Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P19024

- KCNA5_RAT

UniProt

P19024 - KCNA5_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Potassium voltage-gated channel subfamily A member 5

Gene

Kcna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. This channel displays rapid activation and slow inactivation.1 Publication

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: RefGenome
  2. outward rectifier potassium channel activity Source: RGD
  3. potassium channel inhibitor activity Source: Ensembl
  4. receptor binding Source: RGD
  5. voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Source: Ensembl

GO - Biological processi

  1. membrane hyperpolarization Source: Ensembl
  2. negative regulation of cytosolic calcium ion concentration Source: RGD
  3. negative regulation of potassium ion transport Source: Ensembl
  4. positive regulation of G1/S transition of mitotic cell cycle Source: RGD
  5. positive regulation of myoblast proliferation Source: RGD
  6. potassium ion export Source: Ensembl
  7. potassium ion homeostasis Source: RGD
  8. potassium ion transport Source: RGD
  9. protein homooligomerization Source: InterPro
  10. protein oligomerization Source: RGD
  11. regulation of atrial cardiac muscle cell membrane repolarization Source: Ensembl
  12. regulation of heart rate by cardiac conduction Source: Ensembl
  13. regulation of membrane potential Source: RGD
  14. regulation of vasoconstriction Source: Ensembl
  15. response to hydrogen peroxide Source: RGD
  16. response to hyperoxia Source: RGD
  17. response to hypoxia Source: RGD
  18. response to mechanical stimulus Source: RGD
  19. response to organic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_199159. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 5
Alternative name(s):
RCK7
RK4
Voltage-gated potassium channel subunit Kv1.5
Gene namesi
Name:Kcna5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi2953. Kcna5.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: RGD
  2. Golgi apparatus Source: RGD
  3. intercalated disc Source: RGD
  4. intracellular canaliculus Source: RGD
  5. membrane raft Source: Ensembl
  6. perinuclear region of cytoplasm Source: RGD
  7. plasma membrane Source: RGD
  8. voltage-gated potassium channel complex Source: Ensembl
  9. Z disc Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi600 – 6023TDL → AAA: Loss of interaction with DLG1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Potassium voltage-gated channel subfamily A member 5PRO_0000053989Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Modified residuei81 – 811Phosphoserine; by CK2 and PKASequence Analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Cross-linki212 – 212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Lipidationi337 – 3371S-palmitoyl cysteineSequence Analysis
Cross-linki525 – 525Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei535 – 5351Phosphoserine; by PKASequence Analysis
Modified residuei546 – 5461Phosphoserine; by PKASequence Analysis
Modified residuei569 – 5691Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Glycoprotein, Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP19024.
PRIDEiP19024.

Expressioni

Tissue specificityi

Expressed equally in atrium, ventricle, aorta and skeletal muscle. Weaker expression in brain.1 Publication

Inductioni

Expression regulated by cAMP in a tissue-specific manner. In primary cardiac cells, levels increase by 6-fold, and in GH3 cells, levels decrease 5-6-fold.

Gene expression databases

GenevestigatoriP19024.

Interactioni

Subunit structurei

Heterotetramer of potassium channel proteins (By similarity). Interacts with DLG1, which enhances channel currents. Forms a ternary complex with DLG1 and CAV3. Interacts with UBE2I (By similarity).By similarity

Protein-protein interaction databases

BioGridi247503. 4 interactions.
IntActiP19024. 2 interactions.
MINTiMINT-3973915.
STRINGi10116.ENSRNOP00000026691.

Structurei

3D structure databases

ProteinModelPortaliP19024.
SMRiP19024. Positions 111-516.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei242 – 26019Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei316 – 33621Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Transmembranei347 – 36822Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei387 – 40822Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Transmembranei423 – 44422Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Transmembranei484 – 50522Helical; Name=Segment S6Sequence AnalysisAdd
BLAST

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi469 – 4746Selectivity filterBy similarity
Motifi600 – 6023PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi373 – 3764Poly-Gly

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP19024.
KOiK04878.
OMAiGGELQCP.
OrthoDBiEOG7M0NRD.
PhylomeDBiP19024.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004052. K_chnl_volt-dep_Kv1.5.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PTHR11537:SF25. PTHR11537:SF25. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01512. KV15CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P19024-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEISLVPLEN GSAMTLRGGG EAGASCVQTP RGECGCPPTS GLNNQSKETL
60 70 80 90 100
LRGRTTLEDA NQGGRPLPPM AQELPQPRRL SAEDEEGEGD PGLGTVEEDQ
110 120 130 140 150
APQDAGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP AKRLHYFDPL
160 170 180 190 200
RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA
210 220 230 240 250
MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV
260 270 280 290 300
ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGIN GSVSGALSSG
310 320 330 340 350
PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI
360 370 380 390 400
IDVVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF
410 420 430 440 450
KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNHG
460 470 480 490 500
SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP
510 520 530 540 550
VPVIVSNFNY FYHRETDHEE QAALKEEQGN QRRESGLDTG GQRKVSCSKA
560 570 580 590 600
SFCKTGGSLE SSDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET

DL
Length:602
Mass (Da):66,553
Last modified:November 1, 1990 - v1
Checksum:i6A784535FF226ED7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti553 – 5531C → S no nucleotide entry (PubMed:1705709)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27158 Genomic DNA. Translation: AAA41498.1.
L23434 Genomic DNA. Translation: AAA42337.1.
PIRiJH0166.
RefSeqiNP_037104.1. NM_012972.1.
UniGeneiRn.162789.

Genome annotation databases

EnsembliENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719.
GeneIDi25470.
KEGGirno:25470.
UCSCiRGD:2953. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M27158 Genomic DNA. Translation: AAA41498.1 .
L23434 Genomic DNA. Translation: AAA42337.1 .
PIRi JH0166.
RefSeqi NP_037104.1. NM_012972.1.
UniGenei Rn.162789.

3D structure databases

ProteinModelPortali P19024.
SMRi P19024. Positions 111-516.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247503. 4 interactions.
IntActi P19024. 2 interactions.
MINTi MINT-3973915.
STRINGi 10116.ENSRNOP00000026691.

Proteomic databases

PaxDbi P19024.
PRIDEi P19024.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026691 ; ENSRNOP00000026691 ; ENSRNOG00000019719 .
GeneIDi 25470.
KEGGi rno:25470.
UCSCi RGD:2953. rat.

Organism-specific databases

CTDi 3741.
RGDi 2953. Kcna5.

Phylogenomic databases

eggNOGi COG1226.
GeneTreei ENSGT00760000118846.
HOGENOMi HOG000231015.
HOVERGENi HBG052230.
InParanoidi P19024.
KOi K04878.
OMAi GGELQCP.
OrthoDBi EOG7M0NRD.
PhylomeDBi P19024.
TreeFami TF313103.

Enzyme and pathway databases

Reactomei REACT_199159. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi 606769.
PROi P19024.

Gene expression databases

Genevestigatori P19024.

Family and domain databases

Gene3Di 1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProi IPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004052. K_chnl_volt-dep_Kv1.5.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view ]
PANTHERi PTHR11537. PTHR11537. 1 hit.
PTHR11537:SF25. PTHR11537:SF25. 1 hit.
Pfami PF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR00169. KCHANNEL.
PR01512. KV15CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTi SM00225. BTB. 1 hit.
[Graphical view ]
SUPFAMi SSF54695. SSF54695. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain."
    Swanson R., Marshall J., Smith J., Williams J., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.M.
    Neuron 4:929-939(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain.
  2. "Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart."
    Roberds S.L., Tamkun M.M.
    Proc. Natl. Acad. Sci. U.S.A. 88:1798-1802(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Heart.
  3. "The transcription of a mammalian voltage-gated potassium channel is regulated by cAMP in a cell-specific manner."
    Mori Y., Matsubara H., Folco E., Siegel A., Koren G.
    J. Biol. Chem. 268:26482-26493(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
  4. "SAP97 interacts with Kv1.5 in heterologous expression systems."
    Murata M., Buckett P.D., Zhou J., Brunner M., Folco E., Koren G.
    Am. J. Physiol. 281:H2575-H2584(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1, MUTAGENESIS OF 600-THR--LEU-602.
  5. "Caveolin-3 and SAP97 form a scaffolding protein complex that regulates the voltage-gated potassium channel Kv1.5."
    Folco E.J., Liu G.-X., Koren G.
    Am. J. Physiol. 287:H681-H690(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1 AND CAV3, FUNCTION IN CELL EXCITATION.

Entry informationi

Entry nameiKCNA5_RAT
AccessioniPrimary (citable) accession number: P19024
Secondary accession number(s): Q6LEB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 29, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3