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Protein

Alkaline phosphatase

Gene

phoA

Organism
Serratia marcescens
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77MagnesiumBy similarity1
Metal bindingi77Zinc 2By similarity1
Active sitei128Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi179MagnesiumBy similarity1
Metal bindingi181MagnesiumBy similarity1
Metal bindingi346MagnesiumBy similarity1
Metal bindingi353Zinc 1By similarity1
Metal bindingi357Zinc 1By similarity1
Metal bindingi395Zinc 2By similarity1
Metal bindingi396Zinc 2By similarity1
Metal bindingi438Zinc 1By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000002401524 – 475Alkaline phosphataseAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi194 ↔ 204By similarity
Disulfide bondi312 ↔ 362By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP19147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPAVSLIAG AVLSALLCSS AIAAETSANA DGLTDRAARG NLVEPGGARR
60 70 80 90 100
LAGDQTTALK ASLSDKTAKN VILLIGDGMG DSEITAARNY AEGAGGYFKG
110 120 130 140 150
IDALPLTGQY THYSLDRKTH KPDYVTDSAA SATAWATGVK TYNGALGVDV
160 170 180 190 200
NGKDQPTLLE IAKAAGKATG NVSTAELQDA TPAALVSHVI SRKCYGPEET
210 220 230 240 250
SEKCAANALE NGGRGSITEQ LLKTRADVTL GGGAKSFNQL AKSGEWQGKS
260 270 280 290 300
LKDQAAAQGY QWVSNADELQ AVTLANQQKP LLGLFADGNM PVRWLGPKAS
310 320 330 340 350
YHGNLDKPAV TCENNPARTA ATPTLAAMTE KAIALLKDNP NGFFLQVEGA
360 370 380 390 400
SIDKQDHAAN PCGQIGETVD LDEAVQKALA FARADGNTLV IVTADHAHAS
410 420 430 440 450
QIVSADAKAP GLTQKLTTKD GAPMTLSYGN SEEESQGHTG TQLRVAAYGP
460 470
HAANVVGLTD QTDLFFTMRD AMGIK
Length:475
Mass (Da):49,265
Last modified:November 1, 1990 - v1
Checksum:i1B8B9841CBDE6653
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33965 Genomic DNA. Translation: AAA26563.1.
RefSeqiWP_046686693.1. NZ_JTBU01000001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33965 Genomic DNA. Translation: AAA26563.1.
RefSeqiWP_046686693.1. NZ_JTBU01000001.1.

3D structure databases

ProteinModelPortaliP19147.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB_SERMA
AccessioniPrimary (citable) accession number: P19147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.