P19125 (NDHJ_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD(P)H-quinone oxidoreductase subunit J
EC=1.6.5.-

Alternative name(s):
NAD(P)H dehydrogenase subunit J
NADH-plastoquinone oxidoreductase subunit J
NDH-1 subunit J
Short name=NDH-J
ORF 155

Gene names

Name:	ndhJ
Ordered Locus Names:	slr1281

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration By similarity. HAMAP MF_01357
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01357
Subunit structure	NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions.
Subcellular location	Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side Probable Ref.6.
Post-translational modification	In at one experiment the initiator methionine has been seen to be kept and removed (Ref.4). HAMAP MF_01357
Sequence similarities	Belongs to the complex I 30 kDa subunit family.
Sequence caution	The sequence CAA35486.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Thylakoid
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; partial Ref.3
	Chain	2 – 179	178	NAD(P)H-quinone oxidoreductase subunit J HAMAP MF_01357		PRO_0000118667

Sequences

	Sequence		Length	Mass (Da)
	P19125 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 3EE18D5E727F8BCC	FASTA	179	20,593
	10 20 30 40 50 60 MAEEVNSPNE AVNLQEETAI APVGPVSTWL TTNGFEHQSL TADHLGVEMV QVEADLLLPL 70 80 90 100 110 120 CTALYAYGFN YLQCQGAYDE GPGKSLVSFY HLVKLTEDTR NPEEVRLKVF LPRENPVVPS 130 140 150 160 170 VYWIWKAADW QERECYDMFG IVYEGHPNLK RILMPEDWVG WPLRKDYISP DFYELQDAY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the ndhC-psbG-ORF157/159 operon of maize plastid DNA and of the cyanobacterium Synechocystis sp. PCC6803." Steinmueller K., Ley A.C., Steinmetz A.A., Sayre R.T., Bogorad L. Mol. Gen. Genet. 216:60-69(1989) [PubMed: 2499764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[3]	"Immunopurification of a subcomplex of the NAD(P)H-plastoquinone-oxidoreductase from the cyanobacterium Synechocystis sp. PCC6803." Berger S., Ellersiek U., Kinzelt D., Steinmueller K. FEBS Lett. 326:246-250(1993) [PubMed: 8325373] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20 AND 23-40, CHARACTERIZATION AS A MEMBER OF THE NAD(P)H-QUINONE OXIDOREDUCTASE COMPLEX.
[4]	"Subunit composition of NDH-1 complexes of Synechocystis sp. PCC 6803: identification of two new ndh gene products with nuclear-encoded homologues in the chloroplast Ndh complex." Prommeenate P., Lennon A.M., Markert C., Hippler M., Nixon P.J. J. Biol. Chem. 279:28165-28173(2004) [PubMed: 15102833] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9, CHARACTERIZATION AS A MEMBER OF THE NAD(P)H-QUINONE OXIDOREDUCTASE COMPLEX, SUBCOMPLEXES OF NDH-1.
[5]	"Identification of NdhL and Ssl1690 (NdhO) in NDH-1L and NDH-1M complexes of Synechocystis sp. PCC 6803." Battchikova N., Zhang P., Rudd S., Ogawa T., Aro E.-M. J. Biol. Chem. 280:2587-2595(2005) [PubMed: 15548534] [Abstract] Cited for: PROTEIN SEQUENCE OF 85-94; 109-126 AND 152-164, SUBCOMPLEXES OF NDH-1.
[6]	"Proteomic studies of the thylakoid membrane of Synechocystis sp. PCC 6803." Srivastava R., Pisareva T., Norling B. Proteomics 5:4905-4916(2005) [PubMed: 16287171] [Abstract] Cited for: SUBCELLULAR LOCATION IN THYLAKOID.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17439 Genomic DNA. Translation: CAA35486.1. Different initiation. BA000022 Genomic DNA. Translation: BAA18285.1.
PIR	S75826.
RefSeq	NP_441605.1. NC_000911.1.
3D structure databases
ProteinModelPortal	P19125.
ModBase	Search...
Protein-protein interaction databases
IntAct	P19125. 11 interactions.
STRING	P19125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	953071.
GenomeReviews	Gene locus slr1281 in contig BA000022_GR.
KEGG	syn:slr1281.
NMPDR	fig|1148.1.peg.1706.
PATRIC	23840649. VBISynSp132158_1879.
Phylogenomic databases
eggNOG	COG0852.
HOGENOM	HBG727675.
OMA	YELQDAY.
PhylomeDB	P19125.
ProtClustDB	PRK12494.
Enzyme and pathway databases
BioCyc	SSP1148:SLR1281-MONOMER.
Family and domain databases
HAMAP	MF_01357. NDH1_NuoC. [Tree]
InterPro	IPR010218. NADH_DH_suC. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
KO	K05581.
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00542. COMPLEX1_30K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDHJ_SYNY3

Accession

Primary (citable) accession number: P19125
Secondary accession number(s): P74194

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 89 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program