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Protein

General amino-acid permease GAP1

Gene

GAP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Permease for various amino acids as well as for GABA. Can also transport L-cysteine and beta-alanine.2 Publications

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: SGD
  • antiporter activity Source: GO_Central
  • L-phenylalanine transmembrane transporter activity Source: CACAO
  • L-proline transmembrane transporter activity Source: SGD
  • polyamine transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transport Source: SGD
  • polyamine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32011-MONOMER.
SABIO-RKP19145.

Protein family/group databases

TCDBi2.A.3.10.2. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
General amino-acid permease GAP1
Gene namesi
Name:GAP1
Ordered Locus Names:YKR039W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR039W.
SGDiS000001747. GAP1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 95CytoplasmicSequence analysisAdd BLAST95
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 121ExtracellularSequence analysis5
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 165CytoplasmicSequence analysisAdd BLAST23
Transmembranei166 – 185HelicalSequence analysisAdd BLAST20
Topological domaini186 – 204ExtracellularSequence analysisAdd BLAST19
Transmembranei205 – 224HelicalSequence analysisAdd BLAST20
Topological domaini225 – 237CytoplasmicSequence analysisAdd BLAST13
Transmembranei238 – 256HelicalSequence analysisAdd BLAST19
Topological domaini257 – 280ExtracellularSequence analysisAdd BLAST24
Transmembranei281 – 298HelicalSequence analysisAdd BLAST18
Topological domaini299 – 321CytoplasmicSequence analysisAdd BLAST23
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 376ExtracellularSequence analysisAdd BLAST34
Transmembranei377 – 396HelicalSequence analysisAdd BLAST20
Topological domaini397 – 421CytoplasmicSequence analysisAdd BLAST25
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 451ExtracellularSequence analysis9
Transmembranei452 – 472HelicalSequence analysisAdd BLAST21
Topological domaini473 – 491CytoplasmicSequence analysisAdd BLAST19
Transmembranei492 – 510HelicalSequence analysisAdd BLAST19
Topological domaini511 – 529ExtracellularSequence analysisAdd BLAST19
Transmembranei530 – 548HelicalSequence analysisAdd BLAST19
Topological domaini549 – 602CytoplasmicSequence analysisAdd BLAST54

GO - Cellular componenti

  • endosome Source: SGD
  • ER to Golgi transport vesicle Source: SGD
  • fungal-type vacuole lumen Source: SGD
  • integral component of plasma membrane Source: SGD
  • multivesicular body Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541511 – 602General amino-acid permease GAP1Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP19145.
PRIDEiP19145.

PTM databases

SwissPalmiP19145.

Interactioni

Protein-protein interaction databases

BioGridi34171. 110 interactors.
DIPiDIP-8010N.
IntActiP19145. 4 interactors.
MINTiMINT-1358192.

Structurei

3D structure databases

ProteinModelPortaliP19145.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP19145.
KOiK16261.
OMAiYGNQTVY.
OrthoDBiEOG092C164R.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTSSYEKN NPDNLKHNGI TIDSEFLTQE PITIPSNGSA VSIDETGSGS
60 70 80 90 100
KWQDFKDSFK RVKPIEVDPN LSEAEKVAII TAQTPLKHHL KNRHLQMIAI
110 120 130 140 150
GGAIGTGLLV GSGTALRTGG PASLLIGWGS TGTMIYAMVM ALGELAVIFP
160 170 180 190 200
ISGGFTTYAT RFIDESFGYA NNFNYMLQWL VVLPLEIVSA SITVNFWGTD
210 220 230 240 250
PKYRDGFVAL FWLAIVIINM FGVKGYGEAE FVFSFIKVIT VVGFIILGII
260 270 280 290 300
LNCGGGPTGG YIGGKYWHDP GAFAGDTPGA KFKGVCSVFV TAAFSFAGSE
310 320 330 340 350
LVGLAASESV EPRKSVPKAA KQVFWRITLF YILSLLMIGL LVPYNDKSLI
360 370 380 390 400
GASSVDAAAS PFVIAIKTHG IKGLPSVVNV VILIAVLSVG NSAIYACSRT
410 420 430 440 450
MVALAEQRFL PEIFSYVDRK GRPLVGIAVT SAFGLIAFVA ASKKEGEVFN
460 470 480 490 500
WLLALSGLSS LFTWGGICIC HIRFRKALAA QGRGLDELSF KSPTGVWGSY
510 520 530 540 550
WGLFMVIIMF IAQFYVAVFP VGDSPSAEGF FEAYLSFPLV MVMYIGHKIY
560 570 580 590 600
KRNWKLFIPA EKMDIDTGRR EVDLDLLKQE IAEEKAIMAT KPRWYRIWNF

WC
Length:602
Mass (Da):65,656
Last modified:June 1, 1994 - v2
Checksum:i5363616447907458
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122Missing in CAA36858 (PubMed:2194797).Curated1
Sequence conflicti189S → A in CAA36858 (PubMed:2194797).Curated1
Sequence conflicti338I → V in CAA36858 (PubMed:2194797).Curated1
Sequence conflicti518V → L in CAA36858 (PubMed:2194797).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52633 Genomic DNA. Translation: CAA36858.1.
Z28264 Genomic DNA. Translation: CAA82113.1.
AY723840 Genomic DNA. Translation: AAU09757.1.
BK006944 Genomic DNA. Translation: DAA09192.1.
PIRiS38111.
RefSeqiNP_012965.3. NM_001179829.3.

Genome annotation databases

EnsemblFungiiYKR039W; YKR039W; YKR039W.
GeneIDi853912.
KEGGisce:YKR039W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52633 Genomic DNA. Translation: CAA36858.1.
Z28264 Genomic DNA. Translation: CAA82113.1.
AY723840 Genomic DNA. Translation: AAU09757.1.
BK006944 Genomic DNA. Translation: DAA09192.1.
PIRiS38111.
RefSeqiNP_012965.3. NM_001179829.3.

3D structure databases

ProteinModelPortaliP19145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34171. 110 interactors.
DIPiDIP-8010N.
IntActiP19145. 4 interactors.
MINTiMINT-1358192.

Protein family/group databases

TCDBi2.A.3.10.2. the amino acid-polyamine-organocation (apc) family.

PTM databases

SwissPalmiP19145.

Proteomic databases

MaxQBiP19145.
PRIDEiP19145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR039W; YKR039W; YKR039W.
GeneIDi853912.
KEGGisce:YKR039W.

Organism-specific databases

EuPathDBiFungiDB:YKR039W.
SGDiS000001747. GAP1.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP19145.
KOiK16261.
OMAiYGNQTVY.
OrthoDBiEOG092C164R.

Enzyme and pathway databases

BioCyciYEAST:G3O-32011-MONOMER.
SABIO-RKP19145.

Miscellaneous databases

PROiP19145.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAP1_YEAST
AccessioniPrimary (citable) accession number: P19145
Secondary accession number(s): D6VXA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.