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Protein

Chorismate mutase AroH

Gene

aroH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.1 Publication

Miscellaneous

This enzyme is monofunctional, and its activity is unaffected by the end-product aromatic amino acids.

Catalytic activityi

Chorismate = prephenate.

Kineticsi

  1. KM=67 µM for chorismate (at pH 7.5 and at 30 degrees Celsius)1 Publication
  2. KM=87 µM for chorismate (at pH 7.5 and at 30 degrees Celsius)1 Publication
  3. KM=100 µM for chorismate (at pH 7.5 and at 30 degrees Celsius)1 Publication

    Pathwayi: prephenate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate.
    Proteins known to be involved in this subpathway in this organism are:
    1. Chorismate mutase AroH (aroH), Chorismate mutase AroH (aroH), Protein AroA(G) (aroA)
    This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei7Substrate1
    Binding sitei90Substrate1

    GO - Molecular functioni

    • chorismate mutase activity Source: UniProtKB

    GO - Biological processi

    • aromatic amino acid family biosynthetic process Source: UniProtKB-KW
    • chorismate metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionIsomerase
    Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis

    Enzyme and pathway databases

    BioCyciBSUB:BSU22690-MONOMER
    BRENDAi5.4.99.5 658
    SABIO-RKiP19080
    UniPathwayiUPA00120; UER00203

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chorismate mutase AroH (EC:5.4.99.5)
    Short name:
    CM
    Gene namesi
    Name:aroH
    Ordered Locus Names:BSU22690
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi78E → A: No chorismate mutase activity. 1 Publication1
    Mutagenesisi90R → A: No chorismate mutase activity. 1 Publication1
    Mutagenesisi90R → G: 2-fold decrease in affinity and very low decrease in catalytic efficiency. 1 Publication1
    Mutagenesisi90R → K: Low decrease in catalytic efficiency and in affinity. 1 Publication1

    Chemistry databases

    DrugBankiDB02153 3-Sulfinoalanine
    DB08427 PREPHENIC ACID

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001192031 – 127Chorismate mutase AroHAdd BLAST127

    Proteomic databases

    PaxDbiP19080
    PRIDEiP19080

    Interactioni

    Subunit structurei

    Homotrimer.3 Publications

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100012466

    Structurei

    Secondary structure

    1127
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 11Combined sources10
    Beta strandi13 – 15Combined sources3
    Helixi17 – 35Combined sources19
    Helixi39 – 41Combined sources3
    Beta strandi42 – 49Combined sources8
    Helixi59 – 63Combined sources5
    Beta strandi65 – 67Combined sources3
    Beta strandi73 – 77Combined sources5
    Beta strandi86 – 97Combined sources12
    Helixi101 – 103Combined sources3
    Helixi110 – 115Combined sources6
    Helixi121 – 124Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1COMX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L1-127[»]
    1DBFX-ray1.30A/B/C1-127[»]
    1FNJX-ray1.90A1-127[»]
    1FNKX-ray2.00A1-127[»]
    2CHSX-ray1.90A/B/C/D/E/F/G/H/I/J/K/L1-127[»]
    2CHTX-ray2.20A/B/C/D/E/F/G/H/I/J/K/L1-127[»]
    3ZO8X-ray1.59A/B/C/D/E/F1-127[»]
    3ZOPX-ray1.61A/B/C/D/E/F1-127[»]
    3ZP4X-ray1.80A/B/C/D/E/F1-127[»]
    3ZP7X-ray1.70A/B/C/D/E/F1-127[»]
    ProteinModelPortaliP19080
    SMRiP19080
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP19080

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini3 – 121Chorismate mutase aroH-typePROSITE-ProRule annotationAdd BLAST119

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni47 – 78Substrate bindingBy similarityAdd BLAST32

    Phylogenomic databases

    eggNOGiENOG4105KHN Bacteria
    COG4401 LUCA
    HOGENOMiHOG000043606
    InParanoidiP19080
    KOiK06208
    OMAiCIRVMMT
    PhylomeDBiP19080

    Family and domain databases

    CDDicd02185 AroH, 1 hit
    Gene3Di3.30.1330.40, 1 hit
    InterProiView protein in InterPro
    IPR008243 Chorismate_mutase_AroH
    IPR035959 RutC-like_sf
    PANTHERiPTHR21164 PTHR21164, 1 hit
    PfamiView protein in Pfam
    PF07736 CM_1, 1 hit
    PIRSFiPIRSF005965 Chor_mut_AroH, 1 hit
    ProDomiView protein in ProDom or Entries sharing at least one domain
    PD031888 Chorismate_mutase_AroH, 1 hit
    SUPFAMiSSF55298 SSF55298, 1 hit
    TIGRFAMsiTIGR01796 CM_mono_aroH, 1 hit
    PROSITEiView protein in PROSITE
    PS51167 CHORISMATE_MUT_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P19080-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMIRGIRGAT TVERDTEEEI LQKTKQLLEK IIEENHTKPE DVVQMLLSAT
    60 70 80 90 100
    PDLHAVFPAK AVRELSGWQY VPVTCMQEMD VTGGLKKCIR VMMTVQTDVP
    110 120
    QDQIRHVYLE KVVVLRPDLS LTKNTEL
    Length:127
    Mass (Da):14,517
    Last modified:October 19, 2002 - v2
    Checksum:i5A803614FE1F402E
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti112V → A in AAA22249 (PubMed:2105742).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32278 Genomic DNA Translation: AAA22249.1
    M80245 Genomic DNA Translation: AAA20861.1
    AL009126 Genomic DNA Translation: CAB14185.1
    PIRiA33894
    RefSeqiNP_390150.1, NC_000964.3
    WP_009967606.1, NZ_JNCM01000036.1

    Genome annotation databases

    EnsemblBacteriaiCAB14185; CAB14185; BSU22690
    GeneIDi939005
    KEGGibsu:BSU22690
    PATRICifig|224308.179.peg.2473

    Similar proteinsi

    Entry informationi

    Entry nameiAROH_BACSU
    AccessioniPrimary (citable) accession number: P19080
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: October 19, 2002
    Last modified: March 28, 2018
    This is version 142 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

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