ID   IRA1_YEAST              Reviewed;        3092 AA.
AC   P18963; D6VQD6;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   27-MAR-2024, entry version 189.
DE   RecName: Full=Inhibitory regulator protein IRA1;
GN   Name=IRA1; Synonyms=GLC1, PPD1; OrderedLocusNames=YBR140C;
GN   ORFNames=YBR1016;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2767.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7754712; DOI=10.1002/yea.320100911;
RA   Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A.,
RA   Slonimski P.P., Sokolowska B., Herbert C.J.;
RT   "The sequence of 12.5 kb from the right arm of chromosome II predicts a new
RT   N-terminal sequence for the IRA1 protein and reveals two new genes, one of
RT   which is a DEAD-box helicase.";
RL   Yeast 10:1227-1234(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-3092.
RX   PubMed=2540426; DOI=10.1128/mcb.9.2.757-768.1989;
RA   Tanaka K., Matsumoto K., Toh-e A.;
RT   "IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in
RT   Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 9:757-768(1989).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2768-3092.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091856; DOI=10.1002/yea.320100002;
RA   Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F.,
RA   Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M.,
RA   Herbert C.J.;
RT   "The sequence of 29.7 kb from the right arm of chromosome II reveals 13
RT   complete open reading frames, of which ten correspond to new genes.";
RL   Yeast 10:S1-S11(1994).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-915 AND SER-1342,
RP   AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Inhibitory regulator of the Ras-cyclic AMP pathway in
CC       S.cerevisiae. Stimulates the GTPase activity of Ras proteins.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
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DR   EMBL; Z36009; CAA85098.1; -; Genomic_DNA.
DR   EMBL; M24378; AAA34709.1; -; Genomic_DNA.
DR   EMBL; X75891; CAA53498.1; -; Genomic_DNA.
DR   EMBL; X78937; CAA55537.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07256.1; -; Genomic_DNA.
DR   PIR; S46009; S46009.
DR   RefSeq; NP_009698.3; NM_001178488.3.
DR   SMR; P18963; -.
DR   BioGRID; 32840; 68.
DR   DIP; DIP-2318N; -.
DR   IntAct; P18963; 5.
DR   MINT; P18963; -.
DR   STRING; 4932.YBR140C; -.
DR   iPTMnet; P18963; -.
DR   MaxQB; P18963; -.
DR   PaxDb; 4932-YBR140C; -.
DR   PeptideAtlas; P18963; -.
DR   EnsemblFungi; YBR140C_mRNA; YBR140C; YBR140C.
DR   GeneID; 852437; -.
DR   KEGG; sce:YBR140C; -.
DR   AGR; SGD:S000000344; -.
DR   SGD; S000000344; IRA1.
DR   VEuPathDB; FungiDB:YBR140C; -.
DR   eggNOG; KOG1826; Eukaryota.
DR   GeneTree; ENSGT00940000176574; -.
DR   HOGENOM; CLU_000439_0_0_1; -.
DR   InParanoid; P18963; -.
DR   OMA; CPANDID; -.
DR   OrthoDB; 1356198at2759; -.
DR   BioCyc; YEAST:G3O-29094-MONOMER; -.
DR   BioGRID-ORCS; 852437; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P18963; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P18963; Protein.
DR   GO; GO:0016020; C:membrane; IMP:SGD.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0005096; F:GTPase activator activity; IMP:SGD.
DR   GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IMP:SGD.
DR   GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:SGD.
DR   CDD; cd05392; RasGAP_Neurofibromin_like; 1.
DR   Gene3D; 3.40.525.10; CRAL-TRIO lipid binding domain; 1.
DR   InterPro; IPR036865; CRAL-TRIO_dom_sf.
DR   InterPro; IPR039360; Ras_GTPase.
DR   InterPro; IPR023152; RasGAP_CS.
DR   InterPro; IPR001936; RasGAP_dom.
DR   InterPro; IPR008936; Rho_GTPase_activation_prot.
DR   PANTHER; PTHR10194:SF150; INHIBITORY REGULATOR PROTEIN IRA1-RELATED; 1.
DR   PANTHER; PTHR10194; RAS GTPASE-ACTIVATING PROTEINS; 1.
DR   Pfam; PF00616; RasGAP; 2.
DR   SMART; SM00323; RasGAP; 1.
DR   SUPFAM; SSF48350; GTPase activation domain, GAP; 1.
DR   PROSITE; PS00509; RAS_GTPASE_ACTIV_1; 1.
DR   PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; GTPase activation; Phosphoprotein; Reference proteome.
FT   CHAIN           1..3092
FT                   /note="Inhibitory regulator protein IRA1"
FT                   /id="PRO_0000056657"
FT   DOMAIN          1709..1898
FT                   /note="Ras-GAP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
FT   REGION          375..430
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          450..487
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          946..988
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1003..1023
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        380..430
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        951..988
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         497
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         915
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         1342
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         1753
FT                   /note="Phosphoserine; by PKA"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         3004
FT                   /note="Phosphoserine; by PKA"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        361
FT                   /note="V -> G (in Ref. 4; AAA34709)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   3092 AA;  350980 MW;  291339AE5BD26E08 CRC64;
     MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN SCRSIILNIA
     ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV LLRLLSDVFE YYWDQNNDFK
     KIRNDNYKPG FSSHRPNFHT SRPKHTRINP ALATMLLCKI SKLKFNTRTL KVLQNMSHHL
     SGSATISKSS ILPDSQEFLQ KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA
     PLLISHTSTE LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA
     FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL LFDDVYSTFS
     VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK QSSVNASGNV SPSQFSTGND
     ASPTSPMASL SSPLNTNILG YPLSPITSTL GQANTSTSTT AATTKTDADT PSTMNTNNNN
     NNNNSANLNN IPQRIFSLDD ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG
     VNNSQSQNDQ SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY
     RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK FLTYLIRNLN
     GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS KRFYNLLGQN LEVGTNWNSA
     TANTFISHCV ERNPLTHRRL QLEFFASGLQ LDSDLFLRHL QLEKELNHID LPKISLYTEG
     FRVFFHLVST KKLHEDIAEK TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL
     DQFDAARTLS NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR
     TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT TKRYASPNDS
     ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA TPTILKNIKS PKPNKTKKIA
     DDKQLKQPSY SRVILSDNDE ARKIMMNIFS IFKRMTNWFI RPDANTEFPK TFTDIIKPLF
     VSILDSNQRL QVTARAFIEI PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD
     LKLENAKREM LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS
     LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD NFTASGSVAL
     QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT QEELVDFRSL AGILASMSGI
     LSDMQELEKS KSAPDNEGDS LSFESRNPAY EVHKSLKLEL TKKMNFFISK QCQWLNNPNL
     LTRENSRDIL SIELHPLSFN LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI
     LKRDDDEKIM LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG
     ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY IDTSIESAKA
     LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL EKSADLNQFP VSLRHKISIL
     NENVIIALTN LSNANVNVSL KFTLPMGYSP NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK
     MLAIDDFLKY IIKNPILAFF GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK
     RAARSDDILR RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN
     SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY IAVGSFVFLR
     FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR ENIFKKDILV SKEEFLKTCS
     DKIFNFLSEL CKIPTNNFTV NVREDPTPIS FDYSFLHKFF YLNEFTIRKE IINESKLPGE
     FSFLKNTVML NDKILGVLGQ PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE
     EEDNAPFVHE AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT
     TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV ENPYLVTTIP
     RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD KEKKKFCPVS LKIGNKYFQV
     LHEIPQLYKV TVSNRTFSIK FNNVYKISNL ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP
     KYLEIVKMFY YAQLKMEEDF GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV
     GLFNEDDLVK NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST
     ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG PEAISRIIYS
     LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH ALDRDSENRD WMKAVSILTS
     FPTTEIACQV IEKLINMIKS FLPSLAVEAS AHSWSELTIL SKISVSIFFE SPLLSQMYLP
     EILFAVSLLI DVGPSEIRVS LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF
     ISGFSQEKGR VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA
     IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM FIIIAHVFTY
     SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM VNCLKRLYTV HLQLGFDGKS
     LAKKLMESRN FAATLLAKLE SYNGCIWNED NFPHIILGFI ANGLSIPVVK GAALDCLQAL
     FKNTYYERKS NPKSSDYLCY LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI
     NWLSSDSDKS NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF
     RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV VKPLDIFDQE
     HIEKLKGEGE HQVAIYERKR LATMILARMS CS
//