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Protein

Baseplate wedge protein gp8

Gene

8

Organism
Enterobacteria phage T4 (Bacteriophage T4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Viral tail assembly, Virus exit from host cell

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Baseplate wedge protein gp8Curated
Alternative name(s):
Gene product 8
Short name:
gp8
Gene namesi
Name:8
OrganismiEnterobacteria phage T4 (Bacteriophage T4)
Taxonomic identifieri10665 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeTevenvirinaeT4likevirus
Virus hostiEscherichia coli [TaxID: 562]
ProteomesiUP000009087 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication

  • Note: Present in 12 copies in the baseplate.1 Publication

GO - Cellular componenti

  • virion Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Viral baseplate protein, Viral tail protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Baseplate wedge protein gp8PRO_0000164998Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi142 ↔ 153

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.Curated

Interactioni

Subunit structurei

Homodimer (PubMed:2403438, PubMed:19896486). Part of the baseplate macromolecular complex which consists of gp5, gp26, gp27, gp28, gp29 (hub); gp6, gp7, gp8, gp10, gp11, gp25, gp53 (wedge); gp9, gp12, gp48 and gp54.1 Publication2 Publications

Structurei

Secondary structure

1
334
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 124Combined sources
Helixi14 – 2714Combined sources
Beta strandi28 – 314Combined sources
Beta strandi37 – 426Combined sources
Helixi49 – 524Combined sources
Helixi65 – 7410Combined sources
Beta strandi75 – 817Combined sources
Helixi83 – 853Combined sources
Beta strandi86 – 916Combined sources
Beta strandi111 – 1177Combined sources
Turni118 – 1203Combined sources
Beta strandi128 – 1358Combined sources
Beta strandi138 – 1447Combined sources
Helixi150 – 1556Combined sources
Beta strandi160 – 1645Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi183 – 1853Combined sources
Beta strandi187 – 1948Combined sources
Helixi196 – 2027Combined sources
Beta strandi205 – 2106Combined sources
Helixi213 – 2186Combined sources
Helixi220 – 2234Combined sources
Helixi231 – 2344Combined sources
Helixi238 – 2414Combined sources
Beta strandi246 – 2494Combined sources
Beta strandi251 – 2544Combined sources
Turni255 – 2573Combined sources
Helixi259 – 2624Combined sources
Beta strandi269 – 2779Combined sources
Beta strandi294 – 2974Combined sources
Helixi299 – 3013Combined sources
Beta strandi307 – 31812Combined sources
Beta strandi325 – 3284Combined sources
Beta strandi331 – 3333Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N7ZX-ray2.00A/B/C/D1-334[»]
1N80X-ray2.45A/B/C/D1-334[»]
1N8BX-ray2.90A/B/C/D1-334[»]
1PDMelectron microscopy12.00A/B/C/D/E/F/G/H/I/J/K/L1-334[»]
1TJAelectron microscopy16.00A/B1-334[»]
ProteinModelPortaliP19062.
SMRiP19062. Positions 7-334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19062.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR015298. Phage_T4_Gp8.
[Graphical view]
PfamiPF09215. Phage-Gp8. 1 hit.
[Graphical view]
SUPFAMiSSF89433. SSF89433. 1 hit.

Sequencei

Sequence statusi: Complete.

P19062-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDSSVIYRA IVTSKFRTEK MLNFYNSIGS GPDKNTIFIT FGRSEPWSSN
60 70 80 90 100
ENEVGFAPPY PTDSVLGVTD MWTHMMGTVK VLPSMLDAVI PRRDWGDTRY
110 120 130 140 150
PDPYTFRIND IVVCNSAPYN ATESGAGWLV YRCLDVPDTG MCSIASLTDK
160 170 180 190 200
DECLKLGGKW TPSARSMTPP EGRGDAEGTI EPGDGYVWEY LFEIPPDVSI
210 220 230 240 250
NRCTNEYIVV PWPEELKEDP TRWGYEDNLT WQQDDFGLIY RVKANTIRFK
260 270 280 290 300
AYLDSVYFPE AALPGNKGFR QISIITNPLE AKAHPNDPNV KAEKDYYDPE
310 320 330
DLMRHSGEMI YMENRPPIIM AMDQTEEINI LFTF
Length:334
Mass (Da):38,008
Last modified:November 1, 1990 - v1
Checksum:i4997860773E14899
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15907 Genomic DNA. Translation: CAA34023.1.
AF158101 Genomic DNA. Translation: AAD42519.1.
PIRiJQ0658. G8BPT4.
RefSeqiNP_049766.1. NC_000866.4.

Genome annotation databases

GeneIDi1258542.
KEGGivg:1258542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15907 Genomic DNA. Translation: CAA34023.1.
AF158101 Genomic DNA. Translation: AAD42519.1.
PIRiJQ0658. G8BPT4.
RefSeqiNP_049766.1. NC_000866.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N7ZX-ray2.00A/B/C/D1-334[»]
1N80X-ray2.45A/B/C/D1-334[»]
1N8BX-ray2.90A/B/C/D1-334[»]
1PDMelectron microscopy12.00A/B/C/D/E/F/G/H/I/J/K/L1-334[»]
1TJAelectron microscopy16.00A/B1-334[»]
ProteinModelPortaliP19062.
SMRiP19062. Positions 7-334.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.K.1.1.1. the gp27/5 t4-baseplate (t4-bp) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1258542.
KEGGivg:1258542.

Miscellaneous databases

EvolutionaryTraceiP19062.

Family and domain databases

InterProiIPR015298. Phage_T4_Gp8.
[Graphical view]
PfamiPF09215. Phage-Gp8. 1 hit.
[Graphical view]
SUPFAMiSSF89433. SSF89433. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of bacteriophage T4 genes 6, 7 and 8."
    Efimov V.P., Prilipov A.G., Mesyanzhinov V.V.
    Nucleic Acids Res. 18:5313-5313(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: D.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Structure of the bacteriophage T4 baseplate as determined by chemical cross-linking."
    Watts N.R., Coombs D.H.
    J. Virol. 64:143-154(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. Cited for: REVIEW.
  5. Cited for: REVIEW ON FUNCTION.
  6. "The baseplate wedges of bacteriophage T4 spontaneously assemble into hubless baseplate-like structure in vitro."
    Yap M.L., Mio K., Leiman P.G., Kanamaru S., Arisaka F.
    J. Mol. Biol. 395:349-360(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  7. "Structure and location of gene product 8 in the bacteriophage T4 baseplate."
    Leiman P.G., Shneider M.M., Kostyuchenko V.A., Chipman P.R., Mesyanzhinov V.V., Rossmann M.G.
    J. Mol. Biol. 328:821-833(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 7-334.
  8. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host."
    Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V., Rossmann M.G.
    Cell 118:419-429(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (17.0 ANGSTROMS) OF THE CONTRACTED TAIL, SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiBP08_BPT4
AccessioniPrimary (citable) accession number: P19062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 27, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.