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Protein

General amino-acid permease GAP1

Gene

GAP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General amino-acid permease involved in the uptake of all the naturally occurring L-amino-acids, related compounds such as ornithine and citrulline, some D-amino acids, toxic amino acid analogs such as azetidine-2-carboxylate, and the polyamines putrescine and spermidine (PubMed:5474888, PubMed:10467005, PubMed:10373490, PubMed:10953083, PubMed:14968425, PubMed:15707981). Senses its transport substrates to set an appropriate level of transporter activity at the cell surface (PubMed:21471002). Required for FLO11 expression and invasive growth (PubMed:22844449).8 Publications

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: SGD
  • L-phenylalanine transmembrane transporter activity Source: CACAO
  • L-proline transmembrane transporter activity Source: SGD
  • polyamine transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transport Source: SGD
  • polyamine transport Source: SGD

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32011-MONOMER
SABIO-RKiP19145

Protein family/group databases

TCDBi2.A.3.10.2 the amino acid-polyamine-organocation (apc) family

Names & Taxonomyi

Protein namesi
Recommended name:
General amino-acid permease GAP11 Publication
Gene namesi
Name:GAP11 Publication
Ordered Locus Names:YKR039W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR039W
SGDiS000001747 GAP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 95CytoplasmicSequence analysisAdd BLAST95
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 121ExtracellularSequence analysis5
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 165CytoplasmicSequence analysisAdd BLAST23
Transmembranei166 – 185HelicalSequence analysisAdd BLAST20
Topological domaini186 – 204ExtracellularSequence analysisAdd BLAST19
Transmembranei205 – 224HelicalSequence analysisAdd BLAST20
Topological domaini225 – 237CytoplasmicSequence analysisAdd BLAST13
Transmembranei238 – 256HelicalSequence analysisAdd BLAST19
Topological domaini257 – 280ExtracellularSequence analysisAdd BLAST24
Transmembranei281 – 298HelicalSequence analysisAdd BLAST18
Topological domaini299 – 321CytoplasmicSequence analysisAdd BLAST23
Transmembranei322 – 342HelicalSequence analysisAdd BLAST21
Topological domaini343 – 376ExtracellularSequence analysisAdd BLAST34
Transmembranei377 – 396HelicalSequence analysisAdd BLAST20
Topological domaini397 – 421CytoplasmicSequence analysisAdd BLAST25
Transmembranei422 – 442HelicalSequence analysisAdd BLAST21
Topological domaini443 – 451ExtracellularSequence analysis9
Transmembranei452 – 472HelicalSequence analysisAdd BLAST21
Topological domaini473 – 491CytoplasmicSequence analysisAdd BLAST19
Transmembranei492 – 510HelicalSequence analysisAdd BLAST19
Topological domaini511 – 529ExtracellularSequence analysisAdd BLAST19
Transmembranei530 – 548HelicalSequence analysisAdd BLAST19
Topological domaini549 – 602CytoplasmicSequence analysisAdd BLAST54

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi297A → V: Impairs basic amino-acids transport and regulation by these amino-acids. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541511 – 602General amino-acid permease GAP1Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications

Post-translational modificationi

Active permease is phosphorylated (PubMed:7798155). The addition of glutamine causes rapid dephosphorylation and inactivation of the permease (PubMed:7798155).1 Publication
Ubiquitination by RSP5 and the RSP5-associated proteins BUL1 and BUL2, leads the addition of poly-ubiquitin chains being specifically formed by linkage through the lysine 63 residue of ubiquitin and mediates ammonium-induced endocytosis and degradation in the vacuole (PubMed:9614172, PubMed:10194416, PubMed:11500494, PubMed:11352928, PubMed:14523026, PubMed:15039776).6 Publications

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP19145
PaxDbiP19145
PRIDEiP19145

PTM databases

iPTMnetiP19145
SwissPalmiP19145

Expressioni

Inductioni

Expression is repressed by ammonia (PubMed:2194797). Expression is induced by polyamines (PubMed:15707981). Both GLN3 and NIL1 bind the 5'-GATAAG-3' motif within the GAP1 promoter to activate transcription (PubMed:8636059).3 Publications

Interactioni

Protein-protein interaction databases

BioGridi34171, 166 interactors
DIPiDIP-8010N
IntActiP19145, 5 interactors
MINTiP19145
STRINGi4932.YKR039W

Structurei

3D structure databases

ProteinModelPortaliP19145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744
HOGENOMiHOG000261848
InParanoidiP19145
KOiK16261
OMAiPAWIPGI
OrthoDBiEOG092C164R

Family and domain databases

InterProiView protein in InterPro
IPR004841 AA-permease/SLC12A_dom
IPR002293 AA/rel_permease1
IPR004762 Amino_acid_permease_fungi
IPR004840 Amoino_acid_permease_CS
PfamiView protein in Pfam
PF00324 AA_permease, 1 hit
PIRSFiPIRSF006060 AA_transporter, 1 hit
TIGRFAMsiTIGR00913 2A0310, 1 hit
PROSITEiView protein in PROSITE
PS00218 AMINO_ACID_PERMEASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P19145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTSSYEKN NPDNLKHNGI TIDSEFLTQE PITIPSNGSA VSIDETGSGS
60 70 80 90 100
KWQDFKDSFK RVKPIEVDPN LSEAEKVAII TAQTPLKHHL KNRHLQMIAI
110 120 130 140 150
GGAIGTGLLV GSGTALRTGG PASLLIGWGS TGTMIYAMVM ALGELAVIFP
160 170 180 190 200
ISGGFTTYAT RFIDESFGYA NNFNYMLQWL VVLPLEIVSA SITVNFWGTD
210 220 230 240 250
PKYRDGFVAL FWLAIVIINM FGVKGYGEAE FVFSFIKVIT VVGFIILGII
260 270 280 290 300
LNCGGGPTGG YIGGKYWHDP GAFAGDTPGA KFKGVCSVFV TAAFSFAGSE
310 320 330 340 350
LVGLAASESV EPRKSVPKAA KQVFWRITLF YILSLLMIGL LVPYNDKSLI
360 370 380 390 400
GASSVDAAAS PFVIAIKTHG IKGLPSVVNV VILIAVLSVG NSAIYACSRT
410 420 430 440 450
MVALAEQRFL PEIFSYVDRK GRPLVGIAVT SAFGLIAFVA ASKKEGEVFN
460 470 480 490 500
WLLALSGLSS LFTWGGICIC HIRFRKALAA QGRGLDELSF KSPTGVWGSY
510 520 530 540 550
WGLFMVIIMF IAQFYVAVFP VGDSPSAEGF FEAYLSFPLV MVMYIGHKIY
560 570 580 590 600
KRNWKLFIPA EKMDIDTGRR EVDLDLLKQE IAEEKAIMAT KPRWYRIWNF

WC
Length:602
Mass (Da):65,656
Last modified:June 1, 1994 - v2
Checksum:i5363616447907458
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122Missing in CAA36858 (PubMed:2194797).Curated1
Sequence conflicti189S → A in CAA36858 (PubMed:2194797).Curated1
Sequence conflicti338I → V in CAA36858 (PubMed:2194797).Curated1
Sequence conflicti518V → L in CAA36858 (PubMed:2194797).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52633 Genomic DNA Translation: CAA36858.1
Z28264 Genomic DNA Translation: CAA82113.1
AY723840 Genomic DNA Translation: AAU09757.1
BK006944 Genomic DNA Translation: DAA09192.1
PIRiS38111
RefSeqiNP_012965.3, NM_001179829.3

Genome annotation databases

EnsemblFungiiYKR039W; YKR039W; YKR039W
GeneIDi853912
KEGGisce:YKR039W

Similar proteinsi

Entry informationi

Entry nameiGAP1_YEAST
AccessioniPrimary (citable) accession number: P19145
Secondary accession number(s): D6VXA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 1, 1994
Last modified: March 28, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health