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P19147

- PPB_SERMA

UniProt

P19147 - PPB_SERMA

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Protein
Alkaline phosphatase
Gene
phoA
Organism
Serratia marcescens
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 magnesium ion.
Binds 2 zinc ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Magnesium By similarity
Metal bindingi77 – 771Zinc 2 By similarity
Active sitei128 – 1281Phosphoserine intermediate By similarity
Metal bindingi179 – 1791Magnesium By similarity
Metal bindingi181 – 1811Magnesium By similarity
Metal bindingi346 – 3461Magnesium By similarity
Metal bindingi353 – 3531Zinc 1 By similarity
Metal bindingi357 – 3571Zinc 1 By similarity
Metal bindingi395 – 3951Zinc 2 By similarity
Metal bindingi396 – 3961Zinc 2 By similarity
Metal bindingi438 – 4381Zinc 1 By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 475452Alkaline phosphatase
PRO_0000024015Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi194 ↔ 204 By similarity
Disulfide bondi312 ↔ 362 By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP19147.
SMRiP19147. Positions 33-475.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19147-1 [UniParc]FASTAAdd to Basket

« Hide

MQPAVSLIAG AVLSALLCSS AIAAETSANA DGLTDRAARG NLVEPGGARR    50
LAGDQTTALK ASLSDKTAKN VILLIGDGMG DSEITAARNY AEGAGGYFKG 100
IDALPLTGQY THYSLDRKTH KPDYVTDSAA SATAWATGVK TYNGALGVDV 150
NGKDQPTLLE IAKAAGKATG NVSTAELQDA TPAALVSHVI SRKCYGPEET 200
SEKCAANALE NGGRGSITEQ LLKTRADVTL GGGAKSFNQL AKSGEWQGKS 250
LKDQAAAQGY QWVSNADELQ AVTLANQQKP LLGLFADGNM PVRWLGPKAS 300
YHGNLDKPAV TCENNPARTA ATPTLAAMTE KAIALLKDNP NGFFLQVEGA 350
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FARADGNTLV IVTADHAHAS 400
QIVSADAKAP GLTQKLTTKD GAPMTLSYGN SEEESQGHTG TQLRVAAYGP 450
HAANVVGLTD QTDLFFTMRD AMGIK 475
Length:475
Mass (Da):49,265
Last modified:November 1, 1990 - v1
Checksum:i1B8B9841CBDE6653
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33965 Genomic DNA. Translation: AAA26563.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33965 Genomic DNA. Translation: AAA26563.1 .

3D structure databases

ProteinModelPortali P19147.
SMRi P19147. Positions 33-475.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein."
    Dubose R.F., Hartl D.L.
    Mol. Biol. Evol. 7:547-577(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPPB_SERMA
AccessioniPrimary (citable) accession number: P19147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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