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P18895

- ALGE_PSEAE

UniProt

P18895 - ALGE_PSEAE

Protein

Alginate production protein AlgE

Gene

algE

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (08 Dec 2000)
      Previous versions | rss
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    Functioni

    Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.

    Pathwayi

    GO - Biological processi

    1. alginic acid biosynthetic process Source: PseudoCAP

    Keywords - Biological processi

    Alginate biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00286.

    Protein family/group databases

    TCDBi1.B.13.1.1. the alginate export porin (aep) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alginate production protein AlgE
    Gene namesi
    Name:algE
    Synonyms:alg76
    Ordered Locus Names:PA3544
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA3544.

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 32321 PublicationAdd
    BLAST
    Chaini33 – 490458Alginate production protein AlgEPRO_0000020663Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA3544.

    Structurei

    Secondary structure

    1
    490
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi42 – 5413
    Turni56 – 594
    Beta strandi60 – 634
    Beta strandi66 – 8116
    Beta strandi84 – 9714
    Helixi117 – 1193
    Beta strandi123 – 13513
    Beta strandi138 – 1403
    Beta strandi144 – 15411
    Beta strandi158 – 1603
    Beta strandi162 – 17312
    Beta strandi175 – 18511
    Beta strandi191 – 1933
    Turni199 – 2035
    Beta strandi204 – 21613
    Beta strandi219 – 23012
    Beta strandi247 – 26216
    Beta strandi270 – 29526
    Beta strandi297 – 31923
    Beta strandi321 – 33313
    Helixi338 – 3403
    Beta strandi358 – 3614
    Turni368 – 3703
    Beta strandi374 – 38815
    Turni389 – 3913
    Beta strandi392 – 40514
    Beta strandi411 – 4144
    Beta strandi425 – 43713
    Beta strandi439 – 4413
    Helixi448 – 4514
    Beta strandi457 – 46711
    Turni469 – 4713
    Beta strandi479 – 48911

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RBHX-ray2.30A/B/C/D33-490[»]
    4AFKX-ray1.90A33-490[»]
    4AZLX-ray2.80A/B33-490[»]
    4B61X-ray2.40A/B33-490[»]
    ProteinModelPortaliP18895.
    SMRiP18895. Positions 38-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP18895.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AlgE family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44459.
    HOGENOMiHOG000247758.
    KOiK16081.
    OMAiHRFGEAF.
    OrthoDBiEOG6NWBKV.

    Family and domain databases

    InterProiIPR025388. Alginate_export_dom.
    [Graphical view]
    PfamiPF13372. Alginate_exp. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18895-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNSSRSVNPR PSFAPRALSL AIALLLGAPA FAANSGEAPK NFGLDVKITG    50
    ESENDRDLGT APGGTLNDIG IDLRPWAFGQ WGDWSAYFMG QAVAATDTIE 100
    TDTLQSDTDD GNNSRNDGRE PDKSYLAARE FWVDYAGLTA YPGEHLRFGR 150
    QRLREDSGQW QDTNIEALNW SFETTLLNAH AGVAQRFSEY RTDLDELAPE 200
    DKDRTHVFGD ISTQWAPHHR IGVRIHHADD SGHLRRPGEE VDNLDKTYTG 250
    QLTWLGIEAT GDAYNYRSSM PLNYWASATW LTGDRDNLTT TTVDDRRIAT 300
    GKQSGDVNAF GVDLGLRWNI DEQWKAGVGY ARGSGGGKDG EEQFQQTGLE 350
    SNRSNFTGTR SRVHRFGEAF RGELSNLQAA TLFGSWQLRE DYDASLVYHK 400
    FWRVDDDSDI GTSGINAALQ PGEKDIGQEL DLVVTKYFKQ GLLPASMSQY 450
    VDEPSALIRF RGGLFKPGDA YGPGTDSTMH RAFVDFIWRF 490
    Length:490
    Mass (Da):54,422
    Last modified:December 8, 2000 - v2
    Checksum:i13B7A9627CE36C8C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341N → G in AAA25699. (PubMed:1743507)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37181 Genomic DNA. Translation: AAA25699.1.
    AE004091 Genomic DNA. Translation: AAG06932.1.
    PIRiE83202.
    JS0586.
    RefSeqiNP_252234.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06932; AAG06932; PA3544.
    GeneIDi879838.
    KEGGipae:PA3544.
    PATRICi19841701. VBIPseAer58763_3708.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M37181 Genomic DNA. Translation: AAA25699.1 .
    AE004091 Genomic DNA. Translation: AAG06932.1 .
    PIRi E83202.
    JS0586.
    RefSeqi NP_252234.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RBH X-ray 2.30 A/B/C/D 33-490 [» ]
    4AFK X-ray 1.90 A 33-490 [» ]
    4AZL X-ray 2.80 A/B 33-490 [» ]
    4B61 X-ray 2.40 A/B 33-490 [» ]
    ProteinModelPortali P18895.
    SMRi P18895. Positions 38-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA3544.

    Protein family/group databases

    TCDBi 1.B.13.1.1. the alginate export porin (aep) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06932 ; AAG06932 ; PA3544 .
    GeneIDi 879838.
    KEGGi pae:PA3544.
    PATRICi 19841701. VBIPseAer58763_3708.

    Organism-specific databases

    PseudoCAPi PA3544.

    Phylogenomic databases

    eggNOGi NOG44459.
    HOGENOMi HOG000247758.
    KOi K16081.
    OMAi HRFGEAF.
    OrthoDBi EOG6NWBKV.

    Enzyme and pathway databases

    UniPathwayi UPA00286 .

    Miscellaneous databases

    EvolutionaryTracei P18895.

    Family and domain databases

    InterProi IPR025388. Alginate_export_dom.
    [Graphical view ]
    Pfami PF13372. Alginate_exp. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and expression of the algE gene involved in alginate biosynthesis by Pseudomonas aeruginosa."
      Chu L., May T.B., Chakrabarty A.M., Misra T.K.
      Gene 107:1-10(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 33-42.
      Strain: 8830.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    3. "Overexpression of algE in Escherichia coli: subcellular localization, purification, and ion channel properties."
      Rehm B.H., Boheim G., Tommassen J., Winkler U.K.
      J. Bacteriol. 176:5639-5647(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: CF3/M1.

    Entry informationi

    Entry nameiALGE_PSEAE
    AccessioniPrimary (citable) accession number: P18895
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 8, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3