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P19105

- ML12A_HUMAN

UniProt

P19105 - ML12A_HUMAN

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Protein
Myosin regulatory light chain 12A
Gene
MYL12A, MLCB, MRLC3, RLC
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi41 – 5212
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. protein targeting to plasma membrane Source: Ensembl
  2. regulation of cell shape Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 12A
Alternative name(s):
Epididymis secretory protein Li 24
Short name:
HEL-S-24
MLC-2B
Myosin RLC
Myosin regulatory light chain 2, nonsarcomeric
Myosin regulatory light chain MRLC3
Gene namesi
Name:MYL12A
Synonyms:MLCB, MRLC3, RLC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:16701. MYL12A.

Subcellular locationi

GO - Cellular componenti

  1. Z disc Source: Ensembl
  2. extracellular vesicular exosome Source: UniProt
  3. myosin II complex Source: Ensembl
  4. stress fiber Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164723273.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 171171Myosin regulatory light chain 12A
PRO_0000198733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphothreonine; by MLCK1 Publication
Modified residuei19 – 191Phosphoserine; by MLCK1 Publication

Post-translational modificationi

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19105.
PRIDEiP19105.

2D gel databases

OGPiP19105.
SWISS-2DPAGEiP19105.

PTM databases

PhosphoSiteiP19105.

Expressioni

Gene expression databases

ArrayExpressiP19105.
BgeeiP19105.
GenevestigatoriP19105.

Organism-specific databases

HPAiCAB004503.
HPA045244.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
Rock2Q628682EBI-354418,EBI-1569209From a different organism.

Protein-protein interaction databases

BioGridi115871. 38 interactions.
IntActiP19105. 19 interactions.
MINTiMINT-1159148.
STRINGi9606.ENSP00000217652.

Structurei

3D structure databases

ProteinModelPortaliP19105.
SMRiP19105. Positions 25-167.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6336EF-hand 1
Add
BLAST
Domaini97 – 13236EF-hand 2
Add
BLAST
Domaini133 – 16836EF-hand 3
Add
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
HOGENOMiHOG000233018.
InParanoidiP19105.
KOiK12757.
OrthoDBiEOG7992RX.
PhylomeDBiP19105.
TreeFamiTF314218.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19105-1 [UniParc]FASTAAdd to Basket

« Hide

MSSKRTKTKT KKRPQRATSN VFAMFDQSQI QEFKEAFNMI DQNRDGFIDK    50
EDLHDMLASL GKNPTDEYLD AMMNEAPGPI NFTMFLTMFG EKLNGTDPED 100
VIRNAFACFD EEATGTIQED YLRELLTTMG DRFTDEEVDE LYREAPIDKK 150
GNFNYIEFTR ILKHGAKDKD D 171
Length:171
Mass (Da):19,794
Last modified:January 23, 2007 - v2
Checksum:iC871AA881BF5C215
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54304 mRNA. Translation: CAA38201.1.
D82059 mRNA. Translation: BAB88919.1.
EU794621 mRNA. Translation: ACJ13675.1.
AK291145 mRNA. Translation: BAF83834.1.
CH471113 Genomic DNA. Translation: EAX01678.1.
CH471113 Genomic DNA. Translation: EAX01680.1.
CH471113 Genomic DNA. Translation: EAX01681.1.
BC016372 mRNA. Translation: AAH16372.1.
BC031972 mRNA. Translation: AAH31972.1.
BC032748 mRNA. Translation: AAH32748.1.
CCDSiCCDS11830.1.
PIRiS11493. MOHULP.
RefSeqiNP_006462.1. NM_006471.2.
XP_005258133.1. XM_005258076.1.
XP_005258135.1. XM_005258078.1.
UniGeneiHs.190086.

Genome annotation databases

EnsembliENST00000217652; ENSP00000217652; ENSG00000101608.
ENST00000536605; ENSP00000441231; ENSG00000101608.
ENST00000578611; ENSP00000463614; ENSG00000101608.
ENST00000579226; ENSP00000462171; ENSG00000101608.
GeneIDi10627.
KEGGihsa:10627.
UCSCiuc002klr.3. human.

Polymorphism databases

DMDMi127169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54304 mRNA. Translation: CAA38201.1 .
D82059 mRNA. Translation: BAB88919.1 .
EU794621 mRNA. Translation: ACJ13675.1 .
AK291145 mRNA. Translation: BAF83834.1 .
CH471113 Genomic DNA. Translation: EAX01678.1 .
CH471113 Genomic DNA. Translation: EAX01680.1 .
CH471113 Genomic DNA. Translation: EAX01681.1 .
BC016372 mRNA. Translation: AAH16372.1 .
BC031972 mRNA. Translation: AAH31972.1 .
BC032748 mRNA. Translation: AAH32748.1 .
CCDSi CCDS11830.1.
PIRi S11493. MOHULP.
RefSeqi NP_006462.1. NM_006471.2.
XP_005258133.1. XM_005258076.1.
XP_005258135.1. XM_005258078.1.
UniGenei Hs.190086.

3D structure databases

ProteinModelPortali P19105.
SMRi P19105. Positions 25-167.
ModBasei Search...

Protein-protein interaction databases

BioGridi 115871. 38 interactions.
IntActi P19105. 19 interactions.
MINTi MINT-1159148.
STRINGi 9606.ENSP00000217652.

PTM databases

PhosphoSitei P19105.

Polymorphism databases

DMDMi 127169.

2D gel databases

OGPi P19105.
SWISS-2DPAGEi P19105.

Proteomic databases

PaxDbi P19105.
PRIDEi P19105.

Protocols and materials databases

DNASUi 10627.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000217652 ; ENSP00000217652 ; ENSG00000101608 .
ENST00000536605 ; ENSP00000441231 ; ENSG00000101608 .
ENST00000578611 ; ENSP00000463614 ; ENSG00000101608 .
ENST00000579226 ; ENSP00000462171 ; ENSG00000101608 .
GeneIDi 10627.
KEGGi hsa:10627.
UCSCi uc002klr.3. human.

Organism-specific databases

CTDi 10627.
GeneCardsi GC18P003238.
HGNCi HGNC:16701. MYL12A.
HPAi CAB004503.
HPA045244.
MIMi 609211. gene.
neXtProti NX_P19105.
PharmGKBi PA164723273.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5126.
HOGENOMi HOG000233018.
InParanoidi P19105.
KOi K12757.
OrthoDBi EOG7992RX.
PhylomeDBi P19105.
TreeFami TF314218.

Miscellaneous databases

ChiTaRSi MYL12A. human.
GeneWikii MRCL3.
GenomeRNAii 10627.
NextBioi 40378.
PROi P19105.
SOURCEi Search...

Gene expression databases

ArrayExpressi P19105.
Bgeei P19105.
Genevestigatori P19105.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
InterProi IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view ]
Pfami PF00036. EF-hand_1. 1 hit.
[Graphical view ]
SMARTi SM00054. EFh. 2 hits.
[Graphical view ]
PROSITEi PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human nonsarcomeric 20,000 Da myosin regulatory light chain cDNA."
    Grant J.W., Zhong R.Q., McEwen P., Church S.L.
    Nucleic Acids Res. 18:5892-5892(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells."
    Iwasaki T., Murata-Hori M., Ishitobi S., Hosoya H.
    Cell Struct. Funct. 26:677-683(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT THR-18 AND SER-19.
  3. Li J.Y., Wang H.Y., Liu F.J., Liu J.
    Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skeletal muscle, Skin and Testis.

Entry informationi

Entry nameiML12A_HUMAN
AccessioniPrimary (citable) accession number: P19105
Secondary accession number(s): Q53X45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This chain binds calcium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi