P18965 (VSPG_DABSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Factor V activator RVV-V gamma EC=3.4.21.95 Alternative name(s): Russel's viper venom FV activator gamma Short name=RVV-V gamma Snake venom serine protease Short name=SVSP
Organism	Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Taxonomic identifier	343250 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Viperinae › Daboia › Daboia russellii

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Venom serine protease that selectively activates factor V (F5) in a calcium-independent manner. It cleaves the Arg(1545)-Ser(1546) linkage in the human factor V molecule. Induces the coagulation of mammalian plasma. Ref.1
Catalytic activity	Fully activates human clotting factor V by a single cleavage at the 1545-Trp-Tyr-Leu-Arg-|-Ser-Asn-Asn-Gly-1552 bond. Cattle, but not rabbit, factor V is cleaved, and no other proteins of the clotting system are attacked. Esterase activity is observed on Bz-Arg-OEt and Tos-Arg-OMe, and amidase activity on Phe-pipecolyl-Arg-NHPhNO2.
Subunit structure	Monomer.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Miscellaneous	There are three isoproteins of RVV-V, designated RVV-V alpha, V-beta, and V-gamma.
Sequence similarities	Belongs to the peptidase S1 family. Snake venom subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Blood coagulation cascade activating toxin Hemostasis impairing toxin Hydrolase Protease Serine protease Toxin
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 236

236

Factor V activator RVV-V gamma

PRO_0000088739

Regions

Domain

1 – 227

227

Peptidase S1

Sites

Active site

43

1

Charge relay system Ref.3

Active site

88

1

Charge relay system Ref.3

Active site

182

1

Charge relay system Ref.3

Amino acid modifications

Glycosylation

229

1

N-linked (GlcNAc...) Ref.1

Disulfide bond

7 ↔ 141

Ref.3

Disulfide bond

28 ↔ 44

Ref.3

Disulfide bond

76 ↔ 234

Ref.3

Disulfide bond

120 ↔ 188

Ref.3

Disulfide bond

152 ↔ 167

Ref.3

Disulfide bond

178 ↔ 203

Ref.3

Secondary structure

1

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236

Helix Strand Turn

Details...

Beta strand

15 – 20

6

Beta strand

23 – 34

12

Beta strand

37 – 40

4

Helix

42 – 44

3

Beta strand

49 – 54

6

Beta strand

56 – 60

5

Beta strand

66 – 68

3

Beta strand

70 – 74

5

Helix

85 – 87

3

Beta strand

90 – 96

7

Beta strand

119 – 126

8

Beta strand

128 – 131

4

Beta strand

140 – 147

8

Helix

149 – 151

3

Turn

152 – 155

4

Beta strand

163 – 169

7

Beta strand

185 – 188

4

Beta strand

191 – 198

8

Beta strand

210 – 214

5

Helix

215 – 218

4

Helix

219 – 227

9

Sequences

	Sequence		Length	Mass (Da)
	P18965 [UniParc]. Last modified November 1, 1990. Version 1. Checksum: ED7C01F17DB8F7C5	FASTA	236	26,167
	10 20 30 40 50 60 VVGGDECNIN EHPFLVALYT SASSTIHCAG ALINREWVLT AAHCDRRNIR IKLGMHSKNI 70 80 90 100 110 120 RNEDEQIRVP RGKYFCLNTK FPNGLDKDIM LIRLRRPVTY STHIAPVSLP SRSRGVGSRC 130 140 150 160 170 180 RIMGWGKIST TEDTYPDVPH CTNIFIVKHK WCEPLYPWVP ADSRTLCAGI LKGGRDTCHG 190 200 210 220 230 DSGGPLICNG EMHGIVAGGS EPCGQHLKPA VYTKVFDYNN WIQSIIAGNR TVTCPP

References

[1]	"The factor V-activating enzyme (RVV-V) from Russell's viper venom. Identification of isoproteins RVV-V alpha, -V beta, and -V gamma and their complete amino acid sequences." Tokunaga F., Nagasawa K., Tamura S., Miyata T., Iwanaga S., Kisiel W. J. Biol. Chem. 263:17471-17481(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, GLYCOSYLATION AT ASN-229. Tissue: Venom.
[2]	"Crystallization and preliminary X-ray crystallographic analysis of blood coagulation factor V-activating proteinase (RVV-V) from Russell's viper venom." Nakayama D., Ben Ammar Y., Takeda S. Acta Crystallogr. F 65:1306-1308(2009) [PubMed] [Europe PMC] [Abstract] Cited for: CRYSTALLIZATION.
[3]	"Structural basis of coagulation factor V recognition for cleavage by RVV-V." Nakayama D., Ben Ammar Y., Miyata T., Takeda S. FEBS Lett. 585:3020-3025(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), ACTIVE SITE, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

B32121.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3S9A	X-ray	1.90	A	1-236	[»]
3S9B	X-ray	1.90	A	1-236	[»]
3S9C	X-ray	1.80	A	1-236	[»]
3SBK	X-ray	2.55	A	1-236	[»]

ProteinModelPortal

P18965.

SMR

P18965. Positions 1-236.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

HOVERGEN

HBG013304.

Family and domain databases

InterPro

IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P18965.

Entry information

Entry name

VSPG_DABSI

Accession

Primary (citable) accession number: P18965

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	November 1, 1990
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Annotation program

Animal Toxin Annotation Program