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P18963

- IRA1_YEAST

UniProt

P18963 - IRA1_YEAST

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Protein

Inhibitory regulator protein IRA1

Gene

IRA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Inhibitory regulator of the Ras-cyclic AMP pathway in Saccharomyces cerevisiae. Stimulates the GTPase activity of Ras proteins.

GO - Molecular functioni

  1. Ras GTPase activator activity Source: SGD

GO - Biological processi

  1. negative regulation of cAMP biosynthetic process Source: SGD
  2. negative regulation of Ras protein signal transduction Source: SGD
  3. positive regulation of Ras GTPase activity Source: SGD
  4. regulation of adenylate cyclase activity Source: SGD
  5. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciYEAST:G3O-29094-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitory regulator protein IRA1
Gene namesi
Name:IRA1
Synonyms:GLC1, PPD1
Ordered Locus Names:YBR140C
ORF Names:YBR1016
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR140c.
SGDiS000000344. IRA1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30923092Inhibitory regulator protein IRA1PRO_0000056657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei497 – 4971Phosphoserine2 Publications
Modified residuei915 – 9151Phosphoserine1 Publication
Modified residuei1342 – 13421Phosphoserine1 Publication
Modified residuei1753 – 17531Phosphoserine; by PKASequence Analysis
Modified residuei3004 – 30041Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP18963.
PaxDbiP18963.

Expressioni

Gene expression databases

GenevestigatoriP18963.

Interactioni

Protein-protein interaction databases

BioGridi32840. 34 interactions.
DIPiDIP-2318N.
IntActiP18963. 3 interactions.
MINTiMINT-688782.
STRINGi4932.YBR140C.

Structurei

3D structure databases

ProteinModelPortaliP18963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1709 – 1898190Ras-GAPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi326 – 3316Poly-Ser
Compositional biasi379 – 3835Poly-Ser
Compositional biasi475 – 48410Poly-Asn
Compositional biasi1433 – 14375Poly-Ile
Compositional biasi2039 – 20424Poly-Glu

Sequence similaritiesi

Contains 1 Ras-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5261.
GeneTreeiENSGT00550000074797.
HOGENOMiHOG000113101.
InParanoidiP18963.
OMAiFCIIADI.
OrthoDBiEOG7H1JTR.

Family and domain databases

Gene3Di1.10.506.10. 1 hit.
InterProiIPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]
PfamiPF00616. RasGAP. 1 hit.
[Graphical view]
SMARTiSM00323. RasGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18963-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQSDPQDKK NFPMEYSLTK HLFFDRLLLV LPIESNLKTY ADVEADSVFN
60 70 80 90 100
SCRSIILNIA ITKDLNPIIE NTLGLIDLIV QDEEITSDNI TDDIAHSILV
110 120 130 140 150
LLRLLSDVFE YYWDQNNDFK KIRNDNYKPG FSSHRPNFHT SRPKHTRINP
160 170 180 190 200
ALATMLLCKI SKLKFNTRTL KVLQNMSHHL SGSATISKSS ILPDSQEFLQ
210 220 230 240 250
KRNYPAYTEK IDLTIDYIQR FISASNHVEF TKCVKTKVVA PLLISHTSTE
260 270 280 290 300
LGVVNHLDLF GCEYLTDKNL LAYLDILQHL SSYMKRTIFH SLLLYYASKA
310 320 330 340 350
FLFWIMARPK EYVKIYNNLI SSDYNSPSSS SDNGGSNNSD KTSISQLVSL
360 370 380 390 400
LFDDVYSTFS VSSLLTNVNN DHHYHLHHSS SSSKTTNTNS PNSISKTSIK
410 420 430 440 450
QSSVNASGNV SPSQFSTGND ASPTSPMASL SSPLNTNILG YPLSPITSTL
460 470 480 490 500
GQANTSTSTT AATTKTDADT PSTMNTNNNN NNNNSANLNN IPQRIFSLDD
510 520 530 540 550
ISSFNSSRKS LNLDDSNSLF LWDTSQHSNA SMTNTNMHAG VNNSQSQNDQ
560 570 580 590 600
SSLNYMENIM ELYSNYTGSE LSSHTAILRF LVVLTLLDSE VYDEMNSNSY
610 620 630 640 650
RKISEPIMNI NPKDSNTSSW GSASKNPSIR HLTHGLKKLT LQQGRKRNVK
660 670 680 690 700
FLTYLIRNLN GGQFVSDVSL IDSIRSILFL MTMTSSISQI DSNIASVIFS
710 720 730 740 750
KRFYNLLGQN LEVGTNWNSA TANTFISHCV ERNPLTHRRL QLEFFASGLQ
760 770 780 790 800
LDSDLFLRHL QLEKELNHID LPKISLYTEG FRVFFHLVST KKLHEDIAEK
810 820 830 840 850
TSSVLKRLFC IIADILLKAT PYFDDNVTKI IASILDGHIL DQFDAARTLS
860 870 880 890 900
NDDHVSFDAA TSVYTEPTEI IHNSSDASLV SSLSQSPLSI NSGSNITNTR
910 920 930 940 950
TWDIQSILPT LSNRSSASDL SLSNILTNPL EAQQNNNANL LAHRLSGVPT
960 970 980 990 1000
TKRYASPNDS ERSRQSPYSS PPQLQQSDLP SPLSVLSSSA GFSSNHSITA
1010 1020 1030 1040 1050
TPTILKNIKS PKPNKTKKIA DDKQLKQPSY SRVILSDNDE ARKIMMNIFS
1060 1070 1080 1090 1100
IFKRMTNWFI RPDANTEFPK TFTDIIKPLF VSILDSNQRL QVTARAFIEI
1110 1120 1130 1140 1150
PLSYIATFED IDNDLDPRVL NDHYLLCTYA VTLFASSLFD LKLENAKREM
1160 1170 1180 1190 1200
LLDIIVKFQR VRSYLSNLAE KHNLVQAIIT TERLTLPLLV GAVGSGIFIS
1210 1220 1230 1240 1250
LYCSRGNTPR LIKISCCEFL RSLRFYQKYV GALDQYSIYN IDFIDAMAQD
1260 1270 1280 1290 1300
NFTASGSVAL QRRLRNNILT YIKGSDSILL DSMDVIYKKW FYFSCSKSVT
1310 1320 1330 1340 1350
QEELVDFRSL AGILASMSGI LSDMQELEKS KSAPDNEGDS LSFESRNPAY
1360 1370 1380 1390 1400
EVHKSLKLEL TKKMNFFISK QCQWLNNPNL LTRENSRDIL SIELHPLSFN
1410 1420 1430 1440 1450
LLFNNLGLKI DELMSIDLSK SHEDSSFVLL EQIIIIIRTI LKRDDDEKIM
1460 1470 1480 1490 1500
LLFSTDLLDA VDKLIEIVEK ISIKSSKYYK GIIQMSKMFR AFEHSEKNLG
1510 1520 1530 1540 1550
ISNHFHLKNK WLKLVIGWFK LSINKDYDFE NLSRPLREMD LQKRDEDFLY
1560 1570 1580 1590 1600
IDTSIESAKA LAYLTHNVPL EIPPSSSKED WNRSSTVSFG NHFTILLKGL
1610 1620 1630 1640 1650
EKSADLNQFP VSLRHKISIL NENVIIALTN LSNANVNVSL KFTLPMGYSP
1660 1670 1680 1690 1700
NKDIRIAFLR VFIDIVTNYP VNPEKHEMDK MLAIDDFLKY IIKNPILAFF
1710 1720 1730 1740 1750
GSLACSPADV DLYAGGFLNA FDTRNASHIL VTELLKQEIK RAARSDDILR
1760 1770 1780 1790 1800
RNSCATRALS LYTRSRGNKY LIKTLRPVLQ GIVDNKESFE IDKMKPGSEN
1810 1820 1830 1840 1850
SEKMLDLFEK YMTRLIDAIT SSIDDFPIEL VDICKTIYNA ASVNFPEYAY
1860 1870 1880 1890 1900
IAVGSFVFLR FIGPALVSPD SENIIIVTHA HDRKPFITLA KVIQSLANGR
1910 1920 1930 1940 1950
ENIFKKDILV SKEEFLKTCS DKIFNFLSEL CKIPTNNFTV NVREDPTPIS
1960 1970 1980 1990 2000
FDYSFLHKFF YLNEFTIRKE IINESKLPGE FSFLKNTVML NDKILGVLGQ
2010 2020 2030 2040 2050
PSMEIKNEIP PFVVENREKY PSLYEFMSRY AFKKVDMKEE EEDNAPFVHE
2060 2070 2080 2090 2100
AMTLDGIQII VVTFTNCEYN NFVMDSLVYK VLQIYARMWC SKHYVVIDCT
2110 2120 2130 2140 2150
TFYGGKANFQ KLTTLFFSLI PEQASSNCMG CYYFNVNKSF MDQWASSYTV
2160 2170 2180 2190 2200
ENPYLVTTIP RCFINSNTDQ SLIKSLGLSG RSLEVLKDVR VTLHDITLYD
2210 2220 2230 2240 2250
KEKKKFCPVS LKIGNKYFQV LHEIPQLYKV TVSNRTFSIK FNNVYKISNL
2260 2270 2280 2290 2300
ISVDVSNTTG VSSEFTLSLD NEEKLVFCSP KYLEIVKMFY YAQLKMEEDF
2310 2320 2330 2340 2350
GTDFSNDISF STSSSAVNAS YCNVKEVGEI ISHLSLVILV GLFNEDDLVK
2360 2370 2380 2390 2400
NISYNLLVAT QEAFNLDFGT RLHKSPETYV PDDTTTFLAL IFKAFSESST
2410 2420 2430 2440 2450
ELTPYIWKYM LDGLENDVIP QEHIPTVVCS LSYWVPNLYE HVYLANDEEG
2460 2470 2480 2490 2500
PEAISRIIYS LIRLTVKEPN FTTAYLQQIW FLLALDGRLT NVIVEEIVSH
2510 2520 2530 2540 2550
ALDRDSENRD WMKAVSILTS FPTTEIACQV IEKLINMIKS FLPSLAVEAS
2560 2570 2580 2590 2600
AHSWSELTIL SKISVSIFFE SPLLSQMYLP EILFAVSLLI DVGPSEIRVS
2610 2620 2630 2640 2650
LYELLMNVCH SLTNNESLPE RNRKNLDIVC ATFARQKLNF ISGFSQEKGR
2660 2670 2680 2690 2700
VLPNFAASSF SSKFGTLDLF TKNIMLLMEY GSISEGAQWE AKYKKYLMDA
2710 2720 2730 2740 2750
IFGHRSFFSA RAMMILGIMS KSHTSLFLCK ELLVETMKVF AEPVVDDEQM
2760 2770 2780 2790 2800
FIIIAHVFTY SKIVEGLDPS SELMKELFWL ATICVESPHP LLFEGGLLFM
2810 2820 2830 2840 2850
VNCLKRLYTV HLQLGFDGKS LAKKLMESRN FAATLLAKLE SYNGCIWNED
2860 2870 2880 2890 2900
NFPHIILGFI ANGLSIPVVK GAALDCLQAL FKNTYYERKS NPKSSDYLCY
2910 2920 2930 2940 2950
LFLLHLVLSP EQLSTLLLEV GFEDELVPLN NTLKVPLTLI NWLSSDSDKS
2960 2970 2980 2990 3000
NIVLYQGALL FSCVMSDEPC KFRFALLMRY LLKVNPICVF RFYTLTRKEF
3010 3020 3030 3040 3050
RRLSTLEQSS EAVAVSFELI GMLVTHSEFN YLEEFNDEMV ELLKKRGLSV
3060 3070 3080 3090
VKPLDIFDQE HIEKLKGEGE HQVAIYERKR LATMILARMS CS
Length:3,092
Mass (Da):350,980
Last modified:October 1, 1994 - v2
Checksum:i291339AE5BD26E08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 3611V → G in AAA34709. (PubMed:2540426)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36009 Genomic DNA. Translation: CAA85098.1.
M24378 Genomic DNA. Translation: AAA34709.1.
X75891 Genomic DNA. Translation: CAA53498.1.
X78937 Genomic DNA. Translation: CAA55537.1.
BK006936 Genomic DNA. Translation: DAA07256.1.
PIRiS46009.
RefSeqiNP_009698.3. NM_001178488.3.

Genome annotation databases

EnsemblFungiiYBR140C; YBR140C; YBR140C.
GeneIDi852437.
KEGGisce:YBR140C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36009 Genomic DNA. Translation: CAA85098.1 .
M24378 Genomic DNA. Translation: AAA34709.1 .
X75891 Genomic DNA. Translation: CAA53498.1 .
X78937 Genomic DNA. Translation: CAA55537.1 .
BK006936 Genomic DNA. Translation: DAA07256.1 .
PIRi S46009.
RefSeqi NP_009698.3. NM_001178488.3.

3D structure databases

ProteinModelPortali P18963.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32840. 34 interactions.
DIPi DIP-2318N.
IntActi P18963. 3 interactions.
MINTi MINT-688782.
STRINGi 4932.YBR140C.

Proteomic databases

MaxQBi P18963.
PaxDbi P18963.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR140C ; YBR140C ; YBR140C .
GeneIDi 852437.
KEGGi sce:YBR140C.

Organism-specific databases

CYGDi YBR140c.
SGDi S000000344. IRA1.

Phylogenomic databases

eggNOGi COG5261.
GeneTreei ENSGT00550000074797.
HOGENOMi HOG000113101.
InParanoidi P18963.
OMAi FCIIADI.
OrthoDBi EOG7H1JTR.

Enzyme and pathway databases

BioCyci YEAST:G3O-29094-MONOMER.

Miscellaneous databases

NextBioi 971330.

Gene expression databases

Genevestigatori P18963.

Family and domain databases

Gene3Di 1.10.506.10. 1 hit.
InterProi IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view ]
Pfami PF00616. RasGAP. 1 hit.
[Graphical view ]
SMARTi SM00323. RasGAP. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
PROSITEi PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The sequence of 12.5 kb from the right arm of chromosome II predicts a new N-terminal sequence for the IRA1 protein and reveals two new genes, one of which is a DEAD-box helicase."
    Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A., Slonimski P.P., Sokolowska B., Herbert C.J.
    Yeast 10:1227-1234(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-2767.
    Strain: ATCC 204508 / S288c.
  4. "IRA1, an inhibitory regulator of the RAS-cyclic AMP pathway in Saccharomyces cerevisiae."
    Tanaka K., Matsumoto K., Toh-e A.
    Mol. Cell. Biol. 9:757-768(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 155-3092.
  5. "The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes."
    Becam A.-M., Cullin C., Grzybowska E., Lacroute F., Nasr F., Ozier-Kalogeropoulos O., Palucha A., Slonimski P.P., Zagulski M., Herbert C.J.
    Yeast 10:S1-S11(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2768-3092.
    Strain: ATCC 204508 / S288c.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497; SER-915 AND SER-1342, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIRA1_YEAST
AccessioniPrimary (citable) accession number: P18963
Secondary accession number(s): D6VQD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3