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Protein

D(3) dopamine receptor

Gene

Drd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101AgonistBy similarity

GO - Molecular functioni

  • D1 dopamine receptor binding Source: RGD
  • dopamine binding Source: RGD
  • dopamine neurotransmitter receptor activity Source: RGD
  • dopamine neurotransmitter receptor activity, coupled via Gi/Go Source: BHF-UCL
  • drug binding Source: BHF-UCL
  • protein domain specific binding Source: RGD

GO - Biological processi

  • acid secretion Source: BHF-UCL
  • adenylate cyclase-activating dopamine receptor signaling pathway Source: Ensembl
  • adenylate cyclase-inhibiting dopamine receptor signaling pathway Source: Ensembl
  • arachidonic acid secretion Source: Ensembl
  • behavioral response to cocaine Source: RGD
  • cellular calcium ion homeostasis Source: Ensembl
  • circadian regulation of gene expression Source: Ensembl
  • dopamine receptor signaling pathway Source: RGD
  • gastric emptying Source: RGD
  • G-protein coupled receptor internalization Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • locomotory behavior Source: Ensembl
  • musculoskeletal movement, spinal reflex action Source: Ensembl
  • negative regulation of adenylate cyclase activity Source: BHF-UCL
  • negative regulation of blood pressure Source: Ensembl
  • negative regulation of dopamine receptor signaling pathway Source: Ensembl
  • negative regulation of oligodendrocyte differentiation Source: Ensembl
  • negative regulation of protein kinase B signaling Source: Ensembl
  • negative regulation of protein secretion Source: Ensembl
  • negative regulation of sodium:proton antiporter activity Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of cytokinesis Source: Ensembl
  • positive regulation of dopamine receptor signaling pathway Source: Ensembl
  • positive regulation of mitotic nuclear division Source: BHF-UCL
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • prepulse inhibition Source: Ensembl
  • regulation of blood volume by renin-angiotensin Source: Ensembl
  • regulation of cAMP metabolic process Source: BHF-UCL
  • regulation of circadian sleep/wake cycle, sleep Source: RGD
  • regulation of dopamine secretion Source: Ensembl
  • regulation of lipid metabolic process Source: Ensembl
  • regulation of locomotion Source: RGD
  • regulation of locomotion involved in locomotory behavior Source: RGD
  • regulation of multicellular organism growth Source: Ensembl
  • response to amphetamine Source: Ensembl
  • response to drug Source: BHF-UCL
  • response to ethanol Source: Ensembl
  • response to histamine Source: Ensembl
  • response to morphine Source: Ensembl
  • synaptic transmission, dopaminergic Source: RGD
  • visual learning Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-390651. Dopamine receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
D(3) dopamine receptor
Alternative name(s):
Dopamine D3 receptor
Gene namesi
Name:Drd3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi2521. Drd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232ExtracellularBy similarityAdd
BLAST
Transmembranei33 – 5523Helical; Name=1By similarityAdd
BLAST
Topological domaini56 – 6510CytoplasmicBy similarity
Transmembranei66 – 8823Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10416ExtracellularBy similarityAdd
BLAST
Transmembranei105 – 12622Helical; Name=3By similarityAdd
BLAST
Topological domaini127 – 14923CytoplasmicBy similarityAdd
BLAST
Transmembranei150 – 17021Helical; Name=4By similarityAdd
BLAST
Topological domaini171 – 18717ExtracellularBy similarityAdd
BLAST
Transmembranei188 – 21225Helical; Name=5By similarityAdd
BLAST
Topological domaini213 – 375163CytoplasmicBy similarityAdd
BLAST
Transmembranei376 – 39722Helical; Name=6By similarityAdd
BLAST
Topological domaini398 – 41215ExtracellularBy similarityAdd
BLAST
Transmembranei413 – 43422Helical; Name=7By similarityAdd
BLAST
Topological domaini435 – 44612CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • apical part of cell Source: RGD
  • cell projection Source: RGD
  • endocytic vesicle Source: RGD
  • integral component of plasma membrane Source: InterPro
  • membrane Source: BHF-UCL
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2093868.
GuidetoPHARMACOLOGYi216.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446D(3) dopamine receptorPRO_0000069400Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi12 – 121N-linked (GlcNAc...)Sequence analysis
Glycosylationi19 – 191N-linked (GlcNAc...)Sequence analysis
Glycosylationi97 – 971N-linked (GlcNAc...)Sequence analysis
Disulfide bondi103 ↔ 181PROSITE-ProRule annotation
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence analysis
Disulfide bondi401 ↔ 404PROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by GRK4.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP19020.

PTM databases

PhosphoSiteiP19020.

Expressioni

Tissue specificityi

Mainly in limbic areas of brain.

Gene expression databases

GenevisibleiP19020. RN.

Interactioni

Subunit structurei

Interacts with CLIC6 (PubMed:14499480). Interacts with GRK4. Interacts with PALM. Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA (By similarity).By similarity1 Publication

GO - Molecular functioni

  • D1 dopamine receptor binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031550.

Chemistry

BindingDBiP19020.

Structurei

3D structure databases

ProteinModelPortaliP19020.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1968Agonist bindingBy similarity
Regioni388 – 3969Agonist bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP19020.
KOiK04146.
OMAiQKCQDPL.
OrthoDBiEOG769ZMG.
PhylomeDBiP19020.
TreeFamiTF334382.

Family and domain databases

InterProiIPR001620. Dopamine_D3_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF38. PTHR24249:SF38. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00568. DOPAMINED3R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P19020-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPLSQISTH LNSTCGAENS TGVNRARPHA YYALSYCALI LAIIFGNGLV
60 70 80 90 100
CAAVLRERAL QTTTNYLVVS LAVADLLVAT LVMPWVVYLE VTGGVWNFSR
110 120 130 140 150
ICCDVFVTLD VMMCTASILN LCAISIDRYT AVVMPVHYQH GTGQSSCRRV
160 170 180 190 200
ALMITAVWVL AFAVSCPLLF GFNTTGDPSI CSISNPDFVI YSSVVSFYVP
210 220 230 240 250
FGVTVLVYAR IYIVLRQRQR KRILTRQNSQ CISIRPGFPQ QSSCLRLHPI
260 270 280 290 300
RQFSIRARFL SDATGQMEHI EDKQYPQKCQ DPLLSHLQPP SPGQTHGGLK
310 320 330 340 350
RYYSICQDTA LRHPSLEGGA GMSPVERTRN SLSPTMAPKL SLEVRKLSNG
360 370 380 390 400
RLSTSLRLGP LQPRGVPLRE KKATQMVVIV LGAFIVCWLP FFLTHVLNTH
410 420 430 440
CQACHVSPEL YRATTWLGYV NSALNPVIYT TFNVEFRKAF LKILSC
Length:446
Mass (Da):49,516
Last modified:November 1, 1990 - v1
Checksum:i180E36AFF217E9DB
GO
Isoform D3(TM3-del) (identifier: P19020-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     92-100: TGGVWNFSR → HSGGHASSL
     101-446: Missing.

Show »
Length:100
Mass (Da):10,602
Checksum:i3FB0359132B0FE91
GO
Isoform D3(O2-DEL) (identifier: P19020-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-193: Missing.

Show »
Length:428
Mass (Da):47,633
Checksum:i0C0BCDE0150177DD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711L → M (PubMed:8333859).Curated
Sequence conflicti139 – 1391Q → E in AAA41076 (PubMed:2039532).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei92 – 1009TGGVWNFSR → HSGGHASSL in isoform D3(TM3-del). CuratedVSP_001874
Alternative sequencei101 – 446346Missing in isoform D3(TM3-del). CuratedVSP_001875Add
BLAST
Alternative sequencei176 – 19318Missing in isoform D3(O2-DEL). CuratedVSP_001876Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53944 mRNA. Translation: CAA37887.1.
M69194
, M69189, M69190, M69191, M69192, M69193 Genomic DNA. Translation: AAA41076.1. Sequence problems.
S63847, S63846 Genomic DNA. Translation: AAB27545.1.
PIRiS11565. DYRTD3.
RefSeqiNP_058836.1. NM_017140.2. [P19020-1]
UniGeneiRn.10356.

Genome annotation databases

EnsembliENSRNOT00000089057; ENSRNOP00000073117; ENSRNOG00000060806. [P19020-1]
GeneIDi29238.
KEGGirno:29238.
UCSCiRGD:2521. rat. [P19020-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53944 mRNA. Translation: CAA37887.1.
M69194
, M69189, M69190, M69191, M69192, M69193 Genomic DNA. Translation: AAA41076.1. Sequence problems.
S63847, S63846 Genomic DNA. Translation: AAB27545.1.
PIRiS11565. DYRTD3.
RefSeqiNP_058836.1. NM_017140.2. [P19020-1]
UniGeneiRn.10356.

3D structure databases

ProteinModelPortaliP19020.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031550.

Chemistry

BindingDBiP19020.
ChEMBLiCHEMBL2093868.
GuidetoPHARMACOLOGYi216.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP19020.

Proteomic databases

PaxDbiP19020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000089057; ENSRNOP00000073117; ENSRNOG00000060806. [P19020-1]
GeneIDi29238.
KEGGirno:29238.
UCSCiRGD:2521. rat. [P19020-1]

Organism-specific databases

CTDi1814.
RGDi2521. Drd3.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP19020.
KOiK04146.
OMAiQKCQDPL.
OrthoDBiEOG769ZMG.
PhylomeDBiP19020.
TreeFamiTF334382.

Enzyme and pathway databases

ReactomeiR-RNO-390651. Dopamine receptors.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

NextBioi608506.
PROiP19020.

Gene expression databases

GenevisibleiP19020. RN.

Family and domain databases

InterProiIPR001620. Dopamine_D3_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF38. PTHR24249:SF38. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00568. DOPAMINED3R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics."
    Sokoloff P., Giros B., Martres M.-P., Bouthenet M.-L., Schwartz J.-C.
    Nature 347:146-151(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  2. "Shorter variants of the D3 dopamine receptor produced through various patterns of alternative splicing."
    Giros B., Martres M.-P., Pilon C., Sokoloff P., Schwartz J.-C.
    Biochem. Biophys. Res. Commun. 176:1584-1592(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
  3. "Characterization of a novel exon within the D3 receptor gene giving rise to an mRNA isoform expressed in rat brain."
    Pagliusi S., Chollet-Daemerius A., Losberger C., Mills A., Kawashima E.
    Biochem. Biophys. Res. Commun. 194:465-471(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-106.
  4. "CLIC6, a member of the intracellular chloride channel family, interacts with dopamine D(2)-like receptors."
    Griffon N., Jeanneteau F., Prieur F., Diaz J., Sokoloff P.
    Brain Res. Mol. Brain Res. 117:47-57(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CLIC6.
  5. "Molecular modelling of D2-like dopamine receptors."
    Livingstone C.D., Strange P.G., Naylor L.H.
    Biochem. J. 287:277-282(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.
    Tissue: Brain.

Entry informationi

Entry nameiDRD3_RAT
AccessioniPrimary (citable) accession number: P19020
Secondary accession number(s): P70647
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 11, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.