P19075 (TSN8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraspanin-8
Short name=Tspan-8

Alternative name(s):
Transmembrane 4 superfamily member 3
Tumor-associated antigen CO-029

Gene names

Name:	TSPAN8
Synonyms:	TM4SF3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Gastric, colon, rectal, and pancreatic carcinomas.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of blood coagulation Inferred from electronic annotation. Source: Compara protein glycosylation Traceable author statement Ref.1. Source: ProtInc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosome Traceable author statement PubMed 8661157. Source: ProtInc
Molecular_function	signal transducer activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Tetraspanin-8		PRO_0000219252
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 33	24	Helical; Potential
	Topological domain	34 – 57	24	Extracellular Potential
	Transmembrane	58 – 72	15	Helical; Potential
	Topological domain	73 – 83	11	Cytoplasmic Potential
	Transmembrane	84 – 109	26	Helical; Potential
	Topological domain	110 – 205	96	Extracellular Potential
	Transmembrane	206 – 230	25	Helical; Potential
	Topological domain	231 – 237	7	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Ref.5
Natural variations
	Natural variant	35	1	V → I. Ref.4 Corresponds to variant rs17849952 [ dbSNP | Ensembl ].		VAR_061848
	Natural variant	73	1	G → A. Ref.4 Corresponds to variant rs3763978 [ dbSNP | Ensembl ].		VAR_020093
	Natural variant	213	1	S → A. Corresponds to variant rs1051334 [ dbSNP | Ensembl ].		VAR_012054

Sequences

	Sequence		Length	Mass (Da)
	P19075 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: D1058F054EE8C14E	FASTA	237	26,044
	10 20 30 40 50 60 MAGVSACIKY SMFTFNFLFW LCGILILALA IWVRVSNDSQ AIFGSEDVGS SSYVAVDILI 70 80 90 100 110 120 AVGAIIMILG FLGCCGAIKE SRCMLLLFFI GLLLILLLQV ATGILGAVFK SKSDRIVNET 130 140 150 160 170 180 LYENTKLLSA TGESEKQFQE AIIVFQEEFK CCGLVNGAAD WGNNFQHYPE LCACLDKQRP 190 200 210 220 230 CQSYNGKQVY KETCISFIKD FLAKNLIIVI GISFGLAVIE ILGLVFSMVL YCQIGNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens." Szala S., Kasai Y., Steplewski Z., Rodeck U., Koprowski H., Linnenbach A.J. Proc. Natl. Acad. Sci. U.S.A. 87:6833-6837(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Colon carcinoma.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS ILE-35 AND ALA-73. Tissue: Liver.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-118, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35252 mRNA. Translation: AAA35709.1. AK313112 mRNA. Translation: BAG35934.1. CH471054 Genomic DNA. Translation: EAW97257.1. BC005246 mRNA. Translation: AAH05246.1.
IPI	IPI00015872.
PIR	A36056.
RefSeq	NP_004607.1. NM_004616.2.
UniGene	Hs.170563.
3D structure databases
ProteinModelPortal	P19075.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000247829.
PTM databases
PhosphoSite	P19075.
Polymorphism databases
DMDM	116589.
Proteomic databases
PaxDb	P19075.
PRIDE	P19075.
Protocols and materials databases
DNASU	7103.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000247829; ENSP00000247829; ENSG00000127324. ENST00000393330; ENSP00000377003; ENSG00000127324. ENST00000546561; ENSP00000447160; ENSG00000127324.
GeneID	7103.
KEGG	hsa:7103.
UCSC	uc001swj.1. human.
Organism-specific databases
CTD	7103.
GeneCards	GC12M071518.
HGNC	HGNC:11855. TSPAN8.
HPA	CAB026009.
MIM	600769. gene.
neXtProt	NX_P19075.
PharmGKB	PA36556.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG312327.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	P19075.
OMA	LYCQIGN.
OrthoDB	EOG4WDDD2.
PhylomeDB	P19075.
Gene expression databases
ArrayExpress	P19075.
Bgee	P19075.
CleanEx	HS_TSPAN8.
Genevestigator	P19075.
GermOnline	ENSG00000127324. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7103.
NextBio	27789.
SOURCE	Search...

Entry information

Entry name

TSN8_HUMAN

Accession

Primary (citable) accession number: P19075
Secondary accession number(s): B2R7T7, Q9BS78

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.