E., Griffin H., Schweizer M.
    Yeast 10:1383-1387(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
    Strain: ATCC 96604 / S288c / FY1679.
  5. "A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames."
    Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.
    Yeast 11:975-986(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
    Strain: ATCC 96604 / S288c / FY1679.
  6. "Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
    Tzermia M., Katsoulou C., Alexandraki D.
    Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1982-3079.
  7. "The CCS1 gene from Saccharomyces cerevisiae which is involved in mitochondrial functions is identified as IRA2 an attenuator of RAS1 and RAS2 gene products."
    Bussereau F., Dupont C.H., Boy-Marcotte E., Mallet L., Jacquet M.
    Curr. Genet. 21:325-329(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF CCS1 AS IRA2.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIRA2_YEAST
AccessioniPrimary (citable) accession number: P19158
Secondary accession number(s): D6W1Y7, O13592, Q08239
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3