Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pol polyprotein

Gene

pol

Organism
Feline immunodeficiency virus (strain San Diego) (FIV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Caution

The N-terminus of the polypeptide is uncertain.Curated

Catalytic activityi

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
dUTP + H2O = dUMP + diphosphate.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68For protease activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri848 – 889Integrase-typePROSITE-ProRule annotationAdd BLAST42
DNA bindingi1067 – 1115Integrase-typePROSITE-ProRule annotationAdd BLAST49

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, DNA-binding, Endonuclease, Hydrolase, Multifunctional enzyme, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase
Biological processDNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 4 chains:
Alternative name(s):
Retropepsin
Alternative name(s):
Exoribonuclease H (EC:3.1.13.2)
Integrase (EC:2.7.7.-By similarity, EC:3.1.-.-By similarity)
Short name:
IN
Gene namesi
Name:pol
OrganismiFeline immunodeficiency virus (strain San Diego) (FIV)
Taxonomic identifieri11675 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
Virus hostiFelidae (cat family) [TaxID: 9681]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000388451 – 154ProteaseBy similarityAdd BLAST154
ChainiPRO_0000038846155 – 710Reverse transcriptase/ribonuclease HBy similarityAdd BLAST556
ChainiPRO_0000038847711 – 843Deoxyuridine 5'-triphosphate nucleotidohydrolaseBy similarityAdd BLAST133
ChainiPRO_0000038848844 – 1124IntegraseBy similarityAdd BLAST281

Post-translational modificationi

Cleavage sites that yield the mature proteins remain to be determined.

Proteomic databases

PRIDEiP19028

Structurei

3D structure databases

ProteinModelPortaliP19028
SMRiP19028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 144Peptidase A2PROSITE-ProRule annotationAdd BLAST82
Domaini200 – 389Reverse transcriptasePROSITE-ProRule annotationAdd BLAST190
Domaini592 – 712RNase HPROSITE-ProRule annotationAdd BLAST121
Domaini899 – 1049Integrase catalyticPROSITE-ProRule annotationAdd BLAST151

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri848 – 889Integrase-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Zinc-finger

Family and domain databases

CDDicd05482 HIV_retropepsin_like, 1 hit
cd07557 trimeric_dUTPase, 1 hit
Gene3Di1.10.10.200, 1 hit
2.30.30.10, 1 hit
2.40.70.10, 1 hit
2.70.40.10, 1 hit
3.30.420.10, 2 hits
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
IPR017856 Integrase-like_N
IPR036862 Integrase_C_dom_sf_retrovir
IPR001037 Integrase_C_retrovir
IPR001584 Integrase_cat-core
IPR003308 Integrase_Zn-bd_dom_N
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR034170 Retropepsin-like_cat_dom
IPR018061 Retropepsins
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
IPR000477 RT_dom
IPR010659 RVT_connect
IPR010661 RVT_thumb
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
PF00552 IN_DBD_C, 1 hit
PF02022 Integrase_Zn, 1 hit
PF00075 RNase_H, 1 hit
PF00665 rve, 1 hit
PF00077 RVP, 1 hit
PF00078 RVT_1, 1 hit
PF06815 RVT_connect, 1 hit
PF06817 RVT_thumb, 1 hit
SUPFAMiSSF46919 SSF46919, 1 hit
SSF50122 SSF50122, 1 hit
SSF50630 SSF50630, 1 hit
SSF51283 SSF51283, 1 hit
SSF53098 SSF53098, 2 hits
TIGRFAMsiTIGR00576 dut, 1 hit
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50994 INTEGRASE, 1 hit
PS51027 INTEGRASE_DBD, 1 hit
PS50879 RNASE_H, 1 hit
PS50878 RT_POL, 1 hit
PS50876 ZF_INTEGRASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KKFGKLEGGA SCSPSESSAA NSNAICTSNG GKIIGFINYN KVGTTTSLEK
60 70 80 90 100
RPEILIFVNG YPIKFLLDTG ADITILNRRD FQVKNSIENG RQNMIGVGGG
110 120 130 140 150
KRGTNYINVH LEIRDENYKT QCIFGNVCVL EDNSLIQPLL GRDNMIKFNI
160 170 180 190 200
RLVMAQISDK IPIVKVKMKD PNKGPQIKQW PLSNEKIEAL TEIVERLERE
210 220 230 240 250
GKVKRADPNN PWNTPVFAIK KKSGKWRMLI DFRELNKLTE KGAEVQLGLP
260 270 280 290 300
HPAGLQMKKQ ITVLDIGDAY FTNPLDPDYA PYTAFTLPRK NNAGPGRRFV
310 320 330 340 350
WCSLPQGWIL SPLIYQSTLD NIIQPFIRQN PQLDIYQYMD DIYIGSNLSK
360 370 380 390 400
KEHKEKVEEL RKLLLWWGFE TPEDKLQEEP PYKWMGYELH PLTWTIQQKQ
410 420 430 440 450
LEIPEKPTLN ELQKLAGKIN WASQTIPELS IKSLTNMTRG NQNLNSTREW
460 470 480 490 500
TEEARLEVQK AKRAIEEQVQ LGYYDPSKEL YAKLSLVGPH QISYQVYQKC
510 520 530 540 550
PEKILWYGKM SRQKKKAENT CDIALRACYK IREESIIRIG KEPRYEIPTS
560 570 580 590 600
REAWESNLIN SPYLKAPPPE VDYIHAALNI KRALSMIKDP PISGAETWYI
610 620 630 640 650
DGGRKLGKAA KAAYWTDTGK WQVMELEGSN QKAEIQALLL ALKAGPEEMN
660 670 680 690 700
IITDSQYMIN ILSQQPDKME GIWQEVLEEL EKKTAIFIDW VPGHKGIPGN
710 720 730 740 750
EEVDKLCQTM MIIEGDGILD KRTEDAGYDL LAAKEIHLLP GEVKVIPTGV
760 770 780 790 800
KLMLPKGHWG LIMGKSSIGS KGLDVLGGVI DEGYRGEIGV IMINLSKKSI
810 820 830 840 850
TLLEQQKIAQ LIILPHKHEA LEQGKVVMDS ERGEKGYGST GVFSSWVDRI
860 870 880 890 900
EEAETNHEKF HSDPQYLRTE FNLPKMVAEE IRRKCPVCRI RGEQVGGQLK
910 920 930 940 950
IGPGIWQMDC THFDGKIILV AIHVESGYIW AQIISQETAD CTVKAVLQLL
960 970 980 990 1000
SAHIVTELQT DNGPNFKNQK MEGVLNYMGV KHKFGIPGNP QSQALVENVN
1010 1020 1030 1040 1050
QTLKVWVHKF LPETTSLDNA LALAVHCLNF KQRGRIGGMA PYELLAQQES
1060 1070 1080 1090 1100
LRIQDYFSAI PQKLQAQWIY YKDQKDKKWK GPMRVEYWGQ GSVLLKDEEK
1110 1120
GYFLIPRRHV KRVPEPCALP EGDE
Length:1,124
Mass (Da):127,605
Last modified:November 1, 1990 - v1
Checksum:i1A4706685CFDA8CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36968 Genomic RNA Translation: AAA43076.1

Similar proteinsi

Entry informationi

Entry nameiPOL_FIVSD
AccessioniPrimary (citable) accession number: P19028
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health