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P19146

- ARF2_YEAST

UniProt

P19146 - ARF2_YEAST

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Protein
ADP-ribosylation factor 2
Gene
ARF2, YDL137W, D2165
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481GTP By similarity
Binding sitei70 – 701GTP; via amide nitrogen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328GTP
Nucleotide bindingi126 – 1294GTP
Nucleotide bindingi160 – 1612GTP

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. macroautophagy Source: SGD
  3. protein transport Source: UniProtKB-KW
  4. small GTPase mediated signal transduction Source: InterPro
  5. vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29536-MONOMER.
ReactomeiREACT_188955. Synthesis of PIPs at the Golgi membrane.
REACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 2
Gene namesi
Name:ARF2
Ordered Locus Names:YDL137W
ORF Names:D2165
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

SGDiS000002296. ARF2.

Subcellular locationi

Golgi apparatus 1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 181180ADP-ribosylation factor 2
PRO_0000207419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP19146.
PaxDbiP19146.
PeptideAtlasiP19146.
PRIDEiP19146.

Expressioni

Gene expression databases

GenevestigatoriP19146.

Interactioni

Protein-protein interaction databases

BioGridi31924. 63 interactions.
DIPiDIP-2215N.
IntActiP19146. 19 interactions.
MINTiMINT-671006.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 134
Beta strandi18 – 258
Helixi30 – 378
Beta strandi43 – 486
Beta strandi51 – 588
Beta strandi61 – 688
Helixi76 – 838
Beta strandi86 – 938
Helixi97 – 993
Helixi100 – 11112
Helixi114 – 1163
Beta strandi120 – 1267
Helixi136 – 1438
Helixi145 – 1473
Beta strandi153 – 1575
Turni160 – 1634
Helixi166 – 17611

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MR3X-ray1.60F1-181[»]
ProteinModelPortaliP19146.
SMRiP19146. Positions 5-181.

Miscellaneous databases

EvolutionaryTraceiP19146.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00720000108501.
HOGENOMiHOG000163691.
KOiK07937.
OMAiWLANILK.
OrthoDBiEOG7FNCKB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19146-1 [UniParc]FASTAAdd to Basket

« Hide

MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG    50
FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI 100
GEAREVMQRM LNEDELRNAV WLVFANKQDL PEAMSAAEIT EKLGLHSIRN 150
RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ S 181
Length:181
Mass (Da):20,658
Last modified:January 23, 2007 - v3
Checksum:i7E43FF792E8D64E8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35158 Genomic DNA. Translation: AAA34430.1.
X96876 Genomic DNA. Translation: CAA65622.1.
Z74185 Genomic DNA. Translation: CAA98710.1.
AY693030 Genomic DNA. Translation: AAT93049.1.
EF123146 mRNA. Translation: ABM97490.1.
BK006938 Genomic DNA. Translation: DAA11721.1.
PIRiA36367.
RefSeqiNP_010144.1. NM_001180197.1.

Genome annotation databases

EnsemblFungiiYDL137W; YDL137W; YDL137W.
GeneIDi851418.
KEGGisce:YDL137W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35158 Genomic DNA. Translation: AAA34430.1 .
X96876 Genomic DNA. Translation: CAA65622.1 .
Z74185 Genomic DNA. Translation: CAA98710.1 .
AY693030 Genomic DNA. Translation: AAT93049.1 .
EF123146 mRNA. Translation: ABM97490.1 .
BK006938 Genomic DNA. Translation: DAA11721.1 .
PIRi A36367.
RefSeqi NP_010144.1. NM_001180197.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MR3 X-ray 1.60 F 1-181 [» ]
ProteinModelPortali P19146.
SMRi P19146. Positions 5-181.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31924. 63 interactions.
DIPi DIP-2215N.
IntActi P19146. 19 interactions.
MINTi MINT-671006.

Proteomic databases

MaxQBi P19146.
PaxDbi P19146.
PeptideAtlasi P19146.
PRIDEi P19146.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL137W ; YDL137W ; YDL137W .
GeneIDi 851418.
KEGGi sce:YDL137W.

Organism-specific databases

SGDi S000002296. ARF2.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00720000108501.
HOGENOMi HOG000163691.
KOi K07937.
OMAi WLANILK.
OrthoDBi EOG7FNCKB.

Enzyme and pathway databases

BioCyci YEAST:G3O-29536-MONOMER.
Reactomei REACT_188955. Synthesis of PIPs at the Golgi membrane.
REACT_33101. COPI Mediated Transport.
REACT_94264. Clathrin derived vesicle budding.

Miscellaneous databases

EvolutionaryTracei P19146.
NextBioi 968615.

Gene expression databases

Genevestigatori P19146.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view ]
Pfami PF00025. Arf. 1 hit.
[Graphical view ]
PRINTSi PR00328. SAR1GTPBP.
SMARTi SM00177. ARF. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51417. ARF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes."
    Stearns T., Kahn R.A., Botstein D., Hoyt M.A.
    Mol. Cell. Biol. 10:6690-6699(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
    Woelfl S., Haneman V., Saluz H.P.
    Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-54.
    Strain: ATCC 201390 / BY4743.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Structures of yeast ARF2 and ARL1: distinct roles for the N-terminus in the structure and function of ARF family GTPases."
    Amor J.C., Horton J.R., Zhu X., Wang Y., Sullards C., Ringe D., Cheng X., Kahn R.A.
    J. Biol. Chem. 276:42477-42484(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH GDP.

Entry informationi

Entry nameiARF2_YEAST
AccessioniPrimary (citable) accession number: P19146
Secondary accession number(s): A2TBP3, D6VRL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 25000 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

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