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Protein

Nitrogenase molybdenum-iron protein alpha chain

Gene

nifD1

more
Organism
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi72Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi98Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi164Iron-sulfur (8Fe-7S); shared with beta chainBy similarity1
Metal bindingi287Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl)By similarity1
Metal bindingi454Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
LigandATP-binding, Iron, Iron-sulfur, Metal-binding, Molybdenum, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein alpha chain (EC:1.18.6.1)
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene namesi
Name:nifD1
Ordered Locus Names:NGR_a01120
ORF Names:y4vL
AND
Name:nifD2
Ordered Locus Names:NGR_a00880
ORF Names:y4xB
Encoded oniPlasmid sym pNGR234a0 Publication
OrganismiSinorhizobium fredii (strain NBRC 101917 / NGR234)
Taxonomic identifieri394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001054 Componenti: Plasmid sym pNGR234a

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001530791 – 504Nitrogenase molybdenum-iron protein alpha chainAdd BLAST504

Proteomic databases

PRIDEiP19066

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Structurei

3D structure databases

ProteinModelPortaliP19066
SMRiP19066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.Curated

Phylogenomic databases

HOGENOMiHOG000005528
KOiK02586
OMAiYCFSTDM
OrthoDBiPOG091H01AN

Family and domain databases

CDDicd01976 Nitrogenase_MoFe_alpha, 1 hit
InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR010143 Nase_comp1_asu
IPR000318 Nase_comp1_CS
IPR005972 Nase_Mo-Fe_asu
PANTHERiPTHR43457 PTHR43457, 1 hit
PfamiView protein in Pfam
PF00148 Oxidored_nitro, 1 hit
TIGRFAMsiTIGR01862 N2-ase-Ialpha, 1 hit
TIGR01282 nifD, 1 hit
PROSITEiView protein in PROSITE
PS00699 NITROGENASE_1_1, 1 hit
PS00090 NITROGENASE_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P19066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLDYENDSA LHQELITQVL SQYPHKAAKR RQKHLSVASD REAVGEEGET
60 70 80 90 100
LSECDVKSNI KSIPGVMTIR GCAYAGSKGV VWGPVKDMVH ISHGPVGCGQ
110 120 130 140 150
YSWSQRRNYY VGTTGVDTFV TMQFTSDFQE KDIVFGGDKK LEQVIDEIEE
160 170 180 190 200
LFPLNNGITI QSECPIGLIG DDIEAVSRKK AAEHETTIVP VRCEGFRGVS
210 220 230 240 250
QSLGHHIAND AIRDWVFDKA DGKTDVEFET GPYDVNVIGD YNIGGDAWAS
260 270 280 290 300
RILLEEIGLR VVGNWSGDAT LAEVERAPRA KLNLIHCYRS MNYICRHMEE
310 320 330 340 350
RYAIPWMEYN FFGPSQIEAS LRKIARHFGP TIEERAERVI AKYRPLVDAV
360 370 380 390 400
IDKYWPRLQG KRVMLYVGGL RPRHVITAYE DLGMQIVGTG YEFAHNDDYQ
410 420 430 440 450
RTGHYVKTGT LIYDDATSYE LDTFIERIRP DLVGSGIKEK YPVQKMGIPF
460 470 480 490 500
RQMHSWDYSG PYHGYDGFAI FARDMDLAIN NPVWDLYDAP WKKMTVPTAA

VAAE
Length:504
Mass (Da):56,944
Last modified:November 1, 1997 - v2
Checksum:iD3BE3291A9D276DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201Q → K in AAA26328 (PubMed:2744485).Curated1
Sequence conflicti303A → G in AAA26328 (PubMed:2744485).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00090 Genomic DNA Translation: AAB91900.1
U00090 Genomic DNA Translation: AAB91924.1
M26961 Genomic DNA Translation: AAA26330.1
M26962 Genomic DNA Translation: AAA26328.1
PIRiPS0045
T10829
RefSeqiNP_444113.1, NC_000914.2
NP_444137.1, NC_000914.2
WP_010875129.1, NC_000914.2

Genome annotation databases

EnsemblBacteriaiAAB91900; AAB91900; NGR_a01120
AAB91924; AAB91924; NGR_a00880
GeneIDi962482
962502
KEGGirhi:NGR_a00880
rhi:NGR_a01120
PATRICifig|394.7.peg.77

Similar proteinsi

Entry informationi

Entry nameiNIFD_SINFN
AccessioniPrimary (citable) accession number: P19066
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

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