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P19111 (PPBI_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Intestinal-type alkaline phosphatase
Short name=IAP
Short name=Intestinal alkaline phosphatase
EC=3.1.3.1
Gene names
Name:ALPI
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.2 Ref.3
Chain20 – 506487Intestinal-type alkaline phosphatase
PRO_0000024035
Propeptide507 – 53327Removed in mature form By similarity
PRO_0000024036

Sites

Active site1111Phosphoserine intermediate Ref.2
Metal binding611Magnesium Potential
Metal binding611Zinc 2 Potential
Metal binding3301Magnesium Potential
Metal binding3351Zinc 1 Potential
Metal binding3391Zinc 1 Potential
Metal binding3761Zinc 2 Potential
Metal binding3771Zinc 2 Potential
Metal binding4511Zinc 1 Potential

Amino acid modifications

Lipidation5061GPI-anchor amidated aspartate By similarity
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation4291N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 202 By similarity
Disulfide bond486 ↔ 493 By similarity

Experimental info

Sequence conflict148 – 1503VTS → TSV AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P19111 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: D74456F3F1F06714

FASTA53357,100
        10         20         30         40         50         60 
MQGACVLLLL GLHLQLSLGL VPVEEEDPAF WNRQAAQALD VAKKLQPIQT AAKNVILFLG 

        70         80         90        100        110        120 
DGMGVPTVTA TRILKGQMNG KLGPETPLAM DQFPYVALSK TYNVDRQVPD SAGTATAYLC 

       130        140        150        160        170        180 
GVKGNYRTIG VSAAARYNQC KTTRGNEVTS VMNRAKKAGK SVGVVTTTRV QHASPAGAYA 

       190        200        210        220        230        240 
HTVNRNWYSD ADLPADAQMN GCQDIAAQLV NNMDIDVILG GGRKYMFPVG TPDPEYPDDA 

       250        260        270        280        290        300 
SVNGVRKRKQ NLVQAWQAKH QGAQYVWNRT ALLQAADDSS VTHLMGLFEP ADMKYNVQQD 

       310        320        330        340        350        360 
HTKDPTLQEM TEVALRVVSR NPRGFYLFVE GGRIDHGHHD DKAYMALTEA GMFDNAIAKA 

       370        380        390        400        410        420 
NELTSELDTL ILVTADHSHV FSFGGYTLRG TSIFGLAPSK ALDSKSYTSI LYGNGPGYAL 

       430        440        450        460        470        480 
GGGSRPDVND STSEDPSYQQ QAAVPQASET HGGEDVAVFA RGPQAHLVHG VEEETFVAHI 

       490        500        510        520        530 
MAFAGCVEPY TDCNLPAPTT ATSIPDAAHL AASPPPLALL AGAMLLLLAP TLY

« Hide

References

[1]"Cloning and expression of the bovine intestinal alkaline phosphatase gene: biochemical characterization of the recombinant enzyme."
Weissig H., Schildge A., Hoylaerts M.F., Iqbal M., Millan J.L.
Biochem. J. 290:503-508(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[2]"The active-site and amino-terminal amino acid sequence of bovine intestinal alkaline phosphatase."
Culp J.S., Hermodson M., Butler L.G.
Biochim. Biophys. Acta 831:330-334(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-35 AND 91-152, ACTIVE SITE.
Tissue: Intestine.
[3]"Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-63.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L07733 Genomic DNA. Translation: AAA30571.1.
IPIIPI00710282.
PIRS30364.
UniGeneBt.106.

3D structure databases

ProteinModelPortalP19111.
SMRP19111. Positions 20-498.
ModBaseSearch...

Protein-protein interaction databases

IntActP19111. 1 interaction.

Proteomic databases

PRIDEP19111.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidP19111.
OrthoDBEOG4GB765.

Enzyme and pathway databases

SABIO-RKP19111.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP19111.
ChEMBLCHEMBL5695.

Entry information

Entry namePPBI_BOVIN
AccessionPrimary (citable) accession number: P19111
Secondary accession number(s): Q28124
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: April 3, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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