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Protein

ATP-dependent RNA helicase p62

Gene

Rm62

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As an RNA helicase, unwinds RNA and alters RNA structures through ATP binding and hydrolysis. Involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, rRNA processing and miRNA processing, as well as transcription regulation (By similarity) (PubMed:12368261). Plays a role in innate immunity. Specifically restricts bunyavirus infection, including Rift Valley fever virus (RVFV) or La Crosse virus (LACV), but not vesicular stomatitis virus (VSV), in an interferon- and DROSHA-independent manner (PubMed:25126784).By similarity2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi325 – 332ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • mRNA binding Source: FlyBase

GO - Biological processi

  • antimicrobial humoral response Source: FlyBase
  • defense response to virus Source: UniProtKB-KW
  • mRNA splicing, via spliceosome Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
  • regulation of defense response to virus Source: FlyBase
  • regulation of translation Source: UniProtKB-KW
  • RNA interference Source: FlyBase
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processAntiviral defense, Immunity, mRNA processing, mRNA splicing, RNA-mediated gene silencing, Translation regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase p62 (EC:3.6.4.13)
Gene namesi
Name:Rm62
Synonyms:Dmp68, p62
ORF Names:CG10279
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003261. Rm62.

Subcellular locationi

  • Nucleus By similarity
  • Nucleusnucleolus By similarity
  • Cytoplasmcytosol By similarity

  • Note: In the course of bunyavirus infection, relocalizes from the nucleus to the cytosol where it binds viral RNA to antagonize replication.By similarity

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • cytoplasm Source: FlyBase
  • cytosol Source: UniProtKB-SubCell
  • nucleolus Source: GO_Central
  • nucleus Source: FlyBase
  • polytene chromosome Source: FlyBase
  • polytene chromosome puff Source: FlyBase
  • precatalytic spliceosome Source: FlyBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No phenotype under normal conditions. Upon infection with Rift Valley fever virus (RVFV) or La Crosse virus (LACV), knockdown flies exhibit increased mortality. No phenotype upon infection with other viruses, including vesicular stomatitis virus (VSV), Sindbis virus (SINV), nor Drosophila C virus (DCV).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550071 – 719ATP-dependent RNA helicase p62Add BLAST719

Proteomic databases

PaxDbiP19109.
PRIDEiP19109.

Expressioni

Gene expression databases

BgeeiFBgn0003261.
ExpressionAtlasiP19109. differential.
GenevisibleiP19109. DM.

Interactioni

Subunit structurei

Interacts with Fmr1 to form a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are mRpL5, mRpL11, AGO2 and Dcr-1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Fmr1Q9NFU04EBI-200734,EBI-422631

Protein-protein interaction databases

BioGridi65943. 24 interactors.
DIPiDIP-17867N.
IntActiP19109. 36 interactors.
STRINGi7227.FBpp0078301.

Structurei

3D structure databases

ProteinModelPortaliP19109.
SMRiP19109.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini312 – 487Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini519 – 664Helicase C-terminalPROSITE-ProRule annotationAdd BLAST146

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi281 – 309Q motifAdd BLAST29
Motifi435 – 438DEAD box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi153 – 225Gly-richAdd BLAST73
Compositional biasi671 – 713Gly-richAdd BLAST43

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0331. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00820000126976.
InParanoidiP19109.
KOiK12823.
OMAiMAPHDRD.
OrthoDBiEOG091G033A.
PhylomeDBiP19109.

Family and domain databases

InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P19109-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLKLVQYIAP RVGGATPRPT ACGWGNLLLI SPRSGASSEK CITQRRHFLF
60 70 80 90 100
SSASSSGTFA SSSSLCTEQR QQFHGSRRNR ETILFPSTYS SLQAQSQRAF
110 120 130 140 150
RDSSKPDSDD YVDSIPKAEQ RTRTRKSLFN DPDERTEEIK IEGVMAPHDR
160 170 180 190 200
DFGHSGRGGR GGDRGGDDRR GGGGGGNRFG GGGGGGDYHG IRNGRVEKRR
210 220 230 240 250
DDRGGGNRFG GGGGFGDRRG GGGGGSQDLP MRPVDFSNLA PFKKNFYQEH
260 270 280 290 300
PNVANRSPYE VQRYREEQEI TVRGQVPNPI QDFSEVHLPD YVMKEIRRQG
310 320 330 340 350
YKAPTAIQAQ GWPIAMSGSN FVGIAKTGSG KTLGYILPAI VHINNQQPLQ
360 370 380 390 400
RGDGPIALVL APTRELAQQI QQVATEFGSS SYVRNTCVFG GAPKGGQMRD
410 420 430 440 450
LQRGCEIVIA TPGRLIDFLS AGSTNLKRCT YLVLDEADRM LDMGFEPQIR
460 470 480 490 500
KIVSQIRPDR QTLMWSATWP KEVKQLAEDF LGNYIQINIG SLELSANHNI
510 520 530 540 550
RQVVDVCDEF SKEEKLKTLL SDIYDTSESP GKIIIFVETK RRVDNLVRFI
560 570 580 590 600
RSFGVRCGAI HGDKSQSERD FVLREFRSGK SNILVATDVA ARGLDVDGIK
610 620 630 640 650
YVINFDYPQN SEDYIHRIGR TGRSNTKGTS FAFFTKNNAK QAKALVDVLR
660 670 680 690 700
EANQEINPAL ENLARNSRYD GGGGRSRYGG GGGGGRFGGG GFKKGSLSNG
710
RGFGGGGGGG GEGRHSRFD
Note: No experimental confirmation available.
Length:719
Mass (Da):78,548
Last modified:May 16, 2003 - v3
Checksum:iA70A071A920B24FC
GO
Isoform C (identifier: P19109-2) [UniParc]FASTAAdd to basket
Also known as: B, F

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.
     142-144: EGV → MMM

Note: No experimental confirmation available.
Show »
Length:578
Mass (Da):62,867
Checksum:i2A948C1A9D7ED561
GO
Isoform D (identifier: P19109-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-144: Missing.

Show »
Length:575
Mass (Da):62,474
Checksum:i77007C1A9D6E51E9
GO
Isoform E (identifier: P19109-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-141: Missing.
     142-145: EGVM → MRAK

Note: No experimental confirmation available.
Show »
Length:578
Mass (Da):62,829
Checksum:i7B4A17CFD243D0E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195R → A in CAA37037 (PubMed:2170937).Curated1
Sequence conflicti675R → P in CAA37037 (PubMed:2170937).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0074141 – 144Missing in isoform D. 2 PublicationsAdd BLAST144
Alternative sequenceiVSP_0074131 – 141Missing in isoform C and isoform E. 1 PublicationAdd BLAST141
Alternative sequenceiVSP_007416142 – 145EGVM → MRAK in isoform E. Curated4
Alternative sequenceiVSP_007415142 – 144EGV → MMM in isoform C. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52846 mRNA. Translation: CAA37037.1.
AE014297 Genomic DNA. Translation: AAF51926.2.
AE014297 Genomic DNA. Translation: AAF51927.2.
AE014297 Genomic DNA. Translation: AAG22212.1.
AE014297 Genomic DNA. Translation: AAG22213.2.
AE014297 Genomic DNA. Translation: AAN14331.1.
AE014297 Genomic DNA. Translation: AAN14332.1.
BT001716 mRNA. Translation: AAN71471.1.
BT011476 mRNA. Translation: AAR99134.1.
BT015209 mRNA. Translation: AAT94438.1.
PIRiS11485.
RefSeqiNP_001163528.1. NM_001170057.1. [P19109-3]
NP_001189182.1. NM_001202253.2. [P19109-4]
NP_524243.2. NM_079519.2. [P19109-1]
NP_731031.1. NM_169118.1. [P19109-3]
NP_731032.1. NM_169119.3. [P19109-4]
NP_731033.1. NM_169120.1. [P19109-2]
NP_731034.1. NM_169121.2. [P19109-2]
NP_731035.2. NM_169122.1. [P19109-2]
UniGeneiDm.1520.

Genome annotation databases

EnsemblMetazoaiFBtr0078649; FBpp0078298; FBgn0003261. [P19109-2]
FBtr0078650; FBpp0078299; FBgn0003261. [P19109-3]
FBtr0078651; FBpp0078300; FBgn0003261. [P19109-2]
FBtr0078652; FBpp0078301; FBgn0003261. [P19109-1]
FBtr0078653; FBpp0078302; FBgn0003261. [P19109-4]
FBtr0078654; FBpp0078303; FBgn0003261. [P19109-2]
FBtr0301947; FBpp0291159; FBgn0003261. [P19109-3]
FBtr0302597; FBpp0291753; FBgn0003261. [P19109-4]
GeneIDi40739.
KEGGidme:Dmel_CG10279.
UCSCiCG10279-RC. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDDX17_DROME
AccessioniPrimary (citable) accession number: P19109
Secondary accession number(s): A4V2H1
, Q6AWN9, Q8IGL7, Q95TB8, Q9I7P5, Q9VNK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 16, 2003
Last modified: August 30, 2017
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families