Reviewed, UniProtKB/Swiss-Prot P19000 (PA21B_LATLA)

Names and origin

Protein names	Recommended name:     Phospholipase A2 P'513     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase
Organism	Laticauda laticaudata (Blue-ringed sea krait)
Taxonomic identifier	8630 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Laticaudinae › Laticauda

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	In contrast to the pancreatic enzymes, the molecules of venom phospholipases A2 are dimers of identical chains; the monomers appear to be inactive.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 27	6			PRO_0000022886
	Chain	28 – 145	118	Phospholipase A2 P'513		PRO_0000022887
Sites
	Active site	75	1	By similarity
	Active site	119	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 98		By similarity
	Disulfide bond	54 ↔ 144		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 125		By similarity
	Disulfide bond	78 ↔ 118		By similarity
	Disulfide bond	87 ↔ 111		By similarity
	Disulfide bond	105 ↔ 116		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P19000-1 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: C6C2A9B4EE653630	FASTA	145	16,242
	10 20 30 40 50 60 MYPAHLLLLL AVCVSLLGAS AIPPLPLNLA QFALVIKCAD KGKRPRWHYM DYGCYCGPGG 70 80 90 100 110 120 SGTPVDELDR CCKTHDQCYA QAEKKGCYPK LTMYSYYCGG DGPYCNSKTE CQRFVCDCDV 130 140 RAADCFARYP YNNKNYNINT SKRCK

References

[1]	"Sequence of a cDNA encoding a snake venom phospholipase A2." Guignery Frelat G., Ducancel F., Menez A., Boulain J.-C. Nucleic Acids Res. 15:5892-5892(1987) [PubMed: 3615209] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00377 mRNA. Translation: CAA68449.1.
PIR	A27099.
3D structure databases
HSSP	HSSP built from PDB template 1AE7 based on UniProtKB P00608.
SMR	P19000. Positions 28-145.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P19000.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
ProDom	PD000303. PhospholipaseA2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_LATLA

Accession

Primary (citable) accession number: P19000

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)