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P18963

- IRA1_YEAST

UniProt

P18963 - IRA1_YEAST

Protein

Inhibitory regulator protein IRA1

Gene

IRA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Inhibitory regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae. Stimulates the GTPase activity of Ras proteins.

    GO - Molecular functioni

    1. Ras GTPase activator activity Source: SGD

    GO - Biological processi

    1. negative regulation of cAMP biosynthetic process Source: SGD
    2. negative regulation of Ras protein signal transduction Source: SGD
    3. positive regulation of Ras GTPase activity Source: SGD
    4. regulation of adenylate cyclase activity Source: SGD
    5. signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29094-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inhibitory regulator protein IRA1
    Gene namesi
    Name:IRA1
    Synonyms:GLC1, PPD1
    Ordered Locus Names:YBR140C
    ORF Names:YBR1016
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR140c.
    SGDiS000000344. IRA1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 30923092Inhibitory regulator protein IRA1PRO_0000056657Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei497 – 4971Phosphoserine2 Publications
    Modified residuei915 – 9151Phosphoserine1 Publication
    Modified residuei1342 – 13421Phosphoserine1 Publication
    Modified residuei1753 – 17531Phosphoserine; by PKASequence Analysis
    Modified residuei3004 – 30041Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP18963.
    PaxDbiP18963.

    Expressioni

    Gene expression databases

    GenevestigatoriP18963.

    Interactioni

    Protein-protein interaction databases

    BioGridi32840. 34 interactions.
    DIPiDIP-2318N.
    IntActiP18963. 3 interactions.
    MINTiMINT-688782.
    STRINGi4932.YBR140C.

    Structurei

    3D structure databases

    ProteinModelPortaliP18963.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1709 – 1898190Ras-GAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi326 – 3316Poly-Ser
    Compositional biasi379 – 3835Poly-Ser
    Compositional biasi475 – 48410Poly-Asn
    Compositional biasi1433 – 14375Poly-Ile
    Compositional biasi2039 – 20424Poly-Glu

    Sequence similaritiesi

    Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5261.
    GeneTreeiENSGT00550000074797.
    HOGENOMiHOG000113101.
    OMAiFCIIADI.
    OrthoDBiEOG7H1JTR.

    Family and domain databases

    Gene3Di1.10.506.10. 1 hit.
    InterProiIPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view]
    PfamiPF00616. RasGAP. 1 hit.
    [Graphical view]
    SMARTiSM00323. RasGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P18963-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN     50
    SCRSIILNIA ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV 100
    LLRLLSDVFE YYWDQNNDFK KIRNDNYKPG FSSHRPNFHT SRPKHTRINP 150
    ALATMLLCKI SKLKFNTRTL KVLQNMSHHL SGSATISKSS ILPDSQEFLQ 200
    KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA PLLISHTSTE 250
    LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA 300
    FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL 350
    LFDDVYSTFS VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK 400
    QSSVNASGNV SPSQFSTGND ASPTSPMASL SSPLNTNILG YPLSPITSTL 450
    GQANTSTSTT AATTKTDADT PSTMNTNNNN NNNNSANLNN IPQRIFSLDD 500
    ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG VNNSQSQNDQ 550
    SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY 600
    RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK 650
    FLTYLIRNLN GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS 700
    KRFYNLLGQN LEVGTNWNSA TANTFISHCV ERNPLTHRRL QLEFFASGLQ 750
    LDSDLFLRHL QLEKELNHID LPKISLYTEG FRVFFHLVST KKLHEDIAEK 800
    TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL DQFDAARTLS 850
    NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR 900
    TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT 950
    TKRYASPNDS ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA 1000
    TPTILKNIKS PKPNKTKKIA DDKQLKQPSY SRVILSDNDE ARKIMMNIFS 1050
    IFKRMTNWFI RPDANTEFPK TFTDIIKPLF VSILDSNQRL QVTARAFIEI 1100
    PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD LKLENAKREM 1150
    LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS 1200
    LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD 1250
    NFTASGSVAL QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT 1300
    QEELVDFRSL AGILASMSGI LSDMQELEKS KSAPDNEGDS LSFESRNPAY 1350
    EVHKSLKLEL TKKMNFFISK QCQWLNNPNL LTRENSRDIL SIELHPLSFN 1400
    LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI LKRDDDEKIM 1450
    LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG 1500
    ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY 1550
    IDTSIESAKA LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL 1600
    EKSADLNQFP VSLRHKISIL NENVIIALTN LSNANVNVSL KFTLPMGYSP 1650
    NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK MLAIDDFLKY IIKNPILAFF 1700
    GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK RAARSDDILR 1750
    RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN 1800
    SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY 1850
    IAVGSFVFLR FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR 1900
    ENIFKKDILV SKEEFLKTCS DKIFNFLSEL CKIPTNNFTV NVREDPTPIS 1950
    FDYSFLHKFF YLNEFTIRKE IINESKLPGE FSFLKNTVML NDKILGVLGQ 2000
    PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE EEDNAPFVHE 2050
    AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT 2100
    TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV 2150
    ENPYLVTTIP RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD 2200
    KEKKKFCPVS LKIGNKYFQV LHEIPQLYKV TVSNRTFSIK FNNVYKISNL 2250
    ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP KYLEIVKMFY YAQLKMEEDF 2300
    GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV GLFNEDDLVK 2350
    NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST 2400
    ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG 2450
    PEAISRIIYS LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH 2500
    ALDRDSENRD WMKAVSILTS FPTTEIACQV IEKLINMIKS FLPSLAVEAS 2550
    AHSWSELTIL SKISVSIFFE SPLLSQMYLP EILFAVSLLI DVGPSEIRVS 2600
    LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF ISGFSQEKGR 2650
    VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA 2700
    IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM 2750
    FIIIAHVFTY SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM 2800
    VNCLKRLYTV HLQLGFDGKS LAKKLMESRN FAATLLAKLE SYNGCIWNED 2850
    NFPHIILGFI ANGLSIPVVK GAALDCLQAL FKNTYYERKS NPKSSDYLCY 2900
    LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI NWLSSDSDKS 2950
    NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF 3000
    RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV 3050
    VKPLDIFDQE HIEKLKGEGE HQVAIYERKR LATMILARMS CS 3092
    Length:3,092
    Mass (Da):350,980
    Last modified:October 1, 1994 - v2
    Checksum:i291339AE5BD26E08
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti361 – 3611V → G in AAA34709. (PubMed:2540426)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36009 Genomic DNA. Translation: CAA85098.1.
    M24378 Genomic DNA. Translation: AAA34709.1.
    X75891 Genomic DNA. Translation: CAA53498.1.
    X78937 Genomic DNA. Translation: CAA55537.1.
    BK006936 Genomic DNA. Translation: DAA07256.1.
    PIRiS46009.
    RefSeqiNP_009698.3. NM_001178488.3.

    Genome annotation databases

    EnsemblFungiiYBR140C; YBR140C; YBR140C.
    GeneIDi852437.
    KEGGisce:YBR140C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36009 Genomic DNA. Translation: CAA85098.1 .
    M24378 Genomic DNA. Translation: AAA34709.1 .
    X75891 Genomic DNA. Translation: CAA53498.1 .
    X78937 Genomic DNA. Translation: CAA55537.1 .
    BK006936 Genomic DNA. Translation: DAA07256.1 .
    PIRi S46009.
    RefSeqi NP_009698.3. NM_001178488.3.

    3D structure databases

    ProteinModelPortali P18963.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32840. 34 interactions.
    DIPi DIP-2318N.
    IntActi P18963. 3 interactions.
    MINTi MINT-688782.
    STRINGi 4932.YBR140C.

    Proteomic databases

    MaxQBi P18963.
    PaxDbi P18963.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR140C ; YBR140C ; YBR140C .
    GeneIDi 852437.
    KEGGi sce:YBR140C.

    Organism-specific databases

    CYGDi YBR140c.
    SGDi S000000344. IRA1.

    Phylogenomic databases

    eggNOGi COG5261.
    GeneTreei ENSGT00550000074797.
    HOGENOMi HOG000113101.
    OMAi FCIIADI.
    OrthoDBi EOG7H1JTR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29094-MONOMER.

    Miscellaneous databases

    NextBioi 971330.

    Gene expression databases

    Genevestigatori P18963.

    Family and domain databases

    Gene3Di 1.10.506.10. 1 hit.
    InterProi IPR001936. RasGAP.
    IPR023152. RasGAP_CS.
    IPR008936. Rho_GTPase_activation_prot.
    [Graphical view ]
    Pfami PF00616. RasGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00323. RasGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
    PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "The sequence of 12.5 kb from the right arm of chromosome II predicts a new N-terminal sequence for the IRA1 protein and reveals two new genes, one of which is a DEAD-box helicase."
      Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A., Slonimski P.P., Sokolowska B., Herbert C.J.
      Yeast 10:1227-1234(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2767.
      Strain: ATCC 204508 / S288c.
    4. "IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae."
      Tanaka K., Matsumoto K., Toh-e A.
      Mol. Cell. Biol. 9:757-768(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-3092.
    5. "The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
      Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
      Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2768-3092.
      Strain: ATCC 204508 / S288c.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-915 AND SER-1342, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIRA1_YEAST
    AccessioniPrimary (citable) accession number: P18963
    Secondary accession number(s): D6VQD6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3