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Protein

D-amino-acid oxidase

Gene

Dao

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.

Catalytic activityi

A D-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52SubstrateBy similarity1
Binding sitei163FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei180FADBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei226SubstrateBy similarity1
Binding sitei281SubstrateBy similarity1
Binding sitei311Substrate; via carbonyl oxygenBy similarity1
Binding sitei315FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi3 – 17FADBy similarityAdd BLAST15
Nucleotide bindingi36 – 37FADBy similarity2
Nucleotide bindingi43 – 44FADBy similarity2
Nucleotide bindingi48 – 50FADBy similarity3
Nucleotide bindingi310 – 314FADBy similarity5

GO - Molecular functioni

  • cofactor binding Source: UniProtKB
  • D-amino-acid oxidase activity Source: UniProtKB
  • FAD binding Source: MGI
  • protein dimerization activity Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

  • D-alanine catabolic process Source: UniProtKB
  • dopamine biosynthetic process Source: MGI
  • D-serine catabolic process Source: MGI
  • D-serine metabolic process Source: MGI
  • leucine metabolic process Source: MGI
  • proline catabolic process Source: MGI

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.4.3.3 3474

Names & Taxonomyi

Protein namesi
Recommended name:
D-amino-acid oxidase (EC:1.4.3.3)
Short name:
DAAO
Short name:
DAMOX
Short name:
DAO
Gene namesi
Name:Dao
Synonyms:Dao1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:94859 Dao

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2331068

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001627621 – 345D-amino-acid oxidaseAdd BLAST345

Proteomic databases

MaxQBiP18894
PaxDbiP18894
PeptideAtlasiP18894
PRIDEiP18894

PTM databases

iPTMnetiP18894
PhosphoSitePlusiP18894

Expressioni

Gene expression databases

CleanExiMM_DAO1

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP18894 2 interactors.
MINTiP18894
STRINGi10090.ENSMUSP00000107911

Chemistry databases

BindingDBiP18894

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DXOmodel-@1-336[»]
ProteinModelPortaliP18894
SMRiP18894
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi343 – 345Microbody targeting signal3

Sequence similaritiesi

Belongs to the DAMOX/DASOX family.Curated

Phylogenomic databases

eggNOGiKOG3923 Eukaryota
COG0665 LUCA
HOGENOMiHOG000046303
HOVERGENiHBG003493
InParanoidiP18894
KOiK00273
PhylomeDBiP18894
TreeFamiTF313887

Family and domain databases

InterProiView protein in InterPro
IPR006181 D-amino_acid_oxidase_CS
IPR023209 DAO
IPR006076 FAD-dep_OxRdtase
PANTHERiPTHR11530 PTHR11530, 1 hit
PfamiView protein in Pfam
PF01266 DAO, 1 hit
PIRSFiPIRSF000189 D-aa_oxidase, 1 hit
PROSITEiView protein in PROSITE
PS00677 DAO, 1 hit

Sequencei

Sequence statusi: Complete.

P18894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAVIGAGV IGLSTALCIH ERYHPTQPLH MKIYADRFTP FTTSDVAAGL
60 70 80 90 100
WQPYLSDPSN PQEAEWSQQT FDYLLSCLHS PNAEKMGLAL ISGYNLFRDE
110 120 130 140 150
VPDPFWKNAV LGFRKLTPSE MDLFPDYGYG WFNTSLLLEG KSYLPWLTER
160 170 180 190 200
LTERGVKLIH RKVESLEEVA RGVDVIINCT GVWAGALQAD ASLQPGRGQI
210 220 230 240 250
IQVEAPWIKH FILTHDPSLG IYNSPYIIPG SKTVTLGGIF QLGNWSGLNS
260 270 280 290 300
VRDHNTIWKS CCKLEPTLKN ARIVGELTGF RPVRPQVRLE REWLRHGSSS
310 320 330 340
AEVIHNYGHG GYGLTIHWGC AMEAANLFGK ILEEKKLSRL PPSHL
Length:345
Mass (Da):38,661
Last modified:April 29, 2008 - v3
Checksum:i29A5D9B55D64700F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64A → V in AAA39367 (PubMed:1976103).Curated1
Sequence conflicti64A → V in BAA01063 (PubMed:1355365).Curated1
Sequence conflicti157K → N in BAA01062 (PubMed:1355365).Curated1
Sequence conflicti171R → RG in BAA01062 (PubMed:1355365).Curated1
Sequence conflicti296H → F in AAA39367 (PubMed:1976103).Curated1
Sequence conflicti296H → F in BAA01062 (PubMed:1355365).Curated1
Sequence conflicti296H → F in BAA01063 (PubMed:1355365).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti181G → R Abolishes activity. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32299 mRNA Translation: AAA39367.1
D10210 mRNA Translation: BAA01062.1
D10211 mRNA Translation: BAA01063.1
AK134813 mRNA Translation: BAE22296.1
BC018377 mRNA Translation: AAH18377.1
CCDSiCCDS19557.1
PIRiJH0185
RefSeqiNP_001273325.1, NM_001286396.1
NP_034148.2, NM_010018.3
XP_006530224.1, XM_006530161.2
XP_006530225.1, XM_006530162.3
UniGeneiMm.20115

Genome annotation databases

GeneIDi13142
KEGGimmu:13142
UCSCiuc008yza.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiOXDA_MOUSE
AccessioniPrimary (citable) accession number: P18894
Secondary accession number(s): Q64465, Q8VCW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 29, 2008
Last modified: February 28, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome