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Protein

Myosin regulatory light chain 12A

Gene

MYL12A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi41 – 5212Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. platelet aggregation Source: UniProtKB
  2. protein targeting to plasma membrane Source: Ensembl
  3. regulation of cell shape Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_228226. Ephrin signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain 12A
Alternative name(s):
Epididymis secretory protein Li 24
Short name:
HEL-S-24
MLC-2B
Myosin RLC
Myosin regulatory light chain 2, nonsarcomeric
Myosin regulatory light chain MRLC3
Gene namesi
Name:MYL12A
Synonyms:MLCB, MRLC3, RLC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 18

Organism-specific databases

HGNCiHGNC:16701. MYL12A.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProtKB
  2. myosin II complex Source: Ensembl
  3. stress fiber Source: Ensembl
  4. Z disc Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA164723273.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 171171Myosin regulatory light chain 12APRO_0000198733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181Phosphothreonine; by MLCK1 Publication
Modified residuei19 – 191Phosphoserine; by MLCK1 Publication

Post-translational modificationi

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP19105.
PRIDEiP19105.

2D gel databases

OGPiP19105.
SWISS-2DPAGEP19105.

PTM databases

PhosphoSiteiP19105.

Expressioni

Gene expression databases

BgeeiP19105.
ExpressionAtlasiP19105. baseline and differential.
GenevestigatoriP19105.

Organism-specific databases

HPAiCAB004503.
HPA045244.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
Rock2Q628682EBI-354418,EBI-1569209From a different organism.

Protein-protein interaction databases

BioGridi115871. 38 interactions.
IntActiP19105. 20 interactions.
MINTiMINT-1159148.
STRINGi9606.ENSP00000217652.

Structurei

3D structure databases

ProteinModelPortaliP19105.
SMRiP19105. Positions 25-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 6336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 13236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 16836EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
InParanoidiP19105.
KOiK12757.
OrthoDBiEOG7992RX.
PhylomeDBiP19105.
TreeFamiTF314218.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P19105-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSKRTKTKT KKRPQRATSN VFAMFDQSQI QEFKEAFNMI DQNRDGFIDK
60 70 80 90 100
EDLHDMLASL GKNPTDEYLD AMMNEAPGPI NFTMFLTMFG EKLNGTDPED
110 120 130 140 150
VIRNAFACFD EEATGTIQED YLRELLTTMG DRFTDEEVDE LYREAPIDKK
160 170
GNFNYIEFTR ILKHGAKDKD D
Length:171
Mass (Da):19,794
Last modified:January 23, 2007 - v2
Checksum:iC871AA881BF5C215
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54304 mRNA. Translation: CAA38201.1.
D82059 mRNA. Translation: BAB88919.1.
EU794621 mRNA. Translation: ACJ13675.1.
AK291145 mRNA. Translation: BAF83834.1.
CH471113 Genomic DNA. Translation: EAX01678.1.
CH471113 Genomic DNA. Translation: EAX01680.1.
CH471113 Genomic DNA. Translation: EAX01681.1.
BC016372 mRNA. Translation: AAH16372.1.
BC031972 mRNA. Translation: AAH31972.1.
BC032748 mRNA. Translation: AAH32748.1.
CCDSiCCDS11830.1.
PIRiS11493. MOHULP.
RefSeqiNP_006462.1. NM_006471.2.
XP_005258133.1. XM_005258076.1.
XP_005258135.1. XM_005258078.1.
UniGeneiHs.190086.

Genome annotation databases

EnsembliENST00000217652; ENSP00000217652; ENSG00000101608.
ENST00000536605; ENSP00000441231; ENSG00000101608.
ENST00000578611; ENSP00000463614; ENSG00000101608.
ENST00000579226; ENSP00000462171; ENSG00000101608.
GeneIDi10627.
KEGGihsa:10627.
UCSCiuc002klr.3. human.

Polymorphism databases

DMDMi127169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54304 mRNA. Translation: CAA38201.1.
D82059 mRNA. Translation: BAB88919.1.
EU794621 mRNA. Translation: ACJ13675.1.
AK291145 mRNA. Translation: BAF83834.1.
CH471113 Genomic DNA. Translation: EAX01678.1.
CH471113 Genomic DNA. Translation: EAX01680.1.
CH471113 Genomic DNA. Translation: EAX01681.1.
BC016372 mRNA. Translation: AAH16372.1.
BC031972 mRNA. Translation: AAH31972.1.
BC032748 mRNA. Translation: AAH32748.1.
CCDSiCCDS11830.1.
PIRiS11493. MOHULP.
RefSeqiNP_006462.1. NM_006471.2.
XP_005258133.1. XM_005258076.1.
XP_005258135.1. XM_005258078.1.
UniGeneiHs.190086.

3D structure databases

ProteinModelPortaliP19105.
SMRiP19105. Positions 25-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115871. 38 interactions.
IntActiP19105. 20 interactions.
MINTiMINT-1159148.
STRINGi9606.ENSP00000217652.

PTM databases

PhosphoSiteiP19105.

Polymorphism databases

DMDMi127169.

2D gel databases

OGPiP19105.
SWISS-2DPAGEP19105.

Proteomic databases

PaxDbiP19105.
PRIDEiP19105.

Protocols and materials databases

DNASUi10627.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000217652; ENSP00000217652; ENSG00000101608.
ENST00000536605; ENSP00000441231; ENSG00000101608.
ENST00000578611; ENSP00000463614; ENSG00000101608.
ENST00000579226; ENSP00000462171; ENSG00000101608.
GeneIDi10627.
KEGGihsa:10627.
UCSCiuc002klr.3. human.

Organism-specific databases

CTDi10627.
GeneCardsiGC18P003238.
HGNCiHGNC:16701. MYL12A.
HPAiCAB004503.
HPA045244.
MIMi609211. gene.
neXtProtiNX_P19105.
PharmGKBiPA164723273.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00760000119196.
HOGENOMiHOG000233018.
InParanoidiP19105.
KOiK12757.
OrthoDBiEOG7992RX.
PhylomeDBiP19105.
TreeFamiTF314218.

Enzyme and pathway databases

ReactomeiREACT_228226. Ephrin signaling.

Miscellaneous databases

ChiTaRSiMYL12A. human.
GeneWikiiMRCL3.
GenomeRNAii10627.
NextBioi40378.
PROiP19105.
SOURCEiSearch...

Gene expression databases

BgeeiP19105.
ExpressionAtlasiP19105. baseline and differential.
GenevestigatoriP19105.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human nonsarcomeric 20,000 Da myosin regulatory light chain cDNA."
    Grant J.W., Zhong R.Q., McEwen P., Church S.L.
    Nucleic Acids Res. 18:5892-5892(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells."
    Iwasaki T., Murata-Hori M., Ishitobi S., Hosoya H.
    Cell Struct. Funct. 26:677-683(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION AT THR-18 AND SER-19.
  3. Li J.Y., Wang H.Y., Liu F.J., Liu J.
    Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skeletal muscle, Skin and Testis.

Entry informationi

Entry nameiML12A_HUMAN
AccessioniPrimary (citable) accession number: P19105
Secondary accession number(s): Q53X45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This chain binds calcium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.