SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P18963

- IRA1_YEAST

UniProt

P18963 - IRA1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Inhibitory regulator protein IRA1
Gene
IRA1, GLC1, PPD1, YBR140C, YBR1016
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae. Stimulates the GTPase activity of Ras proteins.

GO - Molecular functioni

  1. Ras GTPase activator activity Source: SGD

GO - Biological processi

  1. negative regulation of Ras protein signal transduction Source: SGD
  2. negative regulation of cAMP biosynthetic process Source: SGD
  3. positive regulation of Ras GTPase activity Source: SGD
  4. regulation of adenylate cyclase activity Source: SGD
  5. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-29094-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory regulator protein IRA1
Gene namesi
Name:IRA1
Synonyms:GLC1, PPD1
Ordered Locus Names:YBR140C
ORF Names:YBR1016
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR140c.
SGDiS000000344. IRA1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30923092Inhibitory regulator protein IRA1
PRO_0000056657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei497 – 4971Phosphoserine2 Publications
Modified residuei915 – 9151Phosphoserine1 Publication
Modified residuei1342 – 13421Phosphoserine1 Publication
Modified residuei1753 – 17531Phosphoserine; by PKA Reviewed prediction
Modified residuei3004 – 30041Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18963.
PaxDbiP18963.

Expressioni

Gene expression databases

GenevestigatoriP18963.

Interactioni

Protein-protein interaction databases

BioGridi32840. 34 interactions.
DIPiDIP-2318N.
IntActiP18963. 3 interactions.
MINTiMINT-688782.
STRINGi4932.YBR140C.

Structurei

3D structure databases

ProteinModelPortaliP18963.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1709 – 1898190Ras-GAP
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi326 – 3316Poly-Ser
Compositional biasi379 – 3835Poly-Ser
Compositional biasi475 – 48410Poly-Asn
Compositional biasi1433 – 14375Poly-Ile
Compositional biasi2039 – 20424Poly-Glu

Sequence similaritiesi

Contains 1 Ras-GAP domain.

Phylogenomic databases

eggNOGiCOG5261.
GeneTreeiENSGT00550000074797.
HOGENOMiHOG000113101.
OMAiFCIIADI.
OrthoDBiEOG7H1JTR.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
InterProiIPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18963-1 [UniParc]FASTAAdd to Basket

« Hide

MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN     50
SCRSIILNIA ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV 100
LLRLLSDVFE YYWDQNNDFK KIRNDNYKPG FSSHRPNFHT SRPKHTRINP 150
ALATMLLCKI SKLKFNTRTL KVLQNMSHHL SGSATISKSS ILPDSQEFLQ 200
KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA PLLISHTSTE 250
LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA 300
FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL 350
LFDDVYSTFS VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK 400
QSSVNASGNV SPSQFSTGND ASPTSPMASL SSPLNTNILG YPLSPITSTL 450
GQANTSTSTT AATTKTDADT PSTMNTNNNN NNNNSANLNN IPQRIFSLDD 500
ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG VNNSQSQNDQ 550
SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY 600
RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK 650
FLTYLIRNLN GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS 700
KRFYNLLGQN LEVGTNWNSA TANTFISHCV ERNPLTHRRL QLEFFASGLQ 750
LDSDLFLRHL QLEKELNHID LPKISLYTEG FRVFFHLVST KKLHEDIAEK 800
TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL DQFDAARTLS 850
NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR 900
TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT 950
TKRYASPNDS ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA 1000
TPTILKNIKS PKPNKTKKIA DDKQLKQPSY SRVILSDNDE ARKIMMNIFS 1050
IFKRMTNWFI RPDANTEFPK TFTDIIKPLF VSILDSNQRL QVTARAFIEI 1100
PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD LKLENAKREM 1150
LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS 1200
LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD 1250
NFTASGSVAL QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT 1300
QEELVDFRSL AGILASMSGI LSDMQELEKS KSAPDNEGDS LSFESRNPAY 1350
EVHKSLKLEL TKKMNFFISK QCQWLNNPNL LTRENSRDIL SIELHPLSFN 1400
LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI LKRDDDEKIM 1450
LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG 1500
ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY 1550
IDTSIESAKA LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL 1600
EKSADLNQFP VSLRHKISIL NENVIIALTN LSNANVNVSL KFTLPMGYSP 1650
NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK MLAIDDFLKY IIKNPILAFF 1700
GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK RAARSDDILR 1750
RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN 1800
SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY 1850
IAVGSFVFLR FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR 1900
ENIFKKDILV SKEEFLKTCS DKIFNFLSEL CKIPTNNFTV NVREDPTPIS 1950
FDYSFLHKFF YLNEFTIRKE IINESKLPGE FSFLKNTVML NDKILGVLGQ 2000
PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE EEDNAPFVHE 2050
AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT 2100
TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV 2150
ENPYLVTTIP RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD 2200
KEKKKFCPVS LKIGNKYFQV LHEIPQLYKV TVSNRTFSIK FNNVYKISNL 2250
ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP KYLEIVKMFY YAQLKMEEDF 2300
GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV GLFNEDDLVK 2350
NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST 2400
ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG 2450
PEAISRIIYS LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH 2500
ALDRDSENRD WMKAVSILTS FPTTEIACQV IEKLINMIKS FLPSLAVEAS 2550
AHSWSELTIL SKISVSIFFE SPLLSQMYLP EILFAVSLLI DVGPSEIRVS 2600
LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF ISGFSQEKGR 2650
VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA 2700
IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM 2750
FIIIAHVFTY SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM 2800
VNCLKRLYTV HLQLGFDGKS LAKKLMESRN FAATLLAKLE SYNGCIWNED 2850
NFPHIILGFI ANGLSIPVVK GAALDCLQAL FKNTYYERKS NPKSSDYLCY 2900
LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI NWLSSDSDKS 2950
NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF 3000
RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV 3050
VKPLDIFDQE HIEKLKGEGE HQVAIYERKR LATMILARMS CS 3092
Length:3,092
Mass (Da):350,980
Last modified:October 1, 1994 - v2
Checksum:i291339AE5BD26E08
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 3611V → G in AAA34709. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36009 Genomic DNA. Translation: CAA85098.1.
M24378 Genomic DNA. Translation: AAA34709.1.
X75891 Genomic DNA. Translation: CAA53498.1.
X78937 Genomic DNA. Translation: CAA55537.1.
BK006936 Genomic DNA. Translation: DAA07256.1.
PIRiS46009.
RefSeqiNP_009698.3. NM_001178488.3.

Genome annotation databases

EnsemblFungiiYBR140C; YBR140C; YBR140C.
GeneIDi852437.
KEGGisce:YBR140C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36009 Genomic DNA. Translation: CAA85098.1 .
M24378 Genomic DNA. Translation: AAA34709.1 .
X75891 Genomic DNA. Translation: CAA53498.1 .
X78937 Genomic DNA. Translation: CAA55537.1 .
BK006936 Genomic DNA. Translation: DAA07256.1 .
PIRi S46009.
RefSeqi NP_009698.3. NM_001178488.3.

3D structure databases

ProteinModelPortali P18963.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32840. 34 interactions.
DIPi DIP-2318N.
IntActi P18963. 3 interactions.
MINTi MINT-688782.
STRINGi 4932.YBR140C.

Proteomic databases

MaxQBi P18963.
PaxDbi P18963.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR140C ; YBR140C ; YBR140C .
GeneIDi 852437.
KEGGi sce:YBR140C.

Organism-specific databases

CYGDi YBR140c.
SGDi S000000344. IRA1.

Phylogenomic databases

eggNOGi COG5261.
GeneTreei ENSGT00550000074797.
HOGENOMi HOG000113101.
OMAi FCIIADI.
OrthoDBi EOG7H1JTR.

Enzyme and pathway databases

BioCyci YEAST:G3O-29094-MONOMER.

Miscellaneous databases

NextBioi 971330.

Gene expression databases

Genevestigatori P18963.

Family and domain databases

Gene3Di 1.10.506.10. 1 hit.
InterProi IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view ]
Pfami PF00616. RasGAP. 1 hit.
[Graphical view ]
SMARTi SM00323. RasGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The sequence of 12.5 kb from the right arm of chromosome II predicts a new N-terminal sequence for the IRA1 protein and reveals two new genes, one of which is a DEAD-box helicase."
    Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A., Slonimski P.P., Sokolowska B., Herbert C.J.
    Yeast 10:1227-1234(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2767.
    Strain: ATCC 204508 / S288c.
  4. "IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae."
    Tanaka K., Matsumoto K., Toh-e A.
    Mol. Cell. Biol. 9:757-768(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-3092.
  5. "The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
    Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
    Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2768-3092.
    Strain: ATCC 204508 / S288c.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-915 AND SER-1342, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIRA1_YEAST
AccessioniPrimary (citable) accession number: P18963
Secondary accession number(s): D6VQD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi