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Protein

Intestinal-type alkaline phosphatase

Gene

ALPI

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61MagnesiumBy similarity1
Metal bindingi61Zinc 1By similarity1
Active sitei111Phosphoserine intermediatePROSITE-ProRule annotation1 Publication1
Metal bindingi111Zinc 1By similarity1
Metal bindingi174MagnesiumBy similarity1
Metal bindingi330MagnesiumBy similarity1
Metal bindingi335Zinc 2By similarity1
Metal bindingi339Zinc 2; via tele nitrogenBy similarity1
Metal bindingi376Zinc 1By similarity1
Metal bindingi377Zinc 1; via tele nitrogenBy similarity1
Metal bindingi451Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 908.
SABIO-RKP19111.

Names & Taxonomyi

Protein namesi
Recommended name:
Intestinal-type alkaline phosphatase (EC:3.1.3.1)
Short name:
IAP
Short name:
Intestinal alkaline phosphatase
Gene namesi
Name:ALPI
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5695.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 192 PublicationsAdd BLAST19
ChainiPRO_000002403520 – 506Intestinal-type alkaline phosphataseAdd BLAST487
PropeptideiPRO_0000024036507 – 533Removed in mature formBy similarityAdd BLAST27

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi140 ↔ 202By similarity
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Glycosylationi429N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi486 ↔ 493By similarity
Lipidationi506GPI-anchor amidated aspartateBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP19111.
PRIDEiP19111.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP19111. 2 interactors.
STRINGi9913.ENSBTAP00000005937.

Chemistry databases

BindingDBiP19111.

Structurei

3D structure databases

ProteinModelPortaliP19111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP19111.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQGACVLLLL GLHLQLSLGL VPVEEEDPAF WNRQAAQALD VAKKLQPIQT
60 70 80 90 100
AAKNVILFLG DGMGVPTVTA TRILKGQMNG KLGPETPLAM DQFPYVALSK
110 120 130 140 150
TYNVDRQVPD SAGTATAYLC GVKGNYRTIG VSAAARYNQC KTTRGNEVTS
160 170 180 190 200
VMNRAKKAGK SVGVVTTTRV QHASPAGAYA HTVNRNWYSD ADLPADAQMN
210 220 230 240 250
GCQDIAAQLV NNMDIDVILG GGRKYMFPVG TPDPEYPDDA SVNGVRKRKQ
260 270 280 290 300
NLVQAWQAKH QGAQYVWNRT ALLQAADDSS VTHLMGLFEP ADMKYNVQQD
310 320 330 340 350
HTKDPTLQEM TEVALRVVSR NPRGFYLFVE GGRIDHGHHD DKAYMALTEA
360 370 380 390 400
GMFDNAIAKA NELTSELDTL ILVTADHSHV FSFGGYTLRG TSIFGLAPSK
410 420 430 440 450
ALDSKSYTSI LYGNGPGYAL GGGSRPDVND STSEDPSYQQ QAAVPQASET
460 470 480 490 500
HGGEDVAVFA RGPQAHLVHG VEEETFVAHI MAFAGCVEPY TDCNLPAPTT
510 520 530
ATSIPDAAHL AASPPPLALL AGAMLLLLAP TLY
Length:533
Mass (Da):57,100
Last modified:November 1, 1997 - v2
Checksum:iD74456F3F1F06714
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148 – 150VTS → TSV AA sequence (PubMed:3902089).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07733 Genomic DNA. Translation: AAA30571.1.
PIRiS30364.
UniGeneiBt.106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07733 Genomic DNA. Translation: AAA30571.1.
PIRiS30364.
UniGeneiBt.106.

3D structure databases

ProteinModelPortaliP19111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP19111. 2 interactors.
STRINGi9913.ENSBTAP00000005937.

Chemistry databases

BindingDBiP19111.
ChEMBLiCHEMBL5695.

Proteomic databases

PaxDbiP19111.
PRIDEiP19111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP19111.

Enzyme and pathway databases

BRENDAi3.1.3.1. 908.
SABIO-RKP19111.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBI_BOVIN
AccessioniPrimary (citable) accession number: P19111
Secondary accession number(s): Q28124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.