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P19147

- PPB_SERMA

UniProt

P19147 - PPB_SERMA

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Protein

Alkaline phosphatase

Gene

phoA

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg(2+) ion.
  • Zn2+Note: Binds 2 Zn(2+) ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771MagnesiumBy similarity
Metal bindingi77 – 771Zinc 2By similarity
Active sitei128 – 1281Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi179 – 1791MagnesiumBy similarity
Metal bindingi181 – 1811MagnesiumBy similarity
Metal bindingi346 – 3461MagnesiumBy similarity
Metal bindingi353 – 3531Zinc 1By similarity
Metal bindingi357 – 3571Zinc 1By similarity
Metal bindingi395 – 3951Zinc 2By similarity
Metal bindingi396 – 3961Zinc 2By similarity
Metal bindingi438 – 4381Zinc 1By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 475452Alkaline phosphatasePRO_0000024015Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi194 ↔ 204By similarity
Disulfide bondi312 ↔ 362By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP19147.
SMRiP19147. Positions 33-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19147-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQPAVSLIAG AVLSALLCSS AIAAETSANA DGLTDRAARG NLVEPGGARR
60 70 80 90 100
LAGDQTTALK ASLSDKTAKN VILLIGDGMG DSEITAARNY AEGAGGYFKG
110 120 130 140 150
IDALPLTGQY THYSLDRKTH KPDYVTDSAA SATAWATGVK TYNGALGVDV
160 170 180 190 200
NGKDQPTLLE IAKAAGKATG NVSTAELQDA TPAALVSHVI SRKCYGPEET
210 220 230 240 250
SEKCAANALE NGGRGSITEQ LLKTRADVTL GGGAKSFNQL AKSGEWQGKS
260 270 280 290 300
LKDQAAAQGY QWVSNADELQ AVTLANQQKP LLGLFADGNM PVRWLGPKAS
310 320 330 340 350
YHGNLDKPAV TCENNPARTA ATPTLAAMTE KAIALLKDNP NGFFLQVEGA
360 370 380 390 400
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FARADGNTLV IVTADHAHAS
410 420 430 440 450
QIVSADAKAP GLTQKLTTKD GAPMTLSYGN SEEESQGHTG TQLRVAAYGP
460 470
HAANVVGLTD QTDLFFTMRD AMGIK
Length:475
Mass (Da):49,265
Last modified:November 1, 1990 - v1
Checksum:i1B8B9841CBDE6653
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33965 Genomic DNA. Translation: AAA26563.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33965 Genomic DNA. Translation: AAA26563.1 .

3D structure databases

ProteinModelPortali P19147.
SMRi P19147. Positions 33-475.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein."
    Dubose R.F., Hartl D.L.
    Mol. Biol. Evol. 7:547-577(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPPB_SERMA
AccessioniPrimary (citable) accession number: P19147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3