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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk), Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI_1)
  4. Enolase (eno), Enolase (eno), Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (AA904_05055), Pyruvate kinase (pyk), Pyruvate kinase (GT94_09925), Pyruvate kinase (B4114_2369)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Binding sitei118SubstrateBy similarity1
Binding sitei151SubstrateBy similarity1
Binding sitei201ATP1
Binding sitei316ATP1
Binding sitei323ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi350 – 353ATP4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.3. 623.
SABIO-RKP18912.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001459041 – 394Phosphoglycerate kinaseAdd BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphoserineBy similarity1
Modified residuei299PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Beta strandi15 – 19Combined sources5
Beta strandi30 – 32Combined sources3
Helixi35 – 49Combined sources15
Beta strandi53 – 57Combined sources5
Helixi69 – 71Combined sources3
Helixi74 – 84Combined sources11
Beta strandi94 – 96Combined sources3
Helixi97 – 104Combined sources8
Beta strandi111 – 113Combined sources3
Helixi117 – 119Combined sources3
Helixi122 – 125Combined sources4
Helixi128 – 135Combined sources8
Beta strandi139 – 143Combined sources5
Helixi146 – 148Combined sources3
Turni154 – 157Combined sources4
Helixi158 – 161Combined sources4
Beta strandi165 – 167Combined sources3
Helixi169 – 183Combined sources15
Beta strandi187 – 193Combined sources7
Helixi198 – 208Combined sources11
Turni209 – 211Combined sources3
Beta strandi213 – 217Combined sources5
Helixi221 – 228Combined sources8
Helixi240 – 242Combined sources3
Helixi243 – 256Combined sources14
Beta strandi259 – 261Combined sources3
Beta strandi264 – 273Combined sources10
Beta strandi278 – 282Combined sources5
Helixi283 – 285Combined sources3
Beta strandi291 – 295Combined sources5
Helixi297 – 308Combined sources12
Beta strandi311 – 317Combined sources7
Helixi325 – 327Combined sources3
Helixi329 – 340Combined sources12
Beta strandi345 – 348Combined sources4
Helixi351 – 359Combined sources9
Helixi363 – 365Combined sources3
Beta strandi366 – 369Combined sources4
Helixi374 – 380Combined sources7
Helixi386 – 389Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PHPX-ray1.65A1-394[»]
ProteinModelPortaliP18912.
SMRiP18912.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18912.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 23Substrate bindingBy similarity3
Regioni59 – 62Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P18912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKTIRDVD VRGKRVFCRV DFNVPMEQGA ITDDTRIRAA LPTIRYLIEH
60 70 80 90 100
GAKVILASHL GRPKGKVVEE LRLDAVAKRL GELLERPVAK TNEAVGDEVK
110 120 130 140 150
AAVDRLNEGD VLLLENVRFY PGEEKNDPEL AKAFAELADL YVNDAFGAAH
160 170 180 190 200
RAHASTEGIA HYLPAVAGFL MEKELEVLGK ALSNPDRPFT AIIGGAKVKD
210 220 230 240 250
KIGVIDNLLE KVDNLIIGGG LAYTFVKALG HDVGKSLLEE DKIELAKSFM
260 270 280 290 300
EKAKEKGVRF YMPVDVVVAD RFANDANTKV VPIDAIPADW SALDIGPKTR
310 320 330 340 350
ELYRDVIRES KLVVWNGPMG VFEMDAFAHG TKAIAEALAE ALDTYSVIGG
360 370 380 390
GDSAAAVEKF GLADKMDHIS TGGGASLEFM EGKQLPGVVA LEDK
Length:394
Mass (Da):42,730
Last modified:August 1, 1992 - v2
Checksum:i46BFA286F60E365A
GO

Sequence cautioni

The sequence AAA22462 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58059 Genomic DNA. Translation: CAA41093.1.
M24493 Genomic DNA. Translation: AAA22462.1. Different initiation.
PIRiJQ1399.
RefSeqiWP_033015089.1. NZ_LUCR01000219.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58059 Genomic DNA. Translation: CAA41093.1.
M24493 Genomic DNA. Translation: AAA22462.1. Different initiation.
PIRiJQ1399.
RefSeqiWP_033015089.1. NZ_LUCR01000219.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PHPX-ray1.65A1-394[»]
ProteinModelPortaliP18912.
SMRiP18912.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BRENDAi2.7.2.3. 623.
SABIO-RKP18912.

Miscellaneous databases

EvolutionaryTraceiP18912.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_GEOSE
AccessioniPrimary (citable) accession number: P18912
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.