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P19158 (IRA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inhibitory regulator protein IRA2
Gene names
Name:IRA2
Synonyms:CCS1, GLC4
Ordered Locus Names:YOL081W
ORF Names:O0985
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length3079 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase activity of Ras proteins.

Subcellular location

Cytoplasm Ref.8.

Sequence similarities

Contains 1 Ras-GAP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 30793079Inhibitory regulator protein IRA2
PRO_0000056658

Regions

Domain1701 – 1890190Ras-GAP
Compositional bias399 – 40911Poly-Ser
Compositional bias412 – 4165Poly-Ser
Compositional bias520 – 5289Poly-Ala
Compositional bias2469 – 24724Poly-Leu

Amino acid modifications

Modified residue6351Phosphothreonine Ref.11

Experimental info

Sequence conflict23091D → V in AAA34710. Ref.1
Sequence conflict23171K → I in AAA34710. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19158 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: C14B5396D6FB9B5C

FASTA3,079351,669
        10         20         30         40         50         60 
MSQPTKNKKK EHGTDSKSSR MTRTLVNHIL FERILPILPV ESNLSTYSEV EEYSSFISCR 

        70         80         90        100        110        120 
SVLINVTVSR DANAMVEGTL ELIESLLQGH EIISDKGSSD VIESILIILR LLSDALEYNW 

       130        140        150        160        170        180 
QNQESLHYND ISTHVEHDQE QKYRPKLNSI LPDYSSTHSN GNKHFFHQSK PQALIPELAS 

       190        200        210        220        230        240 
KLLESCAKLK FNTRTLQILQ NMISHVHGNI LTTLSSSILP RHKSYLTRHN HPSHCKMIDS 

       250        260        270        280        290        300 
TLGHILRFVA ASNPSEYFEF IRKSVQVPVT QTHTHSHSHS HSLPSSVYNS IVPHFDLFSF 

       310        320        330        340        350        360 
IYLSKHNFKK YLELIKNLSV TLRKTIYHCL LLHYSAKAIM FWIMARPAEY YELFNLLKDN 

       370        380        390        400        410        420 
NNEHSKSLNT LNHTLFEEIH STFNVNSMIT TNQNAHQGSS SPSSSSPSSP PSSSSSDNNN 

       430        440        450        460        470        480 
QNIIAKSLSR QLSHHQSYIQ QQSERKLHSS WTTNSQSSTS LSSSTSNSTT TDFSTHTQPG 

       490        500        510        520        530        540 
EYDPSLPDTP TMSNITISAS SLLSQTPTPT TQLQQRLNSA AAAAAAAASP SNSTPTGYTA 

       550        560        570        580        590        600 
EQQSRASYDA HKTGHTGKDY DEHFLSVTRL DNVLELYTHF DDTEVLPHTS VLKFLTTLTM 

       610        620        630        640        650        660 
FDIDLFNELN ATSFKYIPDC TMHRPKERTS SFNNTAHETG SEKTSGIKHI TQGLKKLTSL 

       670        680        690        700        710        720 
PSSTKKTVKF VKMLLRNLNG NQAVSDVALL DTMRALLSFF TMTSAVFLVD RNLPSVLFAK 

       730        740        750        760        770        780 
RLIPIMGTNL SVGQDWNSKI NNSLMVCLKK NSTTFVQLQL IFFSSAIQFD HELLLARLSI 

       790        800        810        820        830        840 
DTMANNLNMQ KLCLYTEGFR IFFDIPSKKE LRKAIAVKIS KFFKTLFSII ADILLQEFPY 

       850        860        870        880        890        900 
FDEQITDIVA SILDGTIINE YGTKKHFKGS SPSLCSTTRS RSGSTSQSSM TPVSPLGLDT 

       910        920        930        940        950        960 
DICPMNTLSL VGSSTSRNSD NVNSLNSSPK NLSSDPYLSH LVAPRARHAL GGPSSIIRNK 

       970        980        990       1000       1010       1020 
IPTTLTSPPG TEKSSPVQRP QTESISATPM AITNSTPLSS AAFGIRSPLQ KIRTRRYSDE 

      1030       1040       1050       1060       1070       1080 
SLGKFMKSTN NYIQEHLIPK DLNEATLQDA RRIMINIFSI FKRPNSYFII PHNINSNLQW 

      1090       1100       1110       1120       1130       1140 
VSQDFRNIMK PIFVAIVSPD VDLQNTAQSF MDTLLSNVIT YGESDENISI EGYHLLCSYT 

      1150       1160       1170       1180       1190       1200 
VTLFAMGLFD LKINNEKRQI LLDITVKFMK VRSHLAGIAE ASHHMEYISD SEKLTFPLIM 

      1210       1220       1230       1240       1250       1260 
GTVGRALFVS LYSSQQKIEK TLKIAYTEYL SAINFHERNI DDADKTWVHN IEFVEAMCHD 

      1270       1280       1290       1300       1310       1320 
NYTTSGSIAF QRRTRNNILR FATIPNAILL DSMRMIYKKW HTYTHSKSLE KQERNDFRNF 

      1330       1340       1350       1360       1370       1380 
AGILASLSGI LFINKKILQE MYPYLLDTVS ELKKNIDSFI SKQCQWLNYP DLLTRENSRD 

      1390       1400       1410       1420       1430       1440 
ILSVELHPLS FNLLFNNLRL KLKELACSDL SIPENESSYV LLEQIIKMLR TILGRDDDNY 

      1450       1460       1470       1480       1490       1500 
VMMLFSTEIV DLIDLLTDEI KKIPAYCPKY LKAIIQMTKM FSALQHSEVN LGVKNHFHVK 

      1510       1520       1530       1540       1550       1560 
NKWLRQITDW FQVSIAREYD FENLSKPLKE MDLVKRDMDI LYIDTAIEAS TAIAYLTRHT 

      1570       1580       1590       1600       1610       1620 
FLEIPPAASD PELSRSRSVI FGFYFNILMK GLEKSSDRDN YPVFLRHKMS VLNDNVILSL 

      1630       1640       1650       1660       1670       1680 
TNLSNTNVDA SLQFTLPMGY SGNRNIRNAF LEVFINIVTN YRTYTAKTDL GKLEAADKFL 

      1690       1700       1710       1720       1730       1740 
RYTIEHPQLS SFGAAVCPAS DIDAYAAGLI NAFETRNATH IVVAQLIKNE IEKSSRPTDI 

      1750       1760       1770       1780       1790       1800 
LRRNSCATRS LSMLARSKGN EYLIRTLQPL LKKIIQNRDF FEIEKLKPED SDAERQIELF 

      1810       1820       1830       1840       1850       1860 
VKYMNELLES ISNSVSYFPP PLFYICQNIY KVACEKFPDH AIIAAGSFVF LRFFCPALVS 

      1870       1880       1890       1900       1910       1920 
PDSENIIDIS HLSEKRTFIS LAKVIQNIAN GSENFSRWPA LCSQKDFLKE CSDRIFRFLA 

      1930       1940       1950       1960       1970       1980 
ELCRTDRTID IQVRTDPTPI AFDYQFLHSF VYLYGLEVRR NVLNEAKHDD GDIDGDDFYK 

      1990       2000       2010       2020       2030       2040 
TTFLLIDDVL GQLGQPKMEF SNEIPIYIRE HMDDYPELYE FMNRHAFRNI ETSTAYSPSV 

      2050       2060       2070       2080       2090       2100 
HESTSSEGIP IITLTMSNFS DRHVDIDTVA YKFLQIYARI WTTKHCLIID CTEFDEGGLD 

      2110       2120       2130       2140       2150       2160 
MRKFISLVMG LLPEVAPKNC IGCYYFNVNE TFMDNYGKCL DKDNVYVSSK IPHYFINSNS 

      2170       2180       2190       2200       2210       2220 
DEGLMKSVGI TGQGLKVLQD IRVSLHDITL YDEKRNRFTP VSLKIGDIYF QVLHETPRQY 

      2230       2240       2250       2260       2270       2280 
KIRDMGTLFD VKFNDVYEIS RIFEVHVSSI TGVAAEFTVT FQDERRLIFS SPKYLEIVKM 

      2290       2300       2310       2320       2330       2340 
FYYAQIRLES EYEMDNNSST SSPNSNNKDK QQKERTKLLC HLLLVSLIGL FDESKKMKNS 

      2350       2360       2370       2380       2390       2400 
SYNLIAATEA SFGLNFGSHF HRSPEVYVPE DTTTFLGVIG KSLAESNPEL TAYMFIYVLE 

      2410       2420       2430       2440       2450       2460 
ALKNNVIPHV YIPHTICGLS YWIPNLYQHV YLADDEEGPE NISHIFRILI RLSVRETDFK 

      2470       2480       2490       2500       2510       2520 
AVYMQYVWLL LLDDGRLTDI IVDEVINHAL ERDSENRDWK KTISLLTVLP TTEVANNIIQ 

      2530       2540       2550       2560       2570       2580 
KILAKIRSFL PSLKLEAMTQ SWSELTILVK ISIHVFFETS LLVQMYLPEI LFIVSLLIDV 

      2590       2600       2610       2620       2630       2640 
GPRELRSSLH QLLMNVCHSL AINSALPQDH RNNLDEISDI FAHQKVKFMF GFSEDKGRIL 

      2650       2660       2670       2680       2690       2700 
QIFSASSFAS KFNILDFFIN NILLLMEYSS TYEANVWKTR YKKYVLESVF TSNSFLSARS 

      2710       2720       2730       2740       2750       2760 
IMIVGIMGKS YITEGLCKAM LIETMKVIAE PKITDEHLFL AISHIFTYSK IVEGLDPNLD 

      2770       2780       2790       2800       2810       2820 
LMKHLFWFST LFLESRHPII FEGALLFVSN CIRRLYMAQF ENESETSLIS TLLKGRKFAH 

      2830       2840       2850       2860       2870       2880 
TFLSKIENLS GIVWNEDNFT HILIFIINKG LSNPFIKSTA FDFLKMMFRN SYFEHQINQK 

      2890       2900       2910       2920       2930       2940 
SDHYLCYMFL LYFVLNCNQF EELLGDVDFE GEMVNIENKN TIPKILLEWL SSDNENANIT 

      2950       2960       2970       2980       2990       3000 
LYQGAILFKC SVTDEPSRFR FALIIRHLLT KKPICALRFY SVIRNEIRKI SAFEQNSDCV 

      3010       3020       3030       3040       3050       3060 
PLAFDILNLL VTHSESNSLE KLHEESIERL TKRGLSIVTS SGIFAKNSDM MIPLDVKPED 

      3070 
IYERKRIMTM ILSRMSCSA 

« Hide

References

« Hide 'large scale' references
[1]"IRA2, a second gene of Saccharomyces cerevisiae that encodes a protein with a domain homologous to mammalian ras GTPase-activating protein."
Tanaka K., Nakafuku M., Tamanoi F., Kaziro Y., Matsumoto K., Toh-e A.
Mol. Cell. Biol. 10:4303-4313(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Sequence of a 10.27 kb segment on the left arm of chromosome XV from Saccharomyces cerevisiae includes part of the IRA2 gene and a putative new gene."
Zumstein E., Griffin H., Schweizer M.
Yeast 10:1383-1387(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
Strain: ATCC 96604 / S288c / FY1679.
[5]"A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames."
Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.
Yeast 11:975-986(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2423.
Strain: ATCC 96604 / S288c / FY1679.
[6]"Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags."
Tzermia M., Katsoulou C., Alexandraki D.
Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1982-3079.
[7]"The CCS1 gene from Saccharomyces cerevisiae which is involved in mitochondrial functions is identified as IRA2 an attenuator of RAS1 and RAS2 gene products."
Bussereau F., Dupont C.H., Boy-Marcotte E., Mallet L., Jacquet M.
Curr. Genet. 21:325-329(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF CCS1 AS IRA2.
[8]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33779 Genomic DNA. Translation: AAA34710.1.
Z74823 Genomic DNA. Translation: CAA99093.1.
X75449 Genomic DNA. Translation: CAA53202.1.
X83121 Genomic DNA. Translation: CAA58201.1.
Z74822 Genomic DNA. Translation: CAA99092.1.
BK006948 Genomic DNA. Translation: DAA10703.1.
PIRRGBYI2. S66775.
RefSeqNP_014560.1. NM_001183335.1.

3D structure databases

ProteinModelPortalP19158.
SMRP19158. Positions 1673-1997.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34321. 570 interactions.
DIPDIP-6376N.
IntActP19158. 2 interactions.
MINTMINT-597224.

Proteomic databases

PaxDbP19158.
PeptideAtlasP19158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL081W; YOL081W; YOL081W.
GeneID854073.
KEGGsce:YOL081W.

Organism-specific databases

CYGDYOL081w.
SGDS000005441. IRA2.

Phylogenomic databases

eggNOGNOG255236.
GeneTreeENSGT00550000074797.
HOGENOMHOG000113101.
OMAFQVLHET.
OrthoDBEOG7H1JTR.

Enzyme and pathway databases

BioCycYEAST:G3O-33484-MONOMER.

Gene expression databases

GenevestigatorP19158.

Family and domain databases

Gene3D1.10.506.10. 1 hit.
InterProIPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamPF00616. RasGAP. 1 hit.
[Graphical view]
SMARTSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
PROSITEPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975695.

Entry information

Entry nameIRA2_YEAST
AccessionPrimary (citable) accession number: P19158
Secondary accession number(s): D6W1Y7, O13592, Q08239
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families