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P19147

- PPB_SERMA

UniProt

P19147 - PPB_SERMA

Protein

Alkaline phosphatase

Gene

phoA

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.
    Binds 2 zinc ions.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771MagnesiumBy similarity
    Metal bindingi77 – 771Zinc 2By similarity
    Active sitei128 – 1281Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi179 – 1791MagnesiumBy similarity
    Metal bindingi181 – 1811MagnesiumBy similarity
    Metal bindingi346 – 3461MagnesiumBy similarity
    Metal bindingi353 – 3531Zinc 1By similarity
    Metal bindingi357 – 3571Zinc 1By similarity
    Metal bindingi395 – 3951Zinc 2By similarity
    Metal bindingi396 – 3961Zinc 2By similarity
    Metal bindingi438 – 4381Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase (EC:3.1.3.1)
    Short name:
    APase
    Gene namesi
    Name:phoA
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 475452Alkaline phosphatasePRO_0000024015Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi194 ↔ 204By similarity
    Disulfide bondi312 ↔ 362By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP19147.
    SMRiP19147. Positions 33-475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 2 hits.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19147-1 [UniParc]FASTAAdd to Basket

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    MQPAVSLIAG AVLSALLCSS AIAAETSANA DGLTDRAARG NLVEPGGARR    50
    LAGDQTTALK ASLSDKTAKN VILLIGDGMG DSEITAARNY AEGAGGYFKG 100
    IDALPLTGQY THYSLDRKTH KPDYVTDSAA SATAWATGVK TYNGALGVDV 150
    NGKDQPTLLE IAKAAGKATG NVSTAELQDA TPAALVSHVI SRKCYGPEET 200
    SEKCAANALE NGGRGSITEQ LLKTRADVTL GGGAKSFNQL AKSGEWQGKS 250
    LKDQAAAQGY QWVSNADELQ AVTLANQQKP LLGLFADGNM PVRWLGPKAS 300
    YHGNLDKPAV TCENNPARTA ATPTLAAMTE KAIALLKDNP NGFFLQVEGA 350
    SIDKQDHAAN PCGQIGETVD LDEAVQKALA FARADGNTLV IVTADHAHAS 400
    QIVSADAKAP GLTQKLTTKD GAPMTLSYGN SEEESQGHTG TQLRVAAYGP 450
    HAANVVGLTD QTDLFFTMRD AMGIK 475
    Length:475
    Mass (Da):49,265
    Last modified:November 1, 1990 - v1
    Checksum:i1B8B9841CBDE6653
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33965 Genomic DNA. Translation: AAA26563.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33965 Genomic DNA. Translation: AAA26563.1 .

    3D structure databases

    ProteinModelPortali P19147.
    SMRi P19147. Positions 33-475.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 2 hits.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein."
      Dubose R.F., Hartl D.L.
      Mol. Biol. Evol. 7:547-577(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPPB_SERMA
    AccessioniPrimary (citable) accession number: P19147
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3