P19105 (ML12A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin regulatory light chain 12A

Alternative name(s):
MLC-2B
Myosin RLC
Myosin regulatory light chain 2, nonsarcomeric
Myosin regulatory light chain MRLC3

Gene names

Name:	MYL12A
Synonyms:	MLCB, MRLC3, RLC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion By similarity.
Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational modification	Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge By similarity.
Miscellaneous	This chain binds calcium.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Motor protein Muscle protein Myosin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein targeting to plasma membrane Inferred from electronic annotation. Source: Compara regulation of cell shape Inferred from electronic annotation. Source: Compara
Cellular_component	Z disc Inferred from electronic annotation. Source: Compara myosin II complex Inferred from electronic annotation. Source: Compara stress fiber Inferred from electronic annotation. Source: Compara
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Myosin regulatory light chain 12A		PRO_0000198733
Regions
	Domain	28 – 63	36	EF-hand 1
	Domain	97 – 132	36	EF-hand 2
	Domain	133 – 168	36	EF-hand 3
	Calcium binding	41 – 52	12
Amino acid modifications
	Modified residue	18	1	Phosphothreonine; by MLCK

Sequences

	Sequence		Length	Mass (Da)
	P19105 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: C871AA881BF5C215	FASTA	171	19,794
	10 20 30 40 50 60 MSSKRTKTKT KKRPQRATSN VFAMFDQSQI QEFKEAFNMI DQNRDGFIDK EDLHDMLASL 70 80 90 100 110 120 GKNPTDEYLD AMMNEAPGPI NFTMFLTMFG EKLNGTDPED VIRNAFACFD EEATGTIQED 130 140 150 160 170 YLRELLTTMG DRFTDEEVDE LYREAPIDKK GNFNYIEFTR ILKHGAKDKD D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human nonsarcomeric 20,000 Da myosin regulatory light chain cDNA." Grant J.W., Zhong R.Q., McEwen P., Church S.L. Nucleic Acids Res. 18:5892-5892(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle, Skin and Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54304 mRNA. Translation: CAA38201.1. BC016372 mRNA. Translation: AAH16372.1. BC031972 mRNA. Translation: AAH31972.1. BC032748 mRNA. Translation: AAH32748.1.
IPI	IPI00220573.
PIR	MOHULP. S11493.
RefSeq	NP_006462.1. NM_006471.2.
UniGene	Hs.190086.
3D structure databases
ProteinModelPortal	P19105.
ModBase	Search...
Protein-protein interaction databases
IntAct	P19105. 9 interactions.
MINT	MINT-1159148.
STRING	9606.ENSP00000217652.
PTM databases
PhosphoSite	P19105.
Polymorphism databases
DMDM	127169.
2D gel databases
OGP	P19105.
SWISS-2DPAGE	P19105.
Proteomic databases
PaxDb	P19105.
PRIDE	P19105.
Protocols and materials databases
DNASU	10627.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000217652; ENSP00000217652; ENSG00000101608. ENST00000536605; ENSP00000441231; ENSG00000101608. ENST00000578611; ENSP00000463614; ENSG00000101608. ENST00000579226; ENSP00000462171; ENSG00000101608.
GeneID	10627.
KEGG	hsa:10627.
UCSC	uc002klr.3. human.
Organism-specific databases
CTD	10627.
GeneCards	GC18P003238.
HGNC	HGNC:16701. MYL12A.
HPA	CAB004503. HPA045244.
MIM	609211. gene.
neXtProt	NX_P19105.
PharmGKB	PA164723273.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5126.
HOGENOM	HOG000233018.
InParanoid	P19105.
KO	K12757.
OMA	NYVAFTR.
OrthoDB	EOG4FTW1S.
PhylomeDB	P19105.
Gene expression databases
ArrayExpress	P19105.
Bgee	P19105.
Genevestigator	P19105.
GermOnline	ENSG00000101608. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF00036. efhand. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	MYL12A. human.
GenomeRNAi	10627.
NextBio	40378.
SOURCE	Search...

Entry information

Entry name

ML12A_HUMAN

Accession

Primary (citable) accession number: P19105

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.