Reviewed, UniProtKB/Swiss-Prot P18949 (METC_SALTY)

Names and origin

Protein names

Recommended name:
    Cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase

Gene names

Name:	metC
Ordered Locus Names:	STM3161

Organism

Salmonella typhimurium [Complete proteome] [HAMAP]

Taxonomic identifier

90371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Cystathionine beta-lyase		PRO_0000114769
Amino acid modifications
	Modified residue	210	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	195	1	K → E Ref.1
	Sequence conflict	200	1	D → H Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P18949-1 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 38B0A41A1A063447	FASTA	395	42,872
	10 20 30 40 50 60 MTDKQLDTKL VNAGRSKKYT LGSVNSVIQR ASSLVFDTVE AKKHATRNLA NGELFYGRRG 70 80 90 100 110 120 TLTHFSLQEA MCELEGGAGC ALFPCGAAAV ANTILAFVEQ GDHILMTNTA YEPSQDFCSK 130 140 150 160 170 180 ILGKLGVTTS WFDPLIGADI TQHIQPNTKV VFLESPGSIT MEVHDIPSIV SAVRRVAPEA 190 200 210 220 230 240 VIMIDNTWAA GVLFKALEFD IDISIQAGTK YLIGHSDAMV GTAVANARCW EQLRENAYLM 250 260 270 280 290 300 GQMLDADTAY MTSRGLRTLG VRLRQHQESS LKIAAWLANH PQVARVNHPA LPGSKGHAFW 310 320 330 340 350 360 KRDFTGSSGL FSFVLNKKLT EAELSAYLDN FSLFSMAYSW GGYESLIIAN QPEQIAAIRP 370 380 390 AGGVDFTGTL VRVHIGLESV DDLIADLAAG FARIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence of the metC gene and its flanking regions from Salmonella typhimurium LT2 and homology with the corresponding sequence of Escherichia coli." Park Y.M., Stauffer G.V. Mol. Gen. Genet. 216:164-169(1989) [PubMed: 2659970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LT2.
[2]	"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. , Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K. Nature 413:852-856(2001) [PubMed: 11677609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LT2 / SGSC1412 / ATCC 700720.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006468 Genomic DNA. Translation: AAL22035.1.
PIR	JV0020.
RefSeq	NP_462076.1.
3D structure databases
HSSP	HSSP built from PDB template 1CL2 based on UniProtKB P06721.
SMR	P18949. Positions 4-395.
ModBase	Search...
Proteomic databases
PRIDE	P18949.
Genome annotation databases
GeneID	1254684.
GenomeReviews	Gene locus STM3161 in contig AE006468_GR.
KEGG	stm:STM3161.
NMPDR	fig|99287.1.peg.3052.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P18949.
OMA	IETTYYD.
Enzyme and pathway databases
BioCyc	STYP99287:STM3161-MON.
BRENDA	4.4.1.8. 2.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006233. Cys_b_lyase_bac. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808:SF8. Cys_b_lyase_bac. 1 hit. PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
TIGRFAMs	TIGR01324. cysta_beta_ly_B. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METC_SALTY

Accession

Primary (citable) accession number: P18949

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	January 23, 2002
Last modified:	November 3, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)