P19086 (GNAZ_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(z) subunit alpha

Alternative name(s):
G(x) alpha chain
Gz-alpha

Gene names

Name:

GNAZ

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Subcellular location	Membrane; Lipid-anchor.
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
Cellular component	Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	ADP-ribosylation Lipoprotein Myristate Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara endoplasmic reticulum Traceable author statement PubMed 2117645. Source: ProtInc heterotrimeric G-protein complex Inferred from Biological aspect of Ancestor. Source: RefGenome nuclear envelope Traceable author statement PubMed 2117645. Source: ProtInc
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from Biological aspect of Ancestor. Source: RefGenome G-protein coupled serotonin receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein activity Traceable author statement PubMed 2456569. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 355	354	Guanine nucleotide-binding protein G(z) subunit alpha		PRO_0000203696
Regions
	Nucleotide binding	40 – 47	8	GTP By similarity
	Nucleotide binding	176 – 182	7	GTP By similarity
	Nucleotide binding	201 – 205	5	GTP By similarity
	Nucleotide binding	270 – 273	4	GTP By similarity
Sites
	Metal binding	47	1	Magnesium By similarity
	Metal binding	182	1	Magnesium By similarity
	Binding site	327	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	179	1	ADP-ribosylarginine; by cholera toxin By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity
Experimental info
	Sequence conflict	132	1	W → Y Ref.3
	Sequence conflict	230	1	G → A in BAA14180. Ref.2
	Sequence conflict	273	1	D → N Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P19086 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E868FAE7CD2873EB	FASTA	355	40,924
	10 20 30 40 50 60 MGCRQSSEEK EAARRSRRID RHLRSESQRQ RREIKLLLLG TSNSGKSTIV KQMKIIHSGG 70 80 90 100 110 120 FNLEACKEYK PLIIYNAIDS LTRIIRALAA LRIDFHNPDR AYDAVQLFAL TGPAESKGEI 130 140 150 160 170 180 TPELLGVMRR LWADPGAQAC FSRSSEYHLE DNAAYYLNDL ERIAAADYIP TVEDILRSRD 190 200 210 220 230 240 MTTGIVENKF TFKELTFKMV DVGGQRSERK KWIHCFEGVT AIIFCVELSG YDLKLYEDNQ 250 260 270 280 290 300 TSRMAESLRL FDSICNNNWF INTSLILFLN KKDLLAEKIR RIPLTICFPE YKGQNTYEEA 310 320 330 340 350 AVYIQRQFED LNRNKETKEI YSHFTCATDT SNIQFVFDAV TDVIIQNNLK YIGLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a GTP-binding protein alpha subunit that lacks an apparent ADP-ribosylation site for pertussis toxin." Fong H.K.W., Yoshimoto K.K., Eversole-Cire P., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 85:3066-3070(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of the human gene for Gx alpha, a pertussis toxin-insensitive regulatory GTP-binding protein." Matsuoka M., Itoh H., Kaziro Y. J. Biol. Chem. 265:13215-13220(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification of Gz alpha as a pertussis toxin-insensitive G protein in human platelets and megakaryocytes." Gagnon A.W., Manning D.R., Catani L., Gewirtz A., Poncz M., Brass L.F. Blood 78:1247-1253(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03260 mRNA. Translation: AAA52580.1. D90150 Genomic DNA. Translation: BAA14180.1. AF493899 mRNA. Translation: AAM12613.1. CR456495 mRNA. Translation: CAG30381.1. AK312519 mRNA. Translation: BAG35418.1. CH471095 Genomic DNA. Translation: EAW59559.1. BC078163 mRNA. Translation: AAH78163.1. BC096828 mRNA. Translation: AAH96828.1.
IPI	IPI00328128.
PIR	RGHUGX. A36628.
RefSeq	NP_002064.1. NM_002073.2.
UniGene	Hs.584760.
3D structure databases
ProteinModelPortal	P19086.
SMR	P19086. Positions 6-349.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-139942.
STRING	9606.ENSP00000248996.
PTM databases
PhosphoSite	P19086.
Polymorphism databases
DMDM	121005.
Proteomic databases
PaxDb	P19086.
PRIDE	P19086.
Protocols and materials databases
DNASU	2781.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000248996; ENSP00000248996; ENSG00000128266.
GeneID	2781.
KEGG	hsa:2781.
UCSC	uc002zwu.1. human.
Organism-specific databases
CTD	2781.
GeneCards	GC22P023413.
HGNC	HGNC:4395. GNAZ.
HPA	HPA003011.
MIM	139160. gene.
neXtProt	NX_P19086.
PharmGKB	PA28775.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG282619.
HOGENOM	HOG000038730.
HOVERGEN	HBG063184.
InParanoid	P19086.
KO	K04535.
OMA	IPLTVCF.
OrthoDB	EOG4T4CVQ.
PhylomeDB	P19086.
Enzyme and pathway databases
Pathway_Interaction_DB	endothelinpathway. Endothelins. hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins. lysophospholipid_pathway. LPA receptor mediated events. er_nongenomic_pathway. Plasma membrane estrogen receptor signaling. s1p_s1p1_pathway. S1P1 pathway. s1p_s1p2_pathway. S1P2 pathway. s1p_s1p3_pathway. S1P3 pathway. s1p_s1p4_pathway. S1P4 pathway. s1p_s1p5_pathway. S1P5 pathway. s1p_meta_pathway. Sphingosine 1-phosphate (S1P) pathway.
Reactome	REACT_111102. Signal Transduction.
SignaLink	P19086.
Gene expression databases
Bgee	P19086.
CleanEx	HS_GNAZ.
Genevestigator	P19086.
GermOnline	ENSG00000128266. Homo sapiens.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR001408. Gprotein_alpha_I. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. IPR027417. P-loop_NTPase. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00441. GPROTEINAI.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	2781.
NextBio	10955.
SOURCE	Search...

Entry information

Entry name

GNAZ_HUMAN

Accession

Primary (citable) accession number: P19086
Secondary accession number(s): B2R6C1, Q4QRJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 140 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.