Reviewed, UniProtKB/Swiss-Prot P19139 (CSK21_RAT)

Names and origin

Protein names

Recommended name:
    Casein kinase II subunit alpha
      Short name=CK II
    EC=2.7.11.1

Gene names

Name:

Csnk2a1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-390' of p53/TP53 following UV irradiation By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Tetramer composed of an alpha chain, an alpha' and two beta chains. Interacts with RNPS1. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell growth Ref.1 Non-traceable author statement. Source: RGD regulation of cell proliferation Ref.1 Non-traceable author statement. Source: RGD
Cellular component	protein kinase CK2 complex Ref.1 Traceable author statement. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 391

391

Casein kinase II subunit alpha

PRO_0000085886

Regions

Domain

39 – 324

286

Protein kinase

Nucleotide binding

45 – 53

9

ATP By similarity

Sites

Active site

156

1

Proton acceptor By similarity

Binding site

68

1

ATP By similarity

Amino acid modifications

Modified residue

2

1

Phosphoserine By similarity

Modified residue

102

1

N6-acetyllysine By similarity

Modified residue

194

1

Phosphoserine By similarity

Modified residue

362

1

Phosphoserine By similarity

Modified residue

370

1

Phosphoserine By similarity

Secondary structure

1

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391

Helix Strand Turn

Details...

Beta strand

10 – 12

3

Turn

15 – 18

4

Helix

21 – 24

4

Helix

36 – 38

3

Beta strand

39 – 43

5

Beta strand

52 – 58

7

Turn

59 – 61

3

Beta strand

64 – 69

6

Helix

75 – 88

14

Beta strand

97 – 102

6

Beta strand

109 – 114

6

Helix

122 – 125

4

Helix

130 – 149

20

Helix

159 – 161

3

Beta strand

162 – 165

4

Turn

166 – 169

4

Beta strand

170 – 173

4

Helix

195 – 197

3

Turn

201 – 205

5

Helix

212 – 217

6

Helix

221 – 226

6

Beta strand

230 – 232

3

Helix

240 – 246

7

Helix

252 – 260

9

Helix

267 – 271

5

Helix

281 – 284

4

Turn

287 – 292

6

Helix

295 – 304

10

Helix

309 – 311

3

Helix

315 – 319

5

Helix

322 – 324

3

Helix

325 – 328

4

Sequences

	Sequence		Length	Mass (Da)
	P19139-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 83A4E2CC80824F66	FASTA	391	45,073
	10 20 30 40 50 60 MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY SEVFEAINIT 70 80 90 100 110 120 NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD 130 140 150 160 170 180 FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE 190 200 210 220 230 240 FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD 250 260 270 280 290 300 QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF 310 320 330 340 350 360 LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMSSAGMA GGSTPVSSAN MMSGISSVPT 370 380 390 PSPLGPLAGS PVIAAANSLG IPVPAAAGAQ Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNAs encoding the alpha and beta subunits of rat casein kinase 2 (CK-2): investigation of molecular regulation of CK-2 by androgens in rat ventral prostate." Ahmed K., Davis A., Hanten J., Lambert D., McIvor R.S., Goueli S.A. Cell. Mol. Biol. Res. 39:451-462(1993) [PubMed: 8173590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[3]	"Molecular cloning of the human casein kinase II alpha subunit." Meisner H., Heller-Harrison R., Buxton J., Czech M.P. Biochemistry 28:4072-4076(1989) [PubMed: 2752008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-391.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L15618 mRNA. Translation: AAA74462.1.
BC091130 mRNA. Translation: AAH91130.1.
J02853 mRNA. No translation available.

IPI

IPI00192586.

PIR

B30319.

RefSeq

NP_446276.1.

UniGene

Rn.4231

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2R7I	X-ray	3.00	A/B/C/D	1-335	[»]

SMR

P19139. Positions 2-334.

ModBase

Search...

Protein-protein interaction databases

STRING

P19139.

PTM databases

PhosphoSite

P19139.

Genome annotation databases

Ensembl

ENSRNOT00000007558; ENSRNOP00000007558; ENSRNOG00000005276; Rattus norvegicus. [Genome view]

GeneID

116549.

KEGG

rno:116549.

UCSC

NM_053824. rat.

Organism-specific databases

CTD

116549.

RGD

621663. Csnk2a1.

Phylogenomic databases

HOVERGEN

P19139.

OMA

ALIFEHV.

Enzyme and pathway databases

BRENDA

2.7.11.1. 248.

Gene expression databases

ArrayExpress

P19139.

Genevestigator

P19139.

GermOnline

ENSRNOG00000005276. Rattus norvegicus.

Family and domain databases

InterPro

IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
[Graphical view]

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

619175.

Entry information

Entry name

CSK21_RAT

Accession

Primary (citable) accession number: P19139
Secondary accession number(s): Q5BKC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)