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Protein

Gamma-glutamyltranspeptidase

Gene

ggt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid.1 Publication
Glutathione + H2O = L-cysteinylglycine + L-glutamate.By similarity

Pathwayi: glutathione metabolism

This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114Glutamate1 Publication1
Active sitei391Nucleophile2 Publications1
Binding sitei409Glutamate1 Publication1
Binding sitei411Glutamate1 Publication1
Binding sitei430Glutamate1 Publication1
Binding sitei433Glutamate1 Publication1

GO - Molecular functioni

  • gamma-glutamyltransferase activity Source: EcoliWiki
  • glutathione hydrolase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Hydrolase, Protease, Transferase

Keywords - Biological processi

Glutathione biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10374-MONOMER.
ECOL316407:JW3412-MONOMER.
MetaCyc:EG10374-MONOMER.
BRENDAi2.3.2.2. 2026.
3.4.19.13. 2026.
SABIO-RKP18956.
UniPathwayiUPA00204.

Protein family/group databases

MEROPSiT03.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyltranspeptidase1 Publication (EC:2.3.2.22 Publications)
Alternative name(s):
Glutathione hydrolase (EC:3.4.19.13By similarity)
Cleaved into the following 2 chains:
Gene namesi
Name:ggt
Ordered Locus Names:b3447, JW3412
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10374. ggt.

Subcellular locationi

GO - Cellular componenti

  • periplasmic space Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi391T → A: Abolishes autocatalytic cleavage. 1 Publication1
Mutagenesisi513R → A: Not processed into its subunits. 1 Publication1
Mutagenesisi571R → G: Not processed into its subunits. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000001105226 – 390Gamma-glutamyltranspeptidase large chainAdd BLAST365
ChainiPRO_0000011053391 – 580Gamma-glutamyltranspeptidase small chainAdd BLAST190

Post-translational modificationi

Cleaved by autocatalysis into a large and a small subunit.

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP18956.
PRIDEiP18956.

Interactioni

Subunit structurei

This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.4 Publications

Protein-protein interaction databases

BioGridi4261666. 368 interactors.
DIPiDIP-9758N.
IntActiP18956. 1 interactor.
MINTiMINT-1260227.
STRINGi511145.b3447.

Structurei

Secondary structure

1580
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 41Combined sources3
Beta strandi44 – 48Combined sources5
Beta strandi50 – 54Combined sources5
Helixi56 – 67Combined sources12
Helixi72 – 86Combined sources15
Turni88 – 90Combined sources3
Beta strandi93 – 102Combined sources10
Beta strandi108 – 113Combined sources6
Turni123 – 126Combined sources4
Beta strandi129 – 131Combined sources3
Helixi135 – 139Combined sources5
Helixi142 – 144Combined sources3
Helixi150 – 161Combined sources12
Helixi166 – 179Combined sources14
Helixi185 – 193Combined sources9
Helixi195 – 197Combined sources3
Helixi199 – 201Combined sources3
Helixi203 – 209Combined sources7
Helixi225 – 237Combined sources13
Helixi240 – 243Combined sources4
Helixi246 – 257Combined sources12
Helixi264 – 269Combined sources6
Beta strandi273 – 275Combined sources3
Beta strandi278 – 282Combined sources5
Beta strandi285 – 289Combined sources5
Helixi296 – 307Combined sources12
Helixi312 – 315Combined sources4
Helixi320 – 340Combined sources21
Turni344 – 346Combined sources3
Helixi351 – 354Combined sources4
Helixi357 – 364Combined sources8
Helixi375 – 377Combined sources3
Helixi384 – 386Combined sources3
Beta strandi392 – 397Combined sources6
Beta strandi403 – 409Combined sources7
Turni413 – 416Combined sources4
Helixi421 – 423Combined sources3
Helixi430 – 433Combined sources4
Beta strandi434 – 437Combined sources4
Beta strandi467 – 471Combined sources5
Beta strandi474 – 479Combined sources6
Helixi484 – 486Combined sources3
Helixi487 – 499Combined sources13
Helixi505 – 510Combined sources6
Beta strandi518 – 520Combined sources3
Beta strandi523 – 525Combined sources3
Helixi531 – 539Combined sources9
Beta strandi544 – 546Combined sources3
Beta strandi554 – 558Combined sources5
Beta strandi564 – 568Combined sources5
Beta strandi576 – 579Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DBUX-ray1.95A/C25-390[»]
B/D391-580[»]
2DBWX-ray1.80A/C25-390[»]
B/D391-580[»]
2DBXX-ray1.70A/C25-390[»]
B/D391-580[»]
2DG5X-ray1.60A/C25-390[»]
B/D391-580[»]
2E0WX-ray2.55A/B25-580[»]
2E0XX-ray1.95A/C25-390[»]
B/D391-580[»]
2E0YX-ray2.02A/C25-390[»]
B/D391-580[»]
2Z8IX-ray1.65A/C25-390[»]
B/D391-580[»]
2Z8JX-ray2.05A/C25-390[»]
B/D391-580[»]
2Z8KX-ray1.65A/C25-390[»]
B/D391-580[»]
5B5TX-ray1.70A/C25-390[»]
B/D391-580[»]
ProteinModelPortaliP18956.
SMRiP18956.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18956.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni462 – 463Glutamate binding2
Regioni483 – 484Glutamate binding2

Sequence similaritiesi

Belongs to the gamma-glutamyltransferase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CFB. Bacteria.
COG0405. LUCA.
HOGENOMiHOG000175617.
InParanoidiP18956.
KOiK00681.
OMAiSTSHFVI.
PhylomeDBiP18956.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR00066. g_glut_trans. 1 hit.
PROSITEiPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKPTFLRRV AIAALLSGSC FSAAAAPPAP PVSYGVEEDV FHPVRAKQGM
60 70 80 90 100
VASVDATATQ VGVDILKEGG NAVDAAVAVG YALAVTHPQA GNLGGGGFML
110 120 130 140 150
IRSKNGNTTA IDFREMAPAK ATRDMFLDDQ GNPDSKKSLT SHLASGTPGT
160 170 180 190 200
VAGFSLALDK YGTMPLNKVV QPAFKLARDG FIVNDALADD LKTYGSEVLP
210 220 230 240 250
NHENSKAIFW KEGEPLKKGD TLVQANLAKS LEMIAENGPD EFYKGTIAEQ
260 270 280 290 300
IAQEMQKNGG LITKEDLAAY KAVERTPISG DYRGYQVYSM PPPSSGGIHI
310 320 330 340 350
VQILNILENF DMKKYGFGSA DAMQIMAEAE KYAYADRSEY LGDPDFVKVP
360 370 380 390 400
WQALTNKAYA KSIADQIDIN KAKPSSEIRP GKLAPYESNQ TTHYSVVDKD
410 420 430 440 450
GNAVAVTYTL NTTFGTGIVA GESGILLNNQ MDDFSAKPGV PNVYGLVGGD
460 470 480 490 500
ANAVGPNKRP LSSMSPTIVV KDGKTWLVTG SPGGSRIITT VLQMVVNSID
510 520 530 540 550
YGLNVAEATN APRFHHQWLP DELRVEKGFS PDTLKLLEAK GQKVALKEAM
560 570 580
GSTQSIMVGP DGELYGASDP RSVDDLTAGY
Length:580
Mass (Da):61,768
Last modified:November 1, 1990 - v1
Checksum:i772F652EBA2A5F00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28722 Genomic DNA. Translation: AAA23869.1.
U18997 Genomic DNA. Translation: AAA58245.1.
U00096 Genomic DNA. Translation: AAC76472.1.
AP009048 Genomic DNA. Translation: BAE77846.1.
U00039 Genomic DNA. Translation: AAB18422.1.
PIRiJV0028. EKECEX.
RefSeqiNP_417904.1. NC_000913.3.
WP_000595082.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76472; AAC76472; b3447.
BAE77846; BAE77846; BAE77846.
GeneIDi947947.
KEGGiecj:JW3412.
eco:b3447.
PATRICi32122334. VBIEscCol129921_3544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28722 Genomic DNA. Translation: AAA23869.1.
U18997 Genomic DNA. Translation: AAA58245.1.
U00096 Genomic DNA. Translation: AAC76472.1.
AP009048 Genomic DNA. Translation: BAE77846.1.
U00039 Genomic DNA. Translation: AAB18422.1.
PIRiJV0028. EKECEX.
RefSeqiNP_417904.1. NC_000913.3.
WP_000595082.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DBUX-ray1.95A/C25-390[»]
B/D391-580[»]
2DBWX-ray1.80A/C25-390[»]
B/D391-580[»]
2DBXX-ray1.70A/C25-390[»]
B/D391-580[»]
2DG5X-ray1.60A/C25-390[»]
B/D391-580[»]
2E0WX-ray2.55A/B25-580[»]
2E0XX-ray1.95A/C25-390[»]
B/D391-580[»]
2E0YX-ray2.02A/C25-390[»]
B/D391-580[»]
2Z8IX-ray1.65A/C25-390[»]
B/D391-580[»]
2Z8JX-ray2.05A/C25-390[»]
B/D391-580[»]
2Z8KX-ray1.65A/C25-390[»]
B/D391-580[»]
5B5TX-ray1.70A/C25-390[»]
B/D391-580[»]
ProteinModelPortaliP18956.
SMRiP18956.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261666. 368 interactors.
DIPiDIP-9758N.
IntActiP18956. 1 interactor.
MINTiMINT-1260227.
STRINGi511145.b3447.

Protein family/group databases

MEROPSiT03.001.

Proteomic databases

PaxDbiP18956.
PRIDEiP18956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76472; AAC76472; b3447.
BAE77846; BAE77846; BAE77846.
GeneIDi947947.
KEGGiecj:JW3412.
eco:b3447.
PATRICi32122334. VBIEscCol129921_3544.

Organism-specific databases

EchoBASEiEB0369.
EcoGeneiEG10374. ggt.

Phylogenomic databases

eggNOGiENOG4105CFB. Bacteria.
COG0405. LUCA.
HOGENOMiHOG000175617.
InParanoidiP18956.
KOiK00681.
OMAiSTSHFVI.
PhylomeDBiP18956.

Enzyme and pathway databases

UniPathwayiUPA00204.
BioCyciEcoCyc:EG10374-MONOMER.
ECOL316407:JW3412-MONOMER.
MetaCyc:EG10374-MONOMER.
BRENDAi2.3.2.2. 2026.
3.4.19.13. 2026.
SABIO-RKP18956.

Miscellaneous databases

EvolutionaryTraceiP18956.
PROiP18956.

Family and domain databases

InterProiIPR000101. GGT_peptidase.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PANTHERiPTHR11686. PTHR11686. 1 hit.
PfamiPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSiPR01210. GGTRANSPTASE.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR00066. g_glut_trans. 1 hit.
PROSITEiPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGGT_ECOLI
AccessioniPrimary (citable) accession number: P18956
Secondary accession number(s): Q2M7B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.