P18895 (ALGE_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate production protein AlgE

Gene names

Name:	algE
Synonyms:	alg76
Ordered Locus Names:	PA3544

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.
Pathway	Glycan biosynthesis; alginate biosynthesis.
Subcellular location	Cell outer membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the AlgE family.

Ontologies

Keywords
Biological process	Alginate biosynthesis
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	alginic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 32

32

Ref.1

Chain

33 – 490

458

Alginate production protein AlgE

PRO_0000020663

Experimental info

Sequence conflict

34

1

N → G in AAA25699. Ref.1

Secondary structure

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490

Helix Strand Turn

Details...

Beta strand

42 – 54

13

Turn

56 – 59

4

Beta strand

60 – 63

4

Beta strand

66 – 81

16

Beta strand

84 – 97

14

Beta strand

123 – 135

13

Beta strand

138 – 140

3

Beta strand

144 – 154

11

Beta strand

158 – 160

3

Beta strand

162 – 173

12

Beta strand

175 – 185

11

Beta strand

191 – 193

3

Turn

199 – 203

5

Beta strand

204 – 216

13

Beta strand

219 – 230

12

Beta strand

247 – 262

16

Beta strand

270 – 295

26

Beta strand

297 – 319

23

Beta strand

321 – 333

13

Helix

338 – 340

3

Beta strand

358 – 361

4

Turn

368 – 370

3

Beta strand

374 – 388

15

Turn

389 – 391

3

Beta strand

392 – 405

14

Beta strand

425 – 437

13

Beta strand

439 – 441

3

Helix

448 – 451

4

Beta strand

457 – 467

11

Turn

469 – 471

3

Beta strand

479 – 489

11

Sequences

	Sequence		Length	Mass (Da)
	P18895 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 13B7A9627CE36C8C	FASTA	490	54,422
	10 20 30 40 50 60 MNSSRSVNPR PSFAPRALSL AIALLLGAPA FAANSGEAPK NFGLDVKITG ESENDRDLGT 70 80 90 100 110 120 APGGTLNDIG IDLRPWAFGQ WGDWSAYFMG QAVAATDTIE TDTLQSDTDD GNNSRNDGRE 130 140 150 160 170 180 PDKSYLAARE FWVDYAGLTA YPGEHLRFGR QRLREDSGQW QDTNIEALNW SFETTLLNAH 190 200 210 220 230 240 AGVAQRFSEY RTDLDELAPE DKDRTHVFGD ISTQWAPHHR IGVRIHHADD SGHLRRPGEE 250 260 270 280 290 300 VDNLDKTYTG QLTWLGIEAT GDAYNYRSSM PLNYWASATW LTGDRDNLTT TTVDDRRIAT 310 320 330 340 350 360 GKQSGDVNAF GVDLGLRWNI DEQWKAGVGY ARGSGGGKDG EEQFQQTGLE SNRSNFTGTR 370 380 390 400 410 420 SRVHRFGEAF RGELSNLQAA TLFGSWQLRE DYDASLVYHK FWRVDDDSDI GTSGINAALQ 430 440 450 460 470 480 PGEKDIGQEL DLVVTKYFKQ GLLPASMSQY VDEPSALIRF RGGLFKPGDA YGPGTDSTMH 490 RAFVDFIWRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression of the algE gene involved in alginate biosynthesis by Pseudomonas aeruginosa." Chu L., May T.B., Chakrabarty A.M., Misra T.K. Gene 107:1-10(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 33-42. Strain: 8830.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Overexpression of algE in Escherichia coli: subcellular localization, purification, and ion channel properties." Rehm B.H., Boheim G., Tommassen J., Winkler U.K. J. Bacteriol. 176:5639-5647(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: CF3/M1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M37181 Genomic DNA. Translation: AAA25699.1.
AE004091 Genomic DNA. Translation: AAG06932.1.

PIR

E83202.
JS0586.

RefSeq

NP_252234.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RBH	X-ray	2.30	A/B/C/D	33-490	[»]
4AFK	X-ray	1.90	A	33-490	[»]

ProteinModelPortal

P18895.

SMR

P18895. Positions 40-490.

ModBase

Search...

Protein-protein interaction databases

STRING

208964.PA3544.

Protein family/group databases

TCDB

1.B.13.1.1. alginate export porin (AEP) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

879838.

KEGG

pae:PA3544.

PATRIC

19841701. VBIPseAer58763_3708.

Organism-specific databases

PseudoCAP

PA3544.

Phylogenomic databases

eggNOG

NOG44459.

HOGENOM

HOG000247758.

KO

K16081.

OMA

HRFGEAF.

ProtClustDB

CLSK868060.

Enzyme and pathway databases

UniPathway

UPA00286.

Family and domain databases

ProtoNet

Search...

Other

EvolutionaryTrace

P18895.

Entry information

Entry name

ALGE_PSEAE

Accession

Primary (citable) accession number: P18895

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1990
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program