P19024 (KCNA5_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily A member 5

Alternative name(s):
RCK7
RK4
Voltage-gated potassium channel subunit Kv1.5

Gene names

Name:

Kcna5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. This channel displays rapid activation and slow inactivation. Ref.5
Subunit structure	Heterotetramer of potassium channel proteins By similarity. Interacts with DLG1, which enhances channel currents. Forms a ternary complex with DLG1 and CAV3. Interacts with UBE2I By similarity. Ref.4 Ref.5
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed equally in atrium, ventricle, aorta and skeletal muscle. Weaker expression in brain. Ref.2
Induction	Expression regulated by cAMP in a tissue-specific manner. In primary cardiac cells, levels increase by 6-fold, and in GH3 cells, levels decrease 5-6-fold.
Domain	The N-terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Sequence similarities	Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.5/KCNA5 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Isopeptide bond Lipoprotein Palmitate Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	membrane hyperpolarization Inferred from electronic annotation. Source: Compara negative regulation of potassium ion transport Inferred from electronic annotation. Source: Compara potassium ion export Inferred from electronic annotation. Source: Compara potassium ion homeostasis Inferred from mutant phenotype PubMed 12127166. Source: RGD protein homooligomerization Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from mutant phenotype PubMed 12127166. Source: RGD reduction of cytosolic calcium ion concentration Inferred from mutant phenotype PubMed 15151918. Source: RGD regulation of atrial cardiac muscle cell membrane repolarization Inferred from electronic annotation. Source: Compara regulation of heart rate by cardiac conduction Inferred from electronic annotation. Source: Compara regulation of vasoconstriction Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from expression pattern PubMed 15151918. Source: RGD
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara intercalated disc Inferred from electronic annotation. Source: Compara membrane raft Inferred from electronic annotation. Source: Compara voltage-gated potassium channel complex Inferred from electronic annotation. Source: Compara
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome outward rectifier potassium channel activity Inferred from mutant phenotype PubMed 12127166PubMed 15930148. Source: RGD potassium channel inhibitor activity Inferred from electronic annotation. Source: Compara voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Potassium voltage-gated channel subfamily A member 5		PRO_0000053989
Regions
	Transmembrane	242 – 260	19	Helical; Name=Segment S1; Potential
	Transmembrane	316 – 336	21	Helical; Name=Segment S2; Potential
	Transmembrane	347 – 368	22	Helical; Name=Segment S3; Potential
	Transmembrane	387 – 408	22	Helical; Voltage-sensor; Name=Segment S4; Potential
	Transmembrane	423 – 444	22	Helical; Name=Segment S5; Potential
	Transmembrane	484 – 505	22	Helical; Name=Segment S6; Potential
	Motif	469 – 474	6	Selectivity filter By similarity
	Motif	600 – 602	3	PDZ-binding
	Compositional bias	373 – 376	4	Poly-Gly
Amino acid modifications
	Modified residue	81	1	Phosphoserine; by CK2 and PKA Potential
	Modified residue	535	1	Phosphoserine; by PKA Potential
	Modified residue	546	1	Phosphoserine; by PKA Potential
	Modified residue	569	1	Phosphoserine; by PKA Potential
	Lipidation	337	1	S-palmitoyl cysteine Potential
	Glycosylation	10	1	N-linked (GlcNAc...) Potential
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Cross-link	212		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	525		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Experimental info
	Mutagenesis	600 – 602	3	TDL → AAA: Loss of interaction with DLG1. Ref.4
	Sequence conflict	553	1	C → S no nucleotide entry Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P19024 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 6A784535FF226ED7	FASTA	602	66,553
	10 20 30 40 50 60 MEISLVPLEN GSAMTLRGGG EAGASCVQTP RGECGCPPTS GLNNQSKETL LRGRTTLEDA 70 80 90 100 110 120 NQGGRPLPPM AQELPQPRRL SAEDEEGEGD PGLGTVEEDQ APQDAGSLHH QRVLINISGL 130 140 150 160 170 180 RFETQLGTLA QFPNTLLGDP AKRLHYFDPL RNEYFFDRNR PSFDGILYYY QSGGRLRRPV 190 200 210 220 230 240 NVSLDVFADE IRFYQLGDEA MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA 250 260 270 280 290 300 RAIAIVSVLV ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGIN GSVSGALSSG 310 320 330 340 350 360 PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI IDVVAIFPYF 370 380 390 400 410 420 ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF KLSRHSKGLQ ILGKTLQASM 430 440 450 460 470 480 RELGLLIFFL FIGVILFSSA VYFAEADNHG SHFSSIPDAF WWAVVTMTTV GYGDMRPITV 490 500 510 520 530 540 GGKIVGSLCA IAGVLTIALP VPVIVSNFNY FYHRETDHEE QAALKEEQGN QRRESGLDTG 550 560 570 580 590 600 GQRKVSCSKA SFCKTGGSLE SSDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET DL

References

[1]	"Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain." Swanson R., Marshall J., Smith J., Williams J., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.M. Neuron 4:929-939(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain.
[2]	"Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart." Roberds S.L., Tamkun M.M. Proc. Natl. Acad. Sci. U.S.A. 88:1798-1802(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Heart.
[3]	"The transcription of a mammalian voltage-gated potassium channel is regulated by cAMP in a cell-specific manner." Mori Y., Matsubara H., Folco E., Siegel A., Koren G. J. Biol. Chem. 268:26482-26493(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
[4]	"SAP97 interacts with Kv1.5 in heterologous expression systems." Murata M., Buckett P.D., Zhou J., Brunner M., Folco E., Koren G. Am. J. Physiol. 281:H2575-H2584(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DLG1, MUTAGENESIS OF 600-THR--LEU-602.
[5]	"Caveolin-3 and SAP97 form a scaffolding protein complex that regulates the voltage-gated potassium channel Kv1.5." Folco E.J., Liu G.-X., Koren G. Am. J. Physiol. 287:H681-H690(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH DLG1 AND CAV3, FUNCTION IN CELL EXCITATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27158 Genomic DNA. Translation: AAA41498.1. L23434 Genomic DNA. Translation: AAA42337.1.
IPI	IPI00191629.
PIR	JH0166.
RefSeq	NP_037104.1. NM_012972.1.
UniGene	Rn.162789.
3D structure databases
ProteinModelPortal	P19024.
SMR	P19024. Positions 111-516.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3973915.
STRING	10116.ENSRNOP00000026691.
Proteomic databases
PaxDb	P19024.
PRIDE	P19024.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719.
GeneID	25470.
KEGG	rno:25470.
UCSC	RGD:2953. rat.
Organism-specific databases
CTD	3741.
RGD	2953. Kcna5.
Phylogenomic databases
eggNOG	COG1226.
GeneTree	ENSGT00560000076957.
HOGENOM	HOG000231015.
HOVERGEN	HBG052230.
InParanoid	P19024.
KO	K04878.
OMA	KRLRYFD.
OrthoDB	EOG4R502V.
Gene expression databases
Genevestigator	P19024.
GermOnline	ENSRNOG00000019719. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.120.350. 1 hit. 3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR027359. K_channel_four-helix_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003972. K_chnl_volt-dep_Kv1. IPR004052. K_chnl_volt-dep_Kv1.5. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit. PTHR11537:SF25. PTHR11537:SF25. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01512. KV15CHANNEL. PR01491. KVCHANNEL. PR01496. SHAKERCHANEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	606769.

Entry information

Entry name

KCNA5_RAT

Accession

Primary (citable) accession number: P19024
Secondary accession number(s): Q6LEB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	May 29, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program