Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P18901 (DRD1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D(1A) dopamine receptor
Alternative name(s):
Dopamine D1 receptor
Gene names
Name:Drd1
Synonyms:Drd1a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Subunit structure

Interacts with calcyon By similarity. Interacts with DNAJC14 via its C-terminus. Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14. Ref.5

Tissue specificity

Brain, in the striatum, the nucleus accumbens, and the olfactory tubercle.

Post-translational modification

N-glycosylated. Ref.5

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Endoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactivation of adenylate cyclase activity

Inferred from direct assay PubMed 8702641. Source: BHF-UCL

adenylate cyclase-activating dopamine receptor signaling pathway

Inferred from direct assay PubMed 18424554. Source: BHF-UCL

associative learning

Inferred from mutant phenotype PubMed 15245880. Source: RGD

behavioral response to cocaine

Inferred from mutant phenotype PubMed 16377433. Source: RGD

calcium-mediated signaling

Inferred from direct assay PubMed 14983054. Source: RGD

cellular response to hypoxia

Inferred from expression pattern PubMed 20969567. Source: RGD

cellular response to insulin stimulus

Inferred from expression pattern PubMed 12707290. Source: RGD

dopamine receptor signaling pathway

Inferred from direct assay PubMed 17314300. Source: RGD

intracellular protein transport

Inferred from mutant phenotype PubMed 16641250. Source: RGD

locomotory behavior

Inferred from mutant phenotype PubMed 16377433. Source: RGD

negative regulation of cell migration

Inferred from mutant phenotype PubMed 12688394. Source: RGD

negative regulation of circadian sleep/wake cycle, sleep

Inferred from direct assay PubMed 12944496. Source: RGD

negative regulation of protein kinase activity

Inferred from mutant phenotype PubMed 12688394. Source: RGD

operant conditioning

Inferred from mutant phenotype PubMed 15245880. Source: RGD

orbitofrontal cortex development

Inferred from expression pattern PubMed 22015925. Source: RGD

phosphatidylinositol catabolic process

Inferred from direct assay PubMed 8702641. Source: BHF-UCL

phosphatidylinositol metabolic process

Inferred from direct assay PubMed 8848015. Source: BHF-UCL

positive regulation of adenylate cyclase activity

Inferred from direct assay PubMed 18424554. Source: BHF-UCL

positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

positive regulation of cAMP biosynthetic process

Inferred from direct assay PubMed 15351518. Source: RGD

positive regulation of feeding behavior

Inferred from mutant phenotype PubMed 11705777. Source: RGD

positive regulation of long-term synaptic potentiation

Inferred from mutant phenotype PubMed 20377617. Source: RGD

positive regulation of membrane potential

Inferred from mutant phenotype PubMed 18662324. Source: RGD

regulation of ion transport

Inferred from direct assay PubMed 14983054. Source: RGD

regulation of long-term neuronal synaptic plasticity

Inferred from mutant phenotype PubMed 16855100. Source: RGD

regulation of vasoconstriction

Inferred from mutant phenotype PubMed 15778266. Source: RGD

response to activity

Inferred from expression pattern PubMed 19759268. Source: RGD

response to amino acid

Inferred from expression pattern PubMed 17052844. Source: RGD

response to amphetamine

Inferred from expression pattern PubMed 19145071. Source: RGD

response to antidepressant

Inferred from expression pattern PubMed 16499906. Source: RGD

response to drug

Inferred from expression pattern PubMed 15798088. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 20534718. Source: RGD

response to ethanol

Inferred from expression pattern PubMed 23873704. Source: RGD

response to food

Inferred from expression pattern PubMed 20875839. Source: RGD

response to morphine

Inferred from expression pattern PubMed 23775491. Source: RGD

response to nicotine

Inferred from expression pattern PubMed 23447334. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 20673846. Source: RGD

response to organonitrogen compound

Inferred from expression pattern PubMed 23876469. Source: RGD

response to retinoic acid

Inferred from expression pattern PubMed 15939542. Source: RGD

response to steroid hormone

Inferred from expression pattern PubMed 18809391. Source: RGD

social behavior

Inferred from mutant phenotype PubMed 21557291. Source: RGD

striatum development

Inferred from expression pattern PubMed 22015925. Source: RGD

synapse assembly

Inferred from expression pattern PubMed 10051231. Source: BHF-UCL

synaptic transmission, dopaminergic

Inferred from direct assay PubMed 17314300. Source: RGD

vasodilation

Inferred from electronic annotation. Source: InterPro

visual learning

Inferred from mutant phenotype PubMed 16855100. Source: RGD

   Cellular_componentaxon

Inferred from direct assay PubMed 17576511. Source: RGD

axon terminus

Inferred from direct assay PubMed 17576511. Source: RGD

caveola

Inferred from direct assay PubMed 15265765. Source: RGD

dendrite

Inferred from direct assay PubMed 16641250. Source: RGD

dendritic shaft

Inferred from direct assay PubMed 16228995. Source: RGD

dendritic spine

Inferred from direct assay PubMed 11818555. Source: RGD

dendritic spine head

Inferred from direct assay PubMed 21809413. Source: RGD

dendritic spine neck

Inferred from direct assay PubMed 21809413. Source: RGD

endoplasmic reticulum

Inferred from direct assay PubMed 17576511. Source: RGD

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from direct assay PubMed 11818555. Source: RGD

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

neuronal cell body

Inferred from direct assay PubMed 17576511. Source: RGD

plasma membrane

Inferred from direct assay PubMed 18424554. Source: BHF-UCL

   Molecular_functionATPase binding

Inferred from physical interaction PubMed 20623535PubMed 21809413. Source: RGD

D3 dopamine receptor binding

Inferred from physical interaction PubMed 14732731. Source: RGD

G-protein alpha-subunit binding

Inferred from physical interaction PubMed 15798088. Source: RGD

G-protein coupled receptor activity

Inferred from direct assay PubMed 11818555. Source: RGD

angiotensin receptor binding

Inferred from physical interaction PubMed 22193384. Source: RGD

dopamine neurotransmitter receptor activity, coupled via Gs

Inferred from direct assay PubMed 11818555PubMed 17314300. Source: RGD

drug binding

Inferred from direct assay PubMed 16855100PubMed 17314300. Source: RGD

protein binding

Inferred from physical interaction PubMed 12408866. Source: UniProtKB

protein complex binding

Inferred from physical interaction PubMed 22843680. Source: RGD

protein heterodimerization activity

Inferred from physical interaction PubMed 22986162. Source: RGD

protein phosphatase binding

Inferred from physical interaction PubMed 23328768. Source: RGD

receptor binding

Inferred from physical interaction PubMed 12408866. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446D(1A) dopamine receptor
PRO_0000069378

Regions

Topological domain1 – 2222Extracellular Potential
Transmembrane23 – 4826Helical; Name=1; Potential
Topological domain49 – 5911Cytoplasmic Potential
Transmembrane60 – 8627Helical; Name=2; Potential
Topological domain87 – 959Extracellular Potential
Transmembrane96 – 11823Helical; Name=3; Potential
Topological domain119 – 13719Cytoplasmic Potential
Transmembrane138 – 16225Helical; Name=4; Potential
Topological domain163 – 19230Extracellular Potential
Transmembrane193 – 21826Helical; Name=5; Potential
Topological domain219 – 27254Cytoplasmic Potential
Transmembrane273 – 29927Helical; Name=6; Potential
Topological domain300 – 31213Extracellular Potential
Transmembrane313 – 33725Helical; Name=7; Potential
Topological domain338 – 446109Cytoplasmic Potential

Amino acid modifications

Lipidation3471S-palmitoyl cysteine By similarity
Lipidation3511S-palmitoyl cysteine By similarity
Glycosylation41N-linked (GlcNAc...) Potential
Disulfide bond95 ↔ 186 By similarity

Experimental info

Mutagenesis3331F → A: Loss of interaction with DNAJC14. Abolishes transport to the cell surface. Ref.5
Mutagenesis3371F → A: Loss of interaction with DNAJC14. Abolishes transport to the cell surface. Ref.5
Mutagenesis3411F → A: Loss of interaction with DNAJC14. Abolishes transport to the cell surface. Ref.5
Sequence conflict911L → F Ref.3
Sequence conflict931P → S Ref.3
Sequence conflict1651A → T Ref.2
Sequence conflict1901L → F Ref.2
Sequence conflict2271R → G Ref.2
Sequence conflict2531C → W Ref.2

Sequences

Sequence LengthMass (Da)Tools
P18901 [UniParc].

Last modified May 1, 1991. Version 2.
Checksum: 0380042E8590F470

FASTA44649,428
        10         20         30         40         50         60 
MAPNTSTMDE AGLPAERDFS FRILTACFLS LLILSTLLGN TLVCAAVIRF RHLRSKVTNF 

        70         80         90        100        110        120 
FVISLAVSDL LVAVLVMPWK AVAEIAGFWP LGPFCNIWVA FDIMCSTASI LNLCVISVDR 

       130        140        150        160        170        180 
YWAISSPFQY ERKMTPKAAF ILISVAWTLS VLISFIPVQL SWHKAKPTWP LDGNFTSLED 

       190        200        210        220        230        240 
TEDDNCDTRL SRTYAISSSL ISFYIPVAIM IVTYTSIYRI AQKQIRRISA LERAAVHAKN 

       250        260        270        280        290        300 
CQTTAGNGNP VECAQSESSF KMSFKRETKV LKTLSVIMGV FVCCWLPFFI SNCMVPFCGS 

       310        320        330        340        350        360 
EETQPFCIDS ITFDVFVWFG WANSSLNPII YAFNADFQKA FSTLLGCYRL CPTTNNAIET 

       370        380        390        400        410        420 
VSINNNGAVV FSSHHEPRGS ISKDCNLVYL IPHAVGSSED LKKEEAGGIA KPLEKLSPAL 

       430        440 
SVILDYDTDV SLEKIQPVTH SGQHST 

« Hide

References

[1]"Molecular cloning and expression of a D1 dopamine receptor linked to adenylyl cyclase activation."
Monsma F.J. Jr., Mahan L.C., McVittie L.D., Gerfen C.R., Sibley D.R.
Proc. Natl. Acad. Sci. U.S.A. 87:6723-6727(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[2]"Cloning and expression of human and rat D1 dopamine receptors."
Zhou Q.-Y., Grandy D.K., Thambi L., Kushner J.A., van Tol H.H.M., Cone R., Pribnow D., Salon J., Bunzow J.R., Civelli O.
Nature 347:76-80(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Characterization of gene organization and promoter region of the rat dopamine D1 receptor gene."
Zhou Q.Y., Li C., Civelli O.
J. Neurochem. 59:1875-1883(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Cloning of two additional catecholamine receptors from rat brain."
O'Dowd B.F., Nguyen T., Tirpak A., Jarvie K.R., Israel Y., Seeman P., Niznik H.B.
FEBS Lett. 262:8-12(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 114-263.
Tissue: Corpus striatum.
[5]"Regulation of transport of the dopamine D1 receptor by a new membrane-associated ER protein."
Bermak J.C., Li M., Bullock C.M., Zhou Q.-Y.
Nat. Cell Biol. 3:492-498(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DNAJC14, GLYCOSYLATION, MUTAGENESIS OF PHE-333; PHE-337 AND PHE-341, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35077 mRNA. Translation: AAA70428.1.
S46131 Genomic DNA. Translation: AAB23803.1.
PIRDYRTD1. A36049.
RefSeqNP_036678.2. NM_012546.2.
UniGeneRn.24039.

3D structure databases

ProteinModelPortalP18901.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid246496. 2 interactions.
DIPDIP-49024N.
STRING10116.ENSRNOP00000034820.

Chemistry

BindingDBP18901.
ChEMBLCHEMBL2096910.
GuidetoPHARMACOLOGY214.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP18901.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24316.
KEGGrno:24316.

Organism-specific databases

CTD1812.
RGD2518. Drd1.

Phylogenomic databases

eggNOGNOG262978.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidP18901.
KOK04144.
PhylomeDBP18901.

Gene expression databases

GenevestigatorP18901.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001413. Dopamine_D1_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00565. DOPAMINED1AR.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602967.
PROP18901.

Entry information

Entry nameDRD1_RAT
AccessionPrimary (citable) accession number: P18901
Secondary accession number(s): P21669
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 1, 1991
Last modified: June 11, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries