Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P19028

- POL_FIVSD

UniProt

P19028 - POL_FIVSD

Protein

Pol polyprotein

Gene

pol

Organism
Feline immunodeficiency virus (strain San Diego) (FIV)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

    Catalytic activityi

    Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
    3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
    dUTP + H2O = dUMP + diphosphate.
    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681For protease activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri848 – 88942Integrase-typePROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi1067 – 111549Integrase-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. dUTP diphosphatase activity Source: UniProtKB-EC
    4. exoribonuclease H activity Source: UniProtKB-EC
    5. RNA binding Source: InterPro
    6. RNA-directed DNA polymerase activity Source: UniProtKB-KW
    7. RNA-DNA hybrid ribonuclease activity Source: InterPro
    8. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. DNA recombination Source: UniProtKB-KW
    3. dUTP metabolic process Source: InterPro
    4. establishment of integrated proviral latency Source: UniProtKB-KW
    5. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

    Keywords - Biological processi

    DNA integration, DNA recombination, Nucleotide metabolism, Viral genome integration, Virus entry into host cell

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pol polyprotein
    Cleaved into the following 4 chains:
    Alternative name(s):
    Retropepsin
    Alternative name(s):
    Exoribonuclease H (EC:3.1.13.2)
    Integrase
    Short name:
    IN
    Gene namesi
    Name:pol
    OrganismiFeline immunodeficiency virus (strain San Diego) (FIV)
    Taxonomic identifieri11675 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeLentivirusFeline lentivirus group
    Virus hostiFelidae (cat family) [TaxID: 9681]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 154154ProteaseBy similarityPRO_0000038845Add
    BLAST
    Chaini155 – 710556Reverse transcriptase/ribonuclease HBy similarityPRO_0000038846Add
    BLAST
    Chaini711 – 843133Deoxyuridine 5'-triphosphate nucleotidohydrolaseBy similarityPRO_0000038847Add
    BLAST
    Chaini844 – 1124281IntegraseBy similarityPRO_0000038848Add
    BLAST

    Post-translational modificationi

    Cleavage sites that yield the mature proteins remain to be determined.

    Structurei

    3D structure databases

    ProteinModelPortaliP19028.
    SMRiP19028. Positions 42-154, 711-843.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 14482Peptidase A2PROSITE-ProRule annotationAdd
    BLAST
    Domaini200 – 389190Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST
    Domaini592 – 712121RNase HPROSITE-ProRule annotationAdd
    BLAST
    Domaini899 – 1049151Integrase catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the retroviral Pol polyprotein family.Curated
    Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
    Contains 1 integrase-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 integrase-type zinc finger.PROSITE-ProRule annotation
    Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
    Contains 1 RNase H domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri848 – 88942Integrase-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.10.200. 1 hit.
    2.30.30.10. 1 hit.
    2.40.70.10. 1 hit.
    2.70.40.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProiIPR001969. Aspartic_peptidase_AS.
    IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    IPR001037. Integrase_C_retrovir.
    IPR001584. Integrase_cat-core.
    IPR017856. Integrase_Zn-bd_dom-like_N.
    IPR003308. Integrase_Zn-bd_dom_N.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    IPR010659. RVT_connect.
    IPR010661. RVT_thumb.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    PF00552. IN_DBD_C. 1 hit.
    PF02022. Integrase_Zn. 1 hit.
    PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    PF06815. RVT_connect. 1 hit.
    PF06817. RVT_thumb. 1 hit.
    [Graphical view]
    SUPFAMiSSF46919. SSF46919. 1 hit.
    SSF50122. SSF50122. 1 hit.
    SSF50630. SSF50630. 1 hit.
    SSF51283. SSF51283. 1 hit.
    SSF53098. SSF53098. 2 hits.
    TIGRFAMsiTIGR00576. dut. 1 hit.
    PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS51027. INTEGRASE_DBD. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    PS50876. ZF_INTEGRASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P19028-1 [UniParc]FASTAAdd to Basket

    « Hide

    KKFGKLEGGA SCSPSESSAA NSNAICTSNG GKIIGFINYN KVGTTTSLEK     50
    RPEILIFVNG YPIKFLLDTG ADITILNRRD FQVKNSIENG RQNMIGVGGG 100
    KRGTNYINVH LEIRDENYKT QCIFGNVCVL EDNSLIQPLL GRDNMIKFNI 150
    RLVMAQISDK IPIVKVKMKD PNKGPQIKQW PLSNEKIEAL TEIVERLERE 200
    GKVKRADPNN PWNTPVFAIK KKSGKWRMLI DFRELNKLTE KGAEVQLGLP 250
    HPAGLQMKKQ ITVLDIGDAY FTNPLDPDYA PYTAFTLPRK NNAGPGRRFV 300
    WCSLPQGWIL SPLIYQSTLD NIIQPFIRQN PQLDIYQYMD DIYIGSNLSK 350
    KEHKEKVEEL RKLLLWWGFE TPEDKLQEEP PYKWMGYELH PLTWTIQQKQ 400
    LEIPEKPTLN ELQKLAGKIN WASQTIPELS IKSLTNMTRG NQNLNSTREW 450
    TEEARLEVQK AKRAIEEQVQ LGYYDPSKEL YAKLSLVGPH QISYQVYQKC 500
    PEKILWYGKM SRQKKKAENT CDIALRACYK IREESIIRIG KEPRYEIPTS 550
    REAWESNLIN SPYLKAPPPE VDYIHAALNI KRALSMIKDP PISGAETWYI 600
    DGGRKLGKAA KAAYWTDTGK WQVMELEGSN QKAEIQALLL ALKAGPEEMN 650
    IITDSQYMIN ILSQQPDKME GIWQEVLEEL EKKTAIFIDW VPGHKGIPGN 700
    EEVDKLCQTM MIIEGDGILD KRTEDAGYDL LAAKEIHLLP GEVKVIPTGV 750
    KLMLPKGHWG LIMGKSSIGS KGLDVLGGVI DEGYRGEIGV IMINLSKKSI 800
    TLLEQQKIAQ LIILPHKHEA LEQGKVVMDS ERGEKGYGST GVFSSWVDRI 850
    EEAETNHEKF HSDPQYLRTE FNLPKMVAEE IRRKCPVCRI RGEQVGGQLK 900
    IGPGIWQMDC THFDGKIILV AIHVESGYIW AQIISQETAD CTVKAVLQLL 950
    SAHIVTELQT DNGPNFKNQK MEGVLNYMGV KHKFGIPGNP QSQALVENVN 1000
    QTLKVWVHKF LPETTSLDNA LALAVHCLNF KQRGRIGGMA PYELLAQQES 1050
    LRIQDYFSAI PQKLQAQWIY YKDQKDKKWK GPMRVEYWGQ GSVLLKDEEK 1100
    GYFLIPRRHV KRVPEPCALP EGDE 1124
    Length:1,124
    Mass (Da):127,605
    Last modified:November 1, 1990 - v1
    Checksum:i1A4706685CFDA8CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36968 Genomic RNA. Translation: AAA43076.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36968 Genomic RNA. Translation: AAA43076.1 .

    3D structure databases

    ProteinModelPortali P19028.
    SMRi P19028. Positions 42-154, 711-843.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.10.200. 1 hit.
    2.30.30.10. 1 hit.
    2.40.70.10. 1 hit.
    2.70.40.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProi IPR001969. Aspartic_peptidase_AS.
    IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    IPR001037. Integrase_C_retrovir.
    IPR001584. Integrase_cat-core.
    IPR017856. Integrase_Zn-bd_dom-like_N.
    IPR003308. Integrase_Zn-bd_dom_N.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    IPR010659. RVT_connect.
    IPR010661. RVT_thumb.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    PF00552. IN_DBD_C. 1 hit.
    PF02022. Integrase_Zn. 1 hit.
    PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    PF06815. RVT_connect. 1 hit.
    PF06817. RVT_thumb. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46919. SSF46919. 1 hit.
    SSF50122. SSF50122. 1 hit.
    SSF50630. SSF50630. 1 hit.
    SSF51283. SSF51283. 1 hit.
    SSF53098. SSF53098. 2 hits.
    TIGRFAMsi TIGR00576. dut. 1 hit.
    PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS51027. INTEGRASE_DBD. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    PS50876. ZF_INTEGRASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of two host cell range variants of feline immunodeficiency virus."
      Phillips T.R., Talbott R.L., Lamont C., Muir S., Lovelace K.M., Elder J.H.
      J. Virol. 64:4605-4613(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate PPR.

    Entry informationi

    Entry nameiPOL_FIVSD
    AccessioniPrimary (citable) accession number: P19028
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Caution

    The N-terminus of the polypeptide is uncertain.Curated

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3