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Protein

Geranylgeranyl transferase type-1 subunit beta

Gene

CDC43

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to proteins having the C-terminal sequence Cys-Ile-Ile-Leu or Cys-Val-Leu-Leu. Acts, among other substrates, on Rho1 and Rho2 and CDC42 proteins. Participates in a RAS-like C-terminal modification of proteins involved in nuclear division and bud growth. It is involved in bud positioning and cell polarity.1 Publication

Catalytic activityi

Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861Zinc; catalyticBy similarity
Metal bindingi288 – 2881Zinc; catalyticBy similarity
Metal bindingi341 – 3411Zinc; via tele nitrogen; catalyticBy similarity

GO - Molecular functioni

  • CAAX-protein geranylgeranyltransferase activity Source: SGD
  • protein geranylgeranyltransferase activity Source: SGD
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cellular calcium ion homeostasis Source: SGD
  • establishment of cell polarity Source: SGD
  • protein geranylgeranylation Source: SGD
  • small GTPase mediated signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Prenyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YGL155W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Geranylgeranyl transferase type-1 subunit beta (EC:2.5.1.59)
Alternative name(s):
Cell division cycle protein 43
Geranylgeranyl transferase type I subunit beta
Short name:
GGTase-I-beta
RAS proteins geranylgeranyltransferase subunit beta
Type I protein geranyl-geranyltransferase subunit beta
Short name:
PGGT
Gene namesi
Name:CDC43
Synonyms:CAL1
Ordered Locus Names:YGL155W
ORF Names:G1864
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL155W.
SGDiS000003123. CDC43.

Subcellular locationi

GO - Cellular componenti

  • CAAX-protein geranylgeranyltransferase complex Source: SGD
  • intracellular Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi328 – 3281G → S: Defect in nuclear division and bud formation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Geranylgeranyl transferase type-1 subunit betaPRO_0000119775Add
BLAST

Proteomic databases

MaxQBiP18898.
PeptideAtlasiP18898.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
RAM2P297033EBI-3961,EBI-14814

Protein-protein interaction databases

BioGridi33099. 47 interactions.
DIPiDIP-1232N.
IntActiP18898. 1 interaction.
MINTiMINT-396220.

Structurei

3D structure databases

ProteinModelPortaliP18898.
SMRiP18898. Positions 15-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati128 – 17952PFTB 1Add
BLAST
Repeati192 – 23140PFTB 2Add
BLAST
Repeati259 – 30143PFTB 3Add
BLAST
Repeati310 – 35344PFTB 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni216 – 2183Geranylgeranyl diphosphate bindingBy similarity
Regioni280 – 2834Geranylgeranyl diphosphate bindingBy similarity
Regioni289 – 2924Geranylgeranyl diphosphate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi138 – 18043Cys-richAdd
BLAST

Sequence similaritiesi

Contains 4 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000180333.
InParanoidiP18898.
KOiK11713.
OMAiIYFCLHG.
OrthoDBiEOG74N5SC.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. PFTB_repeat.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 4 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.

Sequencei

Sequence statusi: Complete.

P18898-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCQATNGPSR VVTKKHRKFF ERHLQLLPSS HQGHDVNRMA IIFYSISGLS
60 70 80 90 100
IFDVNVSAKY GDHLGWMRKH YIKTVLDDTE NTVISGFVGS LVMNIPHATT
110 120 130 140 150
INLPNTLFAL LSMIMLRDYE YFETILDKRS LARFVSKCQR PDRGSFVSCL
160 170 180 190 200
DYKTNCGSSV DSDDLRFCYI AVAILYICGC RSKEDFDEYI DTEKLLGYIM
210 220 230 240 250
SQQCYNGAFG AHNEPHSGYT SCALSTLALL SSLEKLSDKF KEDTITWLLH
260 270 280 290 300
RQVSSHGCMK FESELNASYD QSDDGGFQGR ENKFADTCYA FWCLNSLHLL
310 320 330 340 350
TKDWKMLCQT ELVTNYLLDR TQKTLTGGFS KNDEEDADLY HSCLGSAALA
360 370
LIEGKFNGEL CIPQEIFNDF SKRCCF
Length:376
Mass (Da):42,690
Last modified:April 1, 1993 - v2
Checksum:iB52E56F353B3914E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31114 Genomic DNA. Translation: AAA34481.1.
M74109 Genomic DNA. Translation: AAA34464.1.
Z48618 Genomic DNA. No translation available.
Z72677 Genomic DNA. Translation: CAA96867.1.
BK006941 Genomic DNA. Translation: DAA07957.1.
PIRiA40875. RGBY43.
RefSeqiNP_011360.1. NM_001181020.1.

Genome annotation databases

EnsemblFungiiYGL155W; YGL155W; YGL155W.
GeneIDi852722.
KEGGisce:YGL155W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31114 Genomic DNA. Translation: AAA34481.1.
M74109 Genomic DNA. Translation: AAA34464.1.
Z48618 Genomic DNA. No translation available.
Z72677 Genomic DNA. Translation: CAA96867.1.
BK006941 Genomic DNA. Translation: DAA07957.1.
PIRiA40875. RGBY43.
RefSeqiNP_011360.1. NM_001181020.1.

3D structure databases

ProteinModelPortaliP18898.
SMRiP18898. Positions 15-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33099. 47 interactions.
DIPiDIP-1232N.
IntActiP18898. 1 interaction.
MINTiMINT-396220.

Proteomic databases

MaxQBiP18898.
PeptideAtlasiP18898.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL155W; YGL155W; YGL155W.
GeneIDi852722.
KEGGisce:YGL155W.

Organism-specific databases

EuPathDBiFungiDB:YGL155W.
SGDiS000003123. CDC43.

Phylogenomic databases

HOGENOMiHOG000180333.
InParanoidiP18898.
KOiK11713.
OMAiIYFCLHG.
OrthoDBiEOG74N5SC.

Enzyme and pathway databases

BioCyciYEAST:YGL155W-MONOMER.

Miscellaneous databases

NextBioi972105.
PROiP18898.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
InterProiIPR001330. PFTB_repeat.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF00432. Prenyltrans. 4 hits.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and sequence analysis of CDC43, a gene involved in the control of cell polarity in Saccharomyces cerevisiae."
    Johnson D.I., O'Brien J.M., Jacobs C.W.
    Gene 90:93-98(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation and sequence analysis of CDC43, a gene involved in the control of cell polarity in Saccharomyces cerevisiae."
    Johnson D.I., O'Brien J.M., Jacobs C.W.
    Gene 98:149-150(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Yeast CAL1 is a structural and functional homologue to the DPR1 (RAM) gene involved in ras processing."
    Ohya Y., Goebl M., Goodman L.E., Petersen-Bjoern S., Friesen J.D., Tamanoi F., Anraku Y.
    J. Biol. Chem. 266:12356-12360(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A5-8-1A.
  4. "DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43."
    James C.M., Indge K.J., Oliver S.G.
    Yeast 11:1413-1419(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Protein geranylgeranyltransferase of Saccharomyces cerevisiae is specific for Cys-Xaa-Xaa-Leu motif proteins and requires the CDC43 gene product but not the DPR1 gene product."
    Finegold A.A., Johnson D.I., Farnsworth C.C., Gelb M.H., Judd S.R., Glomset J.A., Tamanoi F.
    Proc. Natl. Acad. Sci. U.S.A. 88:4448-4452(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPGTB1_YEAST
AccessioniPrimary (citable) accession number: P18898
Secondary accession number(s): D6VTZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 1, 1993
Last modified: May 11, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.