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P19145 (GAP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
General amino-acid permease GAP1
Gene names
Name:GAP1
Ordered Locus Names:YKR039W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Permease for various amino acids as well as for GABA. Can also transport L-cysteine and beta-alanine. Ref.5 Ref.6

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602General amino-acid permease GAP1
PRO_0000054151

Regions

Topological domain1 – 9595Cytoplasmic Potential
Transmembrane96 – 11621Helical; Potential
Topological domain117 – 1215Extracellular Potential
Transmembrane122 – 14221Helical; Potential
Topological domain143 – 16523Cytoplasmic Potential
Transmembrane166 – 18520Helical; Potential
Topological domain186 – 20419Extracellular Potential
Transmembrane205 – 22420Helical; Potential
Topological domain225 – 23713Cytoplasmic Potential
Transmembrane238 – 25619Helical; Potential
Topological domain257 – 28024Extracellular Potential
Transmembrane281 – 29818Helical; Potential
Topological domain299 – 32123Cytoplasmic Potential
Transmembrane322 – 34221Helical; Potential
Topological domain343 – 37634Extracellular Potential
Transmembrane377 – 39620Helical; Potential
Topological domain397 – 42125Cytoplasmic Potential
Transmembrane422 – 44221Helical; Potential
Topological domain443 – 4519Extracellular Potential
Transmembrane452 – 47221Helical; Potential
Topological domain473 – 49119Cytoplasmic Potential
Transmembrane492 – 51019Helical; Potential
Topological domain511 – 52919Extracellular Potential
Transmembrane530 – 54819Helical; Potential
Topological domain549 – 60254Cytoplasmic Potential

Amino acid modifications

Cross-link76Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7 Ref.8

Experimental info

Sequence conflict1221Missing in CAA36858. Ref.1
Sequence conflict1891S → A in CAA36858. Ref.1
Sequence conflict3381I → V in CAA36858. Ref.1
Sequence conflict5181V → L in CAA36858. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P19145 [UniParc].

Last modified June 1, 1994. Version 2.
Checksum: 5363616447907458

FASTA60265,656
        10         20         30         40         50         60 
MSNTSSYEKN NPDNLKHNGI TIDSEFLTQE PITIPSNGSA VSIDETGSGS KWQDFKDSFK 

        70         80         90        100        110        120 
RVKPIEVDPN LSEAEKVAII TAQTPLKHHL KNRHLQMIAI GGAIGTGLLV GSGTALRTGG 

       130        140        150        160        170        180 
PASLLIGWGS TGTMIYAMVM ALGELAVIFP ISGGFTTYAT RFIDESFGYA NNFNYMLQWL 

       190        200        210        220        230        240 
VVLPLEIVSA SITVNFWGTD PKYRDGFVAL FWLAIVIINM FGVKGYGEAE FVFSFIKVIT 

       250        260        270        280        290        300 
VVGFIILGII LNCGGGPTGG YIGGKYWHDP GAFAGDTPGA KFKGVCSVFV TAAFSFAGSE 

       310        320        330        340        350        360 
LVGLAASESV EPRKSVPKAA KQVFWRITLF YILSLLMIGL LVPYNDKSLI GASSVDAAAS 

       370        380        390        400        410        420 
PFVIAIKTHG IKGLPSVVNV VILIAVLSVG NSAIYACSRT MVALAEQRFL PEIFSYVDRK 

       430        440        450        460        470        480 
GRPLVGIAVT SAFGLIAFVA ASKKEGEVFN WLLALSGLSS LFTWGGICIC HIRFRKALAA 

       490        500        510        520        530        540 
QGRGLDELSF KSPTGVWGSY WGLFMVIIMF IAQFYVAVFP VGDSPSAEGF FEAYLSFPLV 

       550        560        570        580        590        600 
MVMYIGHKIY KRNWKLFIPA EKMDIDTGRR EVDLDLLKQE IAEEKAIMAT KPRWYRIWNF 


WC

« Hide

References

« Hide 'large scale' references
[1]"GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression."
Jauniaux J.-C., Grenson M.
Eur. J. Biochem. 190:39-44(1990) [PubMed: 2194797] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases."
During-Olsen L., Regenberg B., Gjermansen C., Kielland-Brandt M.C., Hansen J.
Curr. Genet. 35:609-617(1999) [PubMed: 10467005] [Abstract]
Cited for: FUNCTION IN L-CYSTEINE UPTAKE.
[6]"The fenpropimorph resistance gene FEN2 from Saccharomyces cerevisiae encodes a plasma membrane H+-pantothenate symporter."
Stolz J., Sauer N.
J. Biol. Chem. 274:18747-18752(1999) [PubMed: 10373490] [Abstract]
Cited for: FUNCTION IN BETA-ALANINE UPTAKE.
[7]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-76, MASS SPECTROMETRY.
Strain: SUB592.
[8]"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock A.L., Auld K., Gygi S.P., Silver P.A.
Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-76, MASS SPECTROMETRY.
[9]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X52633 Genomic DNA. Translation: CAA36858.1.
Z28264 Genomic DNA. Translation: CAA82113.1.
AY723840 Genomic DNA. Translation: AAU09757.1.
BK006944 Genomic DNA. Translation: DAA09192.1.
PIRS38111.
RefSeqNP_012965.1. NM_001179829.1.

3D structure databases

ProteinModelPortalP19145.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-8010N.
IntActP19145. 142 interactions.
MINTMINT-1358192.
STRINGP19145.

Protein family/group databases

TCDB2.A.3.10.2. amino acid-polyamine-organocation (APC) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKR039W; YKR039W; YKR039W.
GeneID853912.
KEGGsce:YKR039W.
NMPDRfig|4932.3.peg.3951.

Organism-specific databases

CYGDYKR039w.
SGDS000001747. GAP1.

Phylogenomic databases

eggNOGfuNOG05282.
GeneTreeEFGT00050000001131.
HOGENOMHBG492579.
OMAGEVFNWL.
OrthoDBEOG4WHCV6.

Gene expression databases

ArrayExpressP19145.
GenevestigatorP19145.
GermOnlineYKR039W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERPTHR11785. AA/rel_permease1. 1 hit.
PfamPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00913. 2A0310. 1 hit.
PROSITEPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975250.

Entry information

Entry nameGAP1_YEAST
AccessionPrimary (citable) accession number: P19145
Secondary accession number(s): D6VXA2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: June 1, 1994
Last modified: December 14, 2011
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents