Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium voltage-gated channel subfamily A member 5

Gene

Kcna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:2361015, PubMed:15618540). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:15618540). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (PubMed:15618540). Homotetrameric channels display rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).By similarity3 Publications

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: UniProtKB
  2. outward rectifier potassium channel activity Source: RGD
  3. potassium channel inhibitor activity Source: Ensembl
  4. receptor binding Source: RGD
  5. voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Source: Ensembl

GO - Biological processi

  1. membrane hyperpolarization Source: Ensembl
  2. negative regulation of cytosolic calcium ion concentration Source: RGD
  3. negative regulation of potassium ion transport Source: Ensembl
  4. positive regulation of G1/S transition of mitotic cell cycle Source: RGD
  5. positive regulation of myoblast proliferation Source: RGD
  6. potassium ion export Source: Ensembl
  7. potassium ion homeostasis Source: RGD
  8. potassium ion transmembrane transport Source: UniProtKB
  9. potassium ion transport Source: RGD
  10. protein homooligomerization Source: InterPro
  11. protein oligomerization Source: RGD
  12. regulation of atrial cardiac muscle cell membrane repolarization Source: Ensembl
  13. regulation of heart rate by cardiac conduction Source: Ensembl
  14. regulation of membrane potential Source: RGD
  15. regulation of vasoconstriction Source: Ensembl
  16. response to hydrogen peroxide Source: RGD
  17. response to hyperoxia Source: RGD
  18. response to hypoxia Source: RGD
  19. response to mechanical stimulus Source: RGD
  20. response to organic substance Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiREACT_352543. Voltage gated Potassium channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 5
Alternative name(s):
RCK7
RK4
Voltage-gated potassium channel subunit Kv1.5
Gene namesi
Name:Kcna5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2953. Kcna5.

Subcellular locationi

Cell membrane 2 Publications; Multi-pass membrane protein Curated

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 238238CytoplasmicBy similarityAdd
BLAST
Transmembranei239 – 26022Helical; Name=Segment S1By similarityAdd
BLAST
Topological domaini261 – 31454ExtracellularBy similarityAdd
BLAST
Transmembranei315 – 33622Helical; Name=Segment S2By similarityAdd
BLAST
Topological domaini337 – 34711CytoplasmicBy similarityAdd
BLAST
Transmembranei348 – 36821Helical; Name=Segment S3By similarityAdd
BLAST
Topological domaini369 – 38416ExtracellularBy similarityAdd
BLAST
Transmembranei385 – 40521Helical; Voltage-sensor; Name=Segment S4By similarityAdd
BLAST
Topological domaini406 – 42015CytoplasmicBy similarityAdd
BLAST
Transmembranei421 – 44222Helical; Name=Segment S5By similarityAdd
BLAST
Topological domaini443 – 45614ExtracellularBy similarityAdd
BLAST
Intramembranei457 – 46812Helical; Name=Pore helixBy similarityAdd
BLAST
Intramembranei469 – 4768By similarity
Topological domaini477 – 4837ExtracellularBy similarity
Transmembranei484 – 51229Helical; Name=Segment S6By similarityAdd
BLAST
Topological domaini513 – 60290CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: RGD
  2. Golgi apparatus Source: RGD
  3. integral component of membrane Source: GO_Central
  4. integral component of plasma membrane Source: UniProtKB
  5. intercalated disc Source: RGD
  6. intracellular canaliculus Source: RGD
  7. membrane raft Source: Ensembl
  8. perinuclear region of cytoplasm Source: RGD
  9. plasma membrane Source: RGD
  10. potassium channel complex Source: UniProtKB
  11. voltage-gated potassium channel complex Source: UniProtKB
  12. Z disc Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi600 – 6023TDL → AAA: Loss of interaction with DLG1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Potassium voltage-gated channel subfamily A member 5PRO_0000053989Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei81 – 811Phosphoserine; by CK2 and PKASequence Analysis
Cross-linki212 – 212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Lipidationi337 – 3371S-palmitoyl cysteineSequence Analysis
Cross-linki525 – 525Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei535 – 5351Phosphoserine; by PKASequence Analysis
Modified residuei546 – 5461Phosphoserine; by PKASequence Analysis
Modified residuei569 – 5691Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP19024.
PRIDEiP19024.

Expressioni

Tissue specificityi

Expressed equally in atrium, ventricle, aorta and skeletal muscle. Weaker expression in brain.1 Publication

Inductioni

Expression regulated by cAMP in a tissue-specific manner. In primary cardiac cells, levels increase by 6-fold, and in GH3 cells, levels decrease 5-6-fold.

Gene expression databases

GenevestigatoriP19024.

Interactioni

Subunit structurei

Homotetramer and heterotetramer of potassium channel proteins. Interacts with DLG1, which enhances channel currents (PubMed:11709425). Forms a ternary complex with DLG1 and CAV3 (PubMed:15277200). Interacts with KCNAB1 (By similarity). Interacts with UBE2I (By similarity).By similarity2 Publications

Protein-protein interaction databases

BioGridi247503. 4 interactions.
IntActiP19024. 2 interactions.
MINTiMINT-3973915.
STRINGi10116.ENSRNOP00000026691.

Structurei

3D structure databases

ProteinModelPortaliP19024.
SMRiP19024. Positions 111-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni407 – 42014S4-S5 linkerBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi469 – 4746Selectivity filterBy similarity
Motifi600 – 6023PDZ-binding

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi373 – 3764Poly-Gly

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP19024.
KOiK04878.
OMAiGGELQCP.
OrthoDBiEOG7M0NRD.
PhylomeDBiP19024.
TreeFamiTF313103.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004052. K_chnl_volt-dep_Kv1.5.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PTHR11537:SF25. PTHR11537:SF25. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01512. KV15CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.

Sequencei

Sequence statusi: Complete.

P19024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEISLVPLEN GSAMTLRGGG EAGASCVQTP RGECGCPPTS GLNNQSKETL
60 70 80 90 100
LRGRTTLEDA NQGGRPLPPM AQELPQPRRL SAEDEEGEGD PGLGTVEEDQ
110 120 130 140 150
APQDAGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP AKRLHYFDPL
160 170 180 190 200
RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA
210 220 230 240 250
MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV
260 270 280 290 300
ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGIN GSVSGALSSG
310 320 330 340 350
PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI
360 370 380 390 400
IDVVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF
410 420 430 440 450
KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNHG
460 470 480 490 500
SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP
510 520 530 540 550
VPVIVSNFNY FYHRETDHEE QAALKEEQGN QRRESGLDTG GQRKVSCSKA
560 570 580 590 600
SFCKTGGSLE SSDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET

DL
Length:602
Mass (Da):66,553
Last modified:November 1, 1990 - v1
Checksum:i6A784535FF226ED7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti553 – 5531C → S no nucleotide entry (PubMed:1705709).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27158 Genomic DNA. Translation: AAA41498.1.
L23434 Genomic DNA. Translation: AAA42337.1.
PIRiJH0166.
RefSeqiNP_037104.1. NM_012972.1.
UniGeneiRn.162789.

Genome annotation databases

EnsembliENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719.
GeneIDi25470.
KEGGirno:25470.
UCSCiRGD:2953. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27158 Genomic DNA. Translation: AAA41498.1.
L23434 Genomic DNA. Translation: AAA42337.1.
PIRiJH0166.
RefSeqiNP_037104.1. NM_012972.1.
UniGeneiRn.162789.

3D structure databases

ProteinModelPortaliP19024.
SMRiP19024. Positions 111-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247503. 4 interactions.
IntActiP19024. 2 interactions.
MINTiMINT-3973915.
STRINGi10116.ENSRNOP00000026691.

Proteomic databases

PaxDbiP19024.
PRIDEiP19024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719.
GeneIDi25470.
KEGGirno:25470.
UCSCiRGD:2953. rat.

Organism-specific databases

CTDi3741.
RGDi2953. Kcna5.

Phylogenomic databases

eggNOGiCOG1226.
GeneTreeiENSGT00760000118846.
HOGENOMiHOG000231015.
HOVERGENiHBG052230.
InParanoidiP19024.
KOiK04878.
OMAiGGELQCP.
OrthoDBiEOG7M0NRD.
PhylomeDBiP19024.
TreeFamiTF313103.

Enzyme and pathway databases

ReactomeiREACT_352543. Voltage gated Potassium channels.

Miscellaneous databases

NextBioi606769.
PROiP19024.

Gene expression databases

GenevestigatoriP19024.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004052. K_chnl_volt-dep_Kv1.5.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 1 hit.
PTHR11537:SF25. PTHR11537:SF25. 1 hit.
PfamiPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01512. KV15CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTiSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain."
    Swanson R., Marshall J., Smith J., Williams J., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.M.
    Neuron 4:929-939(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart."
    Roberds S.L., Tamkun M.M.
    Proc. Natl. Acad. Sci. U.S.A. 88:1798-1802(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Heart.
  3. "The transcription of a mammalian voltage-gated potassium channel is regulated by cAMP in a cell-specific manner."
    Mori Y., Matsubara H., Folco E., Siegel A., Koren G.
    J. Biol. Chem. 268:26482-26493(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
  4. "SAP97 interacts with Kv1.5 in heterologous expression systems."
    Murata M., Buckett P.D., Zhou J., Brunner M., Folco E., Koren G.
    Am. J. Physiol. 281:H2575-H2584(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1, MUTAGENESIS OF 600-THR--LEU-602.
  5. "Caveolin-3 and SAP97 form a scaffolding protein complex that regulates the voltage-gated potassium channel Kv1.5."
    Folco E.J., Liu G.-X., Koren G.
    Am. J. Physiol. 287:H681-H690(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DLG1 AND CAV3, FUNCTION IN CELL EXCITATION.
  6. "Heteromultimeric Kv1 channels contribute to myogenic control of arterial diameter."
    Plane F., Johnson R., Kerr P., Wiehler W., Thorneloe K., Ishii K., Chen T., Cole W.
    Circ. Res. 96:216-224(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKCNA5_RAT
AccessioniPrimary (citable) accession number: P19024
Secondary accession number(s): Q6LEB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: April 1, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.