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P19105 (ML12A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Myosin regulatory light chain 12A
Alternative name(s):
Epididymis secretory protein Li 24
Short name=HEL-S-24
MLC-2B
Myosin RLC
Myosin regulatory light chain 2, nonsarcomeric
Myosin regulatory light chain MRLC3
Gene names
Name:MYL12A
Synonyms:MLCB, MRLC3, RLC
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length171 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion By similarity.

Subunit structure

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Post-translational modification

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge By similarity.

Miscellaneous

This chain binds calcium.

Sequence similarities

Contains 3 EF-hand domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Rock2Q628682EBI-354418,EBI-1569209From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 171171Myosin regulatory light chain 12A
PRO_0000198733

Regions

Domain28 – 6336EF-hand 1
Domain97 – 13236EF-hand 2
Domain133 – 16836EF-hand 3
Calcium binding41 – 5212

Amino acid modifications

Modified residue181Phosphothreonine; by MLCK
Modified residue191Phosphoserine; by MLCK

Sequences

Sequence LengthMass (Da)Tools
P19105 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: C871AA881BF5C215

FASTA17119,794
        10         20         30         40         50         60 
MSSKRTKTKT KKRPQRATSN VFAMFDQSQI QEFKEAFNMI DQNRDGFIDK EDLHDMLASL 

        70         80         90        100        110        120 
GKNPTDEYLD AMMNEAPGPI NFTMFLTMFG EKLNGTDPED VIRNAFACFD EEATGTIQED 

       130        140        150        160        170 
YLRELLTTMG DRFTDEEVDE LYREAPIDKK GNFNYIEFTR ILKHGAKDKD D 

« Hide

References

« Hide 'large scale' references
[1]"Human nonsarcomeric 20,000 Da myosin regulatory light chain cDNA."
Grant J.W., Zhong R.Q., McEwen P., Church S.L.
Nucleic Acids Res. 18:5892-5892(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells."
Iwasaki T., Murata-Hori M., Ishitobi S., Hosoya H.
Cell Struct. Funct. 26:677-683(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT THR-18 AND SER-19.
[3]Li J.Y., Wang H.Y., Liu F.J., Liu J.
Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle, Skin and Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54304 mRNA. Translation: CAA38201.1.
D82059 mRNA. Translation: BAB88919.1.
EU794621 mRNA. Translation: ACJ13675.1.
AK291145 mRNA. Translation: BAF83834.1.
CH471113 Genomic DNA. Translation: EAX01678.1.
CH471113 Genomic DNA. Translation: EAX01680.1.
CH471113 Genomic DNA. Translation: EAX01681.1.
BC016372 mRNA. Translation: AAH16372.1.
BC031972 mRNA. Translation: AAH31972.1.
BC032748 mRNA. Translation: AAH32748.1.
PIRMOHULP. S11493.
RefSeqNP_006462.1. NM_006471.2.
XP_005258133.1. XM_005258076.1.
XP_005258135.1. XM_005258078.1.
UniGeneHs.190086.

3D structure databases

ProteinModelPortalP19105.
SMRP19105. Positions 25-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115871. 38 interactions.
IntActP19105. 19 interactions.
MINTMINT-1159148.
STRING9606.ENSP00000217652.

PTM databases

PhosphoSiteP19105.

Polymorphism databases

DMDM127169.

2D gel databases

OGPP19105.
SWISS-2DPAGEP19105.

Proteomic databases

PaxDbP19105.
PRIDEP19105.

Protocols and materials databases

DNASU10627.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000217652; ENSP00000217652; ENSG00000101608.
ENST00000536605; ENSP00000441231; ENSG00000101608.
ENST00000578611; ENSP00000463614; ENSG00000101608.
ENST00000579226; ENSP00000462171; ENSG00000101608.
GeneID10627.
KEGGhsa:10627.
UCSCuc002klr.3. human.

Organism-specific databases

CTD10627.
GeneCardsGC18P003238.
HGNCHGNC:16701. MYL12A.
HPACAB004503.
HPA045244.
MIM609211. gene.
neXtProtNX_P19105.
PharmGKBPA164723273.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5126.
HOGENOMHOG000233018.
InParanoidP19105.
KOK12757.
OrthoDBEOG7992RX.
PhylomeDBP19105.
TreeFamTF314218.

Gene expression databases

ArrayExpressP19105.
BgeeP19105.
GenevestigatorP19105.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamPF00036. EF-hand_1. 1 hit.
[Graphical view]
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMYL12A. human.
GeneWikiMRCL3.
GenomeRNAi10627.
NextBio40378.
PROP19105.
SOURCESearch...

Entry information

Entry nameML12A_HUMAN
AccessionPrimary (citable) accession number: P19105
Secondary accession number(s): Q53X45
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM