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P19066 (NIFD_RHISN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrogenase molybdenum-iron protein alpha chain

EC=1.18.6.1
Alternative name(s):
Dinitrogenase
Nitrogenase component I
Gene names
Name:nifD1
Ordered Locus Names:NGR_a01120
ORF Names:y4vL
AND
Name:nifD2
Ordered Locus Names:NGR_a00880
ORF Names:y4xB
Encoded onPlasmid sym pNGR234a
OrganismRhizobium sp. (strain NGR234) [Complete proteome] [HAMAP]
Taxonomic identifier394 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Catalytic activity

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.

Cofactor

Binds 1 8Fe-7S cluster per heterodimer By similarity.

Binds 1 7Fe-Mo-9S-C-homocitryl cluster per subunit By similarity.

Subunit structure

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Sequence similarities

Belongs to the NifD/NifK/NifE/NifN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Nitrogenase molybdenum-iron protein alpha chain
PRO_0000153079

Sites

Metal binding721Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding981Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding1641Iron-sulfur (8Fe-7S); shared with beta chain By similarity
Metal binding2871Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl) By similarity
Metal binding4541Molybdenum-iron-sulfur-carbon (7Fe-Mo-9S-C-homocitryl); via pros nitrogen By similarity

Experimental info

Sequence conflict2011Q → K in AAA26328. Ref.3
Sequence conflict3031A → G in AAA26328. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P19066 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: D3BE3291A9D276DC

FASTA50456,944
        10         20         30         40         50         60 
MSLDYENDSA LHQELITQVL SQYPHKAAKR RQKHLSVASD REAVGEEGET LSECDVKSNI 

        70         80         90        100        110        120 
KSIPGVMTIR GCAYAGSKGV VWGPVKDMVH ISHGPVGCGQ YSWSQRRNYY VGTTGVDTFV 

       130        140        150        160        170        180 
TMQFTSDFQE KDIVFGGDKK LEQVIDEIEE LFPLNNGITI QSECPIGLIG DDIEAVSRKK 

       190        200        210        220        230        240 
AAEHETTIVP VRCEGFRGVS QSLGHHIAND AIRDWVFDKA DGKTDVEFET GPYDVNVIGD 

       250        260        270        280        290        300 
YNIGGDAWAS RILLEEIGLR VVGNWSGDAT LAEVERAPRA KLNLIHCYRS MNYICRHMEE 

       310        320        330        340        350        360 
RYAIPWMEYN FFGPSQIEAS LRKIARHFGP TIEERAERVI AKYRPLVDAV IDKYWPRLQG 

       370        380        390        400        410        420 
KRVMLYVGGL RPRHVITAYE DLGMQIVGTG YEFAHNDDYQ RTGHYVKTGT LIYDDATSYE 

       430        440        450        460        470        480 
LDTFIERIRP DLVGSGIKEK YPVQKMGIPF RQMHSWDYSG PYHGYDGFAI FARDMDLAIN 

       490        500 
NPVWDLYDAP WKKMTVPTAA VAAE 

« Hide

References

« Hide 'large scale' references
[1]"Molecular basis of symbiosis between Rhizobium and legumes."
Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A., Perret X.
Nature 387:394-401(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[2]"Rhizobium sp. strain NGR234 possesses a remarkable number of secretion systems."
Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A., Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A., Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A., Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.
Appl. Environ. Microbiol. 75:4035-4045(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NGR234.
[3]"Structural and functional analysis of nitrogenase genes from the broad-host-range Rhizobium strain ANU240."
Badenoch-Jones J., Holton T.A., Morrison C.M., Scott K.F., Shine J.
Gene 77:141-153(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16 AND 199-393.
Strain: ANU 240.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00090 Genomic DNA. Translation: AAB91900.1.
U00090 Genomic DNA. Translation: AAB91924.1.
M26961 Genomic DNA. Translation: AAA26330.1.
M26962 Genomic DNA. Translation: AAA26328.1.
PIRPS0045.
T10829.
RefSeqNP_444113.1. NC_000914.2.
NP_444137.1. NC_000914.2.

3D structure databases

ProteinModelPortalP19066.
SMRP19066. Positions 13-492.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING394.NGR_a01120.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB91900; AAB91900; NGR_a01120.
AAB91924; AAB91924; NGR_a00880.
GeneID962482.
962502.
KEGGrhi:NGR_a00880.
rhi:NGR_a01120.
PATRIC32300883. VBIRhiSp122450_0077.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2710.
HOGENOMHOG000005528.
KOK02586.
OMASTCPVGL.
OrthoDBEOG6VHZ72.
ProtClustDBCLSK864414.

Enzyme and pathway databases

BioCycSFRE394:GBYN-6139-MONOMER.
SFRE394:GBYN-6163-MONOMER.

Family and domain databases

InterProIPR000510. Nase/OxRdtase_comp1.
IPR010143. Nase_comp1_asu.
IPR000318. Nase_comp1_CS.
IPR005972. Nase_Mo-Fe_asu.
[Graphical view]
PfamPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
TIGRFAMsTIGR01862. N2-ase-Ialpha. 1 hit.
TIGR01282. nifD. 1 hit.
PROSITEPS00699. NITROGENASE_1_1. 1 hit.
PS00090. NITROGENASE_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIFD_RHISN
AccessionPrimary (citable) accession number: P19066
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: January 22, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rhizobium plasmid pNGR234a

Rhizobium plasmid pNGR234a: entries and gene names