Reviewed, UniProtKB/Swiss-Prot P19086 (GNAZ_HUMAN)

Names and origin

Protein names

Recommended name:
    Guanine nucleotide-binding protein G(z) subunit alpha
Alternative name(s):
    G(x) alpha chain
    Gz-alpha

Gene names

Name:

GNAZ

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Subunit structure	G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Subcellular location	Membrane; Lipid-anchor.
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
Cellular component	Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Transducer
PTM	ADP-ribosylation Lipoprotein Myristate Palmitate
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Ref.1 Traceable author statement. Source: ProtInc
Cellular component	endoplasmic reticulum Traceable author statement. Source: ProtInc nuclear envelope Traceable author statement. Source: ProtInc plasma membrane Ref.1 Inferred from direct assay. Source: HPA
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: ProtInc receptor signaling protein activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Probable
	Chain	2 – 355	354	Guanine nucleotide-binding protein G(z) subunit alpha		PRO_0000203696
Regions
	Nucleotide binding	40 – 47	8	GTP By similarity
	Nucleotide binding	201 – 205	5	GTP By similarity
	Nucleotide binding	270 – 273	4	GTP By similarity
Amino acid modifications
	Modified residue	179	1	ADP-ribosylarginine; by cholera toxin By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity
Experimental info
	Sequence conflict	132	1	W → Y Ref.3
	Sequence conflict	230	1	G → A in BAA14180. Ref.2
	Sequence conflict	273	1	D → N Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P19086-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E868FAE7CD2873EB	FASTA	355	40,924
	10 20 30 40 50 60 MGCRQSSEEK EAARRSRRID RHLRSESQRQ RREIKLLLLG TSNSGKSTIV KQMKIIHSGG 70 80 90 100 110 120 FNLEACKEYK PLIIYNAIDS LTRIIRALAA LRIDFHNPDR AYDAVQLFAL TGPAESKGEI 130 140 150 160 170 180 TPELLGVMRR LWADPGAQAC FSRSSEYHLE DNAAYYLNDL ERIAAADYIP TVEDILRSRD 190 200 210 220 230 240 MTTGIVENKF TFKELTFKMV DVGGQRSERK KWIHCFEGVT AIIFCVELSG YDLKLYEDNQ 250 260 270 280 290 300 TSRMAESLRL FDSICNNNWF INTSLILFLN KKDLLAEKIR RIPLTICFPE YKGQNTYEEA 310 320 330 340 350 AVYIQRQFED LNRNKETKEI YSHFTCATDT SNIQFVFDAV TDVIIQNNLK YIGLC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a GTP-binding protein alpha subunit that lacks an apparent ADP-ribosylation site for pertussis toxin." Fong H.K.W., Yoshimoto K.K., Eversole-Cire P., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 85:3066-3070(1988) [PubMed: 3129724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of the human gene for Gx alpha, a pertussis toxin-insensitive regulatory GTP-binding protein." Matsuoka M., Itoh H., Kaziro Y. J. Biol. Chem. 265:13215-13220(1990) [PubMed: 2115889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification of Gz alpha as a pertussis toxin-insensitive G protein in human platelets and megakaryocytes." Gagnon A.W., Manning D.R., Catani L., Gewirtz A., Poncz M., Brass L.F. Blood 78:1247-1253(1991) [PubMed: 1908722] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: PNS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03260 mRNA. Translation: AAA52580.1. D90150 Genomic DNA. Translation: BAA14180.1. AF493899 mRNA. Translation: AAM12613.1. CR456495 mRNA. Translation: CAG30381.1. BC078163 mRNA. Translation: AAH78163.1. BC096828 mRNA. Translation: AAH96828.1.
IPI	IPI00328128.
PIR	RGHUGX. A36628.
RefSeq	NP_002064.1.
UniGene	Hs.584760
3D structure databases
HSSP	HSSP built from PDB template 1AS3 based on UniProtKB P10824.
ModBase	Search...
PTM databases
PhosphoSite	P19086.
Proteomic databases
PRIDE	P19086.
Genome annotation databases
Ensembl	ENSG00000128266. Homo sapiens. [Contig view]
GeneID	2781.
KEGG	hsa:2781.
Organism-specific databases
GeneCards	GC22P021728.
H-InvDB	HIX0027841.
HGNC	HGNC:4395. GNAZ.
HPA	HPA003011.
MIM	139160. gene.
PharmGKB	PA28775.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P19086.
HOVERGEN	P19086.
OMA	P19086. IPLTVCF.
Enzyme and pathway databases
Pathway_Interaction_DB	endothelinpathway. Endothelins. hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins. lysophospholipid_pathway. LPA receptor mediated events. er_nongenomic_pathway. Plasma membrane estrogen receptor signaling. s1p_s1p1_pathway. S1P1 pathway. s1p_s1p2_pathway. S1P2 pathway. s1p_s1p3_pathway. S1P3 pathway. s1p_s1p4_pathway. S1P4 pathway. s1p_s1p5_pathway. S1P5 pathway. s1p_meta_pathway. Sphingosine 1-phosphate (S1P) pathway.
Gene expression databases
ArrayExpress	P19086.
Bgee	P19086.
CleanEx	HS_GNAZ.
GermOnline	ENSG00000128266. Homo sapiens.
Family and domain databases
InterPro	IPR001019. Gprotein_alph_bd. IPR001408. Gprotein_alpha_I. [Graphical view]
PANTHER	PTHR10218. Gprotein_alph_bd. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00441. GPROTEINAI.
ProDom	PD000281. Gprotein_alpha. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other Resources
NextBio	10955.
SOURCE	Search...

Entry information

Entry name

GNAZ_HUMAN

Accession

Primary (citable) accession number: P19086
Secondary accession number(s): Q4QRJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)