P19147 (PPB_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline phosphatase
Short name=APase
EC=3.1.3.1

Gene names

Name:

phoA

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion. Binds 2 zinc ions.
Subunit structure	Homodimer.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the alkaline phosphatase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond Phosphoprotein
Gene Ontology (GO)
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 475	452	Alkaline phosphatase		PRO_0000024015
Sites
	Active site	128	1	Phosphoserine intermediate By similarity
	Metal binding	77	1	Magnesium By similarity
	Metal binding	77	1	Zinc 2 By similarity
	Metal binding	179	1	Magnesium By similarity
	Metal binding	181	1	Magnesium By similarity
	Metal binding	346	1	Magnesium By similarity
	Metal binding	353	1	Zinc 1 By similarity
	Metal binding	357	1	Zinc 1 By similarity
	Metal binding	395	1	Zinc 2 By similarity
	Metal binding	396	1	Zinc 2 By similarity
	Metal binding	438	1	Zinc 1 By similarity
Amino acid modifications
	Disulfide bond	194 ↔ 204		By similarity
	Disulfide bond	312 ↔ 362		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19147 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 1B8B9841CBDE6653	FASTA	475	49,265
	10 20 30 40 50 60 MQPAVSLIAG AVLSALLCSS AIAAETSANA DGLTDRAARG NLVEPGGARR LAGDQTTALK 70 80 90 100 110 120 ASLSDKTAKN VILLIGDGMG DSEITAARNY AEGAGGYFKG IDALPLTGQY THYSLDRKTH 130 140 150 160 170 180 KPDYVTDSAA SATAWATGVK TYNGALGVDV NGKDQPTLLE IAKAAGKATG NVSTAELQDA 190 200 210 220 230 240 TPAALVSHVI SRKCYGPEET SEKCAANALE NGGRGSITEQ LLKTRADVTL GGGAKSFNQL 250 260 270 280 290 300 AKSGEWQGKS LKDQAAAQGY QWVSNADELQ AVTLANQQKP LLGLFADGNM PVRWLGPKAS 310 320 330 340 350 360 YHGNLDKPAV TCENNPARTA ATPTLAAMTE KAIALLKDNP NGFFLQVEGA SIDKQDHAAN 370 380 390 400 410 420 PCGQIGETVD LDEAVQKALA FARADGNTLV IVTADHAHAS QIVSADAKAP GLTQKLTTKD 430 440 450 460 470 GAPMTLSYGN SEEESQGHTG TQLRVAAYGP HAANVVGLTD QTDLFFTMRD AMGIK

References

[1]	"The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein." Dubose R.F., Hartl D.L. Mol. Biol. Evol. 7:547-577(1990) [PubMed: 2129542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33965 Genomic DNA. Translation: AAA26563.1.
3D structure databases
ProteinModelPortal	P19147.
SMR	P19147. Positions 33-475.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. IPR017850. Alkaline_phosphatase_core. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00245. Alk_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00113. ALKPHPHTASE.
SMART	SM00098. alkPPc. 1 hit. [Graphical view]
SUPFAM	SSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITE	PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPB_SERMA

Accession

Primary (citable) accession number: P19147

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 31, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program