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P19024

- KCNA5_RAT

UniProt

P19024 - KCNA5_RAT

Protein

Potassium voltage-gated channel subfamily A member 5

Gene

Kcna5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1990)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. This channel displays rapid activation and slow inactivation.1 Publication

    GO - Molecular functioni

    1. delayed rectifier potassium channel activity Source: RefGenome
    2. outward rectifier potassium channel activity Source: RGD
    3. potassium channel inhibitor activity Source: Ensembl
    4. protein binding Source: RGD
    5. receptor binding Source: RGD
    6. voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Source: Ensembl

    GO - Biological processi

    1. membrane hyperpolarization Source: Ensembl
    2. negative regulation of cytosolic calcium ion concentration Source: RGD
    3. negative regulation of potassium ion transport Source: Ensembl
    4. positive regulation of G1/S transition of mitotic cell cycle Source: RGD
    5. positive regulation of myoblast proliferation Source: RGD
    6. potassium ion export Source: Ensembl
    7. potassium ion homeostasis Source: RGD
    8. potassium ion transport Source: RGD
    9. protein homooligomerization Source: InterPro
    10. protein oligomerization Source: RGD
    11. regulation of atrial cardiac muscle cell membrane repolarization Source: Ensembl
    12. regulation of heart rate by cardiac conduction Source: Ensembl
    13. regulation of membrane potential Source: RGD
    14. regulation of vasoconstriction Source: Ensembl
    15. response to hypoxia Source: RGD

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_199159. Voltage gated Potassium channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily A member 5
    Alternative name(s):
    RCK7
    RK4
    Voltage-gated potassium channel subunit Kv1.5
    Gene namesi
    Name:Kcna5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2953. Kcna5.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: RGD
    2. Golgi apparatus Source: RGD
    3. intercalated disc Source: RGD
    4. intracellular canaliculus Source: RGD
    5. membrane raft Source: Ensembl
    6. perinuclear region of cytoplasm Source: RGD
    7. plasma membrane Source: RGD
    8. voltage-gated potassium channel complex Source: Ensembl
    9. Z disc Source: RGD

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi600 – 6023TDL → AAA: Loss of interaction with DLG1. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 602602Potassium voltage-gated channel subfamily A member 5PRO_0000053989Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Modified residuei81 – 811Phosphoserine; by CK2 and PKASequence Analysis
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Cross-linki212 – 212Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Lipidationi337 – 3371S-palmitoyl cysteineSequence Analysis
    Cross-linki525 – 525Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei535 – 5351Phosphoserine; by PKASequence Analysis
    Modified residuei546 – 5461Phosphoserine; by PKASequence Analysis
    Modified residuei569 – 5691Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Glycoprotein, Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP19024.
    PRIDEiP19024.

    Expressioni

    Tissue specificityi

    Expressed equally in atrium, ventricle, aorta and skeletal muscle. Weaker expression in brain.1 Publication

    Inductioni

    Expression regulated by cAMP in a tissue-specific manner. In primary cardiac cells, levels increase by 6-fold, and in GH3 cells, levels decrease 5-6-fold.

    Gene expression databases

    GenevestigatoriP19024.

    Interactioni

    Subunit structurei

    Heterotetramer of potassium channel proteins By similarity. Interacts with DLG1, which enhances channel currents. Forms a ternary complex with DLG1 and CAV3. Interacts with UBE2I By similarity.By similarity

    Protein-protein interaction databases

    BioGridi247503. 4 interactions.
    IntActiP19024. 2 interactions.
    MINTiMINT-3973915.
    STRINGi10116.ENSRNOP00000026691.

    Structurei

    3D structure databases

    ProteinModelPortaliP19024.
    SMRiP19024. Positions 111-516.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei242 – 26019Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei316 – 33621Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei347 – 36822Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei387 – 40822Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei423 – 44422Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei484 – 50522Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi469 – 4746Selectivity filterBy similarity
    Motifi600 – 6023PDZ-binding

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi373 – 3764Poly-Gly

    Domaini

    The N-terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00740000115291.
    HOGENOMiHOG000231015.
    HOVERGENiHBG052230.
    InParanoidiP19024.
    KOiK04878.
    OMAiGGELQCP.
    OrthoDBiEOG7M0NRD.
    PhylomeDBiP19024.
    TreeFamiTF313103.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004052. K_chnl_volt-dep_Kv1.5.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view]
    PANTHERiPTHR11537. PTHR11537. 1 hit.
    PTHR11537:SF25. PTHR11537:SF25. 1 hit.
    PfamiPF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR00169. KCHANNEL.
    PR01512. KV15CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTiSM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P19024-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEISLVPLEN GSAMTLRGGG EAGASCVQTP RGECGCPPTS GLNNQSKETL    50
    LRGRTTLEDA NQGGRPLPPM AQELPQPRRL SAEDEEGEGD PGLGTVEEDQ 100
    APQDAGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP AKRLHYFDPL 150
    RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA 200
    MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV 250
    ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGIN GSVSGALSSG 300
    PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI 350
    IDVVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF 400
    KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNHG 450
    SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP 500
    VPVIVSNFNY FYHRETDHEE QAALKEEQGN QRRESGLDTG GQRKVSCSKA 550
    SFCKTGGSLE SSDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET 600
    DL 602
    Length:602
    Mass (Da):66,553
    Last modified:November 1, 1990 - v1
    Checksum:i6A784535FF226ED7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti553 – 5531C → S no nucleotide entry (PubMed:1705709)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27158 Genomic DNA. Translation: AAA41498.1.
    L23434 Genomic DNA. Translation: AAA42337.1.
    PIRiJH0166.
    RefSeqiNP_037104.1. NM_012972.1.
    UniGeneiRn.162789.

    Genome annotation databases

    EnsembliENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719.
    GeneIDi25470.
    KEGGirno:25470.
    UCSCiRGD:2953. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27158 Genomic DNA. Translation: AAA41498.1 .
    L23434 Genomic DNA. Translation: AAA42337.1 .
    PIRi JH0166.
    RefSeqi NP_037104.1. NM_012972.1.
    UniGenei Rn.162789.

    3D structure databases

    ProteinModelPortali P19024.
    SMRi P19024. Positions 111-516.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247503. 4 interactions.
    IntActi P19024. 2 interactions.
    MINTi MINT-3973915.
    STRINGi 10116.ENSRNOP00000026691.

    Proteomic databases

    PaxDbi P19024.
    PRIDEi P19024.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026691 ; ENSRNOP00000026691 ; ENSRNOG00000019719 .
    GeneIDi 25470.
    KEGGi rno:25470.
    UCSCi RGD:2953. rat.

    Organism-specific databases

    CTDi 3741.
    RGDi 2953. Kcna5.

    Phylogenomic databases

    eggNOGi COG1226.
    GeneTreei ENSGT00740000115291.
    HOGENOMi HOG000231015.
    HOVERGENi HBG052230.
    InParanoidi P19024.
    KOi K04878.
    OMAi GGELQCP.
    OrthoDBi EOG7M0NRD.
    PhylomeDBi P19024.
    TreeFami TF313103.

    Enzyme and pathway databases

    Reactomei REACT_199159. Voltage gated Potassium channels.

    Miscellaneous databases

    NextBioi 606769.
    PROi P19024.

    Gene expression databases

    Genevestigatori P19024.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR003091. K_chnl.
    IPR003968. K_chnl_volt-dep_Kv.
    IPR003972. K_chnl_volt-dep_Kv1.
    IPR004052. K_chnl_volt-dep_Kv1.5.
    IPR003131. T1-type_BTB.
    IPR028325. VG_K_chnl.
    [Graphical view ]
    PANTHERi PTHR11537. PTHR11537. 1 hit.
    PTHR11537:SF25. PTHR11537:SF25. 1 hit.
    Pfami PF02214. BTB_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR00169. KCHANNEL.
    PR01512. KV15CHANNEL.
    PR01491. KVCHANNEL.
    PR01496. SHAKERCHANEL.
    SMARTi SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain."
      Swanson R., Marshall J., Smith J., Williams J., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.M.
      Neuron 4:929-939(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Brain.
    2. "Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart."
      Roberds S.L., Tamkun M.M.
      Proc. Natl. Acad. Sci. U.S.A. 88:1798-1802(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Heart.
    3. "The transcription of a mammalian voltage-gated potassium channel is regulated by cAMP in a cell-specific manner."
      Mori Y., Matsubara H., Folco E., Siegel A., Koren G.
      J. Biol. Chem. 268:26482-26493(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
    4. "SAP97 interacts with Kv1.5 in heterologous expression systems."
      Murata M., Buckett P.D., Zhou J., Brunner M., Folco E., Koren G.
      Am. J. Physiol. 281:H2575-H2584(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG1, MUTAGENESIS OF 600-THR--LEU-602.
    5. "Caveolin-3 and SAP97 form a scaffolding protein complex that regulates the voltage-gated potassium channel Kv1.5."
      Folco E.J., Liu G.-X., Koren G.
      Am. J. Physiol. 287:H681-H690(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DLG1 AND CAV3, FUNCTION IN CELL EXCITATION.

    Entry informationi

    Entry nameiKCNA5_RAT
    AccessioniPrimary (citable) accession number: P19024
    Secondary accession number(s): Q6LEB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: November 1, 1990
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3