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Protein

Myosin regulatory light chain 12A

Gene

MYL12A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).By similarity

Miscellaneous

This chain binds calcium.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi41 – 52Add BLAST12

GO - Molecular functioni

GO - Biological processi

  • ephrin receptor signaling pathway Source: Reactome
  • muscle contraction Source: Reactome
  • platelet aggregation Source: UniProtKB

Keywordsi

Molecular functionMotor protein, Muscle protein, Myosin
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-3928664. Ephrin signaling.
R-HSA-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 12A
Alternative name(s):
Epididymis secretory protein Li 24
Short name:
HEL-S-24
MLC-2B
Myosin RLC
Myosin regulatory light chain 2, nonsarcomeric
Myosin regulatory light chain MRLC3
Gene namesi
Name:MYL12A
Synonyms:MLCB, MRLC3, RLC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 18

Organism-specific databases

EuPathDBiHostDB:ENSG00000101608.12.
HGNCiHGNC:16701. MYL12A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi103910.
10627.
OpenTargetsiENSG00000101608.
PharmGKBiPA164723273.

Polymorphism and mutation databases

BioMutaiMYL12A.
DMDMi127169.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987331 – 171Myosin regulatory light chain 12AAdd BLAST171

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Phosphothreonine; by MLCK1 Publication1
Modified residuei19Phosphoserine; by MLCK1 Publication1

Post-translational modificationi

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP19105.
PaxDbiP19105.
PeptideAtlasiP19105.
PRIDEiP19105.
TopDownProteomicsiP19105.

2D gel databases

OGPiP19105.
SWISS-2DPAGEiP19105.

PTM databases

iPTMnetiP19105.
PhosphoSitePlusiP19105.
SwissPalmiP19105.

Expressioni

Gene expression databases

BgeeiENSG00000101608.
ExpressionAtlasiP19105. baseline and differential.
GenevisibleiP19105. HS.

Organism-specific databases

HPAiCAB004503.
HPA039262.
HPA045244.
HPA059197.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
Rock2Q628682EBI-354418,EBI-1569209From Rattus norvegicus.

Protein-protein interaction databases

BioGridi115871. 52 interactors.
CORUMiP19105.
IntActiP19105. 32 interactors.
MINTiMINT-1159148.
STRINGi9606.ENSP00000217652.

Structurei

3D structure databases

ProteinModelPortaliP19105.
SMRiP19105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 63EF-hand 1Add BLAST36
Domaini97 – 132EF-hand 2Add BLAST36
Domaini133 – 168EF-hand 3Add BLAST36

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0031. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
InParanoidiP19105.
KOiK12757.
PhylomeDBiP19105.
TreeFamiTF314218.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR015070. EF_hand_DJBP.
IPR002048. EF_hand_dom.
PfamiView protein in Pfam
PF08976. EF-hand_11. 1 hit.
PF13499. EF-hand_7. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 2 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.

Sequencei

Sequence statusi: Complete.

P19105-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKRTKTKT KKRPQRATSN VFAMFDQSQI QEFKEAFNMI DQNRDGFIDK
60 70 80 90 100
EDLHDMLASL GKNPTDEYLD AMMNEAPGPI NFTMFLTMFG EKLNGTDPED
110 120 130 140 150
VIRNAFACFD EEATGTIQED YLRELLTTMG DRFTDEEVDE LYREAPIDKK
160 170
GNFNYIEFTR ILKHGAKDKD D
Length:171
Mass (Da):19,794
Last modified:January 23, 2007 - v2
Checksum:iC871AA881BF5C215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54304 mRNA. Translation: CAA38201.1.
D82059 mRNA. Translation: BAB88919.1.
EU794621 mRNA. Translation: ACJ13675.1.
AK291145 mRNA. Translation: BAF83834.1.
CH471113 Genomic DNA. Translation: EAX01678.1.
CH471113 Genomic DNA. Translation: EAX01680.1.
CH471113 Genomic DNA. Translation: EAX01681.1.
BC016372 mRNA. Translation: AAH16372.1.
BC031972 mRNA. Translation: AAH31972.1.
BC032748 mRNA. Translation: AAH32748.1.
CCDSiCCDS11830.1.
PIRiS11493. MOHULP.
RefSeqiNP_001289976.1. NM_001303047.1.
NP_001289977.1. NM_001303048.1.
NP_001289978.1. NM_001303049.1.
NP_006462.1. NM_006471.3.
UniGeneiHs.190086.

Genome annotation databases

EnsembliENST00000217652; ENSP00000217652; ENSG00000101608.
ENST00000536605; ENSP00000441231; ENSG00000101608.
ENST00000578611; ENSP00000463614; ENSG00000101608.
ENST00000579226; ENSP00000462171; ENSG00000101608.
GeneIDi10627.
KEGGihsa:10627.
UCSCiuc002klr.3. human.

Similar proteinsi

Entry informationi

Entry nameiML12A_HUMAN
AccessioniPrimary (citable) accession number: P19105
Secondary accession number(s): Q53X45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot