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P19100 (CP17A_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:CYP17A1
Synonyms:CYP17
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Enzyme regulation

Regulated predominantly by intracellular cAMP levels.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051940

Sites

Metal binding4421Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict46 – 538RGHQHMNF → FL no nucleotide entry Ref.1
Sequence conflict511M → I in CAA77878. Ref.3
Sequence conflict701S → D no nucleotide entry Ref.1
Sequence conflict981R → K no nucleotide entry Ref.1
Sequence conflict108 – 1169Missing in CAA77878. Ref.3
Sequence conflict1171H → E no nucleotide entry Ref.1
Sequence conflict126 – 14015KLALS…KGGNL → SLF no nucleotide entry Ref.1
Sequence conflict161 – 19131NGESI…SFKKG → VIQNACEMDRLKEI no nucleotide entry Ref.1
Sequence conflict1961Q → D no nucleotide entry Ref.1
Sequence conflict201 – 21010FNDGILDAVG → IEEGELT no nucleotide entry Ref.1
Sequence conflict2381M → E no nucleotide entry Ref.1
Sequence conflict257 – 26610NSITNLLDIM → IL no nucleotide entry Ref.1
Sequence conflict270 – 28415Missing no nucleotide entry Ref.1
Sequence conflict291 – 30313HMLAT…IFGAG → AC no nucleotide entry Ref.1
Sequence conflict2921M → S in CAA77878. Ref.3
Sequence conflict3081A → V no nucleotide entry Ref.1
Sequence conflict311 – 33323VKWIV…QDAID → FIWIQEAIE no nucleotide entry Ref.1
Sequence conflict319 – 3213LHY → ATLC in CAA77878. Ref.3
Sequence conflict3301D → E in CAA77878. Ref.3
Sequence conflict3331D → E in CAA77878. Ref.3
Sequence conflict3891D → A no nucleotide entry Ref.1
Sequence conflict395 – 3973NLW → SLF no nucleotide entry Ref.1
Sequence conflict4071H → L in AAA31008. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P19100 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 9497D185A1B446B4

FASTA50957,447
        10         20         30         40         50         60 
MWVLLVFFLL TLTYLFWPKT KGSGAKYPRS LPVLPVVGSL PFLPRRGHQH MNFFKLQDKY 

        70         80         90        100        110        120 
GPIFSFRLGS KTTVVIGDHQ LAKEVLLKKG KEFSGRPRVM TLDILSDNQK GIAFADHGTS 

       130        140        150        160        170        180 
WQLHRKLALS TFSLFKGGNL KLENIINQEI KVLCDFLATR NGESIDLAQP LSLAMTNIVS 

       190        200        210        220        230        240 
FICFNFSFKK GDPALQAIVN FNDGILDAVG KEILYDMFPG IRILPSQTLE NMKQCVRMRN 

       250        260        270        280        290        300 
ELLREILENR KENYSRNSIT NLLDIMIQAK TNAESNTGGP DHNLKLLSDR HMLATVADIF 

       310        320        330        340        350        360 
GAGVETSASV VKWIVAFLLH YPLLRKKIQD AIDQNIGFNR APSISDRNQL VLLEATIREV 

       370        380        390        400        410        420 
LRFRPVSPTL IPHRAIIDSS IGEFTIDKDT DVVVNLWALH HNEKEWHRPD LFMPERFLDP 

       430        440        450        460        470        480 
TGTQLISPSL SYLPFGAGPR SCVGEMLARQ ELFLFTAGLL QRFDLELPDD GQLPCLVGNP 

       490        500 
SLVLQIDPFK VKIKERQAWK EAHTEGSTS 

« Hide

References

[1]"Cytochrome P450c17 (steroid 17 alpha-hydroxylase/17,20 lyase): cloning of human adrenal and testis cDNAs indicates the same gene is expressed in both tissues."
Chung B.-C., Picado-Leonard J., Haniu M., Bienkowski M., Hall P.F., Shively J.E., Miller W.L.
Proc. Natl. Acad. Sci. U.S.A. 84:407-411(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adrenal gland and Testis.
[2]"Nucleotide sequence of a cDNA encoding porcine testis 17 alpha-hydroxylase cytochrome P-450."
Conley A.J., Graham-Lorence S.E., Kagimoto M., Lorence M.C., Murry B.A., Oka K., Sanders D., Mason J.I.
Biochim. Biophys. Acta 1130:75-77(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[3]"Gene for 17 alpha-hydroxylase/C (17-20) lyase P-450: complete nucleotide sequence of the porcine gene and 5' upstream sequence of the rat gene."
Zhang P., Nason T.F., Han X.G., Hall P.F.
Biochim. Biophys. Acta 1131:345-348(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Kidney.
[4]Conley A.J., Chu X., Corbin C.J.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63507 mRNA. Translation: AAA31008.1.
U41525 expand/collapse EMBL AC list , U41519, U41520, U41521, U41522, U41523, U41524 Genomic DNA. Translation: AAA84419.1.
Z11854, Z11855, Z11856 Genomic DNA. Translation: CAA77878.1.
PIRS22339.
RefSeqNP_999593.1. NM_214428.1.
UniGeneSsc.51528.

3D structure databases

ProteinModelPortalP19100.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP19100.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000011585; ENSSSCP00000011283; ENSSSCG00000010591.
GeneID403330.
KEGGssc:403330.

Organism-specific databases

CTD1586.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117317.
HOGENOMHOG000036991.
HOVERGENHBG106944.
KOK00512.
OrthoDBEOG7RBZ85.
TreeFamTF105095.

Enzyme and pathway databases

UniPathwayUPA00062.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP17A_PIG
AccessionPrimary (citable) accession number: P19100
Secondary accession number(s): Q29553, Q99030
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 115 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways