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P18956 (GGT_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-glutamyltranspeptidase

EC=2.3.2.2
Gene names
Name:ggt
Ordered Locus Names:b3447, JW3412
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid.

Glutathione + H2O = L-cysteinylglycine + L-glutamate.

Pathway

Sulfur metabolism; glutathione metabolism.

Subunit structure

This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the gamma-glutamyltransferase family.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionAcyltransferase
Transferase
   PTMZymogen
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological processglutathione biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiongamma-glutamyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.1
Chain26 – 390365Gamma-glutamyltranspeptidase large chain
PRO_0000011052
Chain391 – 580190Gamma-glutamyltranspeptidase small chain
PRO_0000011053

Experimental info

Mutagenesis5131R → A: Not processed into its subunits.
Mutagenesis5711R → G: Not processed into its subunits.

Secondary structure

.................................................................................................... 580
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18956 [UniParc].

Last modified November 1, 1990. Version 1.
Checksum: 772F652EBA2A5F00

FASTA58061,768
        10         20         30         40         50         60 
MIKPTFLRRV AIAALLSGSC FSAAAAPPAP PVSYGVEEDV FHPVRAKQGM VASVDATATQ 

        70         80         90        100        110        120 
VGVDILKEGG NAVDAAVAVG YALAVTHPQA GNLGGGGFML IRSKNGNTTA IDFREMAPAK 

       130        140        150        160        170        180 
ATRDMFLDDQ GNPDSKKSLT SHLASGTPGT VAGFSLALDK YGTMPLNKVV QPAFKLARDG 

       190        200        210        220        230        240 
FIVNDALADD LKTYGSEVLP NHENSKAIFW KEGEPLKKGD TLVQANLAKS LEMIAENGPD 

       250        260        270        280        290        300 
EFYKGTIAEQ IAQEMQKNGG LITKEDLAAY KAVERTPISG DYRGYQVYSM PPPSSGGIHI 

       310        320        330        340        350        360 
VQILNILENF DMKKYGFGSA DAMQIMAEAE KYAYADRSEY LGDPDFVKVP WQALTNKAYA 

       370        380        390        400        410        420 
KSIADQIDIN KAKPSSEIRP GKLAPYESNQ TTHYSVVDKD GNAVAVTYTL NTTFGTGIVA 

       430        440        450        460        470        480 
GESGILLNNQ MDDFSAKPGV PNVYGLVGGD ANAVGPNKRP LSSMSPTIVV KDGKTWLVTG 

       490        500        510        520        530        540 
SPGGSRIITT VLQMVVNSID YGLNVAEATN APRFHHQWLP DELRVEKGFS PDTLKLLEAK 

       550        560        570        580 
GQKVALKEAM GSTQSIMVGP DGELYGASDP RSVDDLTAGY 

« Hide

References

« Hide 'large scale' references
[1]"DNA sequence of the Escherichia coli K-12 gamma-glutamyltranspeptidase gene, ggt."
Suzuki H., Kumagai H., Echigo T., Tochikura T.
J. Bacteriol. 171:5169-5172(1989) [PubMed: 2570061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-41 AND 391-403.
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-362.
Strain: K12 / MG1655 / ATCC 47076.
[5]"Escherichia coli gamma-glutamyltranspeptidase mutants deficient in processing to subunits."
Hashimoto W., Suzuki H., Nohara S., Kumagai H.
Biochem. Biophys. Res. Commun. 189:173-178(1992) [PubMed: 1360205] [Abstract]
Cited for: MUTAGENESIS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28722 Genomic DNA. Translation: AAA23869.1.
U18997 Genomic DNA. Translation: AAA58245.1.
U00096 Genomic DNA. Translation: AAC76472.1.
AP009048 Genomic DNA. Translation: BAE77846.1.
U00039 Genomic DNA. Translation: AAB18422.1.
PIREKECEX. JV0028.
RefSeqNP_417904.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DBUX-ray1.95A/C25-390[»]
B/D391-580[»]
2DBWX-ray1.80A/C25-390[»]
B/D391-580[»]
2DBXX-ray1.70A/C25-390[»]
B/D391-580[»]
2DG5X-ray1.60A/C25-390[»]
B/D391-580[»]
2E0WX-ray2.55A/B25-580[»]
2E0XX-ray1.95A/C25-390[»]
B/D391-580[»]
2E0YX-ray2.02A/C25-390[»]
B/D391-580[»]
2Z8IX-ray1.65A/C25-390[»]
B/D391-580[»]
2Z8JX-ray2.05A/C25-390[»]
B/D391-580[»]
2Z8KX-ray1.65A/C25-390[»]
B/D391-580[»]
ProteinModelPortalP18956.
SMRP18956. Positions 29-580.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9758N.
IntActP18956. 1 interaction.
MINTMINT-1260227.

Protein family/group databases

MEROPST03.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000001721; EBESCP00000001721; EBESCG00000001418.
EBESCT00000016468; EBESCP00000015759; EBESCG00000015528.
GeneID947947.
GenomeReviewsGene locus JW3412 in contig AP009048_GR.
Gene locus b3447 in contig U00096_GR.
KEGGecj:JW3412.
eco:b3447.
PATRIC32122334. VBIEscCol129921_3544.

Organism-specific databases

EchoBASEEB0369.
EcoGeneEG10374. ggt.

Phylogenomic databases

eggNOGCOG0405.
GeneTreeEBGT00050000010331.
HOGENOMHBG738311.
OMAKYGTMTM.
PhylomeDBP18956.
ProtClustDBPRK09615.

Enzyme and pathway databases

BioCycEcoCyc:EG10374-MONOMER.
MetaCyc:EG10374-MONOMER.

Gene expression databases

GenevestigatorP18956.

Family and domain databases

InterProIPR000101. GGT_peptidase.
[Graphical view]
KOK00681.
PANTHERPTHR11686. GGT_peptidase. 1 hit.
PfamPF01019. G_glu_transpept. 1 hit.
[Graphical view]
PRINTSPR01210. GGTRANSPTASE.
TIGRFAMsTIGR00066. G_glut_trans. 1 hit.
PROSITEPS00462. G_GLU_TRANSPEPTIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

DrugBankDB01212. Ceftriaxone.

Entry information

Entry nameGGT_ECOLI
AccessionPrimary (citable) accession number: P18956
Secondary accession number(s): Q2M7B0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: January 25, 2012
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families