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Protein

Dipeptidyl aminopeptidase B

Gene

DAP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei679Charge relay systemPROSITE-ProRule annotation1
Active sitei756Charge relay systemPROSITE-ProRule annotation1
Active sitei789Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • dipeptidyl-peptidase activity Source: SGD
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • protein processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciYEAST:G3O-31088-MONOMER.

Protein family/group databases

ESTHERiyeast-dap2. DPP4N_Peptidase_S9.
MEROPSiS09.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Dipeptidyl aminopeptidase B (EC:3.4.14.-)
Short name:
DPAP B
Alternative name(s):
YSCV
Gene namesi
Name:DAP2
Ordered Locus Names:YHR028C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR028C.
SGDiS000001070. DAP2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicSequence analysisAdd BLAST29
Transmembranei30 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini46 – 818LumenalSequence analysisAdd BLAST773

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224211 – 818Dipeptidyl aminopeptidase BAdd BLAST818

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Glycosylationi79N-linked (GlcNAc...)Sequence analysis1
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi139N-linked (GlcNAc...)Sequence analysis1
Glycosylationi372N-linked (GlcNAc...)Sequence analysis1
Glycosylationi392N-linked (GlcNAc...)Sequence analysis1
Glycosylationi421N-linked (GlcNAc...)Sequence analysis1
Glycosylationi738N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP18962.
PRIDEiP18962.

Interactioni

Protein-protein interaction databases

BioGridi36459. 50 interactors.
DIPiDIP-5759N.

Structurei

Secondary structure

1818
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 37Combined sources4
Turni38 – 41Combined sources4
Helixi42 – 47Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KL4X-ray3.50B26-51[»]
3ZN8electron microscopy12.00S31-44[»]
ProteinModelPortaliP18962.
SMRiP18962.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18962.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000119233.
HOGENOMiHOG000189891.
InParanoidiP18962.
KOiK01282.
OMAiCKSPLWH.
OrthoDBiEOG092C0PZJ.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B_N.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGGEEEVER IPDELFDTKK KHLLDKLIRV GIILVLLIWG TVLLLKSIPH
60 70 80 90 100
HSNTPDYQEP NSNYTNDGKL KVSFSVVRNN TFQPKYHELQ WISDNKIESN
110 120 130 140 150
DLGLYVTFMN DSYVVKSVYD DSYNSVLLEG KTFIHNGQNL TVESITASPD
160 170 180 190 200
LKRLLIRTNS VQNWRHSTFG SYFVYDKSSS SFEEIGNEVA LAIWSPNSND
210 220 230 240 250
IAYVQDNNIY IYSAISKKTI RAVTNDGSSF LFNGKPDWVY EEEVFEDDKA
260 270 280 290 300
AWWSPTGDYL AFLKIDESEV GEFIIPYYVQ DEKDIYPEMR SIKYPKSGTP
310 320 330 340 350
NPHAELWVYS MKDGTSFHPR ISGNKKDGSL LITEVTWVGN GNVLVKTTDR
360 370 380 390 400
SSDILTVFLI DTIAKTSNVV RNESSNGGWW EITHNTLFIP ANETFDRPHN
410 420 430 440 450
GYVDILPIGG YNHLAYFENS NSSHYKTLTE GKWEVVNGPL AFDSMENRLY
460 470 480 490 500
FISTRKSSTE RHVYYIDLRS PNEIIEVTDT SEDGVYDVSF SSGRRFGLLT
510 520 530 540 550
YKGPKVPYQK IVDFHSRKAE KCDKGNVLGK SLYHLEKNEV LTKILEDYAV
560 570 580 590 600
PRKSFRELNL GKDEFGKDIL VNSYEILPND FDETLSDHYP VFFFAYGGPN
610 620 630 640 650
SQQVVKTFSV GFNEVVASQL NAIVVVVDGR GTGFKGQDFR SLVRDRLGDY
660 670 680 690 700
EARDQISAAS LYGSLTFVDP QKISLFGWSY GGYLTLKTLE KDGGRHFKYG
710 720 730 740 750
MSVAPVTDWR FYDSVYTERY MHTPQENFDG YVESSVHNVT ALAQANRFLL
760 770 780 790 800
MHGTGDDNVH FQNSLKFLDL LDLNGVENYD VHVFPDSDHS IRYHNANVIV
810
FDKLLDWAKR AFDGQFVK
Length:818
Mass (Da):93,404
Last modified:February 1, 1995 - v2
Checksum:i318F450445375BD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83Q → H in CAA33512 (PubMed:2647766).Curated1
Sequence conflicti125S → N in CAA33512 (PubMed:2647766).Curated1
Sequence conflicti182 – 188FEEIGNE → LRRLET in CAA33512 (PubMed:2647766).Curated7
Sequence conflicti200D → N in CAA33512 (PubMed:2647766).Curated1
Sequence conflicti318H → R in AAU09739 (PubMed:17322287).Curated1
Sequence conflicti366 – 375TSNVVRNESS → DFKRGKERKF in CAA33512 (PubMed:2647766).Curated10
Sequence conflicti808 – 818AKRAFDGQFVK → QSVLSMGNLTNELTIYSSSH RDIHKTFSYLHTMYI in CAA33512 (PubMed:2647766).CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15484 Genomic DNA. Translation: CAA33512.1.
U10399 Genomic DNA. Translation: AAB68879.1.
AY723822 Genomic DNA. Translation: AAU09739.1.
BK006934 Genomic DNA. Translation: DAA06719.1.
PIRiS46780. A30107.
RefSeqiNP_011893.1. NM_001179158.1.

Genome annotation databases

EnsemblFungiiYHR028C; YHR028C; YHR028C.
GeneIDi856423.
KEGGisce:YHR028C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15484 Genomic DNA. Translation: CAA33512.1.
U10399 Genomic DNA. Translation: AAB68879.1.
AY723822 Genomic DNA. Translation: AAU09739.1.
BK006934 Genomic DNA. Translation: DAA06719.1.
PIRiS46780. A30107.
RefSeqiNP_011893.1. NM_001179158.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KL4X-ray3.50B26-51[»]
3ZN8electron microscopy12.00S31-44[»]
ProteinModelPortaliP18962.
SMRiP18962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36459. 50 interactors.
DIPiDIP-5759N.

Protein family/group databases

ESTHERiyeast-dap2. DPP4N_Peptidase_S9.
MEROPSiS09.006.

Proteomic databases

MaxQBiP18962.
PRIDEiP18962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR028C; YHR028C; YHR028C.
GeneIDi856423.
KEGGisce:YHR028C.

Organism-specific databases

EuPathDBiFungiDB:YHR028C.
SGDiS000001070. DAP2.

Phylogenomic databases

GeneTreeiENSGT00760000119233.
HOGENOMiHOG000189891.
InParanoidiP18962.
KOiK01282.
OMAiCKSPLWH.
OrthoDBiEOG092C0PZJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31088-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP18962.
PROiP18962.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B_N.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAP2_YEAST
AccessioniPrimary (citable) accession number: P18962
Secondary accession number(s): D3DKX5, E9P957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.