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P18895 (ALGE_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alginate production protein AlgE
Gene names
Name:algE
Synonyms:alg76
Ordered Locus Names:PA3544
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore.

Pathway

Glycan biosynthesis; alginate biosynthesis.

Subcellular location

Cell outer membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the AlgE family.

Ontologies

Keywords
   Biological processAlginate biosynthesis
   Cellular componentCell outer membrane
Membrane
   DomainSignal
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processalginic acid biosynthetic process

Inferred from mutant phenotype PubMed 23335756. Source: PseudoCAP

   Cellular_componentcell outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Ref.1
Chain33 – 490458Alginate production protein AlgE
PRO_0000020663

Experimental info

Sequence conflict341N → G in AAA25699. Ref.1

Secondary structure

............................................................... 490
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P18895 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 13B7A9627CE36C8C

FASTA49054,422
        10         20         30         40         50         60 
MNSSRSVNPR PSFAPRALSL AIALLLGAPA FAANSGEAPK NFGLDVKITG ESENDRDLGT 

        70         80         90        100        110        120 
APGGTLNDIG IDLRPWAFGQ WGDWSAYFMG QAVAATDTIE TDTLQSDTDD GNNSRNDGRE 

       130        140        150        160        170        180 
PDKSYLAARE FWVDYAGLTA YPGEHLRFGR QRLREDSGQW QDTNIEALNW SFETTLLNAH 

       190        200        210        220        230        240 
AGVAQRFSEY RTDLDELAPE DKDRTHVFGD ISTQWAPHHR IGVRIHHADD SGHLRRPGEE 

       250        260        270        280        290        300 
VDNLDKTYTG QLTWLGIEAT GDAYNYRSSM PLNYWASATW LTGDRDNLTT TTVDDRRIAT 

       310        320        330        340        350        360 
GKQSGDVNAF GVDLGLRWNI DEQWKAGVGY ARGSGGGKDG EEQFQQTGLE SNRSNFTGTR 

       370        380        390        400        410        420 
SRVHRFGEAF RGELSNLQAA TLFGSWQLRE DYDASLVYHK FWRVDDDSDI GTSGINAALQ 

       430        440        450        460        470        480 
PGEKDIGQEL DLVVTKYFKQ GLLPASMSQY VDEPSALIRF RGGLFKPGDA YGPGTDSTMH 

       490 
RAFVDFIWRF 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and expression of the algE gene involved in alginate biosynthesis by Pseudomonas aeruginosa."
Chu L., May T.B., Chakrabarty A.M., Misra T.K.
Gene 107:1-10(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 33-42.
Strain: 8830.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"Overexpression of algE in Escherichia coli: subcellular localization, purification, and ion channel properties."
Rehm B.H., Boheim G., Tommassen J., Winkler U.K.
J. Bacteriol. 176:5639-5647(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: CF3/M1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M37181 Genomic DNA. Translation: AAA25699.1.
AE004091 Genomic DNA. Translation: AAG06932.1.
PIRE83202.
JS0586.
RefSeqNP_252234.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RBHX-ray2.30A/B/C/D33-490[»]
4AFKX-ray1.90A33-490[»]
4AZLX-ray2.80A/B33-490[»]
4B61X-ray2.40A/B33-490[»]
ProteinModelPortalP18895.
SMRP18895. Positions 38-490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA3544.

Protein family/group databases

TCDB1.B.13.1.1. the alginate export porin (aep) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID879838.
KEGGpae:PA3544.
PATRIC19841701. VBIPseAer58763_3708.

Organism-specific databases

PseudoCAPPA3544.

Phylogenomic databases

eggNOGNOG44459.
HOGENOMHOG000247758.
KOK16081.
OMAHRFGEAF.
OrthoDBEOG6NWBKV.
ProtClustDBCLSK868060.

Enzyme and pathway databases

UniPathwayUPA00286.

Family and domain databases

InterProIPR025388. Alginate_export_dom.
[Graphical view]
PfamPF13372. Alginate_exp. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP18895.

Entry information

Entry nameALGE_PSEAE
AccessionPrimary (citable) accession number: P18895
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 8, 2000
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways