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Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611MagnesiumBy similarity
Metal bindingi61 – 611Zinc 1By similarity
Active sitei111 – 1111Phosphoserine intermediatePROSITE-ProRule annotation1 Publication
Metal bindingi111 – 1111Zinc 1By similarity
Metal bindingi174 – 1741MagnesiumBy similarity
Metal bindingi330 – 3301MagnesiumBy similarity
Metal bindingi335 – 3351Zinc 2By similarity
Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
Metal bindingi376 – 3761Zinc 1By similarity
Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
Metal bindingi451 – 4511Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

SABIO-RKP19111.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Gene namesi
Name:ALPI
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19192 PublicationsAdd
BLAST
Chaini20 – 506487Intestinal-type alkaline phosphatasePRO_0000024035Add
BLAST
Propeptidei507 – 53327Removed in mature formBy similarityPRO_0000024036Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi140 ↔ 202By similarity
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi486 ↔ 493By similarity
Lipidationi506 – 5061GPI-anchor amidated aspartateBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PRIDEiP19111.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP19111. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP19111.
SMRiP19111. Positions 20-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP19111.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGACVLLLL GLHLQLSLGL VPVEEEDPAF WNRQAAQALD VAKKLQPIQT
60 70 80 90 100
AAKNVILFLG DGMGVPTVTA TRILKGQMNG KLGPETPLAM DQFPYVALSK
110 120 130 140 150
TYNVDRQVPD SAGTATAYLC GVKGNYRTIG VSAAARYNQC KTTRGNEVTS
160 170 180 190 200
VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADLPADAQMN
210 220 230 240 250
GCQDIAAQLV NNMDIDVILG GGRKYMFPVG TPDPEYPDDA SVNGVRKRKQ
260 270 280 290 300
NLVQAWQAKH QGAQYVWNRT ALLQAADDSS VTHLMGLFEP ADMKYNVQQD
310 320 330 340 350
HTKDPTLQEM TEVALRVVSR NPRGFYLFVE GGRIDHGHHD DKAYMALTEA
360 370 380 390 400
GMFDNAIAKA NELTSELDTL ILVTADHSHV FSFGGYTLRG TSIFGLAPSK
410 420 430 440 450
ALDSKSYTSI LYGNGPGYAL GGGSRPDVND STSEDPSYQQ QAAVPQASET
460 470 480 490 500
HGGEDVAVFA RGPQAHLVHG VEEETFVAHI MAFAGCVEPY TDCNLPAPTT
510 520 530
ATSIPDAAHL AASPPPLALL AGAMLLLLAP TLY
Length:533
Mass (Da):57,100
Last modified:November 1, 1997 - v2
Checksum:iD74456F3F1F06714
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1503VTS → TSV AA sequence (PubMed:3902089).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07733 Genomic DNA. Translation: AAA30571.1.
PIRiS30364.
UniGeneiBt.106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07733 Genomic DNA. Translation: AAA30571.1.
PIRiS30364.
UniGeneiBt.106.

3D structure databases

ProteinModelPortaliP19111.
SMRiP19111. Positions 20-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19111. 2 interactions.

Chemistry

BindingDBiP19111.
ChEMBLiCHEMBL5695.

Proteomic databases

PRIDEiP19111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP19111.

Enzyme and pathway databases

SABIO-RKP19111.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the bovine intestinal alkaline phosphatase gene: biochemical characterization of the recombinant enzyme."
    Weissig H., Schildge A., Hoylaerts M.F., Iqbal M., Millan J.L.
    Biochem. J. 290:503-508(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  2. "The active-site and amino-terminal amino acid sequence of bovine intestinal alkaline phosphatase."
    Culp J.S., Hermodson M., Butler L.G.
    Biochim. Biophys. Acta 831:330-334(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-35 AND 91-152, ACTIVE SITE.
    Tissue: Intestine.
  3. "Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
    Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
    Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-63.

Entry informationi

Entry nameiPPBI_BOVIN
AccessioniPrimary (citable) accession number: P19111
Secondary accession number(s): Q28124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.