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Protein

Keratin, type I cytoskeletal 15

Gene

KRT15

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. scaffold protein binding Source: BHF-UCL
  2. structural constituent of cytoskeleton Source: ProtInc

GO - Biological processi

  1. epidermis development Source: ProtInc
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 15
Alternative name(s):
Cytokeratin-15
Short name:
CK-15
Keratin-15
Short name:
K15
Gene namesi
Name:KRT15
Synonyms:KRTB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:6421. KRT15.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProtKB
  2. intermediate filament Source: ProtInc
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30208.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Keratin, type I cytoskeletal 15PRO_0000063657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei301 – 3011PhosphothreonineBy similarity
Modified residuei323 – 3231PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP19012.
PaxDbiP19012.
PeptideAtlasiP19012.
PRIDEiP19012.

PTM databases

PhosphoSiteiP19012.

Expressioni

Tissue specificityi

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.2 Publications

Gene expression databases

BgeeiP19012.
CleanExiHS_KRT15.
ExpressionAtlasiP19012. baseline and differential.
GenevestigatoriP19012.

Organism-specific databases

HPAiCAB000135.
HPA023910.
HPA024554.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins.

Binary interactionsi

WithEntry#Exp.IntActNotes
RCOR3Q9P2K32EBI-739566,EBI-743428
taxP140793EBI-739566,EBI-9675698From a different organism.
USP2O756042EBI-739566,EBI-743272

Protein-protein interaction databases

BioGridi110064. 118 interactions.
IntActiP19012. 56 interactions.
MINTiMINT-1433992.
STRINGi9606.ENSP00000254043.

Structurei

3D structure databases

ProteinModelPortaliP19012.
SMRiP19012. Positions 156-254, 270-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 104104HeadAdd
BLAST
Regioni105 – 413309RodAdd
BLAST
Regioni105 – 14036Coil 1AAdd
BLAST
Regioni141 – 15919Linker 1Add
BLAST
Regioni160 – 25192Coil 1BAdd
BLAST
Regioni252 – 27120Linker 12Add
BLAST
Regioni272 – 413142Coil 2Add
BLAST
Regioni414 – 45643TailAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi14 – 10390Gly-richAdd
BLAST
Compositional biasi428 – 4314Poly-Gly

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG148784.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP19012.
KOiK07604.
OMAiFHINVEE.
PhylomeDBiP19012.
TreeFamiTF332742.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
IPR009053. Prefoldin.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SUPFAMiSSF46579. SSF46579. 1 hit.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P19012-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTTFLQTSS STFGGGSTRG GSLLAGGGGF GGGSLSGGGG SRSISASSAR
60 70 80 90 100
FVSSGSGGGY GGGMRVCGFG GGAGSVFGGG FGGGVGGGFG GGFGGGDGGL
110 120 130 140 150
LSGNEKITMQ NLNDRLASYL DKVRALEEAN ADLEVKIHDW YQKQTPTSPE
160 170 180 190 200
CDYSQYFKTI EELRDKIMAT TIDNSRVILE IDNARLAADD FRLKYENELA
210 220 230 240 250
LRQGVEADIN GLRRVLDELT LARTDLEMQI EGLNEELAYL KKNHEEEMKE
260 270 280 290 300
FSSQLAGQVN VEMDAAPGVD LTRVLAEMRE QYEAMAEKNR RDVEAWFFSK
310 320 330 340 350
TEELNKEVAS NTEMIQTSKT EITDLRRTMQ ELEIELQSQL SMKAGLENSL
360 370 380 390 400
AETECRYATQ LQQIQGLIGG LEAQLSELRC EMEAQNQEYK MLLDIKTRLE
410 420 430 440 450
QEIATYRSLL EGQDAKMAGI AIREASSGGG GSSSNFHINV EESVDGQVVS

SHKREI
Length:456
Mass (Da):49,212
Last modified:November 2, 2010 - v3
Checksum:i812C1343905EBE41
GO
Isoform 2 (identifier: P19012-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     3-29: TTFLQTSSSTFGGGSTRGGSLLAGGGG → GTRSRPQPAQNATTANTSRPLKSSGTR
     30-194: Missing.
     417-456: Missing.

Note: No experimental confirmation available.

Show »
Length:251
Mass (Da):28,602
Checksum:i0EED60B048471B9B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti147 – 1471T → A.2 Publications
Corresponds to variant rs1050784 [ dbSNP | Ensembl ].
VAR_047429
Natural varianti416 – 4161K → R.1 Publication
Corresponds to variant rs2305556 [ dbSNP | Ensembl ].
VAR_047430
Natural varianti421 – 4211A → G.6 Publications
Corresponds to variant rs897420 [ dbSNP | Ensembl ].
VAR_047431

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei3 – 2927TTFLQ…AGGGG → GTRSRPQPAQNATTANTSRP LKSSGTR in isoform 2. 1 PublicationVSP_056507Add
BLAST
Alternative sequencei30 – 194165Missing in isoform 2. 1 PublicationVSP_056508Add
BLAST
Alternative sequencei417 – 45640Missing in isoform 2. 1 PublicationVSP_056509Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07696 mRNA. Translation: CAA30535.1.
AF202320 Genomic DNA. Translation: AAF27047.1.
BT007261 mRNA. Translation: AAP35925.1.
AK090604 mRNA. Translation: BAG52193.1.
AK091239 mRNA. Translation: BAG52314.1.
AC019349 Genomic DNA. No translation available.
BC002641 mRNA. Translation: AAH02641.1.
CCDSiCCDS11398.1. [P19012-1]
PIRiS01069. KRHU5.
RefSeqiNP_002266.2. NM_002275.3.
XP_005257402.1. XM_005257345.1. [P19012-1]
UniGeneiHs.654570.

Genome annotation databases

EnsembliENST00000254043; ENSP00000254043; ENSG00000171346. [P19012-1]
ENST00000393976; ENSP00000377546; ENSG00000171346. [P19012-1]
GeneIDi3866.
KEGGihsa:3866.
UCSCiuc002hwy.3. human. [P19012-1]

Polymorphism databases

DMDMi311033438.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07696 mRNA. Translation: CAA30535.1.
AF202320 Genomic DNA. Translation: AAF27047.1.
BT007261 mRNA. Translation: AAP35925.1.
AK090604 mRNA. Translation: BAG52193.1.
AK091239 mRNA. Translation: BAG52314.1.
AC019349 Genomic DNA. No translation available.
BC002641 mRNA. Translation: AAH02641.1.
CCDSiCCDS11398.1. [P19012-1]
PIRiS01069. KRHU5.
RefSeqiNP_002266.2. NM_002275.3.
XP_005257402.1. XM_005257345.1. [P19012-1]
UniGeneiHs.654570.

3D structure databases

ProteinModelPortaliP19012.
SMRiP19012. Positions 156-254, 270-412.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110064. 118 interactions.
IntActiP19012. 56 interactions.
MINTiMINT-1433992.
STRINGi9606.ENSP00000254043.

PTM databases

PhosphoSiteiP19012.

Polymorphism databases

DMDMi311033438.

Proteomic databases

MaxQBiP19012.
PaxDbiP19012.
PeptideAtlasiP19012.
PRIDEiP19012.

Protocols and materials databases

DNASUi3866.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254043; ENSP00000254043; ENSG00000171346. [P19012-1]
ENST00000393976; ENSP00000377546; ENSG00000171346. [P19012-1]
GeneIDi3866.
KEGGihsa:3866.
UCSCiuc002hwy.3. human. [P19012-1]

Organism-specific databases

CTDi3866.
GeneCardsiGC17M039675.
HGNCiHGNC:6421. KRT15.
HPAiCAB000135.
HPA023910.
HPA024554.
MIMi148030. gene.
neXtProtiNX_P19012.
PharmGKBiPA30208.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG148784.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP19012.
KOiK07604.
OMAiFHINVEE.
PhylomeDBiP19012.
TreeFamiTF332742.

Miscellaneous databases

ChiTaRSiKRT15. human.
GeneWikiiKeratin_15.
GenomeRNAii3866.
NextBioi15201.
PROiP19012.
SOURCEiSearch...

Gene expression databases

BgeeiP19012.
CleanExiHS_KRT15.
ExpressionAtlasiP19012. baseline and differential.
GenevestigatoriP19012.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
IPR009053. Prefoldin.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SUPFAMiSSF46579. SSF46579. 1 hit.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cells derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene."
    Bader B.L., Jahn L., Franke W.W.
    Eur. J. Cell Biol. 47:300-319(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-147 AND GLY-421.
  2. "Molecular characterization and expression of the stratification-related cytokeratins 4 and 15."
    Leube R.E., Bader B.L., Bosch F.X., Zimbelmann R., Achtstaetter T., Franke W.W.
    J. Cell Biol. 106:1249-1261(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANTS ALA-147 AND GLY-421.
  3. "Genomic organization and amplification of the human keratin 15 and 19 genes."
    Whittock N.V., Eady R.A.J., McGrath J.A.
    Biochem. Biophys. Res. Commun. 267:462-465(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-421.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS ARG-416 AND GLY-421.
    Tissue: Tongue.
  6. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLY-421.
    Tissue: Pancreas.
  8. "Keratin 15 expression in stratified epithelia: downregulation in activated keratinocytes."
    Waseem A., Dogan B., Tidman N., Alam Y., Purkis P., Jackson S., Lalli A., Machesney M., Leigh I.M.
    J. Invest. Dermatol. 112:362-369(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] GLY-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiK1C15_HUMAN
AccessioniPrimary (citable) accession number: P19012
Secondary accession number(s): B3KQY1
, B3KRA2, E0CX14, Q53XV8, Q9BUG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 2, 2010
Last modified: February 4, 2015
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.