Reviewed, UniProtKB/Swiss-Prot P19088 (DNLI_BPT6)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:

30

Organism

Enterobacteria phage T6 (Bacteriophage T6)

Taxonomic identifier

10666 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase, which is expressed in the early stage of lytic development, has been implicated in T4 DNA synthesis and genetic recombination. It may also play a role in T4 DNA repair.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	DNA ligase		PRO_0000059596
Sites
	Active site	159	1	N6-AMP-lysine intermediate By similarity
	Metal binding	217	1	Divalent metal cation 1 By similarity
	Metal binding	344	1	Divalent metal cation 2 By similarity
	Binding site	164	1	ATP By similarity
	Binding site	182	1	ATP By similarity
	Binding site	217	1	ATP By similarity
	Binding site	359	1	ATP By similarity
	Binding site	365	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19088-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: D560AF09145C6E3B	FASTA	487	55,267
	10 20 30 40 50 60 MILKILNEIA SIGSTKQKQA ILEKNKDNEL LKRVYRLTYS RGLQYYIKKW PKPGIATQSF 70 80 90 100 110 120 GMLTLTDMLD FIEFTLATRK LTGNAAIEEL TGYITDGKKD DVEVLRRVMM RDLECGASVS 130 140 150 160 170 180 IANKVWPGLI PEQPQMLASS YDEKGINKNI KFPAFAQLKA DGARCFAEVR GDELDDVRLL 190 200 210 220 230 240 SRAGNEYLGL DLLKEELIKM TAEARQIHPE GVLIDGELVY HEQVKKEPEG LDFLFDAHPE 250 260 270 280 290 300 NSKVKDFTEV AESRTASNGI ANKSLKGTIS EKEAQCMKFQ VWDYVPLVEV YGLPAFRLKY 310 320 330 340 350 360 DVRFSKLEQM TSGYDKVILI ENQVVNNLDE AKVIYKKYID QGLEGIILKN IDGLWENARS 370 380 390 400 410 420 KNLYKFKEVI DVDLKIVGIY PHRKDPTKAG GFILESECGK IKVNAGSGLK DKAGVKSHEL 430 440 450 460 470 480 DRTRIMENQN YYIGKILECE CNGWLKSDGR TDYVKLFLPI AIRLREDKTK ANTFEDVFGD FHEVTGL

References

[1]

"Comparative analysis of DNA-ligase genes of phages T6 and T4." Kaliman A.V., Zimin A.A., Nazipova N.N., Kryukov V.M., Tanyashin V.I., Kraev A.S., Mironova M.V., Skyrabin K.G., Baev A.A. Dokl. Akad. Nauk SSSR 299:737-742(1988) [PubMed: 3402337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M38465 Genomic DNA. Translation: AAA32562.1.
PIR	S06464.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.5.1.1. 191991.
Family and domain databases
InterPro	IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_BPT6

Accession

Primary (citable) accession number: P19088

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)