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Protein

ADP-ribosylation factor 2

Gene

ARF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48GTPBy similarity1
Binding sitei70GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32GTP8
Nucleotide bindingi126 – 129GTP4
Nucleotide bindingi160 – 161GTP2

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • macroautophagy Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29536-MONOMER.
ReactomeiR-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-421837. Clathrin derived vesicle budding.
R-SCE-5620916. VxPx cargo-targeting to cilium.
R-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-SCE-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 2
Gene namesi
Name:ARF2
Ordered Locus Names:YDL137W
ORF Names:D2165
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL137W.
SGDiS000002296. ARF2.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00002074192 – 181ADP-ribosylation factor 2Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Cross-linki127Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

MaxQBiP19146.
PRIDEiP19146.
TopDownProteomicsiP19146.

PTM databases

iPTMnetiP19146.

Interactioni

Protein-protein interaction databases

BioGridi31924. 65 interactors.
DIPiDIP-2215N.
IntActiP19146. 19 interactors.
MINTiMINT-671006.

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 13Combined sources4
Beta strandi18 – 25Combined sources8
Helixi30 – 37Combined sources8
Beta strandi43 – 48Combined sources6
Beta strandi51 – 58Combined sources8
Beta strandi61 – 68Combined sources8
Helixi76 – 83Combined sources8
Beta strandi86 – 93Combined sources8
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Helixi136 – 143Combined sources8
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 176Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MR3X-ray1.60F1-181[»]
ProteinModelPortaliP19146.
SMRiP19146.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19146.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
InParanoidiP19146.
KOiK07937.
OMAiAMREMAN.
OrthoDBiEOG092C508L.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLYASKLFS NLFGNKEMRI LMVGLDGAGK TTVLYKLKLG EVITTIPTIG
60 70 80 90 100
FNVETVQYKN ISFTVWDVGG QDRIRSLWRH YYRNTEGVIF VIDSNDRSRI
110 120 130 140 150
GEAREVMQRM LNEDELRNAV WLVFANKQDL PEAMSAAEIT EKLGLHSIRN
160 170 180
RPWFIQSTCA TSGEGLYEGL EWLSNNLKNQ S
Length:181
Mass (Da):20,658
Last modified:January 23, 2007 - v3
Checksum:i7E43FF792E8D64E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35158 Genomic DNA. Translation: AAA34430.1.
X96876 Genomic DNA. Translation: CAA65622.1.
Z74185 Genomic DNA. Translation: CAA98710.1.
AY693030 Genomic DNA. Translation: AAT93049.1.
EF123146 mRNA. Translation: ABM97490.1.
BK006938 Genomic DNA. Translation: DAA11721.1.
PIRiA36367.
RefSeqiNP_010144.1. NM_001180197.1.

Genome annotation databases

EnsemblFungiiYDL137W; YDL137W; YDL137W.
GeneIDi851418.
KEGGisce:YDL137W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35158 Genomic DNA. Translation: AAA34430.1.
X96876 Genomic DNA. Translation: CAA65622.1.
Z74185 Genomic DNA. Translation: CAA98710.1.
AY693030 Genomic DNA. Translation: AAT93049.1.
EF123146 mRNA. Translation: ABM97490.1.
BK006938 Genomic DNA. Translation: DAA11721.1.
PIRiA36367.
RefSeqiNP_010144.1. NM_001180197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MR3X-ray1.60F1-181[»]
ProteinModelPortaliP19146.
SMRiP19146.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31924. 65 interactors.
DIPiDIP-2215N.
IntActiP19146. 19 interactors.
MINTiMINT-671006.

PTM databases

iPTMnetiP19146.

Proteomic databases

MaxQBiP19146.
PRIDEiP19146.
TopDownProteomicsiP19146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL137W; YDL137W; YDL137W.
GeneIDi851418.
KEGGisce:YDL137W.

Organism-specific databases

EuPathDBiFungiDB:YDL137W.
SGDiS000002296. ARF2.

Phylogenomic databases

GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
InParanoidiP19146.
KOiK07937.
OMAiAMREMAN.
OrthoDBiEOG092C508L.

Enzyme and pathway databases

BioCyciYEAST:G3O-29536-MONOMER.
ReactomeiR-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-421837. Clathrin derived vesicle budding.
R-SCE-5620916. VxPx cargo-targeting to cilium.
R-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-SCE-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiP19146.
PROiP19146.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARF2_YEAST
AccessioniPrimary (citable) accession number: P19146
Secondary accession number(s): A2TBP3, D6VRL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 169 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 25000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.