ID   TSN8_HUMAN              Reviewed;         237 AA.
AC   P19075; B2R7T7; Q9BS78;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   24-JAN-2024, entry version 185.
DE   RecName: Full=Tetraspanin-8;
DE            Short=Tspan-8;
DE   AltName: Full=Transmembrane 4 superfamily member 3;
DE   AltName: Full=Tumor-associated antigen CO-029;
GN   Name=TSPAN8; Synonyms=TM4SF3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Colon carcinoma;
RX   PubMed=2395876; DOI=10.1073/pnas.87.17.6833;
RA   Szala S., Kasai Y., Steplewski Z., Rodeck U., Koprowski H.,
RA   Linnenbach A.J.;
RT   "Molecular cloning of cDNA for the human tumor-associated antigen CO-029
RT   and identification of related transmembrane antigens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:6833-6837(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Colon;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ILE-35 AND ALA-73.
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-118.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of multiple
RT   enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
CC   -!- INTERACTION:
CC       P19075; Q16820: MEP1B; NbExp=4; IntAct=EBI-4289938, EBI-968418;
CC       P19075; P54315: PNLIPRP1; NbExp=3; IntAct=EBI-4289938, EBI-8652812;
CC       P19075; Q9NVC3: SLC38A7; NbExp=3; IntAct=EBI-4289938, EBI-10314552;
CC       P19075; P32856-2: STX2; NbExp=3; IntAct=EBI-4289938, EBI-11956649;
CC       P19075; O14925: TIMM23; NbExp=3; IntAct=EBI-4289938, EBI-1047996;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Gastric, colon, rectal, and pancreatic carcinomas.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC       Haematology;
CC       URL="https://atlasgeneticsoncology.org/gene/42585/TSPAN8";
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DR   EMBL; M35252; AAA35709.1; -; mRNA.
DR   EMBL; AK313112; BAG35934.1; -; mRNA.
DR   EMBL; CH471054; EAW97257.1; -; Genomic_DNA.
DR   EMBL; BC005246; AAH05246.1; -; mRNA.
DR   CCDS; CCDS8999.1; -.
DR   PIR; A36056; A36056.
DR   RefSeq; NP_004607.1; NM_004616.2.
DR   RefSeq; XP_006719646.1; XM_006719583.3.
DR   RefSeq; XP_016875402.1; XM_017019913.1.
DR   AlphaFoldDB; P19075; -.
DR   SMR; P19075; -.
DR   BioGRID; 112958; 81.
DR   IntAct; P19075; 6.
DR   STRING; 9606.ENSP00000377003; -.
DR   GlyCosmos; P19075; 1 site, No reported glycans.
DR   GlyGen; P19075; 1 site.
DR   iPTMnet; P19075; -.
DR   PhosphoSitePlus; P19075; -.
DR   SwissPalm; P19075; -.
DR   BioMuta; TSPAN8; -.
DR   DMDM; 116589; -.
DR   jPOST; P19075; -.
DR   MassIVE; P19075; -.
DR   MaxQB; P19075; -.
DR   PaxDb; 9606-ENSP00000377003; -.
DR   PeptideAtlas; P19075; -.
DR   ProteomicsDB; 53629; -.
DR   Pumba; P19075; -.
DR   Antibodypedia; 29509; 361 antibodies from 37 providers.
DR   DNASU; 7103; -.
DR   Ensembl; ENST00000247829.8; ENSP00000247829.3; ENSG00000127324.9.
DR   Ensembl; ENST00000393330.6; ENSP00000377003.2; ENSG00000127324.9.
DR   Ensembl; ENST00000546561.2; ENSP00000447160.1; ENSG00000127324.9.
DR   GeneID; 7103; -.
DR   KEGG; hsa:7103; -.
DR   MANE-Select; ENST00000247829.8; ENSP00000247829.3; NM_004616.3; NP_004607.1.
DR   UCSC; uc001swj.2; human.
DR   AGR; HGNC:11855; -.
DR   CTD; 7103; -.
DR   DisGeNET; 7103; -.
DR   GeneCards; TSPAN8; -.
DR   HGNC; HGNC:11855; TSPAN8.
DR   HPA; ENSG00000127324; Tissue enhanced (intestine, stomach).
DR   MIM; 600769; gene.
DR   neXtProt; NX_P19075; -.
DR   OpenTargets; ENSG00000127324; -.
DR   PharmGKB; PA36556; -.
DR   VEuPathDB; HostDB:ENSG00000127324; -.
DR   eggNOG; KOG3882; Eukaryota.
DR   GeneTree; ENSGT00940000158153; -.
DR   HOGENOM; CLU_055524_4_2_1; -.
DR   InParanoid; P19075; -.
DR   OMA; IAIWIRV; -.
DR   OrthoDB; 5353560at2759; -.
DR   PhylomeDB; P19075; -.
DR   TreeFam; TF352895; -.
DR   PathwayCommons; P19075; -.
DR   SignaLink; P19075; -.
DR   BioGRID-ORCS; 7103; 7 hits in 1085 CRISPR screens.
DR   ChiTaRS; TSPAN8; human.
DR   GeneWiki; TSPAN8; -.
DR   GenomeRNAi; 7103; -.
DR   Pharos; P19075; Tbio.
DR   PRO; PR:P19075; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; P19075; Protein.
DR   Bgee; ENSG00000127324; Expressed in colonic mucosa and 178 other cell types or tissues.
DR   GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005178; F:integrin binding; IEA:Ensembl.
DR   GO; GO:0030195; P:negative regulation of blood coagulation; IEA:Ensembl.
DR   GO; GO:0010468; P:regulation of gene expression; IEA:Ensembl.
DR   GO; GO:0007283; P:spermatogenesis; IEA:Ensembl.
DR   CDD; cd03154; TM4SF3_like_LEL; 1.
DR   Gene3D; 1.10.1450.10; Tetraspanin; 1.
DR   InterPro; IPR018499; Tetraspanin/Peripherin.
DR   InterPro; IPR000301; Tetraspanin_animals.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2_sf.
DR   PANTHER; PTHR19282; TETRASPANIN; 1.
DR   PANTHER; PTHR19282:SF380; TETRASPANIN-8; 1.
DR   Pfam; PF00335; Tetraspanin; 1.
DR   PIRSF; PIRSF002419; Tetraspanin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   PROSITE; PS00421; TM4_1; 1.
DR   Genevisible; P19075; HS.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..237
FT                   /note="Tetraspanin-8"
FT                   /id="PRO_0000219252"
FT   TOPO_DOM        1..9
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        10..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        34..57
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        73..83
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..205
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..237
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        118
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:19159218"
FT   VARIANT         35
FT                   /note="V -> I (in dbSNP:rs17849952)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_061848"
FT   VARIANT         73
FT                   /note="G -> A (in dbSNP:rs3763978)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_020093"
FT   VARIANT         213
FT                   /note="S -> A (in dbSNP:rs1051334)"
FT                   /id="VAR_012054"
SQ   SEQUENCE   237 AA;  26044 MW;  D1058F054EE8C14E CRC64;
     MAGVSACIKY SMFTFNFLFW LCGILILALA IWVRVSNDSQ AIFGSEDVGS SSYVAVDILI
     AVGAIIMILG FLGCCGAIKE SRCMLLLFFI GLLLILLLQV ATGILGAVFK SKSDRIVNET
     LYENTKLLSA TGESEKQFQE AIIVFQEEFK CCGLVNGAAD WGNNFQHYPE LCACLDKQRP
     CQSYNGKQVY KETCISFIKD FLAKNLIIVI GISFGLAVIE ILGLVFSMVL YCQIGNK
//