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P19146

- ARF2_YEAST

UniProt

P19146 - ARF2_YEAST

Protein

ADP-ribosylation factor 2

Gene

ARF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481GTPBy similarity
    Binding sitei70 – 701GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi25 – 328GTP
    Nucleotide bindingi126 – 1294GTP
    Nucleotide bindingi160 – 1612GTP

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. macroautophagy Source: SGD
    3. protein transport Source: UniProtKB-KW
    4. small GTPase mediated signal transduction Source: InterPro
    5. vesicle-mediated transport Source: UniProtKB-KW

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29536-MONOMER.
    ReactomeiREACT_188955. Synthesis of PIPs at the Golgi membrane.
    REACT_33101. COPI Mediated Transport.
    REACT_94264. Clathrin derived vesicle budding.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ADP-ribosylation factor 2
    Gene namesi
    Name:ARF2
    Ordered Locus Names:YDL137W
    ORF Names:D2165
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    SGDiS000002296. ARF2.

    Subcellular locationi

    Golgi apparatus 1 Publication

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 181180ADP-ribosylation factor 2PRO_0000207419Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Proteomic databases

    MaxQBiP19146.
    PaxDbiP19146.
    PeptideAtlasiP19146.
    PRIDEiP19146.

    Expressioni

    Gene expression databases

    GenevestigatoriP19146.

    Interactioni

    Protein-protein interaction databases

    BioGridi31924. 63 interactions.
    DIPiDIP-2215N.
    IntActiP19146. 19 interactions.
    MINTiMINT-671006.

    Structurei

    Secondary structure

    1
    181
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 134
    Beta strandi18 – 258
    Helixi30 – 378
    Beta strandi43 – 486
    Beta strandi51 – 588
    Beta strandi61 – 688
    Helixi76 – 838
    Beta strandi86 – 938
    Helixi97 – 993
    Helixi100 – 11112
    Helixi114 – 1163
    Beta strandi120 – 1267
    Helixi136 – 1438
    Helixi145 – 1473
    Beta strandi153 – 1575
    Turni160 – 1634
    Helixi166 – 17611

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MR3X-ray1.60F1-181[»]
    ProteinModelPortaliP19146.
    SMRiP19146. Positions 5-181.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19146.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Arf family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00720000108501.
    HOGENOMiHOG000163691.
    KOiK07937.
    OMAiWLANILK.
    OrthoDBiEOG7FNCKB.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view]
    PfamiPF00025. Arf. 1 hit.
    [Graphical view]
    PRINTSiPR00328. SAR1GTPBP.
    SMARTiSM00177. ARF. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51417. ARF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19146-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG    50
    FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI 100
    GEAREVMQRM LNEDELRNAV WLVFANKQDL PEAMSAAEIT EKLGLHSIRN 150
    RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ S 181
    Length:181
    Mass (Da):20,658
    Last modified:January 23, 2007 - v3
    Checksum:i7E43FF792E8D64E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35158 Genomic DNA. Translation: AAA34430.1.
    X96876 Genomic DNA. Translation: CAA65622.1.
    Z74185 Genomic DNA. Translation: CAA98710.1.
    AY693030 Genomic DNA. Translation: AAT93049.1.
    EF123146 mRNA. Translation: ABM97490.1.
    BK006938 Genomic DNA. Translation: DAA11721.1.
    PIRiA36367.
    RefSeqiNP_010144.1. NM_001180197.1.

    Genome annotation databases

    EnsemblFungiiYDL137W; YDL137W; YDL137W.
    GeneIDi851418.
    KEGGisce:YDL137W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35158 Genomic DNA. Translation: AAA34430.1 .
    X96876 Genomic DNA. Translation: CAA65622.1 .
    Z74185 Genomic DNA. Translation: CAA98710.1 .
    AY693030 Genomic DNA. Translation: AAT93049.1 .
    EF123146 mRNA. Translation: ABM97490.1 .
    BK006938 Genomic DNA. Translation: DAA11721.1 .
    PIRi A36367.
    RefSeqi NP_010144.1. NM_001180197.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MR3 X-ray 1.60 F 1-181 [» ]
    ProteinModelPortali P19146.
    SMRi P19146. Positions 5-181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31924. 63 interactions.
    DIPi DIP-2215N.
    IntActi P19146. 19 interactions.
    MINTi MINT-671006.

    Proteomic databases

    MaxQBi P19146.
    PaxDbi P19146.
    PeptideAtlasi P19146.
    PRIDEi P19146.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL137W ; YDL137W ; YDL137W .
    GeneIDi 851418.
    KEGGi sce:YDL137W.

    Organism-specific databases

    SGDi S000002296. ARF2.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00720000108501.
    HOGENOMi HOG000163691.
    KOi K07937.
    OMAi WLANILK.
    OrthoDBi EOG7FNCKB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29536-MONOMER.
    Reactomei REACT_188955. Synthesis of PIPs at the Golgi membrane.
    REACT_33101. COPI Mediated Transport.
    REACT_94264. Clathrin derived vesicle budding.

    Miscellaneous databases

    EvolutionaryTracei P19146.
    NextBioi 968615.

    Gene expression databases

    Genevestigatori P19146.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR024156. Small_GTPase_ARF.
    IPR006689. Small_GTPase_ARF/SAR.
    [Graphical view ]
    Pfami PF00025. Arf. 1 hit.
    [Graphical view ]
    PRINTSi PR00328. SAR1GTPBP.
    SMARTi SM00177. ARF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51417. ARF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes."
      Stearns T., Kahn R.A., Botstein D., Hoyt M.A.
      Mol. Cell. Biol. 10:6690-6699(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
      Woelfl S., Haneman V., Saluz H.P.
      Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-54.
      Strain: ATCC 201390 / BY4743.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Structures of yeast ARF2 and ARL1: distinct roles for the N-terminus in the structure and function of ARF family GTPases."
      Amor J.C., Horton J.R., Zhu X., Wang Y., Sullards C., Ringe D., Cheng X., Kahn R.A.
      J. Biol. Chem. 276:42477-42484(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH GDP.

    Entry informationi

    Entry nameiARF2_YEAST
    AccessioniPrimary (citable) accession number: P19146
    Secondary accession number(s): A2TBP3, D6VRL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 147 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 25000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3