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Protein

ADP-ribosylation factor 2

Gene

ARF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481GTPBy similarity
Binding sitei70 – 701GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328GTP
Nucleotide bindingi126 – 1294GTP
Nucleotide bindingi160 – 1612GTP

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • macroautophagy Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29536-MONOMER.
ReactomeiREACT_287389. Clathrin derived vesicle budding.
REACT_314440. COPI Mediated Transport.
REACT_318087. Synthesis of PIPs at the Golgi membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 2
Gene namesi
Name:ARF2
Ordered Locus Names:YDL137W
ORF Names:D2165
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL137W.
SGDiS000002296. ARF2.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 181180ADP-ribosylation factor 2PRO_0000207419Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP19146.
PaxDbiP19146.
PeptideAtlasiP19146.
PRIDEiP19146.

Interactioni

Protein-protein interaction databases

BioGridi31924. 65 interactions.
DIPiDIP-2215N.
IntActiP19146. 19 interactions.
MINTiMINT-671006.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 134Combined sources
Beta strandi18 – 258Combined sources
Helixi30 – 378Combined sources
Beta strandi43 – 486Combined sources
Beta strandi51 – 588Combined sources
Beta strandi61 – 688Combined sources
Helixi76 – 838Combined sources
Beta strandi86 – 938Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11112Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1438Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MR3X-ray1.60F1-181[»]
ProteinModelPortaliP19146.
SMRiP19146. Positions 5-181.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19146.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
InParanoidiP19146.
KOiK07937.
OMAiHYFRNTE.
OrthoDBiEOG7FNCKB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG
60 70 80 90 100
FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI
110 120 130 140 150
GEAREVMQRM LNEDELRNAV WLVFANKQDL PEAMSAAEIT EKLGLHSIRN
160 170 180
RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ S
Length:181
Mass (Da):20,658
Last modified:January 23, 2007 - v3
Checksum:i7E43FF792E8D64E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35158 Genomic DNA. Translation: AAA34430.1.
X96876 Genomic DNA. Translation: CAA65622.1.
Z74185 Genomic DNA. Translation: CAA98710.1.
AY693030 Genomic DNA. Translation: AAT93049.1.
EF123146 mRNA. Translation: ABM97490.1.
BK006938 Genomic DNA. Translation: DAA11721.1.
PIRiA36367.
RefSeqiNP_010144.1. NM_001180197.1.

Genome annotation databases

EnsemblFungiiYDL137W; YDL137W; YDL137W.
GeneIDi851418.
KEGGisce:YDL137W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35158 Genomic DNA. Translation: AAA34430.1.
X96876 Genomic DNA. Translation: CAA65622.1.
Z74185 Genomic DNA. Translation: CAA98710.1.
AY693030 Genomic DNA. Translation: AAT93049.1.
EF123146 mRNA. Translation: ABM97490.1.
BK006938 Genomic DNA. Translation: DAA11721.1.
PIRiA36367.
RefSeqiNP_010144.1. NM_001180197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MR3X-ray1.60F1-181[»]
ProteinModelPortaliP19146.
SMRiP19146. Positions 5-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31924. 65 interactions.
DIPiDIP-2215N.
IntActiP19146. 19 interactions.
MINTiMINT-671006.

Proteomic databases

MaxQBiP19146.
PaxDbiP19146.
PeptideAtlasiP19146.
PRIDEiP19146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL137W; YDL137W; YDL137W.
GeneIDi851418.
KEGGisce:YDL137W.

Organism-specific databases

EuPathDBiFungiDB:YDL137W.
SGDiS000002296. ARF2.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
InParanoidiP19146.
KOiK07937.
OMAiHYFRNTE.
OrthoDBiEOG7FNCKB.

Enzyme and pathway databases

BioCyciYEAST:G3O-29536-MONOMER.
ReactomeiREACT_287389. Clathrin derived vesicle budding.
REACT_314440. COPI Mediated Transport.
REACT_318087. Synthesis of PIPs at the Golgi membrane.

Miscellaneous databases

EvolutionaryTraceiP19146.
NextBioi968615.
PROiP19146.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes."
    Stearns T., Kahn R.A., Botstein D., Hoyt M.A.
    Mol. Cell. Biol. 10:6690-6699(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
    Woelfl S., Haneman V., Saluz H.P.
    Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-54.
    Strain: ATCC 201390 / BY4743.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Structures of yeast ARF2 and ARL1: distinct roles for the N-terminus in the structure and function of ARF family GTPases."
    Amor J.C., Horton J.R., Zhu X., Wang Y., Sullards C., Ringe D., Cheng X., Kahn R.A.
    J. Biol. Chem. 276:42477-42484(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH GDP.

Entry informationi

Entry nameiARF2_YEAST
AccessioniPrimary (citable) accession number: P19146
Secondary accession number(s): A2TBP3, D6VRL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 25000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.