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P18899

- DDR48_YEAST

UniProt

P18899 - DDR48_YEAST

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Protein

Stress protein DDR48

Gene

DDR48

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA damage-responsive protein that may be required for maintaining the rate of spontaneous mutagenesis. Shows low ATP and GTP hydrolysis activity. Dispensable for acquisition of thermotolerance and does not play a significant role in recovery or protection of cells from acute heat shock.1 Publication

Kineticsi

  1. KM=0.29 mM for ATP1 Publication
  2. KM=0.58 mM for GTP1 Publication

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. GTPase activity Source: SGD

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. DNA repair Source: SGD
  3. GTP catabolic process Source: GOC
Complete GO annotation...

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-32861-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Stress protein DDR48
Alternative name(s):
DNA damage-responsive protein 48
Short name:
DDRP 48
Flocculent-specific protein
YP 75
Gene namesi
Name:DDR48
Synonyms:FSP
Ordered Locus Names:YMR173W
ORF Names:YM8010.03
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004784. DDR48.

Pathology & Biotechi

Disruption phenotypei

Shows a slightly altered sensitivity to killing by 4-nitroquinoline-1-oxide and to heat shock; and a 6- to 14-fold reduction of the spontaneous mutation rate of reversion of a HIS4 mutation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 430429Stress protein DDR48PRO_0000079849Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831Phosphoserine1 Publication
Modified residuei191 – 1911Phosphoserine1 Publication
Modified residuei314 – 3141Phosphoserine1 Publication
Modified residuei322 – 3221Phosphoserine1 Publication

Post-translational modificationi

Probably highly glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP18899.
PaxDbiP18899.
PeptideAtlasiP18899.

Expressioni

Inductioni

Expression is induced by DNA damage, as well as by salt, oxidative, and heat-shock stress.3 Publications

Gene expression databases

GenevestigatoriP18899.

Interactioni

Protein-protein interaction databases

BioGridi35351. 42 interactions.
DIPiDIP-4424N.
IntActiP18899. 3 interactions.
MINTiMINT-546743.
STRINGi4932.YMR173W.

Structurei

3D structure databases

ProteinModelPortaliP18899.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati74 – 8181
Repeati82 – 8982
Repeati90 – 9783
Repeati98 – 10584
Repeati106 – 11385
Repeati114 – 12186
Repeati124 – 13187; approximate
Repeati132 – 13988
Repeati141 – 14889
Repeati149 – 156810
Repeati157 – 164811
Repeati165 – 172812
Repeati175 – 182813; approximate
Repeati183 – 190814
Repeati191 – 198815
Repeati201 – 208816; approximate
Repeati209 – 216817
Repeati217 – 224818
Repeati225 – 232819
Repeati233 – 240820
Repeati243 – 250821; approximate
Repeati251 – 258822
Repeati260 – 267823
Repeati268 – 275824
Repeati278 – 285825; approximate
Repeati287 – 294826
Repeati296 – 303827
Repeati306 – 313828; approximate
Repeati314 – 321829
Repeati322 – 329830
Repeati332 – 339831; approximate
Repeati340 – 347832
Repeati348 – 355833
Repeati358 – 365834; approximate
Repeati366 – 373835
Repeati375 – 382836
Repeati393 – 400837; approximate
Repeati407 – 414838

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 41434138 X 8 AA approximate tandem repeats of S-[NS]-N-[ND]-D-S-Y-GAdd
BLAST

Sequence similaritiesi

Belongs to the DDR48 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG301978.
HOGENOMiHOG000000764.
InParanoidiP18899.
OMAiKNSYGDD.
OrthoDBiEOG70KH3D.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18899-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLFDKVKQF ANSNNNNNDS GNNNQGDYVT KAENMIGEDR VNQFKSKIGE
60 70 80 90 100
DRFDKMESKV RQQFSNTSIN DNDSNNNDSY GSNNNDSYGS NNNDSYGSNN
110 120 130 140 150
NDSYGSNNND SYGSNNDDSY GSSNKKKSSY GSNNDDSYGS SNNNDSYGSN
160 170 180 190 200
NNDSYGSNNN DSYGSNNDDS YGSSNKNKSS YGSNNDDSYG SNNDDSYGSS
210 220 230 240 250
NKKKSSYGSS NNDSYGSNND DSYGSNNNDS YGSNNDDSYG SSNKKKSSYG
260 270 280 290 300
SNNDDSYGSS NNNDSYGSNN DDSYGSSNKN KSSYGSSSND DSYGSSNNDD
310 320 330 340 350
SYGSSNKKKS SYGSNNDDSY GSNNDDSYGS SNKKKSSYGS SNNDSYGSNN
360 370 380 390 400
DDSYGSSNKK KSSYGSNNDD SYGSSNNNDS YGSNNDDSYG SSNRNKNSYG
410 420 430
SSNYGSSNND DSYGSSNRGG RNQYGGDDDY
Length:430
Mass (Da):46,233
Last modified:January 23, 2007 - v4
Checksum:i650212489A8828AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91Q → E AA sequence (PubMed:2114092)Curated
Sequence conflicti38 – 381E → Q in AAA34563. (PubMed:2111448)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36110 Genomic DNA. Translation: AAA34563.1.
S73336 Genomic DNA. Translation: AAB31954.1.
Z49808 Genomic DNA. Translation: CAA89906.1.
BK006946 Genomic DNA. Translation: DAA10069.1.
PIRiS55120. HHBYD8.
RefSeqiNP_013897.1. NM_001182678.1.

Genome annotation databases

EnsemblFungiiYMR173W; YMR173W; YMR173W.
GeneIDi855210.
KEGGisce:YMR173W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36110 Genomic DNA. Translation: AAA34563.1 .
S73336 Genomic DNA. Translation: AAB31954.1 .
Z49808 Genomic DNA. Translation: CAA89906.1 .
BK006946 Genomic DNA. Translation: DAA10069.1 .
PIRi S55120. HHBYD8.
RefSeqi NP_013897.1. NM_001182678.1.

3D structure databases

ProteinModelPortali P18899.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35351. 42 interactions.
DIPi DIP-4424N.
IntActi P18899. 3 interactions.
MINTi MINT-546743.
STRINGi 4932.YMR173W.

Proteomic databases

MaxQBi P18899.
PaxDbi P18899.
PeptideAtlasi P18899.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR173W ; YMR173W ; YMR173W .
GeneIDi 855210.
KEGGi sce:YMR173W.

Organism-specific databases

SGDi S000004784. DDR48.

Phylogenomic databases

eggNOGi NOG301978.
HOGENOMi HOG000000764.
InParanoidi P18899.
OMAi KNSYGDD.
OrthoDBi EOG70KH3D.

Enzyme and pathway databases

BioCyci YEAST:G3O-32861-MONOMER.

Miscellaneous databases

NextBioi 978711.
PROi P18899.

Gene expression databases

Genevestigatori P18899.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the DNA damage-inducible gene DDR48 and evidence for its role in mutagenesis in Saccharomyces cerevisiae."
    Treger J.M., McEntee K.
    Mol. Cell. Biol. 10:3174-3184(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
  2. "Molecular cloning of the gene encoding a highly expressed protein in SFL1 gene-disrupted flocculating yeast."
    Tonouchi A., Fujita A., Kuhara S.
    J. Biochem. 115:683-688(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Purification and characterization of Saccharomyces cerevisiae DNA damage-responsive protein 48 (DDRP 48)."
    Sheng S., Schuster S.M.
    J. Biol. Chem. 268:4752-4758(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-31, CLEAVAGE OF INITIATOR METHIONINE, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION, INDUCTION.
  6. "Purification, characterization and partial amino acid sequence of glycogen synthase from Saccharomyces cerevisiae."
    Carabaza A., Arino J., Fox J.W., Villar-Palasi C., Guinovart J.J.
    Biochem. J. 268:401-407(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12.
  7. "DNA damage and heat shock dually regulate genes in Saccharomyces cerevisiae."
    McClanahan T., McEntee K.
    Mol. Cell. Biol. 6:90-96(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-183; SER-191; SER-314 AND SER-322, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Yeast adapts to a changing stressful environment by evolving cross-protection and anticipatory gene regulation."
    Dhar R., Sagesser R., Weikert C., Wagner A.
    Mol. Biol. Evol. 30:573-588(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiDDR48_YEAST
AccessioniPrimary (citable) accession number: P18899
Secondary accession number(s): D6VZZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 116 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

sequence is a contaminating peptide from a S.cerevisiae glycogen synthase purification.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3