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Reviewed, UniProtKB/Swiss-Prot P19147 (PPB_SERMA)

Last modified June 16, 2009. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alkaline phosphatase
      Short name=APase
    EC=3.1.3.1
Gene names
Name: phoA
OrganismSerratia marcescens
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

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Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion.

Binds 2 zinc ions.

Subunit structure

Homodimer.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the alkaline phosphatase family.

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Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
Phosphoprotein
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalkaline phosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 475452Alkaline phosphatase
PRO_0000024015

Sites

Active site1281Phosphoserine intermediate By similarity
Metal binding771Magnesium By similarity
Metal binding771Zinc 2 By similarity
Metal binding1791Magnesium By similarity
Metal binding1811Magnesium By similarity
Metal binding3461Magnesium By similarity
Metal binding3531Zinc 1 By similarity
Metal binding3571Zinc 1 By similarity
Metal binding3951Zinc 2 By similarity
Metal binding3961Zinc 2 By similarity
Metal binding4381Zinc 1 By similarity

Amino acid modifications

Disulfide bond194 ↔ 204 By similarity
Disulfide bond312 ↔ 362 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P19147-1 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 1B8B9841CBDE6653

FASTA47549,265
        10         20         30         40         50         60 
MQPAVSLIAG AVLSALLCSS AIAAETSANA DGLTDRAARG NLVEPGGARR LAGDQTTALK 

        70         80         90        100        110        120 
ASLSDKTAKN VILLIGDGMG DSEITAARNY AEGAGGYFKG IDALPLTGQY THYSLDRKTH 

       130        140        150        160        170        180 
KPDYVTDSAA SATAWATGVK TYNGALGVDV NGKDQPTLLE IAKAAGKATG NVSTAELQDA 

       190        200        210        220        230        240 
TPAALVSHVI SRKCYGPEET SEKCAANALE NGGRGSITEQ LLKTRADVTL GGGAKSFNQL 

       250        260        270        280        290        300 
AKSGEWQGKS LKDQAAAQGY QWVSNADELQ AVTLANQQKP LLGLFADGNM PVRWLGPKAS 

       310        320        330        340        350        360 
YHGNLDKPAV TCENNPARTA ATPTLAAMTE KAIALLKDNP NGFFLQVEGA SIDKQDHAAN 

       370        380        390        400        410        420 
PCGQIGETVD LDEAVQKALA FARADGNTLV IVTADHAHAS QIVSADAKAP GLTQKLTTKD 

       430        440        450        460        470 
GAPMTLSYGN SEEESQGHTG TQLRVAAYGP HAANVVGLTD QTDLFFTMRD AMGIK

« Hide

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References

[1]"The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein."
Dubose R.F., Hartl D.L.
Mol. Biol. Evol. 7:547-577(1990) [PubMed: 2129542] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

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Cross-references

Sequence databases

M33965 Genomic DNA. Translation: AAA26563.1.

3D structure databases

HSSPHSSP built from PDB template 1AJA based on UniProtKB P00634.
SMRP19147. Positions 33-475.
ModBaseSearch...

Enzyme and pathway databases

BRENDA3.1.3.1. 457.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePPB_SERMA
AccessionPrimary (citable) accession number: P19147
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents