Reviewed, UniProtKB/Swiss-Prot P19073 (CDC42_YEAST)

Names and origin

Protein names

Recommended name:
    Cell division control protein 42
Alternative name(s):
    Suppressor of RHO3 protein 2

Gene names

Name:	CDC42
Synonyms:	SRO2
Ordered Locus Names:	YLR229C
ORF Names:	L8083.13

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in development of cell polarity during the cell division cycle, and essential for bud emergence. Affects signaling in the pheromone-response pathway through the STE20 protein kinase. Negatively regulated by the GTPase-activating proteins RGA1, BEM3, and BEM4.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Pheromone response
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Traceable author statement. Source: SGD budding cell apical bud growth Inferred from mutant phenotype. Source: SGD budding cell isotropic bud growth Inferred from mutant phenotype. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW establishment of cell polarity Traceable author statement. Source: SGD exocytosis Inferred from genetic interaction. Source: SGD invasive growth in response to glucose limitation Inferred from mutant phenotype. Source: SGD pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from genetic interaction. Source: SGD pseudohyphal growth Inferred from mutant phenotype. Source: SGD regulation of exit from mitosis Inferred from mutant phenotype. Source: SGD regulation of initiation of mating projection growth Inferred from mutant phenotype. Source: SGD
Cellular component	cellular bud neck Traceable author statement. Source: SGD cellular bud tip Traceable author statement. Source: SGD intracellular Inferred from electronic annotation. Source: InterPro mating projection tip Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD soluble fraction Inferred from direct assay. Source: SGD
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Traceable author statement. Source: SGD protein binding Inferred from physical interaction. Source: IntAct signal transducer activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADH2	P00331	1	EBI-4274,EBI-2222
RDI1	Q12434	1	EBI-4274,EBI-7525
STE20	Q03497	2	EBI-4274,EBI-18285

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Cell division control protein 42		PRO_0000198955
	Propeptide	189 – 191	3	Removed in mature form By similarity		PRO_0000281285
Regions
	Nucleotide binding	10 – 17	8	GTP By similarity
	Nucleotide binding	57 – 61	5	GTP By similarity
	Nucleotide binding	115 – 118	4	GTP By similarity
	Motif	32 – 40	9	Effector region Potential
Amino acid modifications
	Modified residue	188	1	Cysteine methyl ester By similarity
	Lipidation	188	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	12	1	G → V: Enhances interaction with RGA1.
	Mutagenesis	58	1	T → A: Temperature-sensitive mutant.
	Mutagenesis	61	1	Q → L: Enhances interaction with RGA1.
	Mutagenesis	71	1	S → P: Temperature-sensitive mutant.
	Mutagenesis	97	1	W → R: Temperature-sensitive mutant.
	Mutagenesis	118	1	D → A: Dramatically reduces interaction with RGA1.
	Mutagenesis	142	1	G → S in CDC42-1; temperature-sensitive mutant.
	Mutagenesis	188	1	C → S: Enhances interaction with RGA1.
	Sequence conflict	189	1	A → T in CAA36186. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P19073-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 30BD644BA9836B2C	FASTA	191	21,323
	10 20 30 40 50 60 MQTLKCVVVG DGAVGKTCLL ISYTTNQFPA DYVPTVFDNY AVTVMIGDEP YTLGLFDTAG 70 80 90 100 110 120 QEDYDRLRPL SYPSTDVFLV CFSVISPPSF ENVKEKWFPE VHHHCPGVPC LVVGTQIDLR 130 140 150 160 170 180 DDKVIIEKLQ RQRLRPITSE QGSRLARELK AVKYVECSAL TQRGLKNVFD EAIVAALEPP 190 VIKKSKKCAI L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of CDC42, a Saccharomyces cerevisiae gene involved in the development of cell polarity." Johnson D.I., Pringle J.R. J. Cell Biol. 111:143-152(1990) [PubMed: 2164028] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Characterization of the Saccharomyces cerevisiae cdc42-1ts allele and new temperature-conditional-lethal cdc42 alleles." Miller P.J., Johnson D.I. Yeast 13:561-572(1997) [PubMed: 9178507] [Abstract] Cited for: MUTAGENESIS.
[5]	"Mutation of RGA1, which encodes a putative GTPase-activating protein for the polarity-establishment protein Cdc42p, activates the pheromone-response pathway in the yeast Saccharomyces cerevisiae." Stevenson B.J., Ferguson B., de Virgilio C., Bi E., Pringle J.R., Ammerer G., Sprague G.F. Jr. Genes Dev. 9:2949-2963(1995) [PubMed: 7498791] [Abstract] Cited for: INTERACTION WITH RGA1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51906 Genomic DNA. Translation: CAA36186.1. U19027 Genomic DNA. Translation: AAB67416.1. AY557933 Genomic DNA. Translation: AAS56259.1.
PIR	S51452.
RefSeq	NP_013330.1.
3D structure databases
HSSP	HSSP built from PDB template 1AM4 based on UniProtKB P21181.
SMR	P19073. Positions 1-191.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:862N.
IntAct	P19073. 7 interactions.
Proteomic databases
PeptideAtlas	P19073.
PRIDE	P19073.
Genome annotation databases
Ensembl	YLR229C. Saccharomyces cerevisiae. [Contig view]
GeneID	850930.
GenomeReviews	Gene locus YLR229C in contig Y13138_GR.
KEGG	sce:YLR229C.
NMPDR	fig|4932.3.peg.4343.
Organism-specific databases
CYGD	YLR229c.
SGD	S000004219. CDC42.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P19073.
OMA	P19073. EITHHCQ.
Gene expression databases
GermOnline	YLR229C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003578. GTPase_Rho. IPR013753. Ras. IPR001806. Ras_GTPase. IPR005225. Small_GTP_bd. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00174. RHO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51420. RHO. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967367.

Entry information

Entry name

CDC42_YEAST

Accession

Primary (citable) accession number: P19073
Secondary accession number(s): Q05978

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)