P19062 (VG08_BPT4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Baseplate structural protein Gp8

Alternative name(s):
Baseplate wedge protein 8

Gene names

Name:

8

Organism

Enterobacteria phage T4 (Bacteriophage T4)

Taxonomic identifier

10665 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › T4-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Structural component of the baseplate.
Subunit structure	Homodimer. There are 6 Gp8 dimers within the baseplate-tail tube complex.
Subcellular location	Virion Potential.

Ontologies

Keywords
Cellular component	Virion
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 334

334

Baseplate structural protein Gp8

PRO_0000164998

Amino acid modifications

Disulfide bond

142 ↔ 153

Secondary structure

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334

Helix Strand Turn

Details...

Beta strand

9 – 12

4

Helix

14 – 27

14

Beta strand

28 – 31

4

Beta strand

37 – 42

6

Helix

49 – 52

4

Helix

65 – 74

10

Beta strand

75 – 81

7

Helix

83 – 85

3

Beta strand

86 – 91

6

Beta strand

111 – 117

7

Turn

118 – 120

3

Beta strand

128 – 135

8

Beta strand

138 – 144

7

Helix

150 – 155

6

Beta strand

160 – 164

5

Beta strand

183 – 185

3

Beta strand

187 – 194

8

Helix

196 – 202

7

Beta strand

205 – 210

6

Helix

213 – 218

6

Helix

220 – 223

4

Helix

231 – 234

4

Helix

238 – 241

4

Beta strand

246 – 249

4

Beta strand

251 – 254

4

Turn

255 – 257

3

Helix

259 – 262

4

Beta strand

269 – 277

9

Beta strand

294 – 297

4

Helix

299 – 301

3

Beta strand

307 – 318

12

Beta strand

325 – 328

4

Beta strand

331 – 333

3

Sequences

	Sequence		Length	Mass (Da)
	P19062 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 4997860773E14899	FASTA	334	38,008
	10 20 30 40 50 60 MNDSSVIYRA IVTSKFRTEK MLNFYNSIGS GPDKNTIFIT FGRSEPWSSN ENEVGFAPPY 70 80 90 100 110 120 PTDSVLGVTD MWTHMMGTVK VLPSMLDAVI PRRDWGDTRY PDPYTFRIND IVVCNSAPYN 130 140 150 160 170 180 ATESGAGWLV YRCLDVPDTG MCSIASLTDK DECLKLGGKW TPSARSMTPP EGRGDAEGTI 190 200 210 220 230 240 EPGDGYVWEY LFEIPPDVSI NRCTNEYIVV PWPEELKEDP TRWGYEDNLT WQQDDFGLIY 250 260 270 280 290 300 RVKANTIRFK AYLDSVYFPE AALPGNKGFR QISIITNPLE AKAHPNDPNV KAEKDYYDPE 310 320 330 DLMRHSGEMI YMENRPPIIM AMDQTEEINI LFTF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of bacteriophage T4 genes 6, 7 and 8." Efimov V.P., Prilipov A.G., Mesyanzhinov V.V. Nucleic Acids Res. 18:5313-5313(1990) [PubMed: 2402473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D.
[2]	"Bacteriophage T4 genome." Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W. Microbiol. Mol. Biol. Rev. 67:86-156(2003) [PubMed: 12626685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Structure and location of gene product 8 in the bacteriophage T4 baseplate." Leiman P.G., Shneider M.M., Kostyuchenko V.A., Chipman P.R., Mesyanzhinov V.V., Rossmann M.G. J. Mol. Biol. 328:821-833(2003) [PubMed: 12729757] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 7-334.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X15907 Genomic DNA. Translation: CAA34023.1.
AF158101 Genomic DNA. Translation: AAD42519.1.

PIR

G8BPT4. JQ0658.

RefSeq

NP_049766.1. NC_000866.4.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1N7Z	X-ray	2.00	A/B/C/D	1-334	[»]
1N80	X-ray	2.45	A/B/C/D	1-334	[»]
1N8B	X-ray	2.90	A/B/C/D	1-334	[»]
1PDM	electron microscopy	12.00	A/B/C/D/E/F/G/H/I/J/K/L	1-334	[»]
1TJA	electron microscopy	16.00	A/B	1-334	[»]

ProteinModelPortal

P19062.

SMR

P19062. Positions 7-334.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1258542.

GenomeReviews

Gene locus T4p154 in contig AF158101_GR.

Phylogenomic databases

ProtClustDB

PHA2580.

Family and domain databases

InterPro

IPR015298. Phage_T4_Gp8.
[Graphical view]

Pfam

PF09215. Phage-Gp8. 1 hit.
[Graphical view]

SUPFAM

SSF89433. Phage_T4_Gp8. 1 hit.

ProtoNet

Search...

Entry information

Entry name

VG08_BPT4

Accession

Primary (citable) accession number: P19062

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program