P18963 (IRA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inhibitory regulator protein IRA1

Gene names

Name:	IRA1
Synonyms:	GLC1, PPD1
Ordered Locus Names:	YBR140C
ORF Names:	YBR1016

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	3092 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitory regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae. Stimulates the GTPase activity of Ras proteins.
Subcellular location	Cytoplasm Ref.6.
Sequence similarities	Contains 1 Ras-GAP domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of Ras protein signal transduction Inferred from mutant phenotype PubMed 15339905. Source: SGD negative regulation of cAMP biosynthetic process Inferred from mutant phenotype Ref.4. Source: SGD regulation of adenylate cyclase activity Inferred from mutant phenotype PubMed 1875942. Source: SGD signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD intrinsic to internal side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Molecular_function	Ras GTPase activator activity Inferred from mutant phenotype PubMed 2178777. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 3092	3092	Inhibitory regulator protein IRA1		PRO_0000056657
Regions
	Domain	1709 – 1898	190	Ras-GAP
	Compositional bias	326 – 331	6	Poly-Ser
	Compositional bias	379 – 383	5	Poly-Ser
	Compositional bias	475 – 484	10	Poly-Asn
	Compositional bias	1433 – 1437	5	Poly-Ile
	Compositional bias	2039 – 2042	4	Poly-Glu
Amino acid modifications
	Modified residue	497	1	Phosphoserine Ref.8
	Modified residue	892	1	Phosphoserine Ref.8
	Modified residue	915	1	Phosphoserine Ref.7
	Modified residue	1340	1	Phosphoserine Ref.8
	Modified residue	1342	1	Phosphoserine Ref.8
	Modified residue	1753	1	Phosphoserine; by PKA Potential
	Modified residue	3004	1	Phosphoserine; by PKA Potential
Experimental info
	Sequence conflict	361	1	V → G in AAA34709. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P18963 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 291339AE5BD26E08	FASTA	3,092	350,980
	10 20 30 40 50 60 MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN SCRSIILNIA 70 80 90 100 110 120 ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV LLRLLSDVFE YYWDQNNDFK 130 140 150 160 170 180 KIRNDNYKPG FSSHRPNFHT SRPKHTRINP ALATMLLCKI SKLKFNTRTL KVLQNMSHHL 190 200 210 220 230 240 SGSATISKSS ILPDSQEFLQ KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA 250 260 270 280 290 300 PLLISHTSTE LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA 310 320 330 340 350 360 FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL LFDDVYSTFS 370 380 390 400 410 420 VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK QSSVNASGNV SPSQFSTGND 430 440 450 460 470 480 ASPTSPMASL SSPLNTNILG YPLSPITSTL GQANTSTSTT AATTKTDADT PSTMNTNNNN 490 500 510 520 530 540 NNNNSANLNN IPQRIFSLDD ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG 550 560 570 580 590 600 VNNSQSQNDQ SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY 610 620 630 640 650 660 RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK FLTYLIRNLN 670 680 690 700 710 720 GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS KRFYNLLGQN LEVGTNWNSA 730 740 750 760 770 780 TANTFISHCV ERNPLTHRRL QLEFFASGLQ LDSDLFLRHL QLEKELNHID LPKISLYTEG 790 800 810 820 830 840 FRVFFHLVST KKLHEDIAEK TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL 850 860 870 880 890 900 DQFDAARTLS NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR 910 920 930 940 950 960 TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT TKRYASPNDS 970 980 990 1000 1010 1020 ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA TPTILKNIKS PKPNKTKKIA 1030 1040 1050 1060 1070 1080 DDKQLKQPSY SRVILSDNDE ARKIMMNIFS IFKRMTNWFI RPDANTEFPK TFTDIIKPLF 1090 1100 1110 1120 1130 1140 VSILDSNQRL QVTARAFIEI PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD 1150 1160 1170 1180 1190 1200 LKLENAKREM LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS 1210 1220 1230 1240 1250 1260 LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD NFTASGSVAL 1270 1280 1290 1300 1310 1320 QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT QEELVDFRSL AGILASMSGI 1330 1340 1350 1360 1370 1380 LSDMQELEKS KSAPDNEGDS LSFESRNPAY EVHKSLKLEL TKKMNFFISK QCQWLNNPNL 1390 1400 1410 1420 1430 1440 LTRENSRDIL SIELHPLSFN LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI 1450 1460 1470 1480 1490 1500 LKRDDDEKIM LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG 1510 1520 1530 1540 1550 1560 ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY IDTSIESAKA 1570 1580 1590 1600 1610 1620 LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL EKSADLNQFP VSLRHKISIL 1630 1640 1650 1660 1670 1680 NENVIIALTN LSNANVNVSL KFTLPMGYSP NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK 1690 1700 1710 1720 1730 1740 MLAIDDFLKY IIKNPILAFF GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK 1750 1760 1770 1780 1790 1800 RAARSDDILR RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN 1810 1820 1830 1840 1850 1860 SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY IAVGSFVFLR 1870 1880 1890 1900 1910 1920 FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR ENIFKKDILV SKEEFLKTCS 1930 1940 1950 1960 1970 1980 DKIFNFLSEL CKIPTNNFTV NVREDPTPIS FDYSFLHKFF YLNEFTIRKE IINESKLPGE 1990 2000 2010 2020 2030 2040 FSFLKNTVML NDKILGVLGQ PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE 2050 2060 2070 2080 2090 2100 EEDNAPFVHE AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT 2110 2120 2130 2140 2150 2160 TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV ENPYLVTTIP 2170 2180 2190 2200 2210 2220 RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD KEKKKFCPVS LKIGNKYFQV 2230 2240 2250 2260 2270 2280 LHEIPQLYKV TVSNRTFSIK FNNVYKISNL ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP 2290 2300 2310 2320 2330 2340 KYLEIVKMFY YAQLKMEEDF GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV 2350 2360 2370 2380 2390 2400 GLFNEDDLVK NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST 2410 2420 2430 2440 2450 2460 ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG PEAISRIIYS 2470 2480 2490 2500 2510 2520 LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH ALDRDSENRD WMKAVSILTS 2530 2540 2550 2560 2570 2580 FPTTEIACQV IEKLINMIKS FLPSLAVEAS AHSWSELTIL SKISVSIFFE SPLLSQMYLP 2590 2600 2610 2620 2630 2640 EILFAVSLLI DVGPSEIRVS LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF 2650 2660 2670 2680 2690 2700 ISGFSQEKGR VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA 2710 2720 2730 2740 2750 2760 IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM FIIIAHVFTY 2770 2780 2790 2800 2810 2820 SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM VNCLKRLYTV HLQLGFDGKS 2830 2840 2850 2860 2870 2880 LAKKLMESRN FAATLLAKLE SYNGCIWNED NFPHIILGFI ANGLSIPVVK GAALDCLQAL 2890 2900 2910 2920 2930 2940 FKNTYYERKS NPKSSDYLCY LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI 2950 2960 2970 2980 2990 3000 NWLSSDSDKS NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF 3010 3020 3030 3040 3050 3060 RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV VKPLDIFDQE 3070 3080 3090 HIEKLKGEGE HQVAIYERKR LATMILARMS CS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The sequence of 12.5 kb from the right arm of chromosome II predicts a new N-terminal sequence for the IRA1 protein and reveals two new genes, one of which is a DEAD-box helicase." Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A., Slonimski P.P., Sokolowska B., Herbert C.J. Yeast 10:1227-1234(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2767. Strain: ATCC 204508 / S288c.
[4]	"IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae." Tanaka K., Matsumoto K., Toh-e A. Mol. Cell. Biol. 9:757-768(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-3092.
[5]	"The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes." Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J. Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2768-3092. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-915, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-892; SER-1340 AND SER-1342, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36009 Genomic DNA. Translation: CAA85098.1. M24378 Genomic DNA. Translation: AAA34709.1. X75891 Genomic DNA. Translation: CAA53498.1. X78937 Genomic DNA. Translation: CAA55537.1. BK006936 Genomic DNA. Translation: DAA07256.1.
PIR	S46009.
RefSeq	NP_009698.3. NM_001178488.3.
3D structure databases
ProteinModelPortal	P18963.
SMR	P18963. Positions 1714-1961.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2318N.
IntAct	P18963. 4 interactions.
MINT	MINT-688782.
STRING	4932.YBR140C.
Proteomic databases
PaxDb	P18963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR140C; YBR140C; YBR140C.
GeneID	852437.
KEGG	sce:YBR140C.
Organism-specific databases
CYGD	YBR140c.
SGD	S000000344. IRA1.
Phylogenomic databases
eggNOG	COG5261.
GeneTree	ENSGT00550000074797.
HOGENOM	HOG000113101.
OMA	ARAMMIL.
OrthoDB	EOG4GF6P5.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29094-MONOMER.
Gene expression databases
Genevestigator	P18963.
GermOnline	YBR140C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.506.10. 1 hit.
InterPro	IPR001936. RasGAP. IPR023152. RasGAP_CS. IPR008936. Rho_GTPase_activation_prot. [Graphical view]
Pfam	PF00616. RasGAP. 1 hit. [Graphical view]
SMART	SM00323. RasGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS00509. RAS_GTPASE_ACTIV_1. 1 hit. PS50018. RAS_GTPASE_ACTIV_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971330.

Entry information

Entry name

IRA1_YEAST

Accession

Primary (citable) accession number: P18963
Secondary accession number(s): D6VQD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	October 1, 1994
Last modified:	May 29, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program