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P19111

- PPBI_BOVIN

UniProt

P19111 - PPBI_BOVIN

Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611MagnesiumBy similarity
    Metal bindingi61 – 611Zinc 1By similarity
    Active sitei111 – 1111Phosphoserine intermediate1 PublicationPROSITE-ProRule annotation
    Metal bindingi111 – 1111Zinc 1By similarity
    Metal bindingi174 – 1741MagnesiumBy similarity
    Metal bindingi330 – 3301MagnesiumBy similarity
    Metal bindingi335 – 3351Zinc 2By similarity
    Metal bindingi339 – 3391Zinc 2; via tele nitrogenBy similarity
    Metal bindingi376 – 3761Zinc 1By similarity
    Metal bindingi377 – 3771Zinc 1; via tele nitrogenBy similarity
    Metal bindingi451 – 4511Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP19111.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Intestinal-type alkaline phosphatase (EC:3.1.3.1)
    Short name:
    IAP
    Short name:
    Intestinal alkaline phosphatase
    Gene namesi
    Name:ALPI
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19192 PublicationsAdd
    BLAST
    Chaini20 – 506487Intestinal-type alkaline phosphatasePRO_0000024035Add
    BLAST
    Propeptidei507 – 53327Removed in mature formBy similarityPRO_0000024036Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi140 ↔ 202By similarity
    Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi429 – 4291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi486 ↔ 493By similarity
    Lipidationi506 – 5061GPI-anchor amidated aspartateBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP19111.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP19111. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP19111.
    SMRiP19111. Positions 20-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal, Transmembrane

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiP19111.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19111-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGACVLLLL GLHLQLSLGL VPVEEEDPAF WNRQAAQALD VAKKLQPIQT    50
    AAKNVILFLG DGMGVPTVTA TRILKGQMNG KLGPETPLAM DQFPYVALSK 100
    TYNVDRQVPD SAGTATAYLC GVKGNYRTIG VSAAARYNQC KTTRGNEVTS 150
    VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADLPADAQMN 200
    GCQDIAAQLV NNMDIDVILG GGRKYMFPVG TPDPEYPDDA SVNGVRKRKQ 250
    NLVQAWQAKH QGAQYVWNRT ALLQAADDSS VTHLMGLFEP ADMKYNVQQD 300
    HTKDPTLQEM TEVALRVVSR NPRGFYLFVE GGRIDHGHHD DKAYMALTEA 350
    GMFDNAIAKA NELTSELDTL ILVTADHSHV FSFGGYTLRG TSIFGLAPSK 400
    ALDSKSYTSI LYGNGPGYAL GGGSRPDVND STSEDPSYQQ QAAVPQASET 450
    HGGEDVAVFA RGPQAHLVHG VEEETFVAHI MAFAGCVEPY TDCNLPAPTT 500
    ATSIPDAAHL AASPPPLALL AGAMLLLLAP TLY 533
    Length:533
    Mass (Da):57,100
    Last modified:November 1, 1997 - v2
    Checksum:iD74456F3F1F06714
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti148 – 1503VTS → TSV AA sequence (PubMed:3902089)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07733 Genomic DNA. Translation: AAA30571.1.
    PIRiS30364.
    UniGeneiBt.106.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07733 Genomic DNA. Translation: AAA30571.1 .
    PIRi S30364.
    UniGenei Bt.106.

    3D structure databases

    ProteinModelPortali P19111.
    SMRi P19111. Positions 20-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P19111. 2 interactions.

    Chemistry

    BindingDBi P19111.
    ChEMBLi CHEMBL5695.

    Proteomic databases

    PRIDEi P19111.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi P19111.

    Enzyme and pathway databases

    SABIO-RK P19111.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of the bovine intestinal alkaline phosphatase gene: biochemical characterization of the recombinant enzyme."
      Weissig H., Schildge A., Hoylaerts M.F., Iqbal M., Millan J.L.
      Biochem. J. 290:503-508(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    2. "The active-site and amino-terminal amino acid sequence of bovine intestinal alkaline phosphatase."
      Culp J.S., Hermodson M., Butler L.G.
      Biochim. Biophys. Acta 831:330-334(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-35 AND 91-152, ACTIVE SITE.
      Tissue: Intestine.
    3. "Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
      Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
      Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-63.

    Entry informationi

    Entry nameiPPBI_BOVIN
    AccessioniPrimary (citable) accession number: P19111
    Secondary accession number(s): Q28124
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3