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Protein

Stress protein DDR48

Gene

DDR48

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA damage-responsive protein that may be required for maintaining the rate of spontaneous mutagenesis. Shows low ATP and GTP hydrolysis activity. Dispensable for acquisition of thermotolerance and does not play a significant role in recovery or protection of cells from acute heat shock.1 Publication

Kineticsi

  1. KM=0.29 mM for ATP1 Publication
  2. KM=0.58 mM for GTP1 Publication

    GO - Molecular functioni

    • ATPase activity Source: SGD
    • GTPase activity Source: SGD

    GO - Biological processi

    • cellular response to oxidative stress Source: GO_Central
    • DNA repair Source: SGD
    Complete GO annotation...

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32861-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Stress protein DDR48
    Alternative name(s):
    DNA damage-responsive protein 48
    Short name:
    DDRP 48
    Flocculent-specific protein
    YP 75
    Gene namesi
    Name:DDR48
    Synonyms:FSP
    Ordered Locus Names:YMR173W
    ORF Names:YM8010.03
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIII

    Organism-specific databases

    EuPathDBiFungiDB:YMR173W.
    SGDiS000004784. DDR48.

    Pathology & Biotechi

    Disruption phenotypei

    Shows a slightly altered sensitivity to killing by 4-nitroquinoline-1-oxide and to heat shock; and a 6- to 14-fold reduction of the spontaneous mutation rate of reversion of a HIS4 mutation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00000798492 – 430Stress protein DDR48Add BLAST429

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei183PhosphoserineCombined sources1
    Modified residuei191PhosphoserineCombined sources1
    Modified residuei314PhosphoserineCombined sources1
    Modified residuei322PhosphoserineCombined sources1

    Post-translational modificationi

    Probably highly glycosylated.1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP18899.
    PRIDEiP18899.
    TopDownProteomicsiP18899.

    PTM databases

    iPTMnetiP18899.

    Expressioni

    Inductioni

    Expression is induced by DNA damage, as well as by salt, oxidative, and heat-shock stress.3 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi35351. 42 interactors.
    DIPiDIP-4424N.
    IntActiP18899. 4 interactors.
    MINTiMINT-546743.

    Structurei

    3D structure databases

    ProteinModelPortaliP18899.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati74 – 8118
    Repeati82 – 8928
    Repeati90 – 9738
    Repeati98 – 10548
    Repeati106 – 11358
    Repeati114 – 12168
    Repeati124 – 1317; approximate8
    Repeati132 – 13988
    Repeati141 – 14898
    Repeati149 – 156108
    Repeati157 – 164118
    Repeati165 – 172128
    Repeati175 – 18213; approximate8
    Repeati183 – 190148
    Repeati191 – 198158
    Repeati201 – 20816; approximate8
    Repeati209 – 216178
    Repeati217 – 224188
    Repeati225 – 232198
    Repeati233 – 240208
    Repeati243 – 25021; approximate8
    Repeati251 – 258228
    Repeati260 – 267238
    Repeati268 – 275248
    Repeati278 – 28525; approximate8
    Repeati287 – 294268
    Repeati296 – 303278
    Repeati306 – 31328; approximate8
    Repeati314 – 321298
    Repeati322 – 329308
    Repeati332 – 33931; approximate8
    Repeati340 – 347328
    Repeati348 – 355338
    Repeati358 – 36534; approximate8
    Repeati366 – 373358
    Repeati375 – 382368
    Repeati393 – 40037; approximate8
    Repeati407 – 414388

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni74 – 41438 X 8 AA approximate tandem repeats of S-[NS]-N-[ND]-D-S-Y-GAdd BLAST341

    Sequence similaritiesi

    Belongs to the DDR48 family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    GeneTreeiENSGT00840000131180.
    HOGENOMiHOG000000764.
    InParanoidiP18899.
    OMAiDRDTYGS.
    OrthoDBiEOG092C6092.

    Family and domain databases

    InterProiIPR033208. Ddr48.
    [Graphical view]
    PANTHERiPTHR35003:SF2. PTHR35003:SF2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18899-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGLFDKVKQF ANSNNNNNDS GNNNQGDYVT KAENMIGEDR VNQFKSKIGE
    60 70 80 90 100
    DRFDKMESKV RQQFSNTSIN DNDSNNNDSY GSNNNDSYGS NNNDSYGSNN
    110 120 130 140 150
    NDSYGSNNND SYGSNNDDSY GSSNKKKSSY GSNNDDSYGS SNNNDSYGSN
    160 170 180 190 200
    NNDSYGSNNN DSYGSNNDDS YGSSNKNKSS YGSNNDDSYG SNNDDSYGSS
    210 220 230 240 250
    NKKKSSYGSS NNDSYGSNND DSYGSNNNDS YGSNNDDSYG SSNKKKSSYG
    260 270 280 290 300
    SNNDDSYGSS NNNDSYGSNN DDSYGSSNKN KSSYGSSSND DSYGSSNNDD
    310 320 330 340 350
    SYGSSNKKKS SYGSNNDDSY GSNNDDSYGS SNKKKSSYGS SNNDSYGSNN
    360 370 380 390 400
    DDSYGSSNKK KSSYGSNNDD SYGSSNNNDS YGSNNDDSYG SSNRNKNSYG
    410 420 430
    SSNYGSSNND DSYGSSNRGG RNQYGGDDDY
    Length:430
    Mass (Da):46,233
    Last modified:January 23, 2007 - v4
    Checksum:i650212489A8828AC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti9Q → E AA sequence (PubMed:2114092).Curated1
    Sequence conflicti38E → Q in AAA34563 (PubMed:2111448).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M36110 Genomic DNA. Translation: AAA34563.1.
    S73336 Genomic DNA. Translation: AAB31954.1.
    Z49808 Genomic DNA. Translation: CAA89906.1.
    BK006946 Genomic DNA. Translation: DAA10069.1.
    PIRiS55120. HHBYD8.
    RefSeqiNP_013897.1. NM_001182678.1.

    Genome annotation databases

    EnsemblFungiiYMR173W; YMR173W; YMR173W.
    GeneIDi855210.
    KEGGisce:YMR173W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M36110 Genomic DNA. Translation: AAA34563.1.
    S73336 Genomic DNA. Translation: AAB31954.1.
    Z49808 Genomic DNA. Translation: CAA89906.1.
    BK006946 Genomic DNA. Translation: DAA10069.1.
    PIRiS55120. HHBYD8.
    RefSeqiNP_013897.1. NM_001182678.1.

    3D structure databases

    ProteinModelPortaliP18899.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35351. 42 interactors.
    DIPiDIP-4424N.
    IntActiP18899. 4 interactors.
    MINTiMINT-546743.

    PTM databases

    iPTMnetiP18899.

    Proteomic databases

    MaxQBiP18899.
    PRIDEiP18899.
    TopDownProteomicsiP18899.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYMR173W; YMR173W; YMR173W.
    GeneIDi855210.
    KEGGisce:YMR173W.

    Organism-specific databases

    EuPathDBiFungiDB:YMR173W.
    SGDiS000004784. DDR48.

    Phylogenomic databases

    GeneTreeiENSGT00840000131180.
    HOGENOMiHOG000000764.
    InParanoidiP18899.
    OMAiDRDTYGS.
    OrthoDBiEOG092C6092.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32861-MONOMER.

    Miscellaneous databases

    PROiP18899.

    Family and domain databases

    InterProiIPR033208. Ddr48.
    [Graphical view]
    PANTHERiPTHR35003:SF2. PTHR35003:SF2. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDDR48_YEAST
    AccessioniPrimary (citable) accession number: P18899
    Secondary accession number(s): D6VZZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 134 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    sequence is a contaminating peptide from a S.cerevisiae glycogen synthase purification.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.