Reviewed, UniProtKB/Swiss-Prot P18952 (PA1_SERLI)

Names and origin

Protein names

Recommended name:
    Extracellular phospholipase A1
    EC=3.1.1.32

Gene names

Name:

phlA

Organism

Serratia liquefaciens

Taxonomic identifier

614 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.
Developmental stage	Growth phase regulated (late expression).

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phospholipase A1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 319	295	Extracellular phospholipase A1		PRO_0000021990
Experimental info
	Mutagenesis	228	1	G → D: Loss of ability to bind phlB. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P18952-1 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: FFEC47676E9A227B	FASTA	319	32,848
	10 20 30 40 50 60 MSMPLSFTSA VSPVAAIPTP RAAAETRTAA SLRHAGKSGP VASPSQNTLN AQNLLNTLVG 70 80 90 100 110 120 DISAAAPTAA AAPGVTRGQQ SQEGDYALAL LAKDVYSLNG QGAAGFNRLS DSALLGFGID 130 140 150 160 170 180 PASLHDAGSG FQAGIYSNDK QYVLAFAGTN DWRDWLSNVR QATGYDDVQY NQAVAAAKSA 190 200 210 220 230 240 KAAFGDALVI AGHSLGGGLA ATAALATGTV AVTFNAAGVS DYTLNRLGID PAAAKKDAEA 250 260 270 280 290 300 GGIRRYSEQY DMLTSTQEST SLIPDAIGHN ITLANNDTLT GIDDWRPSKH LDRSLTAHGI 310 DKVISSMAEQ KPWEAKANA

References

[1]	"Cloning and expression in Escherichia coli of the gene for extracellular phospholipase A1 from Serratia liquefaciens." Givskov M., Olsen L., Molin S. J. Bacteriol. 170:5855-5862(1988) [PubMed: 3056919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Secretion of Serratia liquefaciens phospholipase from Escherichia coli." Givskov M., Molin S. Mol. Microbiol. 8:229-242(1993) [PubMed: 8316077] [Abstract] Cited for: SEQUENCE REVISION TO 200-245, MUTAGENESIS OF GLY-228.
[3]	"Expression of extracellular phospholipase from Serratia liquefaciens is growth-phase-dependent, catabolite-repressed and regulated by anaerobiosis." Givskov M., Molin S. Mol. Microbiol. 6:1363-1374(1992) [PubMed: 1640837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-119.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M23640 mRNA. Translation: AAA26552.1. Sequence problems. X66505 Genomic DNA. Translation: CAA47137.1.
PIR	S32923.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.32. 141752.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

PA1_SERLI

Accession

Primary (citable) accession number: P18952
Secondary accession number(s): Q6LBK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	February 1, 1995
Last modified:	May 5, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)