P19020 (DRD3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D(3) dopamine receptor

Alternative name(s):
Dopamine D3 receptor

Gene names

Name:

Drd3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation By similarity.
Subunit structure	Interacts with GRK4 and PALM By similarity. Interacts with CLIC6. Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Mainly in limbic areas of brain.
Post-translational modification	Phosphorylated by GRK4 By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor internalization Inferred from direct assay PubMed 14617818. Source: RGD acid secretion Inferred from direct assay PubMed 8302280. Source: BHF-UCL adenylate cyclase-activating dopamine receptor signaling pathway Inferred from electronic annotation. Source: Compara adenylate cyclase-inhibiting dopamine receptor signaling pathway Inferred from electronic annotation. Source: Compara arachidonic acid secretion Inferred from electronic annotation. Source: Compara behavioral response to cocaine Inferred from mutant phenotype PubMed 16026479. Source: RGD cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara circadian regulation of gene expression Inferred from electronic annotation. Source: Compara gastric emptying Inferred from mutant phenotype PubMed 18600456. Source: RGD locomotory behavior Inferred from electronic annotation. Source: Compara musculoskeletal movement, spinal reflex action Inferred from electronic annotation. Source: Compara negative regulation of adenylate cyclase activity Inferred from direct assay PubMed 8302280. Source: BHF-UCL negative regulation of blood pressure Inferred from electronic annotation. Source: Compara negative regulation of dopamine receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of oligodendrocyte differentiation Inferred from electronic annotation. Source: Compara negative regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara negative regulation of protein secretion Inferred from electronic annotation. Source: Compara negative regulation of sodium:hydrogen antiporter activity Inferred from mutant phenotype PubMed 15265811. Source: RGD negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16902178. Source: RGD positive regulation of cell proliferation Inferred from direct assay PubMed 15584906. Source: RGD positive regulation of dopamine receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of mitosis Inferred from mutant phenotype PubMed 8302280. Source: BHF-UCL positive regulation of renal sodium excretion Inferred from direct assay PubMed 14621190. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 16902178. Source: RGD prepulse inhibition Inferred from electronic annotation. Source: Compara regulation of blood volume by renin-angiotensin Inferred from electronic annotation. Source: Compara regulation of circadian sleep/wake cycle, sleep Inferred from mutant phenotype PubMed 15913875. Source: RGD regulation of dopamine secretion Inferred from electronic annotation. Source: Compara regulation of lipid metabolic process Inferred from electronic annotation. Source: Compara regulation of locomotion involved in locomotory behavior Inferred from mutant phenotype PubMed 22394078. Source: RGD regulation of multicellular organism growth Inferred from electronic annotation. Source: Compara response to amphetamine Inferred from electronic annotation. Source: Compara response to drug Inferred from direct assay Ref.1. Source: BHF-UCL response to ethanol Inferred from electronic annotation. Source: Compara response to histamine Inferred from electronic annotation. Source: Compara response to morphine Inferred from electronic annotation. Source: Compara synaptic transmission, dopaminergic Inferred from mutant phenotype PubMed 16490190. Source: RGD visual learning Inferred from electronic annotation. Source: Compara
Cellular_component	apical part of cell Inferred from direct assay PubMed 9822449. Source: RGD cell projection Inferred from direct assay PubMed 15584906. Source: RGD endocytic vesicle Inferred from direct assay PubMed 14617818. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.4. Source: RGD
Molecular_function	dopamine binding Inferred from mutant phenotype PubMed 16490190. Source: RGD dopamine neurotransmitter receptor activity, coupled via Gi/Go Inferred from direct assay Ref.1PubMed 8302280. Source: BHF-UCL drug binding Inferred from direct assay Ref.1. Source: BHF-UCL
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P19020-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform D3(TM3-del) (identifier: P19020-2)

The sequence of this isoform differs from the canonical sequence as follows:
     92-100: TGGVWNFSR → HSGGHASSL
     101-446: Missing.

Isoform D3(O2-DEL) (identifier: P19020-3)

The sequence of this isoform differs from the canonical sequence as follows:
     176-193: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	D(3) dopamine receptor		PRO_0000069400
Regions
	Topological domain	1 – 32	32	Extracellular By similarity
	Transmembrane	33 – 55	23	Helical; Name=1; By similarity
	Topological domain	56 – 65	10	Cytoplasmic By similarity
	Transmembrane	66 – 88	23	Helical; Name=2; By similarity
	Topological domain	89 – 104	16	Extracellular By similarity
	Transmembrane	105 – 126	22	Helical; Name=3; By similarity
	Topological domain	127 – 149	23	Cytoplasmic By similarity
	Transmembrane	150 – 170	21	Helical; Name=4; By similarity
	Topological domain	171 – 187	17	Extracellular By similarity
	Transmembrane	188 – 212	25	Helical; Name=5; By similarity
	Topological domain	213 – 375	163	Cytoplasmic By similarity
	Transmembrane	376 – 397	22	Helical; Name=6; By similarity
	Topological domain	398 – 412	15	Extracellular By similarity
	Transmembrane	413 – 434	22	Helical; Name=7; By similarity
	Topological domain	435 – 446	12	Cytoplasmic By similarity
	Region	189 – 196	8	Agonist binding By similarity
	Region	388 – 396	9	Agonist binding By similarity
Sites
	Binding site	110	1	Agonist By similarity
Amino acid modifications
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 181		By similarity
	Disulfide bond	401 ↔ 404		By similarity
Natural variations
	Alternative sequence	92 – 100	9	TGGVWNFSR → HSGGHASSL in isoform D3(TM3-del).		VSP_001874
	Alternative sequence	101 – 446	346	Missing in isoform D3(TM3-del).		VSP_001875
	Alternative sequence	176 – 193	18	Missing in isoform D3(O2-DEL).		VSP_001876
Experimental info
	Sequence conflict	71	1	L → M Ref.3
	Sequence conflict	139	1	Q → E in AAA41076. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: 180E36AFF217E9DB	FASTA	446	49,516
	10 20 30 40 50 60 MAPLSQISTH LNSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL 70 80 90 100 110 120 QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR ICCDVFVTLD VMMCTASILN 130 140 150 160 170 180 LCAISIDRYT AVVMPVHYQH GTGQSSCRRV ALMITAVWVL AFAVSCPLLF GFNTTGDPSI 190 200 210 220 230 240 CSISNPDFVI YSSVVSFYVP FGVTVLVYAR IYIVLRQRQR KRILTRQNSQ CISIRPGFPQ 250 260 270 280 290 300 QSSCLRLHPI RQFSIRARFL SDATGQMEHI EDKQYPQKCQ DPLLSHLQPP SPGQTHGGLK 310 320 330 340 350 360 RYYSICQDTA LRHPSLEGGA GMSPVERTRN SLSPTMAPKL SLEVRKLSNG RLSTSLRLGP 370 380 390 400 410 420 LQPRGVPLRE KKATQMVVIV LGAFIVCWLP FFLTHVLNTH CQACHVSPEL YRATTWLGYV 430 440 NSALNPVIYT TFNVEFRKAF LKILSC
	Isoform D3(TM3-del) [UniParc]. Checksum: 3FB0359132B0FE91	FASTA	100	10,602
	10 20 30 40 50 60 MAPLSQISTH LNSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL 70 80 90 100 QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VHSGGHASSL
	Isoform D3(O2-DEL) [UniParc]. Checksum: 0C0BCDE0150177DD	FASTA	428	47,633
	10 20 30 40 50 60 MAPLSQISTH LNSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV CAAVLRERAL 70 80 90 100 110 120 QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR ICCDVFVTLD VMMCTASILN 130 140 150 160 170 180 LCAISIDRYT AVVMPVHYQH GTGQSSCRRV ALMITAVWVL AFAVSCPLLF GFNTTVVSFY 190 200 210 220 230 240 VPFGVTVLVY ARIYIVLRQR QRKRILTRQN SQCISIRPGF PQQSSCLRLH PIRQFSIRAR 250 260 270 280 290 300 FLSDATGQME HIEDKQYPQK CQDPLLSHLQ PPSPGQTHGG LKRYYSICQD TALRHPSLEG 310 320 330 340 350 360 GAGMSPVERT RNSLSPTMAP KLSLEVRKLS NGRLSTSLRL GPLQPRGVPL REKKATQMVV 370 380 390 400 410 420 IVLGAFIVCW LPFFLTHVLN THCQACHVSP ELYRATTWLG YVNSALNPVI YTTFNVEFRK AFLKILSC

References

[1]	"Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics." Sokoloff P., Giros B., Martres M.-P., Bouthenet M.-L., Schwartz J.-C. Nature 347:146-151(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[2]	"Shorter variants of the D3 dopamine receptor produced through various patterns of alternative splicing." Giros B., Martres M.-P., Pilon C., Sokoloff P., Schwartz J.-C. Biochem. Biophys. Res. Commun. 176:1584-1592(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
[3]	"Characterization of a novel exon within the D3 receptor gene giving rise to an mRNA isoform expressed in rat brain." Pagliusi S., Chollet-Daemerius A., Losberger C., Mills A., Kawashima E. Biochem. Biophys. Res. Commun. 194:465-471(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-106.
[4]	"CLIC6, a member of the intracellular chloride channel family, interacts with dopamine D(2)-like receptors." Griffon N., Jeanneteau F., Prieur F., Diaz J., Sokoloff P. Brain Res. Mol. Brain Res. 117:47-57(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CLIC6.
[5]	"Molecular modelling of D2-like dopamine receptors." Livingstone C.D., Strange P.G., Naylor L.H. Biochem. J. 287:277-282(1992) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53944 mRNA. Translation: CAA37887.1. M69194 , M69189, M69190, M69191, M69192, M69193 Genomic DNA. Translation: AAA41076.1. Sequence problems. S63847, S63846 Genomic DNA. Translation: AAB27545.1.
IPI	IPI00191625. IPI00230790. IPI00230792.
PIR	DYRTD3. S11565.
RefSeq	NP_058836.1. NM_017140.1.
UniGene	Rn.10356.
3D structure databases
ProteinModelPortal	P19020.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000031550.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P19020.
Proteomic databases
PaxDb	P19020.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000029474; ENSRNOP00000031550; ENSRNOG00000026772.
GeneID	29238.
KEGG	rno:29238.
UCSC	RGD:2521. rat.
Organism-specific databases
CTD	1814.
RGD	2521. Drd3.
Phylogenomic databases
eggNOG	NOG309657.
GeneTree	ENSGT00700000104182.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	P19020.
KO	K04146.
OMA	EGRTRNS.
OrthoDB	EOG47M1Z5.
Gene expression databases
Genevestigator	P19020.
GermOnline	ENSRNOG00000026772. Rattus norvegicus.
Family and domain databases
InterPro	IPR001620. Dopa_D3_rcpt. IPR000929. Dopamine_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00568. DOPAMINED3R. PR00242. DOPAMINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P19020.
ChEMBL	CHEMBL3138.
NextBio	608506.

Entry information

Entry name

DRD3_RAT

Accession

Primary (citable) accession number: P19020
Secondary accession number(s): P70647

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program