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Protein

Potassium voltage-gated channel subfamily A member 5

Gene

Kcna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:2361015, PubMed:15618540). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:15618540). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (PubMed:15618540). Homotetrameric channels display rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

  • membrane hyperpolarization Source: Ensembl
  • membrane repolarization during atrial cardiac muscle cell action potential Source: Ensembl
  • negative regulation of cytosolic calcium ion concentration Source: RGD
  • negative regulation of potassium ion transport Source: Ensembl
  • Notch signaling pathway Source: Ensembl
  • positive regulation of G1/S transition of mitotic cell cycle Source: RGD
  • positive regulation of myoblast proliferation Source: RGD
  • potassium ion export Source: Ensembl
  • potassium ion homeostasis Source: RGD
  • potassium ion transmembrane transport Source: UniProtKB
  • potassium ion transport Source: RGD
  • protein complex oligomerization Source: RGD
  • protein homooligomerization Source: InterPro
  • regulation of atrial cardiac muscle cell membrane repolarization Source: Ensembl
  • regulation of heart rate by cardiac conduction Source: Ensembl
  • regulation of membrane potential Source: RGD
  • regulation of vasoconstriction Source: Ensembl
  • response to hydrogen peroxide Source: RGD
  • response to hyperoxia Source: RGD
  • response to hypoxia Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to organic substance Source: RGD

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

ReactomeiR-RNO-1296072 Voltage gated Potassium channels

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily A member 5
Alternative name(s):
RCK7
RK4
Voltage-gated potassium channel subunit Kv1.5
Gene namesi
Name:Kcna5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi2953 Kcna5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 238CytoplasmicBy similarityAdd BLAST238
Transmembranei239 – 260Helical; Name=Segment S1By similarityAdd BLAST22
Topological domaini261 – 314ExtracellularBy similarityAdd BLAST54
Transmembranei315 – 336Helical; Name=Segment S2By similarityAdd BLAST22
Topological domaini337 – 347CytoplasmicBy similarityAdd BLAST11
Transmembranei348 – 368Helical; Name=Segment S3By similarityAdd BLAST21
Topological domaini369 – 384ExtracellularBy similarityAdd BLAST16
Transmembranei385 – 405Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST21
Topological domaini406 – 420CytoplasmicBy similarityAdd BLAST15
Transmembranei421 – 442Helical; Name=Segment S5By similarityAdd BLAST22
Topological domaini443 – 456ExtracellularBy similarityAdd BLAST14
Intramembranei457 – 468Helical; Name=Pore helixBy similarityAdd BLAST12
Intramembranei469 – 476By similarity8
Topological domaini477 – 483ExtracellularBy similarity7
Transmembranei484 – 512Helical; Name=Segment S6By similarityAdd BLAST29
Topological domaini513 – 602CytoplasmicBy similarityAdd BLAST90

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi600 – 602TDL → AAA: Loss of interaction with DLG1. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539891 – 602Potassium voltage-gated channel subfamily A member 5Add BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei81Phosphoserine; by CK2 and PKASequence analysis1
Cross-linki212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Lipidationi337S-palmitoyl cysteineSequence analysis1
Cross-linki525Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei535Phosphoserine; by PKASequence analysis1
Modified residuei546Phosphoserine; by PKASequence analysis1
Modified residuei569Phosphoserine; by PKASequence analysis1

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP19024
PRIDEiP19024

Expressioni

Tissue specificityi

Expressed equally in atrium, ventricle, aorta and skeletal muscle. Weaker expression in brain.1 Publication

Inductioni

Expression regulated by cAMP in a tissue-specific manner. In primary cardiac cells, levels increase by 6-fold, and in GH3 cells, levels decrease 5-6-fold.

Gene expression databases

BgeeiENSRNOG00000019719
GenevisibleiP19024 RN

Interactioni

Subunit structurei

Homotetramer and heterotetramer of potassium channel proteins. Interacts with DLG1, which enhances channel currents (PubMed:11709425). Forms a ternary complex with DLG1 and CAV3 (PubMed:15277200). Interacts with KCNAB1 (By similarity). Interacts with UBE2I (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi247503, 6 interactors
CORUMiP19024
IntActiP19024, 4 interactors
MINTiP19024
STRINGi10116.ENSRNOP00000026691

Structurei

3D structure databases

ProteinModelPortaliP19024
SMRiP19024
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni407 – 420S4-S5 linkerBy similarityAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi469 – 474Selectivity filterBy similarity6
Motifi600 – 602PDZ-binding3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi373 – 376Poly-Gly4

Domaini

The N-terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1545 Eukaryota
COG1226 LUCA
GeneTreeiENSGT00760000118846
HOGENOMiHOG000231015
HOVERGENiHBG052230
InParanoidiP19024
KOiK04878
OMAiLPRNEFQ
OrthoDBiEOG091G10NU
PhylomeDBiP19024
TreeFamiTF313103

Family and domain databases

Gene3Di1.20.120.350, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR005821 Ion_trans_dom
IPR003968 K_chnl_volt-dep_Kv
IPR003972 K_chnl_volt-dep_Kv1
IPR004052 K_chnl_volt-dep_Kv1.5
IPR011333 SKP1/BTB/POZ_sf
IPR003131 T1-type_BTB
IPR028325 VG_K_chnl
IPR027359 Volt_channel_dom_sf
PANTHERiPTHR11537 PTHR11537, 1 hit
PfamiView protein in Pfam
PF02214 BTB_2, 1 hit
PF00520 Ion_trans, 1 hit
PRINTSiPR00169 KCHANNEL
PR01512 KV15CHANNEL
PR01491 KVCHANNEL
PR01496 SHAKERCHANEL
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SUPFAMiSSF54695 SSF54695, 1 hit

Sequencei

Sequence statusi: Complete.

P19024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEISLVPLEN GSAMTLRGGG EAGASCVQTP RGECGCPPTS GLNNQSKETL
60 70 80 90 100
LRGRTTLEDA NQGGRPLPPM AQELPQPRRL SAEDEEGEGD PGLGTVEEDQ
110 120 130 140 150
APQDAGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP AKRLHYFDPL
160 170 180 190 200
RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA
210 220 230 240 250
MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV
260 270 280 290 300
ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGIN GSVSGALSSG
310 320 330 340 350
PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI
360 370 380 390 400
IDVVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF
410 420 430 440 450
KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNHG
460 470 480 490 500
SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP
510 520 530 540 550
VPVIVSNFNY FYHRETDHEE QAALKEEQGN QRRESGLDTG GQRKVSCSKA
560 570 580 590 600
SFCKTGGSLE SSDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET

DL
Length:602
Mass (Da):66,553
Last modified:November 1, 1990 - v1
Checksum:i6A784535FF226ED7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti553C → S no nucleotide entry (PubMed:1705709).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27158 Genomic DNA Translation: AAA41498.1
L23434 Genomic DNA Translation: AAA42337.1
PIRiJH0166
RefSeqiNP_037104.1, NM_012972.1
UniGeneiRn.162789

Genome annotation databases

EnsembliENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719
GeneIDi25470
KEGGirno:25470
UCSCiRGD:2953 rat

Entry informationi

Entry nameiKCNA5_RAT
AccessioniPrimary (citable) accession number: P19024
Secondary accession number(s): Q6LEB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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