Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium-transporting ATPase alpha chain 1

Gene

ATP4A

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3864-aspartylphosphate intermediate1 Publication1
Metal bindingi727MagnesiumBy similarity1
Metal bindingi731MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi3.6.3.10. 6170.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 1 (EC:3.6.3.10)
Alternative name(s):
Gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:ATP4A
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 97CytoplasmicSequence analysisAdd BLAST96
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 141LumenalSequence analysisAdd BLAST23
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Topological domaini163 – 298CytoplasmicSequence analysisAdd BLAST136
Transmembranei299 – 318HelicalSequence analysisAdd BLAST20
Topological domaini319 – 330LumenalSequence analysisAdd BLAST12
Transmembranei331 – 348HelicalSequence analysisAdd BLAST18
Topological domaini349 – 782CytoplasmicSequence analysisAdd BLAST434
Transmembranei783 – 802HelicalSequence analysisAdd BLAST20
Topological domaini803 – 812LumenalSequence analysis10
Transmembranei813 – 833HelicalSequence analysisAdd BLAST21
Topological domaini834 – 853CytoplasmicSequence analysisAdd BLAST20
Transmembranei854 – 876HelicalSequence analysisAdd BLAST23
Topological domaini877 – 928LumenalSequence analysisAdd BLAST52
Transmembranei929 – 948HelicalSequence analysisAdd BLAST20
Topological domaini949 – 962CytoplasmicSequence analysisAdd BLAST14
Transmembranei963 – 981HelicalSequence analysisAdd BLAST19
Topological domaini982 – 996LumenalSequence analysisAdd BLAST15
Transmembranei997 – 1017HelicalSequence analysisAdd BLAST21
Topological domaini1018 – 1034CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3919.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000462552 – 1034Potassium-transporting ATPase alpha chain 1Add BLAST1033

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7Phosphotyrosine1 Publication1
Modified residuei10Phosphotyrosine1 Publication1
Modified residuei27Phosphoserine; by PKA and PKC1 Publication1
Modified residuei57PhosphoserineBy similarity1
Modified residuei76PhosphoserineBy similarity1
Modified residuei238PhosphoserineBy similarity1
Modified residuei285PhosphoserineBy similarity1
Modified residuei462PhosphoserineBy similarity1
Modified residuei600PhosphoserineBy similarity1
Modified residuei839PhosphoserineBy similarity1
Modified residuei953Phosphoserine; by PKABy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19156.
PeptideAtlasiP19156.
PRIDEiP19156.

PTM databases

iPTMnetiP19156.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Protein-protein interaction databases

IntActiP19156. 1 interactor.
STRINGi9823.ENSSSCP00000003126.

Chemistry databases

BindingDBiP19156.

Structurei

Secondary structure

11034
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 14Combined sources10
Beta strandi18 – 23Combined sources6
Helixi24 – 29Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWCNMR-A1-34[»]
1IWFNMR-A1-34[»]
2XZBelectron microscopy7.00A1-1034[»]
2YN9electron microscopy8.00A1-1034[»]
3IXZelectron microscopy6.50A1-1034[»]
4UX1electron microscopy8.00A1-1034[»]
4UX2electron microscopy7.00A1-1034[»]
ProteinModelPortaliP19156.
SMRiP19156.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19156.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP19156.
KOiK01542.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19156-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKAENYELY QVELGPGPSG DMAAKMSKKK AGRGGGKRKE KLENMKKEME
60 70 80 90 100
INDHQLSVAE LEQKYQTSAT KGLSASLAAE LLLRDGPNAL RPPRGTPEYV
110 120 130 140 150
KFARQLAGGL QCLMWVAAAI CLIAFAIQAS EGDLTTDDNL YLALALIAVV
160 170 180 190 200
VVTGCFGYYQ EFKSTNIIAS FKNLVPQQAT VIRDGDKFQI NADQLVVGDL
210 220 230 240 250
VEMKGGDRVP ADIRILQAQG RKVDNSSLTG ESEPQTRSPE CTHESPLETR
260 270 280 290 300
NIAFFSTMCL EGTAQGLVVN TGDRTIIGRI ASLASGVENE KTPIAIEIEH
310 320 330 340 350
FVDIIAGLAI LFGATFFIVA MCIGYTFLRA MVFFMAIVVA YVPEGLLATV
360 370 380 390 400
TVCLSLTAKR LASKNCVVKN LEAVETLGST SVICSDKTGT LTQNRMTVSH
410 420 430 440 450
LWFDNHIHSA DTTEDQSGQT FDQSSETWRA LCRVLTLCNR AAFKSGQDAV
460 470 480 490 500
PVPKRIVIGD ASETALLKFS ELTLGNAMGY RERFPKVCEI PFNSTNKFQL
510 520 530 540 550
SIHTLEDPRD PRHVLVMKGA PERVLERCSS ILIKGQELPL DEQWREAFQT
560 570 580 590 600
AYLSLGGLGE RVLGFCQLYL SEKDYPPGYA FDVEAMNFPT SGLSFAGLVS
610 620 630 640 650
MIDPPRATVP DAVLKCRTAG IRVIMVTGDH PITAKAIAAS VGIISEGSET
660 670 680 690 700
VEDIAARLRV PVDQVNRKDA RACVINGMQL KDMDPSELVE ALRTHPEMVF
710 720 730 740 750
ARTSPQQKLV IVESCQRLGA IVAVTGDGVN DSPALKKADI GVAMGIAGSD
760 770 780 790 800
AAKNAADMIL LDDNFASIVT GVEQGRLIFD NLKKSIAYTL TKNIPELTPY
810 820 830 840 850
LIYITVSVPL PLGCITILFI ELCTDIFPSV SLAYEKAESD IMHLRPRNPK
860 870 880 890 900
RDRLVNEPLA AYSYFQIGAI QSFAGFTDYF TAMAQEGWFP LLCVGLRPQW
910 920 930 940 950
ENHHLQDLQD SYGQEWTFGQ RLYQQYTCYT VFFISIEMCQ IADVLIRKTR
960 970 980 990 1000
RLSAFQQGFF RNRILVIAIV FQVCIGCFLC YCPGMPNIFN FMPIRFQWWL
1010 1020 1030
VPMPFGLLIF VYDEIRKLGV RCCPGSWWDQ ELYY
Length:1,034
Mass (Da):114,287
Last modified:January 23, 2007 - v3
Checksum:i97077AC0ADEA8DDE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2G → Y AA sequence (PubMed:3017315).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22724 mRNA. Translation: AAA31003.1.
PIRiA31671.
RefSeqiNP_999456.1. NM_214291.1.
UniGeneiSsc.80980.

Genome annotation databases

GeneIDi397552.
KEGGissc:397552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22724 mRNA. Translation: AAA31003.1.
PIRiA31671.
RefSeqiNP_999456.1. NM_214291.1.
UniGeneiSsc.80980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IWCNMR-A1-34[»]
1IWFNMR-A1-34[»]
2XZBelectron microscopy7.00A1-1034[»]
2YN9electron microscopy8.00A1-1034[»]
3IXZelectron microscopy6.50A1-1034[»]
4UX1electron microscopy8.00A1-1034[»]
4UX2electron microscopy7.00A1-1034[»]
ProteinModelPortaliP19156.
SMRiP19156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19156. 1 interactor.
STRINGi9823.ENSSSCP00000003126.

Chemistry databases

BindingDBiP19156.
ChEMBLiCHEMBL3919.

PTM databases

iPTMnetiP19156.

Proteomic databases

PaxDbiP19156.
PeptideAtlasiP19156.
PRIDEiP19156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397552.
KEGGissc:397552.

Organism-specific databases

CTDi495.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP19156.
KOiK01542.

Enzyme and pathway databases

BRENDAi3.6.3.10. 6170.

Miscellaneous databases

EvolutionaryTraceiP19156.
PROiP19156.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATP4A_PIG
AccessioniPrimary (citable) accession number: P19156
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.